CX295739

Overview
NameCX295739
Unique NameCX295739
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length680
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F263_HUMAN (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 OS=Homo sapiens GN=PFKFB3 PE=1 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.443e-28
Identity = 59/100 (59.00%), Postives = 80/100 (80.00%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 302
            +EI+AGVC+ +TYEEI+   PEEY  R++DK  YRYP GESY D++QRLEPVI+ELERQ   V+VI HQAVLR L AYF D+  +E+P+++ PLHT++++
Sbjct:  322 NEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQE-NVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKL 420
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F264_MACFA (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 OS=Macaca fascicularis GN=PFKFB4 PE=2 SV=3)

HSP 1 Score: 125.946 bits (315), Expect = 1.884e-28
Identity = 58/100 (58.00%), Postives = 81/100 (81.00%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 302
            +EI+AGVC+ MTYEEI+ N P E+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +++P+++ PLHT++++
Sbjct:  325 NEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVLKL 423
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F264_HUMAN (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 OS=Homo sapiens GN=PFKFB4 PE=2 SV=6)

HSP 1 Score: 125.946 bits (315), Expect = 1.884e-28
Identity = 58/100 (58.00%), Postives = 81/100 (81.00%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 302
            +EI+AGVC+ MTYEEI+ N P E+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +++P+++ PLHT++++
Sbjct:  325 NEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTVLKL 423
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F264_RAT (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 OS=Rattus norvegicus GN=Pfkfb4 PE=1 SV=3)

HSP 1 Score: 125.176 bits (313), Expect = 3.214e-28
Identity = 58/100 (58.00%), Postives = 81/100 (81.00%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 302
            +EI+AGVC+ MTYEEI+ + P E+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +E+P+++ PLHT++++
Sbjct:  325 NEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKL 423
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F264_MOUSE (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 OS=Mus musculus GN=Pfkfb4 PE=2 SV=4)

HSP 1 Score: 125.176 bits (313), Expect = 3.214e-28
Identity = 58/100 (58.00%), Postives = 81/100 (81.00%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 302
            +EI+AGVC+ MTYEEI+ + P E+  R +DK RYRYP+GESY D++QRLEPVI+ELERQ   V+VI HQAV+R L AYF D+  +E+P+++ PLHT++++
Sbjct:  325 NEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQE-NVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKL 423
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F262_PONAB (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 OS=Pongo abelii GN=PFKFB2 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 7.161e-28
Identity = 62/114 (54.39%), Postives = 82/114 (71.93%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEIQMGVTGVQEKRYKL 344
            +EI+AGVC+ MTY EI+K  PEE+  R ++K  YRYP GESY D++QRLEPVI+ELERQ   V+VISHQAV+R L AYF D+   E+P++  PLHTI ++     G + +  KL
Sbjct:  325 NEIDAGVCEEMTYAEIEKRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQ-GNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKL 437
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F262_HUMAN (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 OS=Homo sapiens GN=PFKFB2 PE=1 SV=2)

HSP 1 Score: 124.02 bits (310), Expect = 7.161e-28
Identity = 62/114 (54.39%), Postives = 82/114 (71.93%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEIQMGVTGVQEKRYKL 344
            +EI+AGVC+ MTY EI+K  PEE+  R ++K  YRYP GESY D++QRLEPVI+ELERQ   V+VISHQAV+R L AYF D+   E+P++  PLHTI ++     G + +  KL
Sbjct:  325 NEIDAGVCEEMTYAEIEKRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQ-GNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKL 437
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F262_BOVIN (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 OS=Bos taurus GN=PFKFB2 PE=1 SV=2)

