DY258515 | Citrus Genome Database

Overview


Name	DY258515
Unique Name	DY258515
Type	EST
Organism	Citrus clementina (Clementine)
Sequence length	1287

Libraries


Library Name	Type
AbsDev1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
DY258515_ssr820	microsatellite	DY258515_ssr820:820..834.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: ACSF2_MACFA (Acyl-CoA synthetase family member 2, mitochondrial OS=Macaca fascicularis GN=ACSF2 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 7.045e-20
Identity = 52/199 (26.13%), Postives = 91/199 (45.73%), Query Frame = 3

Query:   93 DPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVLP--LFH-IYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPXTG 680
            DP+ + F+SGTTG PKG  L+H +++ +    + GE   L+    + +  +LP  L+H + S+   ++C +     +L    F     LE I R R S               P  + YD+S++R  +   +P   EL  A+ +++    L   YG TE  PV      F +     K+ S G ++ + E ++++   G
Sbjct:  259 DPINIQFTSGTTGSPKGATLSHYNIVNN--SSILGERLKLHEKTPEQLRMILPSPLYHCLGSVGGTMMCLMYGATLILASPVFNGKKALEAISRERGSFLYGTPTMFVDILNQPDFSSYDISTMRGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPV--TFANFPEDTVEQKAESVGRIMPHTEARIMNMEAG 453

HSP 2 Score: 46.595 bits (109), Expect = 7.045e-20
Identity = 18/40 (45.00%), Postives = 26/40 (65.00%), Query Frame = 2

Query:  695 NQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            N PGE+CIRG  +M GY  +P+ T   +D + W  TGD++
Sbjct:  459 NTPGELCIRGYCVMLGYWGEPQKTGEAVDQDKWYRTGDIA 498

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: LCFA_YERPE (Long-chain-fatty-acid--CoA ligase OS=Yersinia pestis GN=fadD PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.194e-19
Identity = 54/204 (26.47%), Postives = 101/204 (49.51%), Query Frame = 3

Query:   81 IKPD----DPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVLPLFHIYSL--NSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTK--SGSCGTVVRNAELKVID 668
            +KPD    D   L ++ GTTG+ KG +LTH+++ +++ Q      P L     D+V+  LPL+HI++L  N +L   L  G  +L+    +I  +++ + R+  +              N      D S++R+ + G  P+ K + +   +   + +L +GYG+TE  P+++        P+  K  SGS G  V + ++++ D
Sbjct:  199 VKPDVINTDTAFLQYTGGTTGVAKGAILTHRNMQSNLEQAKAAYAP-LLQPGRDLVVTALPLYHIFALTVNCLLFIEL-GGRSLLITNPRDIPGMVKELSRYPFTAITGVNTLFNALLNNEEFTHLDFSTLRLSVGGGMPVQKAVAEKWETLTGKHLL-EGYGLTECSPLVT------GNPYDLKHYSGSIGLPVPSTDVRLRD 393

HSP 2 Score: 45.0542 bits (105), Expect = 1.194e-19
Identity = 19/39 (48.72%), Postives = 28/39 (71.79%), Query Frame = 2

Query:  698 QPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            +PGE+ +RGPQ+M GY   P+AT   +  +GWL TGD++
Sbjct:  403 KPGELWVRGPQVMLGYWQRPDATDDVLK-DGWLATGDIA 440

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: ACSF2_BOVIN (Acyl-CoA synthetase family member 2, mitochondrial OS=Bos taurus GN=ACSF2 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 5.764e-19
Identity = 56/199 (28.14%), Postives = 90/199 (45.23%), Query Frame = 3

