CX295728

Overview
NameCX295728
Unique NameCX295728
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length720
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_RAT (Triosephosphate isomerase OS=Rattus norvegicus GN=Tpi1 PE=1 SV=2)

HSP 1 Score: 259.225 bits (661), Expect = 1.571e-68
Identity = 129/202 (63.86%), Postives = 150/202 (74.26%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +L   WV+LGHSERR I  ES+E +G KV +ALS+GL VIAC+GE L++REAG T  VV  QTKAIAD V  W  +VLAYEPVWAIGTGK ATP QAQEVH +LR WL  N S  +A  TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHIFGESDELIGQKVNHALSEGLGVIACIGEKLDEREAGITEKVVFEQTKAIADNVKDWCKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKCNVSEGVAQCTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_MOUSE (Triosephosphate isomerase OS=Mus musculus GN=Tpi1 PE=1 SV=3)

HSP 1 Score: 258.84 bits (660), Expect = 2.052e-68
Identity = 126/202 (62.38%), Postives = 153/202 (75.74%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +L   WV+LGHSERR +  ES+E +G KV++AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N +  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVLGHSERRHVFGESDELIGQKVSHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVNDGVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_GORGO (Triosephosphate isomerase OS=Gorilla gorilla gorilla GN=TPI1 PE=3 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 3.500e-68
Identity = 126/202 (62.38%), Postives = 151/202 (74.75%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L P   VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK I D V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVITDNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_CANFA (Triosephosphate isomerase OS=Canis familiaris GN=TPI1 PE=1 SV=3)

HSP 1 Score: 256.529 bits (654), Expect = 1.018e-67
Identity = 126/202 (62.38%), Postives = 151/202 (74.75%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            ++   +  L     VAAQNC+    GAFTGEIS  M+ +    WV+LGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTK IAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL +N S  +A +TRIIYGGSV GA CKELA+QPDVDGFLVGGASLKPEF+DII + +
Sbjct:   48 YIDFARQKLDAKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIC_FRAAN (Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa GN=TPI PE=2 SV=1)

HSP 1 Score: 256.144 bits (653), Expect = 1.330e-67
Identity = 128/205 (62.44%), Postives = 152/205 (74.15%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLK-PEFIDIIKSAELK 671
            +L  VKSSL     ++ QN WV KGGAFTGEIS E L ++   WVILGHSERR ++ E ++F+G K AYAL++GL VIAC+GE LE+REAG T DV   Q KA AD V SW NIV+AYEPVWAIGTGKVA+P QAQEVH  +R+WL  N S E+A+ TRIIYGGSVNG N  ELA + D+DGFLVGGASLK PEF  I+ +   K
Sbjct:  106 YLDQVKSSLTDRIEISGQNSWVAKGGAFTGEISVEQLKDIGRKWVILGHSERRHVIGEDDQFIGKKAAYALNEGLGVIACIGEKLEEREAGKTFDVCYEQLKAFADAVPSWENIVVAYEPVWAIGTGKVASPQQAQEVHVAVREWLKKNVSAEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNAVTSK 310          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_XENTR (Triosephosphate isomerase OS=Xenopus tropicalis GN=tpi1 PE=2 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.269e-67
Identity = 126/202 (62.38%), Postives = 149/202 (73.76%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            +L   +  L   F V+AQNC+    GAFTGEIS  M+ +    WVILGHSERR +  ES+E +G KVA+ALS+ + VI C+GE L+QREAG T  VV  QTKAIAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR+WL  N S ++A + RIIYGGSV G  CKEL AQPD+DGFLVGGASLKPEFIDII + +
Sbjct:   47 YLDFARQKLDAKFAVSAQNCYKVAKGAFTGEISPAMIKDCGATWVILGHSERRHVFGESDELIGQKVAHALSENVGVIGCIGEKLDQREAGITEKVVFEQTKAIADNVKDWSKVVLAYEPVWAIGTGKTATPEQAQEVHKKLREWLKTNVSEDVAKSVRIIYGGSVTGGTCKELGAQPDIDGFLVGGASLKPEFIDIINAKQ 248          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPISB_DANRE (Triosephosphate isomerase B OS=Danio rerio GN=tpi1b PE=2 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.269e-67
Identity = 127/200 (63.50%), Postives = 150/200 (75.00%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            +L   +S L P+  VAAQNC+    GAFTGEIS  M+ +  + WVILGHSERR +  ES+E +G KVA+AL  GL VIAC+GE L++REAG T  VV AQTK IAD V  WS +VLAYEPVWAIGTGK A+P QAQEVH +LR+WL  N S  +A + RIIYGGSV G  CKELA+Q D+DGFLVGGASLKPEFIDII +
Sbjct:   47 YLDYARSKLDPNIDVAAQNCYKVAKGAFTGEISPAMIKDCGVKWVILGHSERRHVFGESDELIGQKVAHALENGLGVIACIGEKLDEREAGITEKVVFAQTKFIADNVKDWSKVVLAYEPVWAIGTGKTASPQQAQEVHDKLRQWLKTNVSEAVANSVRIIYGGSVTGGTCKELASQKDLDGFLVGGASLKPEFIDIINA 246          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_CHICK (Triosephosphate isomerase OS=Gallus gallus GN=TPI1 PE=1 SV=2)

