CX294957

Overview
NameCX294957
Unique NameCX294957
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length587
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CX294957_ssr209 microsatellite CX294957_ssr209:209..220. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig5796 contig Ccv1_Contig5796:63..650. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_BACME (Glyceraldehyde-3-phosphate dehydrogenase OS=Bacillus megaterium GN=gap PE=3 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 3.734e-13
Identity = 40/87 (45.98%), Postives = 55/87 (63.22%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWG 511
            +K+ INGFGRIGRN  R     K+  +EVVAIND       +HLLKYDS  G  +A+V   G++ + V+GK I++ + R+P  L WG
Sbjct:    3 VKIGINGFGRIGRNVFRA--ALKNDNVEVVAINDLTDANMLAHLLKYDSVHGKLDAEVVVDGSN-LVVNGKTIEISAERDPAQLSWG 86
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P2_BACSU (Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Bacillus subtilis GN=gapB PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 4.260e-13
Identity = 37/90 (41.11%), Postives = 58/90 (64.44%), Query Frame = 2 
Query:  248 KLKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGDW 517
            K+KVAINGFGRIGR   R      D  ++VVAIN +   +  +HL+KYD+  G ++ +V   G D + V+GK + ++++R+P  LPW ++
Sbjct:    2 KVKVAINGFGRIGRMVFR--KAMLDDQIQVVAINASYSAETLAHLIKYDTIHGRYDKEV-VAGEDSLIVNGKKVLLLNSRDPKQLPWREY 88

HSP 2 Score: 23.8682 bits (50), Expect = 4.260e-13
Identity = 10/21 (47.62%), Postives = 13/21 (61.90%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVF 552
            P+Q P     ID+V+E TG F
Sbjct:   80 PKQLPWREYDIDIVVEATGKF 100
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_BUCBP (Glyceraldehyde-3-phosphate dehydrogenase OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=gapA PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 4.262e-13
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            +K+ INGFGRIGR   R    R  S +E++AIND   V+  +++LKYDS  G F   +  +  + + V+GK I++ + ++P NL WGD
Sbjct:    3 IKIGINGFGRIGRVLFRLAQLR--SNIEILAINDLINVQYIAYMLKYDSIHGNFNGKI-VIKNETLFVNGKKIRITNEKDPKNLMWGD 87

HSP 2 Score: 27.7202 bits (60), Expect = 4.262e-13
Identity = 12/25 (48.00%), Postives = 16/25 (64.00%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVFVDRE 564
            P+    G L ID+VIE TG+F+  E
Sbjct:   80 PKNLMWGDLNIDVVIESTGIFLTTE 104
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_DEBHA (Glyceraldehyde-3-phosphate dehydrogenase OS=Debaryomyces hansenii GN=GPD PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 5.536e-13
Identity = 35/88 (39.77%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWG 511
            + + INGFGRIGR  LR    R D  ++VVA+ND     + A+++ KYDST G ++ +V     D + +DG+ I+V   ++P ++PWG
Sbjct:    3 ITIGINGFGRIGRLVLRIALQRSD--IKVVAVNDPFIAPEYAAYMFKYDSTHGRYKGEVTSKD-DSLVIDGQSIKVFGEKDPASIPWG 87

HSP 2 Score: 31.187 bits (69), Expect = 5.536e-13
Identity = 14/26 (53.85%), Postives = 15/26 (57.69%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVFVDREG 567
            P   P G  G+D VIE TGVF   EG
Sbjct:   81 PASIPWGKAGVDYVIESTGVFTTTEG 106
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P3_KLUMA (Glyceraldehyde-3-phosphate dehydrogenase 3 OS=Kluyveromyces marxianus GN=GAP3 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 5.537e-13
Identity = 42/88 (47.73%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWG 511
            +++AINGFGRIGR  LR    RK+  +EVVAIND    V  A+++ KYDST G F+ +V   G   + +DGK + V   R+P  LPWG
Sbjct:    2 VRIAINGFGRIGRLVLRIALSRKN--IEVVAINDPFITVDYAAYMFKYDSTHGRFDGEVSHDG-KALIIDGKKVLVFQERDPATLPWG 86

HSP 2 Score: 22.7126 bits (47), Expect = 5.537e-13
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVF 552
            P   P G   ID+ I+ TG+F
Sbjct:   80 PATLPWGAEKIDIAIDSTGIF 100
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P2_KLUMA (Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Kluyveromyces marxianus GN=GAP2 PE=3 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 5.537e-13
Identity = 42/88 (47.73%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWG 511
            +++AINGFGRIGR  LR    RK+  +EVVAIND    V  A+++ KYDST G F+ +V   G   + +DGK + V   R+P  LPWG
Sbjct:    2 VRIAINGFGRIGRLVLRIALSRKN--IEVVAINDPFITVDYAAYMFKYDSTHGRFDGEVSHDG-KSLIIDGKKVLVFQERDPATLPWG 86

HSP 2 Score: 22.7126 bits (47), Expect = 5.537e-13
Identity = 9/21 (42.86%), Postives = 12/21 (57.14%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVF 552
            P   P G   ID+ I+ TG+F
Sbjct:   80 PATLPWGAEKIDIAIDSTGIF 100
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P1_SALTY (Glyceraldehyde-3-phosphate dehydrogenase OS=Salmonella typhimurium GN=gapA PE=3 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 5.537e-13
Identity = 40/88 (45.45%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            +KV INGFGRIGR   R    +K S +E+VAIND    +  +++LKYDST G F+  V+ V    + V+GK I+V + R+P NL W +
Sbjct:    3 IKVGINGFGRIGRIVFRA--AQKRSDIEIVAINDLLDAEYMAYMLKYDSTHGRFDGTVE-VKDGHLIVNGKKIRVTAERDPANLKWDE 87

