DY258444

Overview
NameDY258444
Unique NameDY258444
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1329
Libraries
Library NameType
AbsDev1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
DY258444_ssr218 microsatellite DY258444_ssr218:218..229. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY258444 vs. ExPASy Swiss-Prot
Match: UBC1_MAGGR (Ubiquitin-conjugating enzyme E2-16 kDa OS=Magnaporthe grisea GN=UBC1 PE=3 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 5.236e-12
Identity = 33/75 (44.00%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  206 VFHPNNNNNDRLCLDIAKERCIPALTTSKALLAICSLITXXXXXXXXXXXXXHMHNTDRAPYDSTARSWTHTSAM 430
            ++HPN N+N  +CLDI +++  PALT SK LL+ICS++T             H++ T RA Y+STAR WT   A+
Sbjct:   73 IYHPNINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDDPLVPEIAHVYKTARAQYESTAREWTRKYAI 147
[Close]
BLAST of DY258444 vs. ExPASy Swiss-Prot
Match: UBC4_CANAL (Ubiquitin-conjugating enzyme E2 4 OS=Candida albicans GN=UBC4 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 8.932e-12
Identity = 33/81 (40.74%), Postives = 49/81 (60.49%), Query Frame = 2 
Query:  188 VILSYWVFHPNNNNNDRLCLDIAKERCIPALTTSKALLAICSLITXXXXXXXXXXXXXHMHNTDRAPYDSTARSWTHTSAM 430
            + L+  ++HPN N+N  +CLDI K++  PALT SK LL+ICSL+T             H++  DR  Y++TA+ WT   A+
Sbjct:   67 IALTTKIYHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTDANPDDPLVPEIAHIYKQDRKKYEATAKEWTKKYAV 147
[Close]
BLAST of DY258444 vs. ExPASy Swiss-Prot
Match: UBC4_SCHPO (Ubiquitin-conjugating enzyme E2 4 OS=Schizosaccharomyces pombe GN=ubc4 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.524e-11
Identity = 32/75 (42.67%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  206 VFHPNNNNNDRLCLDIAKERCIPALTTSKALLAICSLITXXXXXXXXXXXXXHMHNTDRAPYDSTARSWTHTSAM 430
            ++HPN N+N  +CLDI +++  PALT SK LL+ICSL+T             H++ TDR+ Y+ +AR WT   A+
Sbjct:   73 IYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYELSAREWTRKYAI 147
[Close]
BLAST of DY258444 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.990e-11
Identity = 33/75 (44.00%), Postives = 46/75 (61.33%), Query Frame = 2 
Query:  206 VFHPNNNNNDRLCLDIAKERCIPALTTSKALLAICSLITXXXXXXXXXXXXXHMHNTDRAPYDSTARSWTHTSAM 430
            V+HPN ++   +CLDI KE+  PA TTSK LL+ICSL+T             H++  D++ Y+STA+ WT   AM
Sbjct:   74 VYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAM 148
[Close]
BLAST of DY258444 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 5.790e-11
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 2 
Query:  206 VFHPNNNNNDRLCLDIAKERCIPALTTSKALLAICSLITXXXXXXXXXXXXXHMHNTDRAPYDSTARSWTHTSAM 430
            ++HPN N++  +CLDI K++  PALT SK LL+ICSL+T              ++ TD+A Y++TA+ WT   A+
Sbjct:   74 IYHPNINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKEWTKKYAV 148
The following BLAST results are available for this feature:
BLAST of DY258444 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 15
Match NameE-valueIdentityDescription
UBC1_MAGGR5.236e-1244.00Ubiquitin-conjugating enzyme E2-16 kDa OS=Magnapor... [more]
UBC4_CANAL8.932e-1240.74Ubiquitin-conjugating enzyme E2 4 OS=Candida albic... [more]
UBC4_SCHPO1.524e-1142.67Ubiquitin-conjugating enzyme E2 4 OS=Schizosacchar... [more]
UBC12_ARATH1.990e-1144.00Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
UBC5_YEAST5.790e-1141.33Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI1DC03FM2 AbsDev1 Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY258444 ID=DY258444; Name=DY258444; organism=Citrus clementina; type=EST; length=1329bp
TCTTTTTCGGGTTCTTAATTTTATATCCTTAATTTGTGTGTACTTATTTT
AAGAGTTATGCTATGTATTTTGGTCAAGTTACCGGAAACAATGTGAGTTG
ATTTAACTTGTCCCCTGAATGGTTGCTTTCGGTATTTATCATGTGGCCTG
TATGGTGGCAAAAGAAAAGTCTCCTTTTTACGACATAGTTATCCTTTCCT
ATTGGGTTTTCCACCCAAACAACAACAACAATGATAGACTCTGTCTTGAC
ATTGCCAAGGAGCGGTGTATCCCCGCTTTGACCACATCCAAGGCACTTCT
GGCAATTTGCTCCCTCATTACTGATCCAAACCCAATTGACCCTCCTGCGC
CTGACATCGCTCACATGCACAATACTGACAGAGCCCCATATGACAGTACT
GCTCGATCCTGGACCCACACCTCTGCCATGGACTTAGATGGGCTAGGCTG
CCCCCCCCCCACAACATTACATGGCTCGCGGCTCTATTCCCAAATACTAT
AAATCCCTTTGTTTCCTATATTAAAATACTATATTTGGCTTTGTTCCCCA
AATCGCTAAAAAGTCTTCCGTCGCGTTACTCCCCCCTTTACCCTTGCCCC
TCAACACTCACTTTCCTCCCACCCGCCAAAACAAAACACAAAACATAACC
CAAAACTCCCCCCTTCTCCCCCCCTTCTCTGTCAAATCAAAACACCCCTG
AAAATTCATCTTGTCCCACCAACCCGGCCACCCTTCCCCTATCTCTACTC
CTTCTTCCCTACCCCCCGCATACCCCTTTACATCACCGTACTATCTGCCC
CCACCCTTTTTCTGCAGACCCACCCCGCCATCCTCCCATTCAACCCGATC
CTAATTCCCCTCAAATACACTACAAAATACCCGACCCACCCAAACCTTTC
CCCCATCCCCTTCTACCCTCCCACTTCCTCACATCCACATCACACACGCA
CCTTTCTCTTATCATCCCCCAACTTAATACTCCCCCCCCGCACCACTACC
AAGCACCACTCCTCACCAAACGCCGTCACGCTCCCCTTTTGTCACCGCCC
GCACCCACTCCTCAACCACCCCACTCCACACCCCCACCCTACCCCCCCCC
CTCTCATTCCACCTCCTCCATCAACCGACACTCACCCCCTCCCGCTCCTC
CCACTCATCCCCGTCCCCACGACACGACACACCTGCTCACCATCATTCCT
CCCCTCCTCCCCCCCACCTCACCCACCACCCTCCCCGCTCCCTTCCCTAC
CCTCTCCCTCTCCCCCCTTTTCCCTCTTCACTCCTCCCCTCACCCAATTC
CATCTCCTACATTCCTCCTATCTCTTCAC
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