HSP 1 Score: 254.988 bits (650), Expect = 2.963e-67
Identity = 127/200 (63.50%), Postives = 151/200 (75.50%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            +L   +  L     VAAQNC+    GAFTGEIS  M+ ++   WVILGHSERR +  ES+E +G KVA+AL++GL VIAC+GE L++REAG T  VV  QTKAIAD V  WS +VLAYEPVWAIGTGK ATP QAQEVH +LR WL ++ S  +A +TRIIYGGSV G NCKELA+Q DVDGFLVGGASLKPEF+DII +
Sbjct:   47 YLDFARQKLDAKIGVAAQNCYKVPKGAFTGEISPAMIKDIGAAWVILGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKAIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSHVSDAVAQSTRIIYGGSVTGGNCKELASQHDVDGFLVGGASLKPEFVDIINA 246          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPISA_DANRE (Triosephosphate isomerase A OS=Danio rerio GN=tpi1a PE=2 SV=1)

HSP 1 Score: 254.218 bits (648), Expect = 5.054e-67
Identity = 128/200 (64.00%), Postives = 148/200 (74.00%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            +L   +S L     VAAQNC+    GAFTGEIS  M+ +  I WVILGHSERR +  ES+E +G KVA+ L   L VIAC+GE LE+REAG+T DVV  QTK IAD V  W+ +VLAYEPVWAIGTGK A+P QAQEVH +LR WL AN S  +A + RIIYGGSV G NCKELAAQ DVDGFLVGGASLKPEF+DII +
Sbjct:   47 YLDYARSKLDQRIGVAAQNCYKVPKGAFTGEISPAMIKDCGIDWVILGHSERRHVFGESDELIGQKVAHCLESDLGVIACIGEKLEEREAGTTEDVVFEQTKVIADNVKDWTRVVLAYEPVWAIGTGKTASPEQAQEVHEKLRGWLRANVSDAVADSVRIIYGGSVTGGNCKELAAQADVDGFLVGGASLKPEFVDIINA 246          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_ORYLA (Triosephosphate isomerase OS=Oryzias latipes GN=tpi1 PE=3 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 9.531e-66
Identity = 124/200 (62.00%), Postives = 148/200 (74.00%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            +L   +S L     VAAQNC+    GAFTGEIS  M+ +    WVILGHSERR +  E++E +G KVA+AL   L VIAC+GE LE+REAG+T DV+ AQT+ IA+ V  W  +VLAYEPVWAIGTGK ATP QAQEVH +LR W  AN S E++ + RIIYGGSV  ANC+ELA+Q DVDGFLVGGASLKPEFIDII +
Sbjct:   47 YLDFARSGLDSRISVAAQNCYKVAKGAFTGEISPAMIKDCGADWVILGHSERRHVFGENDELIGQKVAHALESDLSVIACIGEKLEEREAGTTEDVIFAQTQVIAENVMDWEKVVLAYEPVWAIGTGKTATPEQAQEVHEKLRAWFRANISEEVSDSLRIIYGGSVTAANCRELASQTDVDGFLVGGASLKPEFIDIINA 246          