Query:   93 DPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVL--PLFHIYSLNSVLLCSLRAGAGVLLMQK-FEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPXTG 680
            DP+ + F+SGTTG PKG  L+H +++ +    + G+   L+    +    VL  PL+H        + SL  G  ++L    FE    LE I R R                 P  + YD+S++R  +   +P   EL  A+ +++    L   YG TE  PV  M   F +     K+ S G V+ + E ++++  TG
Sbjct:  256 DPINIQFTSGTTGSPKGATLSHYNIVNNA--NMIGQRLRLHQKTPEESRVVLPSPLYHCLGSVGGTMVSLMHGVTLILCSPVFEGKKTLEAISRERGCFLYGTPTMFVDVLNQPDFSSYDISTMRGGVIAGSPAPPELIRAIINKLNMKELVVAYGTTENSPVTFM--NFTEDTVEQKAESVGRVMPHTEAQIVNTETG 450

HSP 2 Score: 43.8986 bits (102), Expect = 5.764e-19
Identity = 18/40 (45.00%), Postives = 25/40 (62.50%), Query Frame = 2

Query:  695 NQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            N PGE+CIRG  +M GY  +P+ T   I  + W  TGD++
Sbjct:  456 NTPGELCIRGYCVMLGYWGEPQKTEEAIGQDKWYRTGDIA 495

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: LCFA_SALTY (Long-chain-fatty-acid--CoA ligase OS=Salmonella typhimurium GN=fadD PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 5.774e-19
Identity = 54/202 (26.73%), Postives = 96/202 (47.52%), Query Frame = 3

Query:   69 PEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVLPLFHIYSL--NSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVID 668
            PEV    +D   L ++ GTTG+ KG +LTH++++ ++ Q      P L+    ++V+  LPL+HI++L  N +L   L  G  +L+    +I  L++ + ++  +              N      D SS+ +   G  P+   + +       Q +L +GYG+TE  P++S+            SGS G  V + E K++D
Sbjct:  201 PEVVA--EDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVKATYGPLLH-PGKELVVTALPLYHIFALTMNCLLFIEL-GGQNLLITNPRDIPGLVKELAKYPFTAMTGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQNVVAERWVKLTGQYLL-EGYGLTECAPLVSV----NPHDIDYHSGSIGLPVPSTEAKLVD 393

HSP 2 Score: 45.4394 bits (106), Expect = 5.774e-19
Identity = 19/39 (48.72%), Postives = 28/39 (71.79%), Query Frame = 2

Query:  698 QPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            + GE+C++GPQ+M GY   P+AT   I  +GWL TGD++
Sbjct:  403 EAGELCVKGPQVMLGYWQRPDATDEIIK-DGWLHTGDIA 440

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: LCFA_SALTI (Long-chain-fatty-acid--CoA ligase OS=Salmonella typhi GN=fadD PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 5.774e-19
Identity = 54/202 (26.73%), Postives = 96/202 (47.52%), Query Frame = 3

Query:   69 PEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVLPLFHIYSL--NSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVID 668
            PEV    +D   L ++ GTTG+ KG +LTH++++ ++ Q      P L+    ++V+  LPL+HI++L  N +L   L  G  +L+    +I  L++ + ++  +              N      D SS+ +   G  P+   + +       Q +L +GYG+TE  P++S+            SGS G  V + E K++D
Sbjct:  201 PEVVA--EDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVKATYGPLLH-PGKELVVTALPLYHIFALTMNCLLFIEL-GGQNLLITNPRDIPGLVKELAKYPFTAMTGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQNVVAERWVKLTGQYLL-EGYGLTECAPLVSV----NPHDIDYHSGSIGLPVPSTEAKLVD 393

HSP 2 Score: 45.4394 bits (106), Expect = 5.774e-19
Identity = 19/39 (48.72%), Postives = 28/39 (71.79%), Query Frame = 2

Query:  698 QPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            + GE+C++GPQ+M GY   P+AT   I  +GWL TGD++
Sbjct:  403 EAGELCVKGPQVMLGYWQRPDATDEIIK-DGWLHTGDIA 440

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: ACSF2_RAT (Acyl-CoA synthetase family member 2, mitochondrial OS=Rattus norvegicus GN=Acsf2 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 9.746e-19
Identity = 57/201 (28.36%), Postives = 92/201 (45.77%), Query Frame = 3

