CX295016

Overview
NameCX295016
Unique NameCX295016
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length706
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_SERP5 (Soluble pyridine nucleotide transhydrogenase OS=Serratia proteamaculans (strain 568) GN=sthA PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.980e-16
Identity = 60/231 (25.97%), Postives = 104/231 (45.02%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIP--GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L +  S   E      Y       +   +  +L+   + I +   + +        KL+ G+ + +  N V V+ +DGT+ +  A HI+IA GSR                SD  L L   P+  ++ G G I  E+ASI+RG+   VDL+  ++  L   D EM   ++ +    G+ +      +++  +++GV      G+++ AD +L+A GR
Sbjct:   42 GGGCTHWGTIPSKALRHAVSRIIEFNQNPLYNNS--RTLSATFPDILRHADNVINQQTRMRQGFYERNQCKLFAGDARFIDANTVSVSYMDGTQDTIRADHIVIACGSRPYNPSSVDFNHPRIYNSDSILELNHEPRHVIIYGAGVIGCEYASIFRGLNVKVDLINTRDRLLAFLDQEMSDSLSYHFWNNGVVIRHNEEFEKIEGTDDGVIVHLKSGKKVKADCLLYANGR 270          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_PSEAE (Mercuric reductase OS=Pseudomonas aeruginosa GN=merA PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.980e-16
Identity = 71/237 (29.96%), Postives = 106/237 (44.73%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLL---QKKTDEILRLNGIYKRLLS-NAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAI--TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCV  GCVP KI++  A       ++   G         D  KLL   Q + DE+      Y+ +L  N  + +  GE +      + V   +G +        L+ATG+     PIPG + +   TS EAL+ + +P+R  V+G   +A+E A  +  +GS V +L R  L  R  D  +   V       GI +   T   ++   +      T HG E+ AD +L ATGR P
Sbjct:  133 GGTCVNVGCVPSKIMIRAAHIAHLRRESPFDGGIAATVPTIDRSKLLAQQQARVDELRHAK--YEGILGGNPAITVVHGEARFKDDQSLTVRLNEGGERVVMFDRCLVATGASPAVPPIPGLKESPYWTSTEALASDTIPERLAVIGSSVVALELAQAFARLGSKVTVLARNTLFFRE-DPAIGEAVTAAFRAEGIEVLEHTQASQVAHMDGEFVLTTTHG-ELRADKLLVATGRTP 365          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_ALCSP (Mercuric reductase OS=Alcaligenes sp. GN=merA PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.980e-16
Identity = 73/237 (30.80%), Postives = 107/237 (45.15%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLL---QKKTDEILRLNGIYKRLLS-NAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAI--TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCV  GCVP KI++  A       ++   G         D  KLL   Q + DE+      Y+ +L  N  + +  GE +      + V   DG +   +    L+ATG+     PIPG + +   TS EAL  + +P+R  V+G   +A+E A  +  +GS V +L R  L  R  D  +   V       GI +   T   ++   +      T +G EI AD +L ATGRAP
Sbjct:  131 GGTCVNVGCVPSKIMIRAAHIAHLRRESPFDGGIAATVPAIDRSKLLAQQQARVDELRHAK--YEGILDDNPAITVLHGEARFKDDQSLAVRLNDGGERVVAFDRCLVATGASPAVPPIPGLKESPYWTSTEALVSDTIPERLAVIGSSVVALELAQAFARLGSKVTILARSTLFFRE-DPAIGEAVTAAFRAEGIKVLEYTQASQVAHVDGEFVLTTGYG-EIRADQLLVATGRAP 363          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_VIBSL (Soluble pyridine nucleotide transhydrogenase OS=Vibrio splendidus (strain LGP32) GN=sthA PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.198e-16
Identity = 62/235 (26.38%), Postives = 110/235 (46.81%), Query Frame = 1
Query:    1 DSAEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG--QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            +S+ GG C   G +P K L +  S   E      +  + ++ I   +  +L      I +   + +         L  G  + +  + + V Q DGT+  YSA   +IATGSR  +        E    SD  LSL+  P+  ++ G G I  E+ASI+RG+G   DL+  ++  L   D+E    ++ +    G+ +    T +++  +++GV    + G+++ AD +L+A GR
Sbjct:   38 ESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLF-CQNNKSIHSTFSNILGHAKSVIDKQTRLRQGFYDRNQCTLVFGTARFIDTHTISVMQSDGTEEHYSADKFVIATGSRPYQPDNVDFMHERVYDSDSILSLKHDPQHIIIYGAGVIGCEYASIFRGLGVKTDLINTRDRLLSFLDNETSDALSYHFWNSGVVIRNDETFEKIEGTDDGVIIHLESGKKMRADCLLYANGR 271          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_PSEFL (Mercuric reductase OS=Pseudomonas fluorescens GN=merA PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.198e-16
