CX295728

Overview
NameCX295728
Unique NameCX295728
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length720
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_PETHY (Triosephosphate isomerase, cytosolic OS=Petunia hybrida GN=TPIP1 PE=2 SV=1)

HSP 1 Score: 365.925 bits (938), Expect = 1.192e-100
Identity = 178/204 (87.25%), Postives = 191/204 (93.63%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            FL LVK+ LRPDFHVAAQNCWVKKGGAFTGE+SAEMLVNL IPWVILGHSERR +L ESNEFVGDKVAYALSQGLKVIACVGETLE+RE+GSTMDVVAAQTKAIADRV  W+N+V+AYEPVWAIGTGKVA+P QAQEVH ELRKWL AN SPE+AA+TRIIYGGSVNGANCKEL  QPDVDGFLVGGASLKPEFIDIIK+AE+K
Sbjct:   48 FLPLVKNELRPDFHVAAQNCWVKKGGAFTGEVSAEMLVNLSIPWVILGHSERRALLGESNEFVGDKVAYALSQGLKVIACVGETLEERESGSTMDVVAAQTKAIADRVKDWTNVVVAYEPVWAIGTGKVASPAQAQEVHAELRKWLAANVSPEVAASTRIIYGGSVNGANCKELGGQPDVDGFLVGGASLKPEFIDIIKAAEVK 251          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_ARATH (Triosephosphate isomerase, cytosolic OS=Arabidopsis thaliana GN=CTIMC PE=1 SV=2)

HSP 1 Score: 355.14 bits (910), Expect = 2.104e-97
Identity = 175/204 (85.78%), Postives = 189/204 (92.65%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            FL LVKS+LR DF VAAQNCWVKKGGAFTGE+SAEMLVNLDIPWVILGHSERR ILNES+EFVGDKVAYAL+QGLKVIACVGETLE+REAGSTMDVVAAQTKAIADRV++WSN+V+AYEPVWAIGTGKVA+P QAQEVH ELRKWL  N S ++AA TRIIYGGSVNG NCKEL  Q DVDGFLVGGASLKPEFIDIIK+AE+K
Sbjct:   48 FLPLVKSTLRSDFFVAAQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQGLKVIACVGETLEEREAGSTMDVVAAQTKAIADRVTNWSNVVIAYEPVWAIGTGKVASPAQAQEVHDELRKWLAKNVSADVAATTRIIYGGSVNGGNCKELGGQADVDGFLVGGASLKPEFIDIIKAAEVK 251          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_MAIZE (Triosephosphate isomerase, cytosolic OS=Zea mays PE=3 SV=3)

HSP 1 Score: 348.591 bits (893), Expect = 1.969e-95
Identity = 170/204 (83.33%), Postives = 184/204 (90.20%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            FL +VKS LR +FHVAAQNCWVKKGGAFTGE+SAEMLVNL +PWVILGHSERR +L ESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIA+++  WSN+V+AYEPVWAIGTGKVATP QAQEVH  LR WL  N SPE+A +TRIIYGGSV  ANCKELAAQPDVDGFLVGGASLKPEFIDII +A +K
Sbjct:   48 FLPVVKSQLRQEFHVAAQNCWVKKGGAFTGEVSAEMLVNLGVPWVILGHSERRALLGESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIAEKIKDWSNVVVAYEPVWAIGTGKVATPAQAQEVHASLRDWLKTNASPEVAESTRIIYGGSVTAANCKELAAQPDVDGFLVGGASLKPEFIDIINAATVK 251          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_ORYSJ (Triosephosphate isomerase, cytosolic OS=Oryza sativa subsp. japonica GN=TPI PE=1 SV=3)

