DY258284

Overview
NameDY258284
Unique NameDY258284
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1315
Libraries
Library NameType
AbsDev1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: CIPKQ_ORYSJ (CBL-interacting protein kinase 26 OS=Oryza sativa subsp. japonica GN=CIPK26 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.814e-15
Identity = 61/203 (30.05%), Postives = 99/203 (48.77%), Query Frame = 3
Query:  204 LGRGGFGKVYKGR-LADGKLVAVKRLKEERTS--GGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYK-DTHITTAVHGTVGHIAPEYLS-TGRSSEKTDVFGYGIMLLELITGQRAF 797
            LG+G F KVY  R L  G+ VA+K + +E+ +  G  +Q + E+ I+ +  H N+L L     + ++   V  Y   G +  ++ + + S         + I+     G+ Y H      + HRD+K  N+LLDE     V DFGL+ L + K    +     GT  ++APE LS  G    K D++  G++L  L++G   F
Sbjct:   18 LGQGNFAKVYYARNLTSGQAVAIKMIDKEKVTRVGLMVQIKREISIMRLVKHPNILQLFEVMASKSKIYFVLEYAKGGELFKKISKGKFSEDVARRYFHQLIS-----GIDYCHSR---GVYHRDLKPENLLLDENESLKVSDFGLSALSESKRHDGLLHTTCGTPAYVAPEVLSRRGYDGAKADIWSCGVILFVLVSGYLPF 212          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: BCK1_YEAST (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae GN=BCK1 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.814e-15
Identity = 61/206 (29.61%), Postives = 103/206 (50.00%), Query Frame = 3
Query:  201 ILGRGGFGKVYKG-RLADGKLVAVKRLKEERTSGGELQFQTEVKIISMAV-------HRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHGTVGHIAPEYLSTGRS-SEKTDVFGYGIMLLELITGQR 791
            ++G+G FG VY    +  G+++AVK+++  + S       + V+ +   V       H N++   GF        L   Y+A GSV S +R         D P  K +     +GL+YLH      I+HRD+KA N+LLD++    + DFG+++      ++    + GTV  +APE + T +  S K D++  G ++LE+  G+R
Sbjct: 1180 MIGKGSFGAVYLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKDLDHLNIVQYLGFENKNNIYSLFLEYVAGGSVGSLIR----MYGRFDEPLIKHLTTQVLKGLAYLHSK---GILHRDMKADNLLLDQDGICKISDFGISRKSKDIYSNSDMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKR 1378          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: Y9865_DICDI (Probable serine/threonine-protein kinase DDB_G0280461 OS=Dictyostelium discoideum GN=DDB_G0280461 PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.982e-15
Identity = 61/215 (28.37%), Postives = 108/215 (50.23%), Query Frame = 3
Query:  156 LRELQVATDGFS-NKNILGRGGFGKVYKGRLADGKLVAVKRLKEERTSGGE--LQFQTEVKIISMAV---HRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT 782
            +++  +  D     +N   +G F KVYKG    G  V VK +K++     E  +  + E+ I+   +   H+N++   G     +   LV   +  G + + L + +  +P   W  R KIA   A G+ YLH     +I+HRD+K+ N+LL   +   + DFG AK +  ++  ++  + GT   ++PE +   + S   D++ +G++LLELIT
Sbjct:    3 IKDFNIECDNLKIEENEFSKGSFAKVYKGSYY-GNPVCVKVIKKDTLIDKESWVFLKREIGILKNLLNQGHKNIIRFIGIGEKDSLLFLVTELINGGDLGNILLDHKFHIP---WSLRVKIAKDIAEGMEYLHSK---QIMHRDLKSNNLLLGRNWTIKICDFGFAKEITIQNP-LSMTICGTDEFMSPEVILGIQYSYSADIYSFGMVLLELIT 209          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: FGFR4_RAT (Fibroblast growth factor receptor 4 OS=Rattus norvegicus GN=Fgfr4 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.982e-15