Identity = 72/237 (30.38%), Postives = 105/237 (44.30%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLL---QKKTDEILRLNGIYKRLLS-NAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELAI--TSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTCV  GCVP KI++  A       ++   G         D  KLL   Q + DE+      Y+ +L  N  + +  GE +      + V   +G +   +    L+ATG+     PIPG + +   TS EAL  + +P R  V+G   +A+E A  +  +GS V +L R  L  R  D  +   V       GI +   T   ++          T HG E+ AD +L ATGRAP
Sbjct:  120 GGTCVNIGCVPSKIMIRAAHIAHLRRESPFDGGIAATVPAIDRSKLLAQQQARVDELRHAK--YEGILDGNPAITVLHGEARFKDDQSLVVRLNEGGEREVTFDRCLVATGASPAVPPIPGLKESPYWTSTEALVSDTIPARLAVIGSSVVALELAQAFARLGSQVTILARSTLFFRE-DPAIGEAVTAAFRAEGIEVLEHTQASQVAHVNGEFVLTTGHG-ELRADKLLVATGRAP 352          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_SHEPU (Mercuric reductase OS=Shewanella putrefaciens GN=merA PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 6.788e-16
Identity = 74/233 (31.76%), Postives = 109/233 (46.78%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGI-YKRLL-SNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG--QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG CV  GCVP KIL+  A    +  +    G E H         L Q++T  +  L    Y+ +L +N  + L +G  +    N + V + DGT+ +  A  ILIATGS     PI G  +    TS EAL  +ELP+  VV+G   +A+E A  +R +GS V +L R  L  R  D  +   +    E  GI +   T   ++          T+ G ++  D +L +TGR
Sbjct:  130 GGCCVNVGCVPSKILIRAAQLAQQQRNNPFTGLENHAP-QLSRALLTQQQTARVEELRAAKYQNILETNPALSLLKGWAQFKNANTLIVRKNDGTEQAVHADKILIATGSTPTIPPIDGLTETPYWTSTEALFAQELPQHLVVIGSSVVALEIAQAYRRLGSEVTILARHTLLYRE-DPLLGEKLTGCFEKEGIRVLNSTQATKVTHDGSQFTLETNAG-DLRCDRLLVSTGR 359          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: IRC15_YEAST (Increased recombination centers protein 15 OS=Saccharomyces cerevisiae GN=IRC15 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 6.788e-16
Identity = 58/195 (29.74%), Postives = 93/195 (47.69%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDAR---SYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP--IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGI 582
            GG  ++ G VP K L+Y +     L+        G  +     FD +         I  L  +YKR LS   V +Y+G      P+ VE+ Q         AK+I++ATGS   + P      +  I+SD+ALSL+ +P R  ++GGG I +E A I+  +GS V ++  +    +  D+E+ +     L+ +GI
Sbjct:   53 GGAYLVDGAVPSKTLLYESYLYRLLQQQELIEQRGTRLFPA-KFDMQAAQSALKHNIEELGNVYKRELSKNNVTVYKGTAAFKDPHHVEIAQRGMKPFIVEAKYIVVATGSAVIQCPGVAIDNDKIISSDKALSLDYIPSRFTIMGGGTIGLEIACIFNNLGSRVTIVESQSEICQNMDNELASATKTLLQCQGI 246          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_RHOCA (Dihydrolipoyl dehydrogenase OS=Rhodobacter capsulatus GN=lpd PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.158e-15
Identity = 74/242 (30.58%), Postives = 120/242 (49.59%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQEL----AITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSE-EGVKRITDHGEE----IVADVVLFATGRAP 705
            A GGTC+  GC+P K L++      E+ +       +  K+  DW K+    T  I       + L     V    G G I  P +V+V    G ++ ++AK+I+IATGS +  + +PG E+     +TS  ALSL ++PK  VV+G G I +E  S++  +G+ V ++   +    G D E+   + R L  +G+       ++ + K++ +   R T   +E    I A+VVL ATGR P
Sbjct:   37 ALGGTCLNVGCIPSKALLHATHELHEVHENFEKMGLMGAKVKVDWAKI-DGNTKGI-------EFLFKKNKVTYLRGWGSIPAPGQVKV----GDEV-HTAKNIVIATGSES--SGLPGIEIDEQTVVTSTGALSLAKVPKSMVVIGAGVIGLELGSVYARLGAEVTVVEYLDAITPGMDAEVAKGLQRILTRQGLKFVLGAAVQGVDKAKGKNTVRYTLRKDESAHAIEAEVVLVATGRKP 263          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_CHLTR (Dihydrolipoyl dehydrogenase OS=Chlamydia trachomatis GN=lpdA PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.158e-15
