DY259794

Overview
NameDY259794
Unique NameDY259794
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1076
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACT_COSCS (Actin (Fragment) OS=Costaria costata PE=3 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 8.538e-18
Identity = 30/83 (36.14%), Postives = 49/83 (59.04%), Query Frame = 1 
Query:   10 NVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            N RF  PE+ FQP+ +GM  +G+ +C  + +  C   +   LY +I+L+GG+T+FP   ER+ +EL  L P   ++K+    E
Sbjct:  208 NERFRCPEVRFQPSFIGMESSGIHDCTFKTIMKCDVDIRKDLYGNIVLSGGTTMFPGIGERMTKELTALAPSTMKIKVVAPPE 290

HSP 2 Score: 41.9726 bits (97), Expect = 8.538e-18
Identity = 19/37 (51.35%), Postives = 26/37 (70.27%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQ M ++KAEY+E+G +   R+ F
Sbjct:  295 VWIGGSILASLSTFQQMWISKAEYDESGPSIVHRKCF 331
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACTD_STRPU (Actin, cytoskeletal 2B OS=Strongylocentrotus purpuratus GN=CYIIB PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.106e-17
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 1 
Query:   10 NVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            N RF  PE +FQPA LGM  AG+ E    ++  C   +   LY + +L+GGST+FP  A+R+++E+  L P   ++KI    E
Sbjct:  253 NERFRAPEALFQPAFLGMESAGIHETCYNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADRMQKEITALAPPTMKIKIIAPPE 335

HSP 2 Score: 40.0466 bits (92), Expect = 1.106e-17
Identity = 18/37 (48.65%), Postives = 25/37 (67.57%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQ M ++K EY+E+G +   R+ F
Sbjct:  340 VWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACT2_STRFN (Actin-15B OS=Strongylocentrotus franciscanus PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.106e-17
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 1 
Query:   10 NVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            N RF  PE +FQPA LGM  AG+ E    ++  C   +   LY + +L+GGST+FP  A+R+++E+  L P   ++KI    E
Sbjct:  253 NERFRAPEALFQPAFLGMESAGIHETCYNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADRMQKEITALAPPTMKIKIIAPPE 335

HSP 2 Score: 40.0466 bits (92), Expect = 1.106e-17
Identity = 18/37 (48.65%), Postives = 25/37 (67.57%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQ M ++K EY+E+G +   R+ F
Sbjct:  340 VWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACT1_ACACA (Actin-1 OS=Acanthamoeba castellanii PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.106e-17
Identity = 32/83 (38.55%), Postives = 50/83 (60.24%), Query Frame = 1 
Query:   10 NVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            N RF  PE +FQP+ LGM  AG+ E    ++  C   +   LY +++L+GG+T+FP  A+R+++EL  L P   ++KI    E
Sbjct:  252 NERFRAPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMQKELTALAPSTMKIKIIAPPE 334

HSP 2 Score: 40.0466 bits (92), Expect = 1.106e-17
Identity = 18/37 (48.65%), Postives = 25/37 (67.57%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQ M ++K EY+E+G +   R+ F
Sbjct:  339 VWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF 375
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACT1_AEDAE (Actin-1 OS=Aedes aegypti GN=ACT-1 PE=2 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 1.106e-17
Identity = 29/83 (34.94%), Postives = 49/83 (59.04%), Query Frame = 1 
Query:   10 NVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            N RF  PE +FQP+ LGM   G+ E +  ++  C   +   LY + +L+GG+T++P  A+R+++E+  L P   ++KI    E
Sbjct:  253 NERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSSIKIKIIAPPE 335

HSP 2 Score: 43.1282 bits (100), Expect = 1.106e-17
Identity = 20/37 (54.05%), Postives = 26/37 (70.27%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQAM ++K EY+E+G A   R+ F
Sbjct:  340 VWIGGSILASLSTFQAMWISKQEYDESGPAIVHRKCF 376
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACTA_STRPU (Actin, cytoskeletal 1A OS=Strongylocentrotus purpuratus GN=CYIA PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.439e-17
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 1 
Query:   10 NVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            N RF  PE +FQPA LGM  AG+ E    ++  C   +   LY + +L+GGST+FP  A+R+++E+  L P   ++KI    E
Sbjct:  253 NERFRCPEALFQPAFLGMESAGIHETCYNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADRMQKEITALAPPTMKIKIIAPPE 335

