CX295016

Overview
NameCX295016
Unique NameCX295016
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length706
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_MANSE (Dihydrolipoyl dehydrogenase OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.039e-20
Identity = 80/246 (32.52%), Postives = 123/246 (50.00%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGAS-FGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLL-FRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITD---------HGEEIVADVVLFATGRAP 705
            GGTC+  GC+P K L++    +     D +  G E  E + F++K ++  K + +  L G    L     VKL  G G IV PN+VEV    G + + + K+ILIATGS     P PG    ++  ITS  ALSLE +PK+ +V+G G I +E  S+++ +G+ V  + F   +   G D E+ +   R L  +G+     T +   +K E    ++ D         + E +  DVVL + GR P
Sbjct:   66 GGTCLNVGCIPSKALLHNTHLYHMAKHDFKHRGIETGE-VKFNFKAMMDYKVNAVKALTGGIAMLFQKNKVKLVRGAGTIVAPNKVEVKGEKGVE-TVNTKNILIATGSEV--TPFPGVTFDEKQIITSTGALSLESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLGSIGGIGIDMEV-SKDYRILAKQGMKFKLETKVLG-VKKEGSTVKVEDVSIEGAKGGNKETMDCDVVLISIGRRP 305          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_AZOVI (Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.039e-20
Identity = 70/240 (29.17%), Postives = 121/240 (50.42%), Query Frame = 1
Query:    4 SAEGGTCVIRGCVPKKILVYGA-SFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSR-AQRAPIP-GQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGIN--LHPRTTIKELIKSEEGVKRITDHGEEIVA-DVVLFATGRAP 705
            +A GGTC+  GC+P K L+  +  F    E  + +G    E +  D   ++ +K   +  L G    L+   GV L+EG GK++   +VEVT  DG+      +++++A+GS+  +  P P  Q++ + S  AL  + +P +  V+G G I +E  S+W  +G+ V +L   +  L   D+++     + L  +G+   L  R T  E+   +  VK +   GE+  A D ++ A GR P
Sbjct:   43 TALGGTCLNVGCIPSKALLDSSYKFHEAHESFKLHGISTGE-VAIDVPTMIARKDQIVRNLTGGVASLIKANGVTLFEGHGKLLAGKKVEVTAADGSSQVLDTENVILASGSKPVEIPPAPVDQDVIVDSTGALDFQNVPGKLGVIGAGVIGLELGSVWARLGAEVTVLEAMDKFLPAVDEQVAKEAQKILTKQGLKILLGARVTGTEVKNKQVTVKFVDAEGEKSQAFDKLIVAVGRRP 281          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_YEAST (Dihydrolipoyl dehydrogenase, mitochondrial OS=Saccharomyces cerevisiae GN=LPD1 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.478e-20
Identity = 71/249 (28.51%), Postives = 122/249 (49.00%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELE-DARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHIL------IATGSRAQRAPIPG----QELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEE-GVKRI------TDHGEEIVADVVLFATGRAP 705
            GGTC+  GC+P K L+  +    ++  +A+  G +V+  I  +     + K D + +L G  + L     V  Y+G G      ++ VT +DG + +    HIL      +ATGS     P PG    +E  ++S  ALSL+E+PKR  ++GGG I +E  S++  +GS V ++  +       D E+     + L+ +G++    T +    ++++  V  I      T+  E + A+V+L A GR P