Query:   93 DPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVL--PLFHIYSLNSVLLCSLRAGAGVLLMQ-KFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIR--VVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPXTG 680
            DP+ + F+SGTTG PKG  L+H +++ +    + G+   +     + +  VL  PL+H        + S+  GA +LL    F     LE I R + +               P  + YD ++IR  V+    AP   EL  A+ S++    L   YG TE  PV  M   F +     K+GS G ++ + E ++++  TG
Sbjct:  256 DPINIQFTSGTTGNPKGATLSHHNIVNN--SNLIGQRLKMPAKTAEELRMVLPCPLYHCLGSVGGTMVSVVHGATLLLSSPSFNGKKALEAISREKGTLLYGTPTMFVDILNQPDFSSYDFTTIRGGVIAGSLAP--PELIRAIISKMNMKELVVVYGTTENSPVTFM--NFPEDTLEQKAGSVGRIMPHTEAQIVNMETG 450

HSP 2 Score: 46.595 bits (109), Expect = 9.746e-19
Identity = 18/40 (45.00%), Postives = 27/40 (67.50%), Query Frame = 2

Query:  695 NQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            N PGE+CIRG  +M+GY  +P+ T  T+  + W  TGD++
Sbjct:  456 NMPGELCIRGYCVMQGYWGEPQKTFETVGQDRWYRTGDIA 495

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: ACSF2_MOUSE (Acyl-CoA synthetase family member 2, mitochondrial OS=Mus musculus GN=Acsf2 PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.267e-18
Identity = 55/199 (27.64%), Postives = 91/199 (45.73%), Query Frame = 3

Query:   93 DPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVL--PLFHIYSLNSVLLCSLRAGAGVLLMQ-KFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPXTG 680
            DP+ + F+SGTTG PKG  L+H +++ +    + G+   +     + +  VL  PL+H        + S+  GA +LL    F     LE I R + +               P  + YD +SIR  +   +P   EL  A+ +++    L   YG TE  PV  M   F +     K+GS G ++ + E ++++  TG
Sbjct:  256 DPINIQFTSGTTGNPKGATLSHHNIVNN--SMLIGQRLKMPTKTAEELRLVLPSPLYHCLGSVGGTMVSMMHGATLLLSSPSFNGKKALEAISREKGTLLYGTPTMFVDILNQPDFSSYDFTSIRGGVIAGSPAPPELIRAIINKMNMKELVVVYGTTENSPVTFM--NFPEDTLEQKAGSVGRIMPHTEAQIVNVETG 450

HSP 2 Score: 41.9726 bits (97), Expect = 1.267e-18
Identity = 18/44 (40.91%), Postives = 28/44 (63.64%), Query Frame = 2

Query:  683 LPSHNQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            L + N PGE+ IRG  +M+GY  +P+ T  T+  + W  TGD++
Sbjct:  452 LTNLNVPGELYIRGYCVMQGYWGEPQKTFETVGQDKWYRTGDIA 495

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: ACSF2_HUMAN (Acyl-CoA synthetase family member 2, mitochondrial OS=Homo sapiens GN=ACSF2 PE=1 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 4.709e-18
Identity = 52/212 (24.53%), Postives = 95/212 (44.81%), Query Frame = 3

Query:   54 DEDQIPEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVL--PLFH-IYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPXTG 680
            D+ Q  +  +   DP+ + F+SGTTG PKG  L+H +++ +    + GE   L+    + +  +L  PL+H + S+   ++C +     +L    F     LE I R R +               P  + YD+S++   +   +P   EL  A+ +++    L   YG TE  PV      F +     K+ S G ++ + E ++++   G
Sbjct:  243 DQLQYNQQFLSCHDPINIQFTSGTTGSPKGATLSHYNIVNN--SNILGERLKLHEKTPEQLRMILPNPLYHCLGSVAGTMMCLMYGATLILASPIFNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPV--TFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAG 450

HSP 2 Score: 45.8246 bits (107), Expect = 4.709e-18
Identity = 18/40 (45.00%), Postives = 26/40 (65.00%), Query Frame = 2

