DY260313

Overview
NameDY260313
Unique NameDY260313
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1301
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D1_MOUSE (Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus GN=Ube2d1 PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 9.543e-28
Identity = 56/112 (50.00%), Postives = 72/112 (64.29%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D++ ++   I+GP  S Y+GGVF L +  P +YP   PK+ F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNP
Sbjct:    4 KRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 9.543e-28
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL  +I + +K+++ +    A+EWT+ YA
Sbjct:  115 PDDPLVPDIAQIYKSDKEKYNRHAREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D1_HUMAN (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 9.543e-28
Identity = 56/112 (50.00%), Postives = 72/112 (64.29%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D++ ++   I+GP  S Y+GGVF L +  P +YP   PK+ F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNP
Sbjct:    4 KRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 9.543e-28
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL  +I + +K+++ +    A+EWT+ YA
Sbjct:  115 PDDPLVPDIAQIYKSDKEKYNRHAREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2D1_BOVIN (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 9.543e-28
Identity = 56/112 (50.00%), Postives = 72/112 (64.29%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI KE   L  +P    SA P  D++ ++   I+GP  S Y+GGVF L +  P +YP   PK+ F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNP
Sbjct:    4 KRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNP 115

HSP 2 Score: 31.187 bits (69), Expect = 9.543e-28
Identity = 13/32 (40.62%), Postives = 22/32 (68.75%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYA 499
            PDDPL  +I + +K+++ +    A+EWT+ YA
Sbjct:  115 PDDPLVPDIAQIYKSDKEKYNRHAREWTQKYA 146
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2E3_MOUSE (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 113.235 bits (282), Expect = 3.519e-27
Identity = 60/116 (51.72%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:   58 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP 175

HSP 2 Score: 30.8018 bits (68), Expect = 3.519e-27
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYAS 502
            P DPL  +I   + TN A+    A++WT+ YA+
Sbjct:  175 PADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2E3_HUMAN (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.519e-27
Identity = 60/116 (51.72%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:   58 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP 175

HSP 2 Score: 30.8018 bits (68), Expect = 3.519e-27
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYAS 502
            P DPL  +I   + TN A+    A++WT+ YA+
Sbjct:  175 PADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2E3_BOVIN (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 3.519e-27
Identity = 60/116 (51.72%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:   58 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP 175

HSP 2 Score: 30.8018 bits (68), Expect = 3.519e-27
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYAS 502
            P DPL  +I   + TN A+    A++WT+ YA+
Sbjct:  175 PADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 3.522e-27
Identity = 60/116 (51.72%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:   58 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP
Sbjct:   54 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP 169

HSP 2 Score: 31.187 bits (69), Expect = 3.522e-27
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYAS 502
            P DPL  +I   + TN A+    A++WT+ YA+
Sbjct:  169 PADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 201
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 3.522e-27
Identity = 60/116 (51.72%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:   58 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP
Sbjct:   54 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNP 169

HSP 2 Score: 31.187 bits (69), Expect = 3.522e-27
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYAS 502
            P DPL  +I   + TN A+    A++WT+ YA+
Sbjct:  169 PADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 201
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.335e-26
Identity = 54/113 (47.79%), Postives = 73/113 (64.60%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASP-SEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            +RI +E + +   P    SA P +E+++ ++   I+GP  SPY GGVF + +    +YP   PKV F TK+YHPNID  G ICLDILK++WSPA     VLLSI +LL+ PNP
Sbjct:    4 KRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNP 116