Identity = 60/233 (25.75%), Postives = 112/233 (48.07%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELA---ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GGTC+ RGC+P K L+ GA    ++  A  +G  V E    ++  ++Q+K   +  +      L+ +  + ++ G G ++   EV++  L        A  I++ATGS  +  P IP    +   + S   L+L+E+P++  ++GGG I  EFAS++  +GS V ++      L   + ++   +      +G+      ++  +    + V R+T +G     D VL + GR
Sbjct:   39 GGTCLNRGCIPSKALLAGAEVVTQIRHADQFGIHV-EGFSINYPAMVQRKDSVVRSIRDGLNGLIRSNKITVFSGRGSLISSTEVKI--LGENPSVIKAHSIILATGSEPRAFPGIPFSAESPRILCSTGVLNLKEIPQKMAIIGGGVIGCEFASLFHTLGSEVSVIEASSQILALNNPDISKTMFDKFTRQGLRFVLEASVSNIEDIGDRV-RLTINGNVEEYDYVLVSIGR 267          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: STHA_YERPY (Soluble pyridine nucleotide transhydrogenase OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=sthA PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.975e-15
Identity = 62/232 (26.72%), Postives = 102/232 (43.97%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIP---GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGEEIVADVVLFATGR 699
            GG C   G +P K L +  S   E      Y  +    I   +  +L      I +   + +         ++ G+   +  N V V   DGT  +  A +I+IATGSR  R P+      E    SD  L L   P+  ++ G G I  E+ASI+RG+   VDL+  ++  L   D EM   ++ +    G+ +      +++  + +GV      G+++ AD +L+A GR
Sbjct:   42 GGGCTHWGTIPSKALRHAVSRIIEFNQNPLYS-DNARTIKSSFADILNHADRVINQQTRMRQGFYDRNHCHMFSGDASFIDANTVNVRYADGTSDTLQADNIVIATGSRPYR-PVNVDFNHERIYDSDTILQLSHEPQHVIIYGAGVIGCEYASIFRGLSVKVDLINTRDRLLAFLDQEMSDALSYHFWNNGVVIRHNEEFEQIEGTTDGVIVHLKSGKKVKADCLLYANGR 271          
The following BLAST results are available for this feature:
BLAST of CX295016 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
STHA_SERP53.980e-1625.97Soluble pyridine nucleotide transhydrogenase OS=Se... [more]
MERA_PSEAE3.980e-1629.96Mercuric reductase OS=Pseudomonas aeruginosa GN=me... [more]
MERA_ALCSP3.980e-1630.80Mercuric reductase OS=Alcaligenes sp. GN=merA PE=3... [more]
STHA_VIBSL5.198e-1626.38Soluble pyridine nucleotide transhydrogenase OS=Vi... [more]
MERA_PSEFL5.198e-1630.38Mercuric reductase OS=Pseudomonas fluorescens GN=m... [more]
MERA_SHEPU6.788e-1631.76Mercuric reductase OS=Shewanella putrefaciens GN=m... [more]
IRC15_YEAST6.788e-1629.74Increased recombination centers protein 15 OS=Sacc... [more]
DLDH_RHOCA1.158e-1530.58Dihydrolipoyl dehydrogenase OS=Rhodobacter capsula... [more]
DLDH_CHLTR1.158e-1525.75Dihydrolipoyl dehydrogenase OS=Chlamydia trachomat... [more]
STHA_YERPY1.975e-1526.72Soluble pyridine nucleotide transhydrogenase OS=Ye... [more]

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Properties
Property NameValue
Genbank descriptionC05065F09SK DroRLeaf1 Citrus clementina cDNA clone C05065F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295016 ID=CX295016; Name=CX295016; organism=Citrus clementina; type=EST; length=706bp
GATTCGGCCGAGGGTGGAACCTGTGTCATTCGTGGTTGTGTACCCAAAAA
GATATTGGTCTATGGAGCATCATTTGGAGGTGAACTTGAGGATGCAAGGA
GTTATGGATGGGAGGTACACGAGAAAATTGACTTCGACTGGAAAAAACTT
CTGCAAAAGAAGACAGATGAGATACTCAGATTAAATGGAATTTACAAGCG
ATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGAGGGAAAGATAG
TTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCACTAAGTTAAGT
TACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGGGCTCAACGTGC
TCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGAGGCATTGAGCT
TGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAGGGTACATTGCT
GTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACTGTGGATCTATT
ATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGAAATGCGGGCAG
TAGTTGCCAGAAATCTGGAAGGGAGGGGAATTAATTTGCACCCGAGGACT
ACCATAAAGGAGTTGATTAAGTCAGAGGAGGGTGTAAAACGTATTACAGA
TCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCCACTGGCAGGG
CACCTA
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