DY260313

Overview
NameDY260313
Unique NameDY260313
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1301
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D3_PONAB (Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.088e-29
Identity = 59/112 (52.68%), Postives = 73/112 (65.18%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D+M ++   I+GP  SPY+GGVF L +  P +YP   PKV F T+IYHPNI+  G ICLDIL+ +WSPAL I  VLLSI +LL  PNP
Sbjct:    4 KRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 3.088e-29
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL   I + +KT+  +    ++EWT+ YA
Sbjct:  115 PDDPLVPEIARIYKTDRDKYNRISREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D3_MOUSE (Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.088e-29
Identity = 59/112 (52.68%), Postives = 73/112 (65.18%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D+M ++   I+GP  SPY+GGVF L +  P +YP   PKV F T+IYHPNI+  G ICLDIL+ +WSPAL I  VLLSI +LL  PNP
Sbjct:    4 KRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 3.088e-29
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL   I + +KT+  +    ++EWT+ YA
Sbjct:  115 PDDPLVPEIARIYKTDRDKYNRISREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D3_MACFA (Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fascicularis GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.088e-29
Identity = 59/112 (52.68%), Postives = 73/112 (65.18%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D+M ++   I+GP  SPY+GGVF L +  P +YP   PKV F T+IYHPNI+  G ICLDIL+ +WSPAL I  VLLSI +LL  PNP
Sbjct:    4 KRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 3.088e-29
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL   I + +KT+  +    ++EWT+ YA
Sbjct:  115 PDDPLVPEIARIYKTDRDKYNRISREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D3_HUMAN (Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens GN=UBE2D3 PE=1 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.088e-29
Identity = 59/112 (52.68%), Postives = 73/112 (65.18%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D+M ++   I+GP  SPY+GGVF L +  P +YP   PKV F T+IYHPNI+  G ICLDIL+ +WSPAL I  VLLSI +LL  PNP
Sbjct:    4 KRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 3.088e-29
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL   I + +KT+  +    ++EWT+ YA
Sbjct:  115 PDDPLVPEIARIYKTDRDKYNRISREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D2_PIG (Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa GN=UBE2D2 PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.088e-29
Identity = 59/112 (52.68%), Postives = 73/112 (65.18%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D+M ++   I+GP  SPY+GGVF L +  P +YP   PKV F T+IYHPNI+  G ICLDIL+ +WSPAL I  VLLSI +LL  PNP
Sbjct:    4 KRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 3.088e-29
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL   I + +KT+  +    ++EWT+ YA
Sbjct:  115 PDDPLVPEIARIYKTDRDKYNRISREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UBE2T_BOVIN (Ubiquitin-conjugating enzyme E2 T OS=Bos taurus GN=UBE2T PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.487e-28
Identity = 58/115 (50.43%), Postives = 72/115 (62.61%), Query Frame = 1 
Query:   73 RIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDIL----KDKWSPALQIRTVLLSIQALLSAPNP 405
            R+ +E   L +EP PGI+     D M      ILG   +PYE GVFKLE+ +PE YP   P++RFLT IYHPNID  GRICLD+L    K  W P+L I T+L  IQ L++ PNP
Sbjct:    6 RLKRELSLLAAEPPPGITCWQDGDRMEDLRAQILGGANTPYEKGVFKLEVHIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATLLTCIQQLMAEPNP 120

HSP 2 Score: 30.0314 bits (66), Expect = 1.487e-28
Identity = 12/32 (37.50%), Postives = 19/32 (59.38%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL  +I   +K N+    + A++WT  +A
Sbjct:  120 PDDPLMADISSEFKYNKPVFFKNARQWTEKHA 151
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D4_HUMAN (Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens GN=UBE2D4 PE=1 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.505e-28
Identity = 58/112 (51.79%), Postives = 73/112 (65.18%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D++ ++   I+GP  SPY+GGVF L +  P +YP   PKV F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNP
Sbjct:    4 KRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNP 115

HSP 2 Score: 30.0314 bits (66), Expect = 1.505e-28
Identity = 13/32 (40.62%), Postives = 18/32 (56.25%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL   I   +K +  +    A+EWT+ YA
Sbjct:  115 PDDPLVPEIAHTYKADREKYNRLAREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D3_BOVIN (Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.959e-28
Identity = 58/112 (51.79%), Postives = 72/112 (64.29%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D+M ++   I+GP  SPY+GGVF L +  P +YP   PKV F T+IYHPNI+    ICLDIL+ +WSPAL I  VLLSI +LL  PNP
Sbjct:    4 KRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNDSICLDILRSQWSPALTISKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 1.959e-28
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL   I + +KT+  +    ++EWT+ YA
Sbjct:  115 PDDPLVPEIARIYKTDRDKYNRISREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 5.565e-28
Identity = 65/132 (49.24%), Postives = 79/132 (59.85%), Query Frame = 1 
Query:   22 KKQKKSILEMANSNL----PRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            KK++  +    NS L     +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    EYP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP
Sbjct:   30 KKKESKVSMSKNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP 161

