CX296855

Overview
NameCX296855
Unique NameCX296855
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length694
Libraries
Library NameType
FerrChloL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig180 contig Ccv1_Contig180:154..851. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDKA_DROYA (Nucleoside diphosphate kinase (Fragment) OS=Drosophila yakuba GN=awd PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.856e-44
Identity = 70/109 (64.22%), Postives = 82/109 (75.23%), Query Frame = -3 
Query:  360 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 686
            E+TFIM+KPDGVQRGLVG+II RFE+KGF L  LK     +   EKHY DLS++PFF  L+ Y+ SGPVV M+WEG  VVKTGR ++GATNPA S PGTIRGD  I  G
Sbjct:    3 ERTFIMVKPDGVQRGLVGKIIERFEQKGFKLVALKFTWASKELLEKHYADLSARPFFPGLVNYMNSGPVVPMVWEGLNVVKTGRQMLGATNPADSLPGTIRGDFCIQVG 111

HSP 2 Score: 50.447 bits (119), Expect = 3.856e-44
Identity = 23/38 (60.53%), Postives = 28/38 (73.68%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWFPDGP-INWQSSLHPWIYE 360
            NIIHGSD+VESA+KEIALWF +   + W  +   WIYE
Sbjct:  113 NIIHGSDAVESAEKEIALWFNEKELVTWTPAAKDWIYE 150
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDK2_PEA (Nucleoside diphosphate kinase 2, chloroplastic OS=Pisum sativum GN=NDPK2 PE=1 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 4.981e-44
Identity = 72/111 (64.86%), Postives = 87/111 (78.38%), Query Frame = -3 
Query:  360 EMEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 692
            +++Q +IM+KPDGVQRGLVGEII RFEKKGF L GLKL    +  AE+HY+ L+ K FF  LIEYITSGPVV+M WEG GVV + R +IGAT+P Q+ PGTIRGD A+ TG
Sbjct:   81 QVDQAYIMVKPDGVQRGLVGEIISRFEKKGFKLTGLKLFQCSKELAEEHYKHLNQKSFFPKLIEYITSGPVVSMAWEGVGVVPSARKLIGATDPLQAEPGTIRGDFAVQTG 191

HSP 2 Score: 46.2098 bits (108), Expect = 4.981e-44
Identity = 20/38 (52.63%), Postives = 26/38 (68.42%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWFPDGPI-NWQSSLHPWIYE 360
            NIIHGSDS E+ ++EIALWF +G +  W     PW+ E
Sbjct:  193 NIIHGSDSPENGEREIALWFKEGELCEWTPVQEPWLRE 230
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDKA1_BOVIN (Nucleoside diphosphate kinase A 1 OS=Bos taurus GN=NME1-1 PE=1 SV=3)

HSP 1 Score: 142.895 bits (359), Expect = 5.022e-44
Identity = 67/109 (61.47%), Postives = 80/109 (73.39%), Query Frame = -3 
Query:  360 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 686
            E+TFI IKPDGVQRGL+GEII RFE+KGF L  +K M       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLIGEIIKRFEQKGFRLVAMKFMRASEDLLKEHYIDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 55.0694 bits (131), Expect = 5.022e-44
Identity = 26/38 (68.42%), Postives = 31/38 (81.58%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 360
            NIIHGSDSVESA+KEIALWF P+  +N++S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEIALWFHPEELVNYKSCAQNWIYE 152
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDKA_PONAB (Nucleoside diphosphate kinase A OS=Pongo abelii GN=NME1 PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 6.545e-44
Identity = 70/109 (64.22%), Postives = 81/109 (74.31%), Query Frame = -3 
Query:  360 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 686
            E+TFI IKPDGVQRGLVGEII RFE+KGF L GLK M       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 50.8322 bits (120), Expect = 6.545e-44
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 360
            NIIHGSDSVESA+KEI LWF P+  +++ S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE 152
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDKA_HUMAN (Nucleoside diphosphate kinase A OS=Homo sapiens GN=NME1 PE=1 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 6.545e-44
Identity = 70/109 (64.22%), Postives = 81/109 (74.31%), Query Frame = -3 
Query:  360 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 686
            E+TFI IKPDGVQRGLVGEII RFE+KGF L GLK M       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 50.8322 bits (120), Expect = 6.545e-44
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 360
            NIIHGSDSVESA+KEI LWF P+  +++ S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE 152
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDKB_RAT (Nucleoside diphosphate kinase B OS=Rattus norvegicus GN=Nme2 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 6.545e-44
Identity = 67/110 (60.91%), Postives = 81/110 (73.64%), Query Frame = -3 
Query:  360 MEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 689
            +E+TFI IKPDGVQRGLVGEII RFE+KGF L  +K +       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    4 LERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 55.0694 bits (131), Expect = 6.545e-44
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 360
            NIIHGSDSVESA+KEI LWF P+  I+++S  H W+YE
Sbjct:  115 NIIHGSDSVESAEKEIGLWFKPEELIDYKSCAHDWVYE 152
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDK_NEUCR (Nucleoside diphosphate kinase OS=Neurospora crassa GN=ndk-1 PE=2 SV=2)

