CX295728

Overview
NameCX295728
Unique NameCX295728
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length720
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_KLUMA (Triosephosphate isomerase OS=Kluyveromyces marxianus GN=TPI1 PE=3 SV=1)

HSP 1 Score: 212.616 bits (540), Expect = 1.686e-54
Identity = 101/186 (54.30%), Postives = 132/186 (70.97%), Query Frame = 3
Query:  102 VAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            VAAQN ++K  GAFTGE S E + ++   WVILGHSERR   +E++EF+ DK  +AL  G+KVI C+GETLE+++ G T++VV  Q +A+ D+V  W+N+V+AYEPVWAIGTG  AT   AQ +H  +R++L    S + A   RI+YGGS NG N      + DVDGFLVGGASLKPEF+DII S
Sbjct:   61 VAAQNAYLKASGAFTGENSVEQIKDVGAEWVILGHSERRTYFHETDEFIADKTKFALDSGVKVILCIGETLEEKQKGITLEVVQRQLQAVLDKVQDWTNVVVAYEPVWAIGTGLAATSDDAQAIHHSIREFLAKKLSKDTAEKIRILYGGSANGKNAVTFKDKADVDGFLVGGASLKPEFVDIINS 246          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_ASHGO (Triosephosphate isomerase OS=Ashbya gossypii GN=TPI1 PE=3 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 2.876e-54
Identity = 100/190 (52.63%), Postives = 133/190 (70.00%), Query Frame = 3
Query:   90 PDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            P   V AQN + K  GA+TGE S + + ++   WVILGHSERR   NE +E V +K A+AL +G+ VI C+GETL++++AG T+DVV  Q   + ++V  W+N+V+AYEPVWAIGTG  AT   AQ++H  +R +L   TS ++A A RI++GGS NGAN      + DVDGFLVGGASLKPEF+DII S
Sbjct:   57 PQVTVGAQNAYTKASGAYTGENSVDQIKDVGAKWVILGHSERRTYFNEDDEQVAEKTAFALERGVSVILCIGETLDEKKAGVTLDVVKRQLTPVLEKVKDWTNVVIAYEPVWAIGTGLAATAEDAQDIHAAIRDFLAERTSRDVADAVRILFGGSANGANAASFRDKADVDGFLVGGASLKPEFVDIINS 246          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_YARLI (Triosephosphate isomerase OS=Yarrowia lipolytica GN=TPI1 PE=3 SV=1)

HSP 1 Score: 210.69 bits (535), Expect = 6.408e-54
Identity = 108/201 (53.73%), Postives = 137/201 (68.16%), Query Frame = 3
Query:   60 FLGLVKSSLR-PDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            +L L K SL+ P   VA QN + K  GAFTGE+S   L ++   WVILGHSERR I  ES+E++ DK  YAL  GL VI C+GET+++++AG T+DVV +Q   +  ++  WSN+V+AYEPVWAIGTG  AT   AQ++H E+R +L      + A   RIIYGGSVNG N      + DVDGFLVGGASLKPEF+DII S
Sbjct:   46 YLLLAKESLKKPTVSVAGQNSFDKGDGAFTGEVSVAQLKDVGAKWVILGHSERRTINKESSEWIADKTKYALDNGLDVILCIGETIDEKKAGKTLDVVRSQLDPVIAKIKDWSNVVIAYEPVWAIGTGLAATAEDAQQIHHEIRAYLKDKIGAQ-ADKVRIIYGGSVNGKNSGTFKDKSDVDGFLVGGASLKPEFVDIINS 245          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_KLULA (Triosephosphate isomerase OS=Kluyveromyces lactis GN=TPI1 PE=3 SV=2)

HSP 1 Score: 207.608 bits (527), Expect = 5.425e-53
Identity = 97/191 (50.79%), Postives = 133/191 (69.63%), Query Frame = 3
Query:   87 RPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            R +  ++AQN + K  GA+TGE S E + ++   WVILGHSERR   NE++E V +K  +AL  G+KVI C+GETLE+++   T+ VV  Q +A+ ++V  W+N+V+AYEPVWAIGTG  AT   AQ++H  +R++L    S ++A + RI+YGGS NG N      + DVDGFLVGGASLKPEF+DII S
Sbjct:   56 RKEVKISAQNAYSKASGAYTGENSVEQIKDVGAEWVILGHSERRTYFNETDEIVAEKTKFALDSGVKVILCIGETLEEKQQNITLQVVQRQLQAVLEKVQDWTNVVVAYEPVWAIGTGLAATAEDAQDIHHSIREFLAEKLSRDVADSVRILYGGSANGKNAVTFKDKADVDGFLVGGASLKPEFVDIINS 246          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_DICDI (Triosephosphate isomerase OS=Dictyostelium discoideum GN=tpiA PE=3 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 5.998e-52
Identity = 105/191 (54.97%), Postives = 133/191 (69.63%), Query Frame = 3
Query:   96 FHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRV-SSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASL-KPEFIDIIKSA 662
            F V +QNC+    GAFTGEISA MLV+L IP+VI+GHSERR + +ES+E +  K  YA+S GL VI C+GE L  R++ +T  +++ Q K  A      WS IV+AYEPVWAIGTG VATP +AQ+ H  +RKW+    S ++A  T I+YGGSVN  NC  L+ Q D+DGFLVGGASL   +FI IIKSA
Sbjct:   62 FKVCSQNCYSVAKGAFTGEISANMLVDLGIPYVIIGHSERRNVFSESSELITKKTKYAISLGLTVILCLGELLADRKSNNTEHILSEQLKDFASFTPEEWSKIVIAYEPVWAIGTGAVATPQEAQDTHVFIRKWISEKVSEDVAKKTSIMYGGSVNADNCHNLSKQSDIDGFLVGGASLVASDFIAIIKSA 252          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPISL_HUMAN (Putative triosephosphate isomerase-like protein LOC286016 OS=Homo sapiens PE=1 SV=2)