HSP 2 Score: 40.0466 bits (92), Expect = 1.439e-17
Identity = 18/37 (48.65%), Postives = 25/37 (67.57%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQ M ++K EY+E+G +   R+ F
Sbjct:  340 VWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACTA_PHYPO (Actin, plasmodial isoform OS=Physarum polycephalum GN=ARDA PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 1.439e-17
Identity = 32/83 (38.55%), Postives = 50/83 (60.24%), Query Frame = 1 
Query:   10 NVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            N RF  PE +FQP+ LGM  AG+ E    ++  C   +   LY +++L+GG+T+FP  A+R+++EL  L P   ++KI    E
Sbjct:  253 NERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMQKELTALAPSTMKIKIIAPPE 335

HSP 2 Score: 40.0466 bits (92), Expect = 1.439e-17
Identity = 18/37 (48.65%), Postives = 25/37 (67.57%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQ M ++K EY+E+G +   R+ F
Sbjct:  340 VWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF 376
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ARP6_DROME (Actin-like protein 13E OS=Drosophila melanogaster GN=Actr13E PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.867e-17
Identity = 36/85 (42.35%), Postives = 53/85 (62.35%), Query Frame = 1 
Query:    4 LTNVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            L N RF VPE++F P+D+G+ Q G+ E +   + +C    H  L  +I++ GGS  FP F  RL+R+LR LVPDD +V +   E+
Sbjct:  275 LCNERFTVPELLFNPSDIGVQQVGIPEAVADCLKACPWEAHRELLLNILIVGGSAQFPGFLPRLKRDLRALVPDDLEVSLICPED 359

HSP 2 Score: 31.9574 bits (71), Expect = 1.867e-17
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 3 
Query:  345 PLLGVWRGGSLLASSPDFQAMCVTKAEYEENG 440
            P+   W GG  +A+SP+F+    T+ +YEE G
Sbjct:  360 PVRYAWYGGKEVATSPNFEEFVYTQDDYEEYG 391
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ARP6_YARLI (Actin-like protein ARP6 OS=Yarrowia lipolytica GN=ARP6 PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.869e-17
Identity = 30/79 (37.97%), Postives = 49/79 (62.03%), Query Frame = 1 
Query:    4 LTNVRFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVK 240
            L N RF +PE++F P+ + + Q+GL E I  ++ +      S+L  +I++ GG    P F  RLE++LRPL+P +Y +K
Sbjct:  251 LGNERFTMPEILFDPSIIDLQQSGLCETIAESIQASPKEFRSLLCSNIVIVGGCAKLPGFQSRLEKDLRPLIPSEYHMK 329

HSP 2 Score: 41.2022 bits (95), Expect = 1.869e-17
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 3 
Query:  363 RGGSLLASSPDFQAMCVTKAEYEENGSARCRRRF 464
            +GG +L   P F  +CVTK EY+E G   CR+RF
Sbjct:  342 QGGKVLVDQPGFSNLCVTKQEYDEYGWRLCRQRF 375
[Close]
BLAST of DY259794 vs. ExPASy Swiss-Prot
Match: ACT1_TOBAC (Actin OS=Nicotiana tabacum PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.870e-17
Identity = 30/81 (37.04%), Postives = 48/81 (59.26%), Query Frame = 1 
Query:   16 RFLVPEMIFQPADLGMNQAGLAECIVRAVNSCHPYLHSVLYESIILTGGSTLFPRFAERLERELRPLVPDDYQVKITTQEE 258
            RF  PE++FQP+ +GM  AG+ E    ++  C   +   LY  I+L+GGST+FP  A+R+ +E+  L P   ++K+    E
Sbjct:  256 RFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGKIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPE 336