Query:  695 NQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            N PGE+CIRG  +M GY  +P+ T   +D + W  TGD++
Sbjct:  456 NTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVA 495

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: ACSF2_PONAB (Acyl-CoA synthetase family member 2, mitochondrial OS=Pongo abelii GN=ACSF2 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 7.960e-18
Identity = 52/212 (24.53%), Postives = 94/212 (44.34%), Query Frame = 3

Query:   54 DEDQIPEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVL--PLFH-IYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPXTG 680
            D+ Q  +  +   DP+ + F+SGTTG PKG  L+H +++ +    + GE   L+    + +  +L  PL+H + S+   ++C +     +L    F     LE I R R S               P  + YD+S++   +   +P   EL  A+ +++    L   YG  E  PV      F +     K+ S G ++ + E ++++   G
Sbjct:  243 DQLQYNQQFLSCHDPINIQFTSGTTGSPKGATLSHYNIVNN--SNMLGERLKLHEKTPEQLRMILPSPLYHCLGSVGGTMMCLMYGATLILASPVFNGKKALEAISRERGSFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELIRAIINKINMKDLVVAYGTPENSPV--TFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAG 450

HSP 2 Score: 45.8246 bits (107), Expect = 7.960e-18
Identity = 18/40 (45.00%), Postives = 26/40 (65.00%), Query Frame = 2

Query:  695 NQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDLS 814
            N PGE+CIRG  +M GY  +P+ T   +D + W  TGD++
Sbjct:  456 NTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYRTGDVA 495

[Close]

BLAST of DY258515 vs. ExPASy Swiss-Prot
Match: MENE_BACA2 (2-succinylbenzoate--CoA ligase OS=Bacillus amyloliquefaciens (strain FZB42) GN=menE PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.096e-17
Identity = 64/209 (30.62%), Postives = 97/209 (46.41%), Query Frame = 3

Query:   45 SEADEDQIPEVAIKPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRHRVSXXXXXXXXXXXXXKNPMVADYDL--SSIRVVLSGAAPLGKE-LEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKV 662
            +E   D  PE  +  DD   L ++SGTTG PKGV  T  +  +S          NL +T  D  L  LPLFHI  L S L  S+  G  V+L QKF +  +L+ I  H+V+              + ++A+ +    S+R +L G  P     LE+  R + P   + Q YG+TE     S  +  + +    K GS G  + + E+++
Sbjct:  130 AEEAGDIEPEAYVTLDDTATLMYTSGTTGRPKGVQQTFGNHYSSAVSSA----LNLGVTERDRWLIALPLFHISGL-SALFKSVIYGMPVVLHQKFSVTDVLDSISSHQVTIISAVQTML-----SGLLAETEQCPESLRCILLGGGPAPLPLLEECRRKQFP---VFQSYGLTE---TCSQIVTLSPEFSMDKLGSAGKPLFSCEIRI 322

HSP 2 Score: 39.2762 bits (90), Expect = 1.096e-17
Identity = 20/46 (43.48%), Postives = 26/46 (56.52%), Query Frame = 2

Query:  674 NWCLPSHNQPGEICIRGPQIMKGYLNDPEATAATIDVEGWLLTGDL 811
            N C P  +  GEI ++GP +MKGY +  +A  A     GW  TGDL
Sbjct:  327 NPCAPFEH--GEITVKGPNVMKGYYHRDDANQAAFH-NGWFKTGDL 369

HSP 3 Score: 24.6386 bits (52), Expect = 1.096e-17
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  895 PAEIEAPLLKPPSIAKA 945
            PAE+EA LL  P++A+A
Sbjct:  397 PAEVEAALLAHPAVAEA 413

The following BLAST results are available for this feature:

BLAST of DY258515 vs. ExPASy Swiss-Prot

BLAST of DY258515 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 148


Match Name	E-value	Identity	Description
ACSF2_MACFA	7.045e-20	26.13	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
LCFA_YERPE	1.194e-19	26.47	Long-chain-fatty-acid--CoA ligase OS=Yersinia pest...	[more]
ACSF2_BOVIN	5.764e-19	28.14	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
LCFA_SALTY	5.774e-19	26.73	Long-chain-fatty-acid--CoA ligase OS=Salmonella ty...	[more]
LCFA_SALTI	5.774e-19	26.73	Long-chain-fatty-acid--CoA ligase OS=Salmonella ty...	[more]
ACSF2_RAT	9.746e-19	28.36	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
ACSF2_MOUSE	1.267e-18	27.64	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
ACSF2_HUMAN	4.709e-18	24.53	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
ACSF2_PONAB	7.960e-18	24.53	Acyl-CoA synthetase family member 2, mitochondrial...	[more]
MENE_BACA2	1.096e-17	30.62	2-succinylbenzoate--CoA ligase OS=Bacillus amyloli...	[more]

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Properties


Property Name	Value
Genbank description	KN0AAI2AF11FM1 AbsDev1 Citrus clementina cDNA 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DY258515 ID=DY258515; Name=DY258515; organism=Citrus clementina; type=EST; length=1287bp
CGATCGATGCCCCTCCGGAGAATTGCCTGCATTTCACAGTGTTATCGGAA
GCAGATGAGGATCAAATCCCAGAAGTTGCCATCAAGCCCGACGATCCTGT
GGCGTTGCCTTTCTCTTCGGGCACCACGGGATTGCCTAAAGGAGTGGTGC
TGACGCACAAGAGCTTGATCACCAGCGTGGCGCAGCAAGTGGATGGAGAG
AACCCGAATCTTTACTTGACAAACGGAGACGTTGTGCTGTGCGTTTTACC
TTTGTTTCACATTTATTCGTTGAACAGCGTGCTTCTGTGCTCGTTGAGAG
CTGGGGCTGGGGTTCTGTTGATGCAGAAGTTTGAGATAGGGGCGCTGCTG
GAGCTGATACAGCGGCACAGGGTGTCGGTGGCGGCTGTGGTGCCGCCCTT
GGTGCTGGCGCTGGCCAAGAACCCGATGGTGGCGGATTATGACCTGAGCT
CGATCAGGGTGGTGCTTTCCGGGGCGGCGCCGCTGGGGAAGGAGCTTGAA
GATGCTCTCCGAAGTAGAGTCCCTCAAGCAATCTTGGGTCAGGGATATGG
AATGACAGAGGCAGGGCCAGTGCTATCAATGTGCCTAGGATTTGCAAAGC
AGCCATTTCCTACAAAGTCAGGATCATGTGGCACCGTTGTTAGAAATGCT
GAGCTCAAAGTTATTGACCCTGNAACTGGTGCCTCCCTTCCCACAATCAA
CCTGGCGAAATTTGCATCCGCGGCCCTCAAATTATGAAAGGTTACTTGAA
TGATCCTGAGGCAACTGCGGCAACTATAGACGTTGAGGGTTGGCTTCTTA
CTGGTGACCTAAGCTTTGTTGATGATGATGATGAGGTTTTCATAGTCGAT
AAGGTCAAGGGAATCATCAAATTCAAAGGGATCCAGGGTGCCGGCCGGCG
GAAATTGAAGCCCCCCTTTTAAAGCCACCATCCATTGCAAAAGCAGCTGG
GGTTCCCCCAAAAAAATGAAGTGGCAGGAAAAGTTCCGGTTGCTTTTGTA
TTTCCAACCATTGGGGTTTAACTTTACCGAGGAACCCATTTAAAAAATAC
AAACAAAAACGGGGGGGGGTTTAACAAAAACGGCACAAAATTTTTTTTTG
TTCCTTGCGCATCCCAAATTCCCTTTCCGGGAAAAATTTTGGGAAAAAAA
CCCCAAAAACCAAGACGCCCCCCTCCCCACGGCCCCCTTAAAATTATTTC
CTTTTCTTTTTATTATTACTCTTTTTGTTTTTTCTGTTCCGCCCCCGCTG
TTGGTGTGTGGGGGGCGATTTCCCACAAAAACCCCAC