Identity = 66/216 (30.56%), Postives = 105/216 (48.61%), Query Frame = 3
Query:  204 LGRGGFGKVYKG--------RLADGKLVAVKRLKEERTSGGELQFQTEVKIISM-AVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQ------------SSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHG--TVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT 782
            LG G FG+V +         R      VAVK LK+  +        +E++++ +   H+N++ L G CT      ++  Y A G++   LR R+            SS  PL +P     A   ARG+ YL      K IHRD+ A N+L+ E+    + DFGLA+ + + D +  T+ +G   V  +APE L     + ++DV+ +GI+L E+ T
Sbjct:  471 LGEGCFGQVVRAEALGMDSSRPDQTSTVAVKMLKDNASDKDLADLISEMEMMKLIGRHKNIINLLGVCTQEGPLYVIVEYAAKGNLREFLRARRPPGPDLSPDGPRSSEGPLSFPALVSCAYQVARGMQYLESR---KCIHRDLAARNVLVTEDDVMKIADFGLARGVHHIDYYKKTS-NGRLPVKWMAPEALFDRVYTHQSDVWSFGILLWEIFT 682          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: EPHB4_HUMAN (Ephrin type-B receptor 4 OS=Homo sapiens GN=EPHB4 PE=1 SV=2)

HSP 1 Score: 82.8037 bits (203), Expect = 4.982e-15
Identity = 65/212 (30.66%), Postives = 111/212 (52.36%), Query Frame = 3
Query:  195 KNILGRGGFGKVYKGRL-ADGK---LVAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVAS--RLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMD--YKDTHITTAVHG--TVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT-GQRAF 797
            + ++G G FG+V +GRL A GK    VA+K LK   T     +F +E  I+    H N++ L G  T     +++  +M NG++ S  RL + Q ++  L    R     G A G+ YL E      +HRD+ A NIL++      V DFGL++ ++    D   T+++ G   +   APE ++  + +  +D + YGI++ E+++ G+R +
Sbjct:  618 EEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLR-----GIASGMRYLAE---MSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPY 821          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: EPA4A_XENLA (Ephrin type-A receptor 4-A OS=Xenopus laevis GN=epha4-A PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.982e-15
Identity = 61/208 (29.33%), Postives = 103/208 (49.52%), Query Frame = 3
Query:  195 KNILGRGGFGKVYKGRL-ADGK---LVAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLM--DYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT-GQRAF 797
            + ++G G FG+V  GRL   GK    VA+K LK   T      F +E  I+    H N++ L G  T     +++  YM NGS+ + LR+       +       I  G   G+ YL    D   +HRD+ A NIL++      V DFG+++++  D +  + T      +   APE ++  + +  +DV+ YGI++ E+++ G+R +
Sbjct:  623 EKVIGVGEFGEVCSGRLKVPGKREIYVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQ---LVGILRGIGSGMKYL---SDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPY 824          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: EPA4A_DANRE (Ephrin type-A receptor 4a (Fragment) OS=Danio rerio GN=epha4a PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.982e-15
Identity = 65/214 (30.37%), Postives = 103/214 (48.13%), Query Frame = 3
Query:  204 LGRGGFGKVYKGRLA-DGK---LVAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMD------YKDTHITTAVHGTVGHI-----APEYLSTGRSSEKTDVFGYGIMLLELIT-GQRAF 797