The following BLAST results are available for this feature:
BLAST of CX295728 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_RAT1.571e-6863.86Triosephosphate isomerase OS=Rattus norvegicus GN=... [more]
TPIS_MOUSE2.052e-6862.38Triosephosphate isomerase OS=Mus musculus GN=Tpi1 ... [more]
TPIS_GORGO3.500e-6862.38Triosephosphate isomerase OS=Gorilla gorilla goril... [more]
TPIS_CANFA1.018e-6762.38Triosephosphate isomerase OS=Canis familiaris GN=T... [more]
TPIC_FRAAN1.330e-6762.44Triosephosphate isomerase, chloroplastic OS=Fragar... [more]
TPIS_XENTR2.269e-6762.38Triosephosphate isomerase OS=Xenopus tropicalis GN... [more]
TPISB_DANRE2.269e-6763.50Triosephosphate isomerase B OS=Danio rerio GN=tpi1... [more]
TPIS_CHICK2.963e-6763.50Triosephosphate isomerase OS=Gallus gallus GN=TPI1... [more]
TPISA_DANRE5.054e-6764.00Triosephosphate isomerase A OS=Danio rerio GN=tpi1... [more]
TPIS_ORYLA9.531e-6662.00Triosephosphate isomerase OS=Oryzias latipes GN=tp... [more]

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Properties
Property NameValue
Genbank descriptionC05075C06SK DroRLeaf1 Citrus clementina cDNA clone C05075C06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295728 ID=CX295728; Name=CX295728; organism=Citrus clementina; type=EST; length=720bp
CTGAAGGTCAAGTTCCATCTTCTGATGTCGTTGAGGTCGTTGTTAGCCCT
CCGTTTGTGTTTCTTGGACTGGTGAAGAGTTCGTTGAGGCCTGATTTTCA
TGTTGCGGCACAGAATTGCTGGGTCAAGAAGGGAGGTGCTTTCACCGGTG
AGATTAGTGCGGAAATGCTCGTCAATTTGGATATTCCTTGGGTCATTCTT
GGTCATTCTGAAAGGAGACTCATTTTGAATGAATCAAATGAGTTTGTTGG
GGATAAGGTTGCATATGCACTTTCTCAAGGTTTGAAGGTGATTGCTTGTG
TTGGTGAGACACTTGAGCAGCGTGAAGCAGGATCTACTATGGATGTTGTT
GCTGCACAAACTAAAGCAATCGCAGATCGAGTCTCAAGCTGGTCAAACAT
TGTTTTGGCCTATGAACCTGTGTGGGCTATTGGAACTGGGAAGGTTGCCA
CTCCAGTGCAGGCTCAAGAAGTACATTTTGAACTGAGGAAATGGCTTTTT
GCAAACACCAGTCCTGAAATTGCTGCAGCAACACGGATCATCTACGGAGG
ATCCGTAAATGGTGCAAACTGCAAGGAATTGGCTGCACAGCCTGATGTTG
ACGGATTTTTGGTTGGTGGTGCTTCGTTGAAGCCGGAGTTCATTGACATC
ATCAAATCTGCTGAGCTGAAGTAAATTCTTGAGCTTTATTTCAGGGCAGT
TTGCTGATGAGTATTACTGT
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