DY258903

Overview
NameDY258903
Unique NameDY258903
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length947
Libraries
Library NameType
AbsDev1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX4_BOVIN (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Bos taurus GN=GPX4 PE=2 SV=2)

HSP 1 Score: 113.235 bits (282), Expect = 1.582e-27
Identity = 54/111 (48.65%), Postives = 77/111 (69.37%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F+ KDIDG+ V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P++  +K
Sbjct:   39 RSMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNAEIKEFA-AGYNVKFDLFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 31.9574 bits (71), Expect = 1.582e-27
Identity = 15/37 (40.54%), Postives = 20/37 (54.05%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFLSGQKWKSHRRESPPYLPL 726
            P   G+LG+ +KWNF KFL  +     +R  P   PL
Sbjct:  151 PKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX4_PIG (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Sus scrofa GN=GPX4 PE=1 SV=3)

HSP 1 Score: 109.383 bits (272), Expect = 2.262e-27
Identity = 53/111 (47.75%), Postives = 76/111 (68.47%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGS+ EIKEFA   +  +F +F K+ VNG +  P++  +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGYVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSDAEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 30.4166 bits (67), Expect = 2.262e-27
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFL 672
            P   G+LG+ +KWNF KFL
Sbjct:  151 PKGRGMLGNAIKWNFTKFL 169

HSP 3 Score: 25.0238 bits (53), Expect = 2.262e-27
Identity = 11/23 (47.83%), Postives = 14/23 (60.87%), Query Frame = 2 
Query:  674 VDKNGKVIEENPPPTSPFQIEKD 742
            +DKNG V++   P   P  IEKD
Sbjct:  170 IDKNGCVVKRYGPMEEPQVIEKD 192
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX41_MOUSE (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Mus musculus GN=Gpx4 PE=1 SV=4)

HSP 1 Score: 109.383 bits (272), Expect = 2.262e-27
Identity = 53/111 (47.75%), Postives = 76/111 (68.47%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F ++ K+ VNG +  P++  +K
Sbjct:   39 RSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYNVKFDMYSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 30.4166 bits (67), Expect = 2.262e-27
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFL 672
            P   G+LG+ +KWNF KFL
Sbjct:  151 PKGRGMLGNAIKWNFTKFL 169

HSP 3 Score: 25.0238 bits (53), Expect = 2.262e-27
Identity = 11/23 (47.83%), Postives = 14/23 (60.87%), Query Frame = 2 
Query:  674 VDKNGKVIEENPPPTSPFQIEKD 742
            +DKNG V++   P   P  IEKD
Sbjct:  170 IDKNGCVVKRYGPMEEPQVIEKD 192
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPO_LACLA (Glutathione peroxidase OS=Lactococcus lactis subsp. lactis GN=gpo PE=3 SV=2)

HSP 1 Score: 117.087 bits (292), Expect = 2.705e-27
Identity = 55/115 (47.83%), Postives = 72/115 (62.61%), Query Frame = 3 
Query:  285 SLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLKSKCWG 629
            + YDF+   ++G+ V +S FKGKV+++VN AS+CG TP  +  L  LYE YK QG EIL FPCNQF  Q+ G N EI EF    +   FP+F K+ VNG    P+Y  LK +  G
Sbjct:    2 NFYDFSAFKMNGETVSMSDFKGKVVIVVNTASKCGFTPQ-FEGLEKLYENYKDQGLEILGFPCNQFVNQDAGENSEINEFCQLNYGVTFPMFQKIKVNGKEAHPLYQFLKKEAKG 115

HSP 2 Score: 27.335 bits (59), Expect = 2.705e-27
Identity = 11/26 (42.31%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:  674 VDKNGKVIEENPPPTSPFQIEKDNQK 751
            +D+ G VIE   P T P ++E++ QK
Sbjct:  130 IDREGNVIERFAPKTEPKEMEEEIQK 155
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX4_PONPY (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Pongo pygmaeus GN=GPX4 PE=2 SV=2)