            +G G FG+V  GRL   GK    VA+K LK   T      F +E  I+    H N++ L G  T     +++  YM NGS+ + LR+       +       I  G A G+ YL    D   +HRD+ A NIL++      V DFG++++++      Y    IT       G I     APE ++  + +  +DV+ YGI++ E+++ G+R +
Sbjct:    1 IGIGEFGEVCSGRLKMPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIRLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQ---LVGILRGIASGMKYL---SDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEEDPDAAYTTREITGTYQSQGGKIPIRWTAPEAITYRKFTSASDVWSYGIVMWEVMSYGERPY 208          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: TSUA_DICDI (Probable serine/threonine-protein kinase tsuA OS=Dictyostelium discoideum GN=tsuA PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 66/227 (29.07%), Postives = 108/227 (47.58%), Query Frame = 3
Query:  204 LGRGGFGKVYKGRLA-DGKLVAVKRL-KEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELITGQRAF------DLAMLANDENVMLLDRVKGYP 860
            +G G FGKVYK R    G+LVA K + K+ +     L  + E+ I+    H N++          E  LV  Y A+G ++  + E ++    L     + I       L+YLH     K+IHRD+K  NIL+    +  V DFG AK +   ++ + T++ GT  ++APE +       K D++  GI+L +++ G   F      DL  +  + N+ +   +  YP
Sbjct:   11 IGEGSFGKVYKYRKKFTGQLVACKVISKKGKNEEDILSLRQEIDILKNLSHPNIIQFISCFENKNEFTLVTEY-ADGDLSQIISEEKT----LSVDLIQSICYQLVIALNYLHYK---KVIHRDIKPQNILITSGGQIKVCDFGFAKTIS-SNSILLTSLKGTPLYLAPEIIQEQPYDYKADLWSLGIILYQILVGSPPFSANSLADLVHMTLESNIEIPKELNKYP 228          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: ROS_CHICK (Proto-oncogene tyrosine-protein kinase ROS OS=Gallus gallus GN=ROS1 PE=2 SV=3)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 71/225 (31.56%), Postives = 109/225 (48.44%), Query Frame = 3
Query:  180 DGFSNKNILGRGGFGKVYKGR----LADGK---LVAVKRLKEERTSGGELQFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRL---RERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEE------FEAVVGDFGLAKLMDYKDTHITTAVHG--TVGHIAPEYLSTGRSSEKTDVFGYGIMLLELIT-GQRAF 797
            D  +   +LG G FG+VY+G     LADG     VAVK LK   T   + +F  E  ++S   H ++L L G C     + L+   M  G + S L   R+++   P L       I L   +G  YL +    + IHRD+ A N L+ E+          +GDFGLA+ + YK+ +      G   V  +APE L  G  +  +DV+ +G+++ E +T GQ+ +
Sbjct: 1959 DKLNLHKLLGSGAFGEVYEGTALDILADGSGESRVAVKTLKRGATDQEKSEFLKEAHLMSKFDHPHILKLLGVCLLNEPQYLILELMEGGDLLSYLRGARKQKFQSPLLTLTDLLDICLDICKGCVYLEK---MRFIHRDLAARNCLVSEKQYGSCSRVVKIGDFGLARDI-YKNDYYRKRGEGLINVRWMAPESLIDGVFTNHSDVWAFGVLVWETLTLGQQPY 2179          
BLAST of DY258284 vs. ExPASy Swiss-Prot
Match: PLK4_DROWI (Serine/threonine-protein kinase PLK4 OS=Drosophila willistoni GN=SAK PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 6.506e-15
Identity = 65/219 (29.68%), Postives = 103/219 (47.03%), Query Frame = 3
Query:  195 KNILGRGGFGKVYKGRLADG-KLVAVKRLKEERTSGGEL--QFQTEVKIISMAVHRNLLGLCGFCTTVTEKLLVYRYMANGSVASRLRERQSSLPPLDWPTRKKIALGSARGLSYLHEHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKD-THITTAVHGTVGHIAPEYLSTGRSSEKTDVFGYGIMLLELITGQRAFDLAMLANDENVMLL 839