HSP 2 Score: 41.5874 bits (96), Expect = 1.870e-17
Identity = 19/37 (51.35%), Postives = 25/37 (67.57%), Query Frame = 3 
Query:  357 VWRGGSLLASSPDFQAMCVTKAEYEENGSARCRRRFF 467
            VW GGS+LAS   FQ M + KAEY+E+G +   R+ F
Sbjct:  341 VWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 377
The following BLAST results are available for this feature:
BLAST of DY259794 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 373
Match NameE-valueIdentityDescription
ACT_COSCS8.538e-1836.14Actin (Fragment) OS=Costaria costata PE=3 SV=2[more]
ACTD_STRPU1.106e-1739.76Actin, cytoskeletal 2B OS=Strongylocentrotus purpu... [more]
ACT2_STRFN1.106e-1739.76Actin-15B OS=Strongylocentrotus franciscanus PE=2 ... [more]
ACT1_ACACA1.106e-1738.55Actin-1 OS=Acanthamoeba castellanii PE=3 SV=1[more]
ACT1_AEDAE1.106e-1734.94Actin-1 OS=Aedes aegypti GN=ACT-1 PE=2 SV=2[more]
ACTA_STRPU1.439e-1739.76Actin, cytoskeletal 1A OS=Strongylocentrotus purpu... [more]
ACTA_PHYPO1.439e-1738.55Actin, plasmodial isoform OS=Physarum polycephalum... [more]
ARP6_DROME1.867e-1742.35Actin-like protein 13E OS=Drosophila melanogaster ... [more]
ARP6_YARLI1.869e-1737.97Actin-like protein ARP6 OS=Yarrowia lipolytica GN=... [more]
ACT1_TOBAC1.870e-1737.04Actin OS=Nicotiana tabacum PE=3 SV=1[more]

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Properties
Property NameValue
Genbank descriptionKN0AAP10YD12FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY259794 ID=DY259794; Name=DY259794; organism=Citrus clementina; type=EST; length=1076bp
GACTTGACAAATGTACGCTTTCTTGTCCCAGAGATGATCTTTCAACCTGC
AGATTTGGGAATGAACCAGGCTGGACTAGCTGAGTGCATTGTTCGAGCTG
TTAATTCTTGCCATCCTTATCTTCATTCTGTACTTTATGAAAGCATTATA
TTAACTGGTGGAAGCACGTTATTCCCTCGATTTGCTGAAAGACTAGAAAG
GGAGCTTCGGCCTCTAGTCCCTGATGACTATCAAGTGAAAATAACAACCC
AAGAAGAGTATTCACATCCTCCTTAAAATACTAATGACTTTTTCTTTAGA
AGTTACTTCTTCCCAATTGGTAAAACATTTTCGTGCTTTCCAGTCCCTTA
TTAGGTGTCTGGCGAGGAGGATCACTTTTGGCTTCCAGTCCTGATTTTCA
AGCAATGTGTGTCACCAAGGCTGAGTATGAGGAGAATGGATCTGCTCGAT
GTCGCAGGCGATTCTTTCATTGACTTCAAATATTTCTAAAGCTTTGTGGC
AATTAAATGGTGCACGAGGACTTAAGCTGCCGTGGATGCACTGTCTTCTG
TTGAACAAAACAAGGTGGATCCAGGTTTTGATGCCTTGTAAATGTTGGGA
AAACATCTGGACTTCTACACACAGCTTGTCCGGGTATTGGAACAGAGAAA
ATATGGCACCTTGGCAGTTTCACCCTCTCAAGGTGATGGGTCGAGGTTTA
ATCCCTTGGCTATAGAAAGTGACTGACATGCTCAAATCCCGGATCATTCA
AACCCAAAGGCAAAACTAAACAAACGGGATCCCCCCCATTATGGAAGGTC
CCCCCTGGTACCGGGTTAAAACCCCTTTTGTTGAAACCCTTCTTTCCCCC
CCCTCCATTTGTTTTTTTACCAGGTGGGCCCCTAAAGGGAGGAAAGGGTG
AAACAGGGGGAAGTCCATTTCTCGGAAAATTTTTTTGTAATTTGGGGCTT
TTTTTCTCACCCTTTTGAATAAAAATGTAAAAACCAAAAGCCCTTGAAAA
AAAATTCTTGCTTCCTTCCCTTTAAACTCCAAAGGGTTGGGGGATATACC
CTTTAAAGGGGGGGAGCAACCTCAAA
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