HSP 1 Score: 345.125 bits (884), Expect = 2.177e-94
Identity = 170/204 (83.33%), Postives = 185/204 (90.69%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            FL +VKS LRP+  VAAQNCWVKKGGAFTGE+SAEMLVNL IPWVILGHSERR +L ESNEFVGDKVAYALSQGLKVIACVGETLEQRE+GSTMDVVAAQTKAI++R+  W+N+V+AYEPVWAIGTGKVATP QAQEVH  LRKWL AN S E+A +TRIIYGGSV GANCKELAA+PDVDGFLVGGASLKPEFIDII SA +K
Sbjct:   48 FLPVVKSQLRPEIQVAAQNCWVKKGGAFTGEVSAEMLVNLSIPWVILGHSERRSLLGESNEFVGDKVAYALSQGLKVIACVGETLEQRESGSTMDVVAAQTKAISERIKDWTNVVVAYEPVWAIGTGKVATPDQAQEVHDGLRKWLAANVSAEVAESTRIIYGGSVTGANCKELAAKPDVDGFLVGGASLKPEFIDIINSATVK 251          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_COPJA (Triosephosphate isomerase, cytosolic OS=Coptis japonica PE=2 SV=1)

HSP 1 Score: 345.125 bits (884), Expect = 2.177e-94
Identity = 168/204 (82.35%), Postives = 184/204 (90.20%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            FL  VK+ LR DFHVAAQNCWVKKGGAFTGE+SAEMLVNL IPWVILGHSERR +LNESNEFVGDK AYALSQGLKVIACVGETLEQREAGST+ VVAAQTKAIA++VS W+NIV+AYEPVWAIGTGKVA+P QAQEVHFELRKW+  N   ++A + RIIYGGSVNGAN KELA QPD+DGFLVGGASLKPEF+DIIKSA +K
Sbjct:   48 FLPFVKNLLRADFHVAAQNCWVKKGGAFTGEVSAEMLVNLGIPWVILGHSERRALLNESNEFVGDKTAYALSQGLKVIACVGETLEQREAGSTISVVAAQTKAIAEKVSDWTNIVVAYEPVWAIGTGKVASPAQAQEVHFELRKWIKENVGADVAGSVRIIYGGSVNGANSKELAGQPDIDGFLVGGASLKPEFVDIIKSATVK 251          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_SECCE (Triosephosphate isomerase, cytosolic OS=Secale cereale PE=2 SV=3)

HSP 1 Score: 334.339 bits (856), Expect = 3.843e-91
Identity = 163/204 (79.90%), Postives = 181/204 (88.73%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            FL  VK  LRP+  VAAQNCWVKKGGAFTGE+SAEMLVNL IPWVILGHSERR +L ES+EFVG+KVAYAL+QGLKVIACVGETLEQREAGSTM+VVA QTKAIAD++  W+N+V+AYEPVWAIGTGKVA+P QAQEVH  LR WL  N SPE+A +TRIIYGGSV GA+CKELAAQPDVDGFLVGGASLKPEFIDII +A +K
Sbjct:   48 FLPTVKDKLRPEIQVAAQNCWVKKGGAFTGEVSAEMLVNLGIPWVILGHSERRSLLAESSEFVGEKVAYALAQGLKVIACVGETLEQREAGSTMEVVAEQTKAIADKIKDWTNVVVAYEPVWAIGTGKVASPAQAQEVHANLRDWLKTNVSPEVAESTRIIYGGSVTGASCKELAAQPDVDGFLVGGASLKPEFIDIINAATVK 251          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_HORVU (Triosephosphate isomerase, cytosolic OS=Hordeum vulgare PE=1 SV=3)