HSP 1 Score: 150.984 bits (380), Expect = 8.529e-44
Identity = 71/109 (65.14%), Postives = 84/109 (77.06%), Query Frame = -3 
Query:  360 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 686
            EQTFI +KPDGVQRGLVG II RFE +GF L  +KL    +   EKHYEDL++KPFF  LI+Y+ SGP+ AM+WEG+  VKTGRTI+GATNP  SAPGTIRGD A+  G
Sbjct:    5 EQTFIAVKPDGVQRGLVGNIISRFENRGFKLVAMKLTQPGQAHLEKHYEDLNTKPFFAGLIKYMNSGPICAMVWEGKDAVKTGRTILGATNPLASAPGTIRGDFALDMG 113

HSP 2 Score: 46.2098 bits (108), Expect = 8.529e-44
Identity = 21/38 (55.26%), Postives = 26/38 (68.42%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWFPDGPIN-WQSSLHPWIYE 360
            N+ HGSDSVE+A+KEIALWF    +N W      WI+E
Sbjct:  115 NVCHGSDSVENAKKEIALWFKPEELNQWNHHSAAWIFE 152
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDKA2_BOVIN (Nucleoside diphosphate kinase A 2 OS=Bos taurus GN=NME1-2 PE=1 SV=3)

HSP 1 Score: 142.124 bits (357), Expect = 8.529e-44
Identity = 67/109 (61.47%), Postives = 80/109 (73.39%), Query Frame = -3 
Query:  360 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 686
            E+TFI IKPDGVQRGL+GEII RFE+KGF L  +K M       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLMGEIIKRFEQKGFRLVAMKFMRASEDLLKEHYIDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 55.0694 bits (131), Expect = 8.529e-44
Identity = 26/38 (68.42%), Postives = 31/38 (81.58%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 360
            NIIHGSDSVESA+KEIALWF P+  +N++S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEIALWFRPEELVNYKSCAQNWIYE 152
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDK2_SPIOL (Nucleoside diphosphate kinase 2, chloroplastic OS=Spinacia oleracea GN=NDPK2 PE=1 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.102e-43
Identity = 73/111 (65.77%), Postives = 89/111 (80.18%), Query Frame = -3 
Query:  360 EMEQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 692
            ++E+T+IMIKPDGVQRGLVGEII RFEKKGF L GLK+    +  AE+HY+DL +K F+  LI+YITSGPVV M WEG GVV + R +IGAT+P Q+ PGTIRGDLA+ TG
Sbjct:   84 QVEETYIMIKPDGVQRGLVGEIISRFEKKGFKLIGLKMYPCPKELAEEHYKDLKAKSFYQKLIDYITSGPVVCMAWEGVGVVASSRKLIGATDPLQAEPGTIRGDLAVQTG 194