HSP 2 Score: 31.5722 bits (70), Expect = 1.335e-26
Identity = 13/34 (38.24%), Postives = 22/34 (64.71%), Query Frame = 2 
Query:  404 PDDPLSENIRKHWKTNEAQAVETAKEWTRLYASG 505
            P+DPL   I   +K ++++   TA++WT+ YA G
Sbjct:  116 PNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149
[Close]
BLAST of DY260313 vs. ExPASy Swiss-Prot
Match: UBE2B_RAT (Ubiquitin-conjugating enzyme E2 B OS=Rattus norvegicus GN=Ube2b PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.129e-26
Identity = 53/112 (47.32%), Postives = 79/112 (70.54%), Query Frame = 1 
Query:   70 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNP 405
            RR++++ +RL  +P  G+S +PSE+N+  +N +I GP  +P+E G FKL +   EEYP   P VRFL+K++HPN+   G ICLDIL+++WSP   + ++L SIQ+LL  PNP
Sbjct:    7 RRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNP 118
The following BLAST results are available for this feature:
BLAST of DY260313 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 240
Match NameE-valueIdentityDescription
UB2D1_MOUSE9.543e-2850.00Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus... [more]
UB2D1_HUMAN9.543e-2850.00Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens... [more]
UB2D1_BOVIN9.543e-2850.00Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus G... [more]
UB2E3_MOUSE3.519e-2751.72Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus... [more]
UB2E3_HUMAN3.519e-2751.72Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens... [more]
UB2E3_BOVIN3.519e-2751.72Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus G... [more]
UB2E2_MOUSE3.522e-2751.72Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN3.522e-2751.72Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UBC12_ARATH1.335e-2647.79Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
UBE2B_RAT2.129e-2647.32Ubiquitin-conjugating enzyme E2 B OS=Rattus norveg... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP12YB21FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY260313 ID=DY260313; Name=DY260313; organism=Citrus clementina; type=EST; length=1301bp
CTCTGCAACAACCCTTAGGTTAAAAAACAAAAAAAATCGATCTTGGAAAT
GGCGAACAGTAATCTCCCTCGACGTATCATCAAGGAAACACAGCGTCTCC
TCAGTGAACCAGCACCAGGAATAAGTGCATCTCCCTCGGAAGATAATATG
CGATACTTTAATGTGATGATCCTTGGTCCAACACAATCTCCATATGAAGG
AGGGGTGTTCAAATTGGAATTATTTTTGCCTGAAGAATATCCAATGGCTG
CTCCAAAGGTCCGATTTCTTACCAAGATATATCATCCTAACATTGATAAG
CTTGGAAGGATTTGTCTTGATATTCTGAAAGACAAGTGGAGTCCAGCTCT
CCAGATTCGGACTGTACTTTTGAGCATTCAAGCACTTCTGAGTGCTCCTA
ACCCCCGATGATCCTCTCTCTGAGAACATTCGCAAACACTGGAAAACAAA
TGAGGCCCAAGCGGTTGAAACAGCGAAGGAATGGACACGTTTATATGCCA
GTGGTGCTTGATGACATGGTGGAAAATATCCCTTCCTCCAAATAACCATT
GTGCAATGTATGCCTACTCACTGTGTTTAAACATGAAAATTTGGAAAAAA
TGAATTGCTGTTCGAACCATTCCGATTTTGCCATCTCCCAAAAAAAAAAA
AAAAAAAAAAACCCCCCCTTCCTTGGACCACTGTCGTCACTATACCACCG
CCTTCTTCTATCCAATTTCTTGCCCCCAACCGCCGCCCGTACCACCCCAT
AACAAATCCTTTAATCTTGCAACCCCAGTGATAGACCCAGTTCCATACAA
GCGACCATACATCTCCTAACACAACCGCCTAACCAGCCCAAGCCCAATCC
CCTATCTCCCCCAACTCCTCACCTAAACCATTATCCTCTACACCCAATAC
CTATCTCCCTCGCCCCCCAAATTAAACCCCCTCCAACACACAAATAACCA
GGCATGTAAACGATCATATTAACAACTGCCAAGAACTACAACTACCCAAC
AGTCCCTACACAACTCGATTTACGTATCCTTAACAGTTACTACTCCTCAT
CATTACCGTCTGCCCCTATCTACCGTCATATCTAAAACTGCGCTCACCAC
GCAAAATCCTCAATACCATTGTACCCGCCACACATCACGCCAGTATCTTC
CCAACACCTCTACCACGTACACTACCTCCCTCCACACTACATCTCGTCCC
GTCCTTTCTTCCTCCCTCGACATGCCTTGAAGTTCTCTCACATCACAGTA
CCATCTATCACTCTCACTCCAATGCAATATGTACACTCTTTACTTCTGAT
A
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