HSP 1 Score: 121.324 bits (303), Expect = 4.641e-27
Identity = 60/114 (52.63%), Postives = 82/114 (71.93%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEIQMGVTGVQEKRYKL 344
            +EI+AGVC+ MTY EI++  P+E+  R ++K  YRYP GESY D++QRLEPVI+ELERQ   V+VISHQAV+R L AYF D+   E+P++  PLHTI ++     G + +  KL
Sbjct:  326 NEIDAGVCEEMTYAEIQEQYPDEFALRDEEKYLYRYPGGESYQDLVQRLEPVIMELERQ-GNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKL 438
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: YK72_SCHPO (Probable fructose-2,6-bisphosphatase C732.02c OS=Schizosaccharomyces pombe GN=SPAC732.02c PE=2 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 6.062e-27
Identity = 55/100 (55.00%), Postives = 74/100 (74.00%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 302
            DE++AG CDG TY+ I++N P E E R  DK  YRY  GESY+DV++RLEP+I+ELERQ   V +I HQA+LR +Y Y+ +  L+E+P I  PLHTII++
Sbjct:  284 DELDAGTCDGFTYDYIEQNFPHEAELRNNDKFHYRYRGGESYMDVVRRLEPIIMELERQ-GDVFIICHQAILRCIYGYYHNLSLEELPFINVPLHTIIKL 382
[Close]
BLAST of CX295739 vs. ExPASy Swiss-Prot
Match: F262_RAT (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 OS=Rattus norvegicus GN=Pfkfb2 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 2.303e-26
Identity = 57/100 (57.00%), Postives = 76/100 (76.00%), Query Frame = 3 
Query:    3 DEINAGVCDGMTYEEIKKNMPEEYEARKKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKEIPHIETPLHTIIEI 302
            +EI+AGVC+ MTY EI++  PEE+  R ++K  YRYP GESY D++QRLEPVI+ELERQ   V+VISHQAV+R L AYF D+   E+P++  PLH I ++
Sbjct:  328 NEIDAGVCEEMTYSEIEQRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQ-GNVLVISHQAVMRCLLAYFLDKGADELPYLRCPLHIIFKL 426
The following BLAST results are available for this feature:
BLAST of CX295739 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27
Match NameE-valueIdentityDescription
F263_HUMAN1.443e-2859.006-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F264_MACFA1.884e-2858.006-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F264_HUMAN1.884e-2858.006-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F264_RAT3.214e-2858.006-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F264_MOUSE3.214e-2858.006-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F262_PONAB7.161e-2854.396-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F262_HUMAN7.161e-2854.396-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
F262_BOVIN4.641e-2752.636-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]
YK72_SCHPO6.062e-2755.00Probable fructose-2,6-bisphosphatase C732.02c OS=S... [more]
F262_RAT2.303e-2657.006-phosphofructo-2-kinase/fructose-2,6-biphosphatas... [more]

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Properties
Property NameValue
Genbank descriptionC05075D05SK DroRLeaf1 Citrus clementina cDNA clone C05075D05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX295739 ID=CX295739; Name=CX295739; organism=Citrus clementina; type=EST; length=680bp
TTGATGAGATAAATGCTGGCGTGTGTGATGGAATGACGTATGAAGAGATT
AAGAAAAATATGCCTGAGGAGTATGAGGCTCGTAAAAAGGACAAACTGAG
GTATCGGTATCCACGTGGAGAATCTTATCTTGATGTTATCCAAAGGCTAG
AACCTGTAATAATTGAACTTGAAAGACAACGAGCACCAGTTGTAGTGATA
TCTCACCAGGCGGTGTTGAGAGCTTTATATGCTTATTTTGCTGATAGGCC
TCTCAAAGAAATTCCCCACATTGAGACGCCACTCCATACAATAATCGAAA
TACAGATGGGCGTCACAGGTGTCCAAGAGAAAAGATACAAACTCATGGAC
TAAAAATTGCTTCAACGGATTCTGAAGTTCGTCGCTTTATAACTCGATGC
GACTCAACATTTGGATGGATTGAATCGCAATTCATTACTCAACTGTGTCA
TTCATTGTTACCAATGTTAATGCTAATAACCAAAATTTTATGAAGTTGTG
GGGCAAAAGCCAAGTGCTCCTCTTACTAATTAATCACATTTCGCGGTCCT
TTTTTTCCGCTACTGTTGGGCACGTCTGGGACAGTATTGAATCTAGAGGG
TCCCAAATTCAATATGTGTATGTATTAAAATATGGAAGAAATGAATAAAG
AAATAGGAATCTGTGTTTGTGTAGTGTAGG
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