HSP 1 Score: 328.946 bits (842), Expect = 1.614e-89
Identity = 161/204 (78.92%), Postives = 179/204 (87.75%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            FL +VK+ LRP+  VAAQNCWVKKGGAFTGE+SAEML NL +PWVILGHSERR +L ES+EFVG+KVAYAL+QGLKVIACVGETLEQREAGSTM+VVA QTKAIA ++  WSN V+AYEPVWAIGTGKVATP QAQEVH  LR WL  N SPE+A +TRIIYGGSV GA+CKELAAQ DVDGFLVGGASLKPEFIDII +A +K
Sbjct:   48 FLPIVKAKLRPEIQVAAQNCWVKKGGAFTGEVSAEMLANLGVPWVILGHSERRSLLGESSEFVGEKVAYALAQGLKVIACVGETLEQREAGSTMEVVAEQTKAIAGKIKDWSNGVVAYEPVWAIGTGKVATPAQAQEVHANLRDWLKTNVSPEVAESTRIIYGGSVTGASCKELAAQADVDGFLVGGASLKPEFIDIINAAAVK 251          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_LACSA (Triosephosphate isomerase, cytosolic (Fragment) OS=Lactuca sativa PE=2 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 4.697e-89
Identity = 158/190 (83.16%), Postives = 175/190 (92.11%), Query Frame = 3
Query:  102 VAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAELK 671
            VAAQNCWVKKGGAFTGE+SAEML NL +PWVILGHSERR +LNE+NEFVGDKVAYALSQGLKVIACVGETLEQREAG+TM+VVAAQTKAIAD++SSW N+VLAYEPVWAIGTGKVA+P QAQEVH  LRKW   N S E++A+TRIIYGGSV+G+NCKEL  Q DVDGFLVGGASLKPEFIDIIK+AE+K
Sbjct:    3 VAAQNCWVKKGGAFTGEVSAEMLANLGVPWVILGHSERRALLNETNEFVGDKVAYALSQGLKVIACVGETLEQREAGTTMEVVAAQTKAIADKISSWDNVVLAYEPVWAIGTGKVASPAQAQEVHAGLRKWFCDNVSAEVSASTRIIYGGSVSGSNCKELGGQTDVDGFLVGGASLKPEFIDIIKAAEVK 192          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIC_SPIOL (Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea GN=TPIP1 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 4.417e-71
Identity = 132/205 (64.39%), Postives = 154/205 (75.12%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLK-PEFIDIIKSAELK 671
            ++  VKSSL     ++AQNCW+ KGGAFTGEIS E L +L   WVILGHSERR ++ E NEF+G K AYAL+QG+ VIAC+GE LE+REAG T DV   Q KA AD + SW N+V+AYEPVWAIGTGKVA+P QAQEVH  +R WL  N S E+A+ TRIIYGGSVNG NC ELA Q D+DGFLVGGASLK PEF  I+ S   K
Sbjct:  114 YIDQVKSSLTGRVEISAQNCWIGKGGAFTGEISVEQLKDLGCQWVILGHSERRHVIGEQNEFIGKKAAYALNQGVGVIACIGELLEEREAGKTFDVCYQQLKAFADALPSWDNVVVAYEPVWAIGTGKVASPDQAQEVHVAVRDWLKKNVSEEVASKTRIIYGGSVNGGNCAELAKQEDIDGFLVGGASLKGPEFATIVNSVTAK 318          
BLAST of CX295728 vs. ExPASy Swiss-Prot
Match: TPIS_CULTA (Triosephosphate isomerase OS=Culex tarsalis GN=Tpi PE=1 SV=2)