HSP 1 Score: 202.986 bits (515), Expect = 1.336e-51
Identity = 106/199 (53.27%), Postives = 130/199 (65.33%), Query Frame = 3
Query:   69 LVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            L +  L P   VA QNC+    GAFTGEIS  M+ +L + WV+                   KVA+AL++ L VIAC+GE L++REAG T  VV  QTK IAD V  WS ++LAY+PVWA GTGK ATP QAQEVH +LR WL +N S  +A +T IIYGGSV  A CKELA+QPDV  FL+ G SLKPEF+DII + +
Sbjct:   51 LARQKLAPKIAVAPQNCYKVTNGAFTGEISPGMVKDLGVTWVV-------------------KVAHALAERLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVILAYDPVWATGTGKTATPQQAQEVHKKLRGWLKSNISDAVAQSTGIIYGGSVTKATCKELASQPDVAAFLMSGVSLKPEFVDIINAKQ 230          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_CANGA (Triosephosphate isomerase OS=Candida glabrata GN=TPI1 PE=3 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 1.745e-51
Identity = 98/186 (52.69%), Postives = 125/186 (67.20%), Query Frame = 3
Query:  102 VAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKS 659
            V  QN + K  GA+TGE S + L ++   WVILGHSERR   +E ++ V +K  +AL QGL VI C+GETLE+++AG T+ VV  Q  A+   V  W+N+V+AYEPVWAIGTG  AT   AQ++H  +R +L      + A  TRI+YGGS NGAN      + DVDGFLVGGASLKPEF+DII S
Sbjct:   61 VGGQNTYTKASGAYTGENSVDQLKDVGAKWVILGHSERRTYFHEDDKIVAEKTKFALDQGLGVILCIGETLEEKKAGVTLKVVERQLDAVIAEVKDWTNVVIAYEPVWAIGTGLAATAEDAQDIHHSIRSYLAEKLGKDTAEKTRILYGGSANGANAGTFKDKADVDGFLVGGASLKPEFVDIINS 246          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPI2_GIALA (Triosephosphate isomerase OS=Giardia lamblia PE=3 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 6.631e-51
Identity = 100/188 (53.19%), Postives = 132/188 (70.21%), Query Frame = 3
Query:  102 VAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSS----WSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDII 653
            +AAQN +++  GA+TGE S EML+++ +  VI+GHSERR I+ E+NE    K   AL +G+ VI C GETL++R+A +TM+V  AQ +A+   +      W N+V+AYEPVW+IGTG VATP QA+EVH  LRKW       E A   RIIYGGS NG+NC++L   P++DGFLVGGASLKPEF  +I
Sbjct:   62 IAAQNVYLEGNGAWTGETSVEMLLDMGLSHVIIGHSERRRIMGETNEQSAKKAKRALDKGMTVIFCTGETLDERKANNTMEVNIAQLEALKKEIGESKKLWENVVIAYEPVWSIGTGVVATPEQAEEVHVGLRKWFAEKVCAEGAQHIRIIYGGSANGSNCEKLGQCPNIDGFLVGGASLKPEFTTMI 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPI1_GIALA (Triosephosphate isomerase OS=Giardia lamblia PE=1 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.251e-49
Identity = 99/198 (50.00%), Postives = 134/198 (67.68%), Query Frame = 3
Query:   72 VKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSS----WSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDII 653
            + ++      +AAQN +++  GA+TGE S EML ++ +  VI+GHSERR I+ E++E    K   AL +G+ VI CVGETL++R+A  TM+V  AQ +A+   +      W  +V+AYEPVW+IGTG VATP QA+EVH  LRKW       E A   RIIYGGS NG+NC++L   P++DGFLVGGASLKPEF+ +I
Sbjct:   52 IAANTSKQLRIAAQNVYLEGNGAWTGETSVEMLQDMGLKHVIVGHSERRRIMGETDEQSAKKAKRALEKGMTVIFCVGETLDERKANRTMEVNIAQLEALGKELGESKMLWKEVVIAYEPVWSIGTGVVATPEQAEEVHVGLRKWFAEKVCAEGAQHIRIIYGGSANGSNCEKLGQCPNIDGFLVGGASLKPEFMTMI 249          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_PLAFA (Triosephosphate isomerase OS=Plasmodium falciparum GN=TPI PE=1 SV=1)