HSP 1 Score: 110.923 bits (276), Expect = 3.490e-27
Identity = 54/111 (48.65%), Postives = 76/111 (68.47%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P++  +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 33.113 bits (74), Expect = 3.490e-27
Identity = 16/37 (43.24%), Postives = 20/37 (54.05%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFLSGQKWKSHRRESPPYLPL 726
            P   GILG+ +KWNF KFL  +     +R  P   PL
Sbjct:  151 PKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX4_HUMAN (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Homo sapiens GN=GPX4 PE=1 SV=3)

HSP 1 Score: 110.923 bits (276), Expect = 3.490e-27
Identity = 54/111 (48.65%), Postives = 76/111 (68.47%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +  +F +F K+ VNG +  P++  +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 33.113 bits (74), Expect = 3.490e-27
Identity = 16/37 (43.24%), Postives = 20/37 (54.05%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFLSGQKWKSHRRESPPYLPL 726
            P   GILG+ +KWNF KFL  +     +R  P   PL
Sbjct:  151 PKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX3_YEAST (Peroxiredoxin HYR1 OS=Saccharomyces cerevisiae GN=HYR1 PE=1 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 3.589e-27
Identity = 71/162 (43.83%), Postives = 86/162 (53.09%), Query Frame = 3 
Query:  291 YDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLKSKCWGNFRRPCQVEL*EVLKWT-------KMEKSSKRIPPLPPPSKLRRTTRNL 755
            Y     D  G+  P  + KGKV+LIVNVAS+CG TP  Y EL  LY++YK +GF I+ FPCNQFG QEPGS+ EI +F    +   FPI  K+DVNG N  PVY  LKS+  G             +KW        K  K  +R   L  PS L  T   L
Sbjct:    5 YKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFTPQ-YKELEALYKRYKDEGFTIIGFPCNQFGHQEPGSDEEIAQFCQLNYGVTFPIMKKIDVNGGNEDPVYKFLKSQKSGMLGL-------RGIKWNFEKFLVDKKGKVYERYSSLTKPSSLSETIEEL 158
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX42_RAT (Phospholipid hydroperoxide glutathione peroxidase, nuclear OS=Rattus norvegicus GN=Gpx4 PE=2 SV=3)

HSP 1 Score: 108.612 bits (270), Expect = 3.762e-27
Identity = 53/111 (47.75%), Postives = 74/111 (66.67%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F  KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +   F ++ K+ VNG +  P++  +K
Sbjct:   95 RSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYNVRFDMYSKICVNGDDAHPLWKWMK 204

HSP 2 Score: 30.4166 bits (67), Expect = 3.762e-27
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFL 672
            P   G+LG+ +KWNF KFL
Sbjct:  207 PKGRGMLGNAIKWNFTKFL 225

HSP 3 Score: 25.0238 bits (53), Expect = 3.762e-27
Identity = 11/23 (47.83%), Postives = 14/23 (60.87%), Query Frame = 2 
Query:  674 VDKNGKVIEENPPPTSPFQIEKD 742
            +DKNG V++   P   P  IEKD
Sbjct:  226 IDKNGCVVKRYGPMEEPQVIEKD 248
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX41_RAT (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Rattus norvegicus GN=Gpx4 PE=1 SV=3)

HSP 1 Score: 108.612 bits (270), Expect = 3.811e-27
Identity = 53/111 (47.75%), Postives = 74/111 (66.67%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F  KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN EIKEFA   +   F ++ K+ VNG +  P++  +K
Sbjct:   39 RSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYNVRFDMYSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 30.4166 bits (67), Expect = 3.811e-27
Identity = 11/19 (57.89%), Postives = 14/19 (73.68%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFL 672
            P   G+LG+ +KWNF KFL
Sbjct:  151 PKGRGMLGNAIKWNFTKFL 169

HSP 3 Score: 25.0238 bits (53), Expect = 3.811e-27
Identity = 11/23 (47.83%), Postives = 14/23 (60.87%), Query Frame = 2 
Query:  674 VDKNGKVIEENPPPTSPFQIEKD 742
            +DKNG V++   P   P  IEKD
Sbjct:  170 IDKNGCVVKRYGPMEEPQVIEKD 192
[Close]
BLAST of DY258903 vs. ExPASy Swiss-Prot
Match: GPX4_MACFU (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial OS=Macaca fuscata fuscata GN=GPX4 PE=2 SV=2)