HSP 2 Score: 43.5134 bits (101), Expect = 1.102e-43
Identity = 17/38 (44.74%), Postives = 26/38 (68.42%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWFPDGPI-NWQSSLHPWIYE 360
            N++HGSDS ++ ++EI LWF +G I  W  +  PW+ E
Sbjct:  196 NVVHGSDSPDNGKREIGLWFKEGEICQWTPAQAPWLRE 233
[Close]
BLAST of CX296855 vs. ExPASy Swiss-Prot
Match: NDKA_RAT (Nucleoside diphosphate kinase A OS=Rattus norvegicus GN=Nme1 PE=1 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 1.111e-43
Identity = 69/109 (63.30%), Postives = 81/109 (74.31%), Query Frame = -3 
Query:  360 EQTFIMIKPDGVQRGLVGEIIIRFEKKGFSLKGLKLMTVDRPFAEKHYEDLSSKPFFGSLIEYITSGPVVAMIWEGEGVVKTGRTIIGATNPAQSAPGTIRGDLAIVTG 686
            E+TFI IKPDGVQRGLVGEII RFE+KGF L GLK +       ++HY DL  +PFF  L++Y+ SGPVVAM+WEG  VVKTGR ++G TNPA S PGTIRGD  I  G
Sbjct:    5 ERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFIQASEDLLKEHYIDLKDRPFFSGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVG 113

HSP 2 Score: 51.9878 bits (123), Expect = 1.111e-43
Identity = 24/38 (63.16%), Postives = 31/38 (81.58%), Query Frame = -2 
Query:  250 NIIHGSDSVESAQKEIALWF-PDGPINWQSSLHPWIYE 360
            NIIHGSDSVESA+KEI+LWF P+  ++++S    WIYE
Sbjct:  115 NIIHGSDSVESAEKEISLWFQPEELVDYKSCAQNWIYE 152
The following BLAST results are available for this feature:
BLAST of CX296855 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
NDKA_DROYA3.856e-4464.22Nucleoside diphosphate kinase (Fragment) OS=Drosop... [more]
NDK2_PEA4.981e-4464.86Nucleoside diphosphate kinase 2, chloroplastic OS=... [more]
NDKA1_BOVIN5.022e-4461.47Nucleoside diphosphate kinase A 1 OS=Bos taurus GN... [more]
NDKA_PONAB6.545e-4464.22Nucleoside diphosphate kinase A OS=Pongo abelii GN... [more]
NDKA_HUMAN6.545e-4464.22Nucleoside diphosphate kinase A OS=Homo sapiens GN... [more]
NDKB_RAT6.545e-4460.91Nucleoside diphosphate kinase B OS=Rattus norvegic... [more]
NDK_NEUCR8.529e-4465.14Nucleoside diphosphate kinase OS=Neurospora crassa... [more]
NDKA2_BOVIN8.529e-4461.47Nucleoside diphosphate kinase A 2 OS=Bos taurus GN... [more]
NDK2_SPIOL1.102e-4365.77Nucleoside diphosphate kinase 2, chloroplastic OS=... [more]
NDKA_RAT1.111e-4363.30Nucleoside diphosphate kinase A OS=Rattus norvegic... [more]

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Properties
Property NameValue
Genbank descriptionC05803E11SK FerrChloL1 Citrus clementina cDNA clone C05803E11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX296855 ID=CX296855; Name=CX296855; organism=Citrus clementina; type=EST; length=694bp
AAGTGCAACCATCATGTGCTAACCATTAACAAACTCAATTCATAATACAG
TCAACAAAAAAGCTTAAATATTGTATATGGCAACCACAAGAATGAAAGCA
GCCAACATAACCAGGATTGGTCTCAATTTAACTCAATACTTCATAACAGT
TCACCAACAAGGAGTAACCATAAATAAGGAAATAGGGATAGGGATAGGGA
TAAGTCTAACTGCAGGAACAATTAGGCTCCTCAACCAAACATTAGTTTAC
TCATAGATCCAAGGGTGAAGGCTGCTCTGCCAGTTAATAGGGCCATCAGG
GAACCACAAGGCGATTTCTTTCTGGGCACTCTCAACTGAGTCACTTCCAT
GGATGATATTCCCGTCACGATAGCCAAATCACCACGGATGGTTCCAGGAG
CAGACTGCGCAGGGTTGGTGGCTCCAATAATAGTGCGGCCAGTCTTAACA
ACACCTTCACCCTCCCAAATCATAGCAACAACAGGACCAGAGGTAATGTA
CTCAATCAGAGAACCAAAGAAAGGCTTCGATGACAAATCCTCATAGTGCT
TCTCAGCAAAAGGGCGGTCCACAGTCATAAGCTTCAAACCCTTCAAGGAG
AAACCCTTCTTCTCAAATCTGATGATGATTTCACCAACAAGGCCTCTTTG
GACCCCATCGGGCTTGATCATGATGAAAGTTTGTTCCATTTCTT
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