            +++LG+GGF  VYK R     + VA+K + ++   G  L  + + EV+I S   H ++L L  F        LV     NG +   + ++ S   P        I      GL YLH H    I+HRD+  +N+LL ++    + DFGLA  +   D  H+T    GT   I+PE +S        DV+  G +L  L+ G+  FD   + +  N +++
Sbjct:   17 QHLLGKGGFASVYKARCRRTYQDVAIKMIDKKLIHGTGLSSRVRQEVEIHSRLKHPSVLQLYTFFQDANYVYLVLELAHNGELHRYMNQQMSH--PFTEADAATILQQVVAGLLYLHSH---NIMHRDISLSNLLLSKDMHVKIADFGLATQLKRPDEKHMTMC--GTPNFISPEVVSRLSHGLAADVWSVGCLLYTLVVGRPPFDTDAVQSTLNKVVM 228          
The following BLAST results are available for this feature:
BLAST of DY258284 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CIPKQ_ORYSJ3.814e-1530.05CBL-interacting protein kinase 26 OS=Oryza sativa ... [more]
BCK1_YEAST3.814e-1529.61Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS... [more]
Y9865_DICDI4.982e-1528.37Probable serine/threonine-protein kinase DDB_G0280... [more]
FGFR4_RAT4.982e-1530.56Fibroblast growth factor receptor 4 OS=Rattus norv... [more]
EPHB4_HUMAN4.982e-1530.66Ephrin type-B receptor 4 OS=Homo sapiens GN=EPHB4 ... [more]
EPA4A_XENLA4.982e-1529.33Ephrin type-A receptor 4-A OS=Xenopus laevis GN=ep... [more]
EPA4A_DANRE4.982e-1530.37Ephrin type-A receptor 4a (Fragment) OS=Danio reri... [more]
TSUA_DICDI6.506e-1529.07Probable serine/threonine-protein kinase tsuA OS=D... [more]
ROS_CHICK6.506e-1531.56Proto-oncogene tyrosine-protein kinase ROS OS=Gall... [more]
PLK4_DROWI6.506e-1529.68Serine/threonine-protein kinase PLK4 OS=Drosophila... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI1AA05FM2 AbsDev1 Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY258284 ID=DY258284; Name=DY258284; organism=Citrus clementina; type=EST; length=1315bp
TTCCTGTGGGAGTTGCTCTTGGCGCTGCTTTACTTTTTGCTGTACCAGTT
ATTGGGTTTGCATATTGGCGGCGAACAAGACCACACGAATTTTTCTTTGA
CGTACCTGCTGAAGATGATTCAGAACTCCAGTTGGGACAGTTGAAAAGGT
TTTCTTTGCGAGAACTACAAGTTGCTACAGATGGTTTTAGCAACAAGAAC
ATCCTGGGAAGAGGTGGATTTGGCAAGGTGTATAAGGGACGCTTGGCTGA
TGGCAAATTGGTGGCAGTCAAAAGACTTAAGGAAGAGAGGACATCTGGAG
GGGAGTTGCAGTTTCAAACAGAAGTGAAGATAATCAGCATGGCTGTGCAT
CGGAATCTTCTGGGATTATGTGGGTTTTGCACGACTGTCACTGAGAAACT
GCTTGTTTATCGATACATGGCCAATGGAAGTGTTGCATCACGCCTAAGAG
AGCGGCAATCATCACTCCCGCCTCTTGACTGGCCAACTAGGAAGAAAATT
GCTTTAGGTTCAGCGAGGGGCCTTTCATATTTGCACGAGCATTGTGACCC
AAAGATCATTCATCGAGATGTCAAAGCTGCAAACATATTGTTGGATGAAG
AGTTTGAGGCTGTCGTTGGTGACTTTGGTTTAGCTAAACTAATGGACTAC
AAGGACACCCACATAACCACTGCTGTGCATGGCACCGTTGGGCATATAGC
CCCAGAGTATCTCTCCACAGGGAGGTCATCAGAGAAAACGGATGTTTTTG
GTTACGGTATCATGCTGTTAGAGCTAATTACCGGACAAAGGGCATTTGAT
CTTGCTATGCTTGCAAACGATGAAAATGTTATGCTCCTTGATAGAGTTAA
AGGATACCCTANGAGAGAAGAATTTTGATGTACTTGTTGATAAAGACCTG
CCGACTGATTATGAATGAAACTGAAGTGGGAAGAACTCCTCCCAAGTGGC
CCTCCCTTGCCCCCCAACCCCCCCCATCCCACGGGCCCAAAAAAGTCGAA
ATTTGCCAAAATGCTTGAAAGGGCATGGGCTGGGCAAAAAAAAAGGGGGC
AGGGGGGCCAACGGCCGGAGGGTATCCCCCCCCCAAGAAATCTTCATTTG
CCCCACCGGGCAAAAAATTTAAAAAAAAAAAAATTTACCCTCCCCCCCCA
CCCCTTTTTCAAAAATTGCTTTTTTCCCGCGGGCCGCCAAAAAAGAAAAA
GATTTTTTTCCTGTTGGGGCAAACAAAAAGAAAAAACCACCACAACCCCC
CCCTCTTCTCCCCCCTCCCCCCCCCTATTAATTAAGGGAGGGGGGGGTTT
GATTAAACCCCCCCC
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