HSP 1 Score: 108.997 bits (271), Expect = 1.305e-26
Identity = 53/111 (47.75%), Postives = 76/111 (68.47%), Query Frame = 3 
Query:  282 KSLYDFTVKDIDGKDVPLSKFKGKVLLIVNVASRCGLTPSNYSELSHLYEKYKTQGFEILAFPCNQFGGQEPGSNPEIKEFACTRFKAEFPIFDKVDVNGPNTAPVYHLLK 614
            +S+++F+ KDIDG  V L K++G V ++ NVAS+ G T  NY++L  L+ +Y   G  ILAFPCNQFG QEPGSN +IKEFA   +  +F +F K+ VNG +  P++  +K
Sbjct:   39 RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGVRILAFPCNQFGKQEPGSNEKIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

HSP 2 Score: 33.113 bits (74), Expect = 1.305e-26
Identity = 16/37 (43.24%), Postives = 20/37 (54.05%), Query Frame = 1 
Query:  616 PSAGGILGDLVKWNFEKFLSGQKWKSHRRESPPYLPL 726
            P   GILG+ +KWNF KFL  +     +R  P   PL
Sbjct:  151 PKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
The following BLAST results are available for this feature:
BLAST of DY258903 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 110
Match NameE-valueIdentityDescription
GPX4_BOVIN1.582e-2748.65Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_PIG2.262e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX41_MOUSE2.262e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPO_LACLA2.705e-2747.83Glutathione peroxidase OS=Lactococcus lactis subsp... [more]
GPX4_PONPY3.490e-2748.65Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_HUMAN3.490e-2748.65Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX3_YEAST3.589e-2743.83Peroxiredoxin HYR1 OS=Saccharomyces cerevisiae GN=... [more]
GPX42_RAT3.762e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX41_RAT3.811e-2747.75Phospholipid hydroperoxide glutathione peroxidase,... [more]
GPX4_MACFU1.305e-2647.75Phospholipid hydroperoxide glutathione peroxidase,... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAI3DG11FM1 AbsDev1 Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY258903 ID=DY258903; Name=DY258903; organism=Citrus clementina; type=EST; length=947bp
GATTGCAACGCCTCTAAACCCCCGAACTGGTGTCGGACTCTATGTCGTTC
GAAGGATAACTGGCCTCACATAAACCGGATTTCACACCTAACAAAACATG
CCCTGCTGCATGGGCTACATCAATGGCGGCTTCATGGACTCCTACTAATT
CAATCAAAGCATCGATTGGGTCCGCTAAACCAGGCTTGCTTCAACATGGG
CTGTTTTCCCAATCTTCAAATTTGCCTGGGTTTTTCGTGAAACGTCGTTC
CTTTGGTGCTCACGCTACAGCTGCTACTGAGAAGTCTTTATATGACTTCA
CCGTCAAGGATATTGATGGTAAAGATGTTCCTCTTAGCAAGTTTAAGGGC
AAAGTTCTGTTGATTGTGAATGTCGCTTCAAGATGTGGCTTGACACCATC
TAATTACTCGGAGCTCTCTCACTTATATGAGAAGTACAAGACTCAAGGAT
TTGAGATTCTAGCTTTCCCTTGCAATCAATTTGGTGGGCAAGAGCCTGGA
TCAAACCCTGAGATCAAGGAATTCGCTTGTACTAGGTTTAAGGCAGAATT
TCCCATATTTGACAAGGTTGATGTAAATGGACCAAACACAGCTCCAGTTT
ACCACTTATTGAAGTCCAAGTGCTGGGGGAATTTTAGGCGACCTTGTCAA
GTGGAACTTTGAGAAGTTCTTAAGTGGACAAAAATGGAAAAGTCATCGAA
GAGAATCCCCCCCCTACCTCCCCCTTCCAAATTGAGAAGGACAACCAGAA
ACCTTGGTGGGGAATGAAACCTGACTTGACCTGCTTGGCCAAGTCTCAAA
TGTATCCACCAAAAAAAAACCAAAAAAAAGTATATGTATATTTCTGAATC
CCGCATCACCTAAAAGGAATTTTTCCCCGGCCCCCCCCGAAATGGTGAAA
GGTTTTTAAACCCCAAAAAATACAAAAATTGGAACAAATTTCCTTGC
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