HSP 1 Score: 190.274 bits (482), Expect = 8.962e-48
Identity = 96/193 (49.74%), Postives = 128/193 (66.32%), Query Frame = 3
Query:   84 LRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSA 662
            L+  F    QN      G++TGE+SAE+  +L+I +VI+GH ERR   +E++E V +K+  +L   LK + C GE+LEQRE   T++V+  Q KA  D + ++ N++LAYEP+WAIGTGK ATP QAQ VH E+RK +      + A   RI+YGGSVN  NC  L  Q D+DGFLVG ASLK  F+DIIKSA
Sbjct:   55 LQSKFSTGIQNVSKFGNGSYTGEVSAEIAKDLNIEYVIIGHFERRKYFHETDEDVREKLQASLKNNLKAVVCFGESLEQREQNKTIEVITKQVKAFVDLIDNFDNVILAYEPLWAIGTGKTATPEQAQLVHKEIRKIVKDTCGEKQANQIRILYGGSVNTENCSSLIQQEDIDGFLVGNASLKESFVDIIKSA 247          
The following BLAST results are available for this feature:
BLAST of CX295728 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_KLUMA1.686e-5454.30Triosephosphate isomerase OS=Kluyveromyces marxian... [more]
TPIS_ASHGO2.876e-5452.63Triosephosphate isomerase OS=Ashbya gossypii GN=TP... [more]
TPIS_YARLI6.408e-5453.73Triosephosphate isomerase OS=Yarrowia lipolytica G... [more]
TPIS_KLULA5.425e-5350.79Triosephosphate isomerase OS=Kluyveromyces lactis ... [more]
TPIS_DICDI5.998e-5254.97Triosephosphate isomerase OS=Dictyostelium discoid... [more]
TPISL_HUMAN1.336e-5153.27Putative triosephosphate isomerase-like protein LO... [more]
TPIS_CANGA1.745e-5152.69Triosephosphate isomerase OS=Candida glabrata GN=T... [more]
TPI2_GIALA6.631e-5153.19Triosephosphate isomerase OS=Giardia lamblia PE=3 ... [more]
TPI1_GIALA1.251e-4950.00Triosephosphate isomerase OS=Giardia lamblia PE=1 ... [more]
TPIS_PLAFA8.962e-4849.74Triosephosphate isomerase OS=Plasmodium falciparum... [more]

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Properties
Property NameValue
Genbank descriptionC05075C06SK DroRLeaf1 Citrus clementina cDNA clone C05075C06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295728 ID=CX295728; Name=CX295728; organism=Citrus clementina; type=EST; length=720bp
CTGAAGGTCAAGTTCCATCTTCTGATGTCGTTGAGGTCGTTGTTAGCCCT
CCGTTTGTGTTTCTTGGACTGGTGAAGAGTTCGTTGAGGCCTGATTTTCA
TGTTGCGGCACAGAATTGCTGGGTCAAGAAGGGAGGTGCTTTCACCGGTG
AGATTAGTGCGGAAATGCTCGTCAATTTGGATATTCCTTGGGTCATTCTT
GGTCATTCTGAAAGGAGACTCATTTTGAATGAATCAAATGAGTTTGTTGG
GGATAAGGTTGCATATGCACTTTCTCAAGGTTTGAAGGTGATTGCTTGTG
TTGGTGAGACACTTGAGCAGCGTGAAGCAGGATCTACTATGGATGTTGTT
GCTGCACAAACTAAAGCAATCGCAGATCGAGTCTCAAGCTGGTCAAACAT
TGTTTTGGCCTATGAACCTGTGTGGGCTATTGGAACTGGGAAGGTTGCCA
CTCCAGTGCAGGCTCAAGAAGTACATTTTGAACTGAGGAAATGGCTTTTT
GCAAACACCAGTCCTGAAATTGCTGCAGCAACACGGATCATCTACGGAGG
ATCCGTAAATGGTGCAAACTGCAAGGAATTGGCTGCACAGCCTGATGTTG
ACGGATTTTTGGTTGGTGGTGCTTCGTTGAAGCCGGAGTTCATTGACATC
ATCAAATCTGCTGAGCTGAAGTAAATTCTTGAGCTTTATTTCAGGGCAGT
TTGCTGATGAGTATTACTGT
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