HSP 1 Score: 265.774 bits (678), Expect = 1.678e-70
Identity = 131/202 (64.85%), Postives = 157/202 (77.72%), Query Frame = 3
Query:   60 FLGLVKSSLRPDFHVAAQNCWVKKGGAFTGEISAEMLVNLDIPWVILGHSERRLILNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGSTMDVVAAQTKAIADRVSSWSNIVLAYEPVWAIGTGKVATPVQAQEVHFELRKWLFANTSPEIAAATRIIYGGSVNGANCKELAAQPDVDGFLVGGASLKPEFIDIIKSAE 665
            +L L +S L     VAAQNC+    GAFTGEIS  ML +L+I WVILGHSERR I  ES+E + DKVA+AL++GLKVIAC+GETL++REAG T  V   QTKAIAD+V  WSN+V+AYEPVWAIGTGK A+P QAQEVH  LRKW   N S +++AA RI YGGSV  ANC+ELAA+PD+DGFLVGGASLKPEFI I+ + +
Sbjct:   46 YLTLARSQLPDSVCVAAQNCYKVPKGAFTGEISPAMLKDLNIGWVILGHSERRAIFGESDELIADKVAHALAEGLKVIACIGETLQEREAGQTEAVCFRQTKAIADKVKDWSNVVIAYEPVWAIGTGKTASPEQAQEVHAALRKWFTENVSADVSAAIRIQYGGSVTAANCRELAAKPDIDGFLVGGASLKPEFIQIVNARQ 247          
The following BLAST results are available for this feature:
BLAST of CX295728 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
TPIS_PETHY1.192e-10087.25Triosephosphate isomerase, cytosolic OS=Petunia hy... [more]
TPIS_ARATH2.104e-9785.78Triosephosphate isomerase, cytosolic OS=Arabidopsi... [more]
TPIS_MAIZE1.969e-9583.33Triosephosphate isomerase, cytosolic OS=Zea mays P... [more]
TPIS_ORYSJ2.177e-9483.33Triosephosphate isomerase, cytosolic OS=Oryza sati... [more]
TPIS_COPJA2.177e-9482.35Triosephosphate isomerase, cytosolic OS=Coptis jap... [more]
TPIS_SECCE3.843e-9179.90Triosephosphate isomerase, cytosolic OS=Secale cer... [more]
TPIS_HORVU1.614e-8978.92Triosephosphate isomerase, cytosolic OS=Hordeum vu... [more]
TPIS_LACSA4.697e-8983.16Triosephosphate isomerase, cytosolic (Fragment) OS... [more]
TPIC_SPIOL4.417e-7164.39Triosephosphate isomerase, chloroplastic OS=Spinac... [more]
TPIS_CULTA1.678e-7064.85Triosephosphate isomerase OS=Culex tarsalis GN=Tpi... [more]

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Properties
Property NameValue
Genbank descriptionC05075C06SK DroRLeaf1 Citrus clementina cDNA clone C05075C06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295728 ID=CX295728; Name=CX295728; organism=Citrus clementina; type=EST; length=720bp
CTGAAGGTCAAGTTCCATCTTCTGATGTCGTTGAGGTCGTTGTTAGCCCT
CCGTTTGTGTTTCTTGGACTGGTGAAGAGTTCGTTGAGGCCTGATTTTCA
TGTTGCGGCACAGAATTGCTGGGTCAAGAAGGGAGGTGCTTTCACCGGTG
AGATTAGTGCGGAAATGCTCGTCAATTTGGATATTCCTTGGGTCATTCTT
GGTCATTCTGAAAGGAGACTCATTTTGAATGAATCAAATGAGTTTGTTGG
GGATAAGGTTGCATATGCACTTTCTCAAGGTTTGAAGGTGATTGCTTGTG
TTGGTGAGACACTTGAGCAGCGTGAAGCAGGATCTACTATGGATGTTGTT
GCTGCACAAACTAAAGCAATCGCAGATCGAGTCTCAAGCTGGTCAAACAT
TGTTTTGGCCTATGAACCTGTGTGGGCTATTGGAACTGGGAAGGTTGCCA
CTCCAGTGCAGGCTCAAGAAGTACATTTTGAACTGAGGAAATGGCTTTTT
GCAAACACCAGTCCTGAAATTGCTGCAGCAACACGGATCATCTACGGAGG
ATCCGTAAATGGTGCAAACTGCAAGGAATTGGCTGCACAGCCTGATGTTG
ACGGATTTTTGGTTGGTGGTGCTTCGTTGAAGCCGGAGTTCATTGACATC
ATCAAATCTGCTGAGCTGAAGTAAATTCTTGAGCTTTATTTCAGGGCAGT
TTGCTGATGAGTATTACTGT
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