Sbjct:   62 GGTCLNVGCIPSKALLNNSHLFHQMHTEAQKRGIDVNGDIKINVANFQKAKDDAVKQLTGGIELLFKKNKVTYYKGNGSFEDETKIRVTPVDGLEGTVKEDHILDVKNIIVATGSEV--TPFPGIEIDEEKIVSSTGALSLKEIPKRLTIIGGGIIGLEMGSVYSRLGSKVTVVEFQPQIGASMDGEVAKATQKFLKKQGLDFKLSTKVISAKRNDDKNVVEIVVEDTKTNKQENLEAEVLLVAVGRRP 308          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH_CHLTE (Dihydrolipoyl dehydrogenase OS=Chlorobium tepidum GN=lpd PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.478e-20
Identity = 68/210 (32.38%), Postives = 105/210 (50.00%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP--IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGV 636
            GG CV  GC+P K L+  A      ++  ++G  V   + FD  + +++  +  L+ +     LL  A V++  GE  + G   V VT  DG+     AK+I++ATGS  +  P   P  +  ITS EAL L+E+PK  +V+GGG I VE A  +   GS V ++      L   + E+   + R+ E  GI +H    +  +  SE GV
Sbjct:   45 GGVCVNWGCIPTKALLRSAEVFDLAKNPETFGVNVGN-VSFDLAQAVKRSRNVALKSSKGVAYLLKKAAVEVLAGEAVLTGGAGVMVTMPDGSVRMLGAKNIIVATGSTPRVIPGLEPDGKKIITSREALILKEVPKSMIVVGGGAIGVEMAWFYAKAGSKVTIVELMPRMLPAEEAEVSEALKRSFEKAGITVHCGAKLDNVAVSESGV 253          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_STAES (Mercuric reductase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=merA PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.945e-19
Identity = 72/237 (30.38%), Postives = 115/237 (48.52%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGI-YKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELA--ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVK-RITDHGEE--IVADVVLFATGRAP 705
            GGTCV  GCVP K ++      G  ++    G +       D  +L ++K   + ++    Y  L+   G  L  GE   +    ++V   + T  S+     LIATG+      IPG      +TS  AL L+E+P+R  V+G GYIA E   ++  +G+ V L+ R E   + +D E+   +  +L  +G+NL    T +++ ++ +     I  +G+E  I AD VL ATGR P
Sbjct:  120 GGTCVNIGCVPSKTMLRAGEINGLAQNNPFTGLQTSTGAA-DLAQLTEQKDGLVSQMRQEKYIDLIEEYGFDLIRGEASFIDDKTIQVNGQNITSKSF-----LIATGASPAVPEIPGMNEVDYLTSTSALELKEVPQRLAVIGSGYIAAELGQMFHNLGTEVTLMQRSERLFKTYDPEISEAIDESLTEQGLNLITGVTYQKVEQNGKSTSIYIEVNGQEQVIEADQVLVATGRKP 350          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_STAAU (Mercuric reductase OS=Staphylococcus aureus GN=merA PE=3 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.945e-19
Identity = 72/237 (30.38%), Postives = 115/237 (48.52%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGI-YKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELA--ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVK-RITDHGEE--IVADVVLFATGRAP 705
            GGTCV  GCVP K ++      G  ++    G +       D  +L ++K   + ++    Y  L+   G  L  GE   +    ++V   + T  S+     LIATG+      IPG      +TS  AL L+E+P+R  V+G GYIA E   ++  +G+ V L+ R E   + +D E+   +  +L  +G+NL    T +++ ++ +     I  +G+E  I AD VL ATGR P
Sbjct:  120 GGTCVNIGCVPSKTMLRAGEINGLAQNNPFTGLQTSTGAA-DLAQLTEQKDGLVSQMRQEKYIDLIEEYGFDLIRGEASFIDDKTIQVNGQNITSKSF-----LIATGASPAVPEIPGMNEVDYLTSTSALELKEVPQRLAVIGSGYIAAELGQMFHNLGTEVTLMQRSERLFKTYDPEISEAIDESLTEQGLNLITGVTYQKVEQNGKSTSIYIEVNGQEQVIEADQVLVATGRKP 350          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH2_PSEPU (Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4)