HSP 2 Score: 31.187 bits (69), Expect = 5.565e-28
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYAS 502
            P DPL  +I   + TN A+    A++WT+ YA+
Sbjct:  161 PADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 193
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 5.565e-28
Identity = 65/132 (49.24%), Postives = 79/132 (59.85%), Query Frame = 1 
Query:   22 KKQKKSILEMANSNL----PRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            KK++  +    NS L     +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    EYP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP
Sbjct:   30 KKKESKVSMSKNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP 161

HSP 2 Score: 31.187 bits (69), Expect = 5.565e-28
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYAS 502
            P DPL  +I   + TN A+    A++WT+ YA+
Sbjct:  161 PADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 193
The following BLAST results are available for this feature:
BLAST of DY260313 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 240
Match NameE-valueIdentityDescription
UB2D3_PONAB3.088e-2952.68Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii... [more]
UB2D3_MOUSE3.088e-2952.68Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus... [more]
UB2D3_MACFA3.088e-2952.68Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fasci... [more]
UB2D3_HUMAN3.088e-2952.68Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens... [more]
UB2D2_PIG3.088e-2952.68Ubiquitin-conjugating enzyme E2 D2 OS=Sus scrofa G... [more]
UBE2T_BOVIN1.487e-2850.43Ubiquitin-conjugating enzyme E2 T OS=Bos taurus GN... [more]
UB2D4_HUMAN1.505e-2851.79Ubiquitin-conjugating enzyme E2 D4 OS=Homo sapiens... [more]
UB2D3_BOVIN1.959e-2851.79Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus G... [more]
UB2E1_MOUSE5.565e-2849.24Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN5.565e-2849.24Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP12YB21FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY260313 ID=DY260313; Name=DY260313; organism=Citrus clementina; type=EST; length=1301bp
CTCTGCAACAACCCTTAGGTTAAAAAACAAAAAAAATCGATCTTGGAAAT
GGCGAACAGTAATCTCCCTCGACGTATCATCAAGGAAACACAGCGTCTCC
TCAGTGAACCAGCACCAGGAATAAGTGCATCTCCCTCGGAAGATAATATG
CGATACTTTAATGTGATGATCCTTGGTCCAACACAATCTCCATATGAAGG
AGGGGTGTTCAAATTGGAATTATTTTTGCCTGAAGAATATCCAATGGCTG
CTCCAAAGGTCCGATTTCTTACCAAGATATATCATCCTAACATTGATAAG
CTTGGAAGGATTTGTCTTGATATTCTGAAAGACAAGTGGAGTCCAGCTCT
CCAGATTCGGACTGTACTTTTGAGCATTCAAGCACTTCTGAGTGCTCCTA
ACCCCCGATGATCCTCTCTCTGAGAACATTCGCAAACACTGGAAAACAAA
TGAGGCCCAAGCGGTTGAAACAGCGAAGGAATGGACACGTTTATATGCCA
GTGGTGCTTGATGACATGGTGGAAAATATCCCTTCCTCCAAATAACCATT
GTGCAATGTATGCCTACTCACTGTGTTTAAACATGAAAATTTGGAAAAAA
TGAATTGCTGTTCGAACCATTCCGATTTTGCCATCTCCCAAAAAAAAAAA
AAAAAAAAAAACCCCCCCTTCCTTGGACCACTGTCGTCACTATACCACCG
CCTTCTTCTATCCAATTTCTTGCCCCCAACCGCCGCCCGTACCACCCCAT
AACAAATCCTTTAATCTTGCAACCCCAGTGATAGACCCAGTTCCATACAA
GCGACCATACATCTCCTAACACAACCGCCTAACCAGCCCAAGCCCAATCC
CCTATCTCCCCCAACTCCTCACCTAAACCATTATCCTCTACACCCAATAC
CTATCTCCCTCGCCCCCCAAATTAAACCCCCTCCAACACACAAATAACCA
GGCATGTAAACGATCATATTAACAACTGCCAAGAACTACAACTACCCAAC
AGTCCCTACACAACTCGATTTACGTATCCTTAACAGTTACTACTCCTCAT
CATTACCGTCTGCCCCTATCTACCGTCATATCTAAAACTGCGCTCACCAC
GCAAAATCCTCAATACCATTGTACCCGCCACACATCACGCCAGTATCTTC
CCAACACCTCTACCACGTACACTACCTCCCTCCACACTACATCTCGTCCC
GTCCTTTCTTCCTCCCTCGACATGCCTTGAAGTTCTCTCACATCACAGTA
CCATCTATCACTCTCACTCCAATGCAATATGTACACTCTTTACTTCTGAT
A
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