HSP 2 Score: 23.0978 bits (48), Expect = 5.537e-13
Identity = 8/17 (47.06%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:  514 LGIDLVIEGTGVFVDRE 564
            +G+D+V E TG+F+  E
Sbjct:   88 VGVDVVAEATGIFLTDE 104
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P1_SALTI (Glyceraldehyde-3-phosphate dehydrogenase OS=Salmonella typhi GN=gapA PE=3 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 5.537e-13
Identity = 40/88 (45.45%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            +KV INGFGRIGR   R    +K S +E+VAIND    +  +++LKYDST G F+  V+ V    + V+GK I+V + R+P NL W +
Sbjct:    3 IKVGINGFGRIGRIVFRA--AQKRSDIEIVAINDLLDAEYMAYMLKYDSTHGRFDGTVE-VKDGHLIVNGKKIRVTAERDPANLKWDE 87

HSP 2 Score: 23.0978 bits (48), Expect = 5.537e-13
Identity = 8/17 (47.06%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:  514 LGIDLVIEGTGVFVDRE 564
            +G+D+V E TG+F+  E
Sbjct:   88 VGVDVVAEATGIFLTDE 104
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_THEMA (Glyceraldehyde-3-phosphate dehydrogenase OS=Thermotoga maritima GN=gap PE=1 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 6.370e-13
Identity = 42/87 (48.28%), Postives = 53/87 (60.92%), Query Frame = 2 
Query:  254 KVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            +VAINGFGRIGR   R  + RK+  +EVVAIND    K  +HLLKYDS    F   V+    + + VDGK I+V +  +P  LPW D
Sbjct:    3 RVAINGFGRIGRLVYRIIYERKNPDIEVVAINDLTDTKTLAHLLKYDSVHKKFPGKVE-YTENSLIVDGKEIKVFAEPDPSKLPWKD 88
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P1_KLUMA (Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Kluyveromyces marxianus GN=GAP1 PE=1 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 7.194e-13
Identity = 38/88 (43.18%), Postives = 57/88 (64.77%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWG 511
            + +AINGFGRIGR  LR    RK+  ++VVAIND    V  A+++ KYDST G ++ +V   G++ + ++GK + V   ++P  LPWG
Sbjct:    2 VSIAINGFGRIGRLVLRIALERKN--IDVVAINDPFISVDYAAYMFKYDSTHGKYKGEVSHDGSN-LIINGKKVAVFQEKDPATLPWG 86

HSP 2 Score: 27.335 bits (59), Expect = 7.194e-13
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVF 552
            P   P G LG+D+ ++ TGVF
Sbjct:   80 PATLPWGKLGVDIAVDSTGVF 100
The following BLAST results are available for this feature:
BLAST of CX294957 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 114
Match NameE-valueIdentityDescription
G3P_BACME3.734e-1345.98Glyceraldehyde-3-phosphate dehydrogenase OS=Bacill... [more]
G3P2_BACSU4.260e-1341.11Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Baci... [more]
G3P_BUCBP4.262e-1340.91Glyceraldehyde-3-phosphate dehydrogenase OS=Buchne... [more]
G3P_DEBHA5.536e-1339.77Glyceraldehyde-3-phosphate dehydrogenase OS=Debary... [more]
G3P3_KLUMA5.537e-1347.73Glyceraldehyde-3-phosphate dehydrogenase 3 OS=Kluy... [more]
G3P2_KLUMA5.537e-1347.73Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Kluy... [more]
G3P1_SALTY5.537e-1345.45Glyceraldehyde-3-phosphate dehydrogenase OS=Salmon... [more]
G3P1_SALTI5.537e-1345.45Glyceraldehyde-3-phosphate dehydrogenase OS=Salmon... [more]
G3P_THEMA6.370e-1348.28Glyceraldehyde-3-phosphate dehydrogenase OS=Thermo... [more]
G3P1_KLUMA7.194e-1343.18Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Kluy... [more]

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Properties
Property NameValue
Genbank descriptionC05065A08SK DroRLeaf1 Citrus clementina cDNA clone C05065A08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX294957 ID=CX294957; Name=CX294957; organism=Citrus clementina; type=EST; length=587bp
GAGGCCACTGCTTGCTCTTGCTTGCATCATCCAGCAAATACCTATCATGG
CTTCGGCCACTCTCTCTGTAGCCAAATCAGCTCTTCAGGGAAATGGTAAG
GGATTCTCAGAATTCTCAGGTCTACGCAACTCTGCAAGCCTTCCTTTTGG
CAGGAAATCTTCTGATGACTTCCATTCAGTCATTGCTCTCCAGACCTCTG
CTTTAGGAAGCAGCAGCAGCGGGTACAGGAAGGTTGCTGCTCAGGCAAAG
CTGAAGGTTGCAATCAATGGGTTTGGTAGAATTGGTAGGAACTTCTTGAG
GTGCTGGCACGGCCGCAAGGACTCTCCACTTGAAGTCGTTGCCATCAATG
ACACCGGAGGTGTCAAGCAGGCCTCTCACCTCCTCAAGTACGACTCCACC
CTTGGCATCTTTGAAGCCGATGTTAAACCAGTTGGTACTGATGGCATCTC
TGTTGATGGCAAGGTCATCCAGGTTGTTTCTAACCGCAACCCCGTCAATC
TCCCCTGGGGGGACTGGGCATCGACCTTGTGATTGAAGGGACAGGAGTGT
TTGTAGACAGAGAGGGCGGCAGCACATCAGCTGTGCA
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