HSP 1 Score: 95.1301 bits (235), Expect = 3.846e-19
Identity = 70/239 (29.29%), Postives = 116/239 (48.54%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSR---AQRAPIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNL--EGRGINLHPRTTIKELIKSEEGVKRITDHGEE-IVADVVLFATGRAP 705
            A GGTC+  GC+P K L+  +    E +++ +       ++  D   ++ +K   +  L G    L    GV   +G GK++   +VEVT+ DGT     A+++++A+GSR      AP+  Q + + S  AL  + +PKR  V+G G I +E  S+W  +G+ V +L   +  L   D  +     + L  +G  I L  R T  ++  +E  V      GE+ I  D ++ A GR P
Sbjct:   44 ALGGTCLNVGCIPSKALLDSSWKYKEAKESFNVHGISTGEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQGHGKLLAGKKVEVTKADGTTEVIEAENVILASGSRPIDIPPAPV-DQNVIVDSTGALEFQAVPKRLGVIGAGVIGLELGSVWARLGAEVTVLEALDTFLMAADTAVSKEAQKTLTKQGLDIKLGARVTGSKVNGNEVEVTYTNAEGEQKITFDKLIVAVGRRP 281          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: MERA_STRLI (Mercuric reductase OS=Streptomyces lividans GN=merA PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.023e-19
Identity = 71/235 (30.21%), Postives = 110/235 (46.81%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGI-YKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAPIPGQELA--ITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRI---TDHGEEIVADVVLFATGR 699
            GGTCV  GCVP K L+  A      + A  +      +   D+  L+  K   + +L    Y  L +  G ++  G         +EV   DG   +  A H LIATGS      I G +    +TS  A+ L++LP+  ++LGGGY+ +E A ++  +GS V L  R  L  R  + E+ A +       GI +H RT ++ + +  EG+       D  +++ A  +L ATGR
Sbjct:   42 GGTCVNVGCVPSKALLAAAEARHGAQAASRFPGIQATEPALDFPALISGKDTLVGQLRAEKYTDLAAEYGWQIVHGTATFADGPMLEVALNDGGTATVEAAHYLIATGSAPTAPHIDGLDQVDYLTSTTAMELQQLPEHLLILGGGYVGLEQAQLFARLGSRVTLAVRSRLASRE-EPEISAGIENIFREEGITVHTRTQLRAVRRDGEGILATLTGPDGDQQVRASHLLIATGR 275          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH1_PSEPU (Dihydrolipoyl dehydrogenase OS=Pseudomonas putida GN=lpdV PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 8.567e-19
Identity = 77/241 (31.95%), Postives = 119/241 (49.38%), Query Frame = 1
Query:    7 AEGGTCVIRGCVPKKILVYGASF---GGELEDARSYGWEVHEKIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQRAP-IPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIKSEEGVKRITDHGE----EIVADVVLFATGRAP 705
            A GGTC+  GC+P K L++ A          +    G  V      D  + +  K   + RL      LL   GVK+  G  K++   +VEV   DG ++    +H+L+ATGS +   P +P     I+S EAL+ + LP+  VV+GGGYI +E    +R +G+ V ++  +E  L  +D E+ A VA +L+  GI LH    +   ++  E    + + G+     + AD VL A GR P
Sbjct:   39 ALGGTCLNIGCIPSKALIHVAEQFHQASRFTEPSPLGISVASP-RLDIGQSVAWKDGIVDRLTTGVAALLKKHGVKVVHGWAKVLDGKQVEV---DGQRIQ--CEHLLLATGSSSVELPMLPLGGPVISSTEALAPKALPQHLVVVGGGYIGLELGIAYRKLGAQVSVVEARERILPTYDSELTAPVAESLKKLGIALH----LGHSVEGYENGCLLANDGKGGQLRLEADRVLVAVGRRP 269          
BLAST of CX295016 vs. ExPASy Swiss-Prot
Match: DLDH3_BACSU (Dihydrolipoyl dehydrogenase OS=Bacillus subtilis GN=acoL PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 2.493e-18
Identity = 63/236 (26.69%), Postives = 119/236 (50.42%), Query Frame = 1
Query:   13 GGTCVIRGCVPKKILVYGASFGGELEDARSYGWEVHE-KIDFDWKKLLQKKTDEILRLNGIYKRLLSNAGVKLYEGEGKIVGPNEVEVTQLDGTKLSYSAKHILIATGSRAQR---APIPGQELAITSDEALSLEELPKRAVVLGGGYIAVEFASIWRGMGSTVDLLFRKELPLRGFDDEMRAVVARNLEGRGINLHPRTTIKELIK-SEEGVKRITDHGEEIVADVVLFATGRAP 705
            GGTC+  GC+P K L+  A+   +++ A S+G E+    I  DW K+  +K   + +L    + L+    +++ +G    +   ++ +   +G ++   A  +LIA+GS       AP  G E  + S +ALSL E+P   V++GGG I  E+A ++  +GS V ++   +  +   D+++  +    LE  G+ +H  + +  + + ++  + +      +  AD VL A GR P
Sbjct:   35 GGTCLNEGCIPTKSLLESANVLDKIKHADSFGIELPAGAISVDWSKMQSRKQQVVSQLVQGVQYLMKKNQIQVVKGTASFLSERKLLIEGENGKEIR-EADQVLIASGSEPIELPFAPFDG-EWILDSKDALSLSEIPSSLVIVGGGVIGCEYAGLFARLGSQVTIIETADRLIPAEDEDIARLFQEKLEEDGVEVHTSSRLGRVDQTAKTAIWKSGQREFKTKADYVLVAIGRKP 268          
The following BLAST results are available for this feature:
BLAST of CX295016 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 225
Match NameE-valueIdentityDescription
DLDH_MANSE2.039e-2032.52Dihydrolipoyl dehydrogenase OS=Manduca sexta PE=2 ... [more]
DLDH_AZOVI2.039e-2029.17Dihydrolipoyl dehydrogenase OS=Azotobacter vinelan... [more]
DLDH_YEAST3.478e-2028.51Dihydrolipoyl dehydrogenase, mitochondrial OS=Sacc... [more]
DLDH_CHLTE3.478e-2032.38Dihydrolipoyl dehydrogenase OS=Chlorobium tepidum ... [more]
MERA_STAES2.945e-1930.38Mercuric reductase OS=Staphylococcus epidermidis (... [more]
MERA_STAAU2.945e-1930.38Mercuric reductase OS=Staphylococcus aureus GN=mer... [more]
DLDH2_PSEPU3.846e-1929.29Dihydrolipoamide dehydrogenase OS=Pseudomonas puti... [more]
MERA_STRLI5.023e-1930.21Mercuric reductase OS=Streptomyces lividans GN=mer... [more]
DLDH1_PSEPU8.567e-1931.95Dihydrolipoyl dehydrogenase OS=Pseudomonas putida ... [more]
DLDH3_BACSU2.493e-1826.69Dihydrolipoyl dehydrogenase OS=Bacillus subtilis G... [more]

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Properties
Property NameValue
Genbank descriptionC05065F09SK DroRLeaf1 Citrus clementina cDNA clone C05065F09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295016 ID=CX295016; Name=CX295016; organism=Citrus clementina; type=EST; length=706bp
GATTCGGCCGAGGGTGGAACCTGTGTCATTCGTGGTTGTGTACCCAAAAA
GATATTGGTCTATGGAGCATCATTTGGAGGTGAACTTGAGGATGCAAGGA
GTTATGGATGGGAGGTACACGAGAAAATTGACTTCGACTGGAAAAAACTT
CTGCAAAAGAAGACAGATGAGATACTCAGATTAAATGGAATTTACAAGCG
ATTATTATCTAATGCTGGGGTAAAATTATATGAAGGGGAGGGAAAGATAG
TTGGTCCAAATGAAGTTGAGGTCACACAACTAGATGGCACTAAGTTAAGT
TACTCAGCAAAGCACATATTGATCGCAACTGGAAGTAGGGCTCAACGTGC
TCCTATTCCTGGCCAGGAGCTGGCTATAACCTCTGACGAGGCATTGAGCT
TGGAAGAATTACCCAAGCGTGCTGTGGTGCTTGGTGGAGGGTACATTGCT
GTTGAGTTTGCTTCCATATGGCGAGGGATGGGTTCTACTGTGGATCTATT
ATTTAGAAAGGAACTTCCATTAAGAGGGTTTGATGATGAAATGCGGGCAG
TAGTTGCCAGAAATCTGGAAGGGAGGGGAATTAATTTGCACCCGAGGACT
ACCATAAAGGAGTTGATTAAGTCAGAGGAGGGTGTAAAACGTATTACAGA
TCATGGTGAAGAGATTGTTGCAGATGTTGTACTCTTTGCCACTGGCAGGG
CACCTA
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