DY260467

Overview
NameDY260467
Unique NameDY260467
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1301
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig1545 contig Ccv1_Contig1545:1..1301. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB5A_MACFA (Ras-related protein Rab-5A OS=Macaca fascicularis GN=RAB5A PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.902e-24
Identity = 60/151 (39.74%), Postives = 92/151 (60.93%), Query Frame = 1 
Query:  133 KVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            K+++LG+S VGK+SL+ ++V  +F    ++TIGA FLT+ V  +D     +IWDTAGQER+ SL   +YRGA   ++VYD+ + +SF    NW +E   QASP    N    + GNK D+   N R V  ++A+++ A   ++ + ET  K
Sbjct:   22 KLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASP----NIVIALSGNKADL--ANKRAVDFQEAQSY-ADDNSLLFMETSAK 165

HSP 2 Score: 24.2534 bits (51), Expect = 1.902e-24
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  563 LTLRPSAKEGINVEEAFQCIAKNALKS 643
            L +  SAK  +NV E F  IAK   K+
Sbjct:  158 LFMETSAKTSMNVNEIFMAIAKKLPKN 184
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB5A_HUMAN (Ras-related protein Rab-5A OS=Homo sapiens GN=RAB5A PE=1 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 1.902e-24
Identity = 60/151 (39.74%), Postives = 92/151 (60.93%), Query Frame = 1 
Query:  133 KVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            K+++LG+S VGK+SL+ ++V  +F    ++TIGA FLT+ V  +D     +IWDTAGQER+ SL   +YRGA   ++VYD+ + +SF    NW +E   QASP    N    + GNK D+   N R V  ++A+++ A   ++ + ET  K
Sbjct:   22 KLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASP----NIVIALSGNKADL--ANKRAVDFQEAQSY-ADDNSLLFMETSAK 165

HSP 2 Score: 24.2534 bits (51), Expect = 1.902e-24
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  563 LTLRPSAKEGINVEEAFQCIAKNALKS 643
            L +  SAK  +NV E F  IAK   K+
Sbjct:  158 LFMETSAKTSMNVNEIFMAIAKKLPKN 184
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB5A_CANFA (Ras-related protein Rab-5A OS=Canis familiaris GN=RAB5A PE=1 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.902e-24
Identity = 60/151 (39.74%), Postives = 92/151 (60.93%), Query Frame = 1 
Query:  133 KVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            K+++LG+S VGK+SL+ ++V  +F    ++TIGA FLT+ V  +D     +IWDTAGQER+ SL   +YRGA   ++VYD+ + +SF    NW +E   QASP    N    + GNK D+   N R V  ++A+++ A   ++ + ET  K
Sbjct:   22 KLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASP----NIVIALSGNKADL--ANKRAVDFQEAQSY-ADDNSLLFMETSAK 165

HSP 2 Score: 24.2534 bits (51), Expect = 1.902e-24
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  563 LTLRPSAKEGINVEEAFQCIAKNALKS 643
            L +  SAK  +NV E F  IAK   K+
Sbjct:  158 LFMETSAKTSMNVNEIFMAIAKKLPKN 184
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB5A_BOVIN (Ras-related protein Rab-5A OS=Bos taurus GN=RAB5A PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.902e-24
Identity = 60/151 (39.74%), Postives = 92/151 (60.93%), Query Frame = 1 
Query:  133 KVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            K+++LG+S VGK+SL+ ++V  +F    ++TIGA FLT+ V  +D     +IWDTAGQER+ SL   +YRGA   ++VYD+ + +SF    NW +E   QASP    N    + GNK D+   N R V  ++A+++ A   ++ + ET  K
Sbjct:   22 KLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASP----NIVIALSGNKADL--ANKRAVDFQEAQSY-ADDNSLLFMETSAK 165

HSP 2 Score: 24.2534 bits (51), Expect = 1.902e-24
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  563 LTLRPSAKEGINVEEAFQCIAKNALKS 643
            L +  SAK  +NV E F  IAK   K+
Sbjct:  158 LFMETSAKTSMNVNEIFMAIAKKLPKN 184
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB8A_RAT (Ras-related protein Rab-8A OS=Rattus norvegicus GN=Rab8a PE=2 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 1.904e-24
Identity = 61/153 (39.87%), Postives = 96/153 (62.75%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW       AS +D +    ++LGNK DV+  + R VS+++        G I + ET  K
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHAS-ADVEK---MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAK 153

HSP 2 Score: 22.7126 bits (47), Expect = 1.904e-24
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKN 631
            +  SAK  INVE AF  +A++
Sbjct:  148 METSAKANINVENAFFTLARD 168
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB8A_MOUSE (Ras-related protein Rab-8A OS=Mus musculus GN=Rab8a PE=1 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 1.904e-24
Identity = 61/153 (39.87%), Postives = 96/153 (62.75%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW       AS +D +    ++LGNK DV+  + R VS+++        G I + ET  K
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHAS-ADVEK---MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAK 153

HSP 2 Score: 22.7126 bits (47), Expect = 1.904e-24
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKN 631
            +  SAK  INVE AF  +A++
Sbjct:  148 METSAKANINVENAFFTLARD 168
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB8A_MACFA (Ras-related protein Rab-8A OS=Macaca fascicularis GN=RAB8A PE=2 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.904e-24
Identity = 61/153 (39.87%), Postives = 96/153 (62.75%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW       AS +D +    ++LGNK DV+  + R VS+++        G I + ET  K
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHAS-ADVEK---MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAK 153

HSP 2 Score: 22.7126 bits (47), Expect = 1.904e-24
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKN 631
            +  SAK  INVE AF  +A++
Sbjct:  148 METSAKANINVENAFFTLARD 168
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB8A_HUMAN (Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8A PE=1 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.904e-24
Identity = 61/153 (39.87%), Postives = 96/153 (62.75%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW       AS +D +    ++LGNK DV+  + R VS+++        G I + ET  K
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHAS-ADVEK---MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAK 153

HSP 2 Score: 22.7126 bits (47), Expect = 1.904e-24
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKN 631
            +  SAK  INVE AF  +A++
Sbjct:  148 METSAKANINVENAFFTLARD 168
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB8A_CANFA (Ras-related protein Rab-8A OS=Canis familiaris GN=RAB8A PE=2 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.904e-24
Identity = 61/153 (39.87%), Postives = 96/153 (62.75%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++    F++ + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSFDN+ NW       AS +D +    ++LGNK DV+  + R VS+++        G I + ET  K
Sbjct:    8 LFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHAS-ADVEK---MILGNKCDVN--DKRQVSKERGEKLALDYG-IKFMETSAK 153

HSP 2 Score: 22.7126 bits (47), Expect = 1.904e-24
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKN 631
            +  SAK  INVE AF  +A++
Sbjct:  148 METSAKANINVENAFFTLARD 168
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: YPTM2_MAIZE (GTP-binding protein YPTM2 OS=Zea mays GN=YPTM2 PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.905e-24
Identity = 60/153 (39.22%), Postives = 97/153 (63.40%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGK+ L+ ++ +  + + Y +TIG DF  + V+ + +   LQIWDTAGQERF+++  ++YRGA   ++VYDV   +SF+N+  W  E    AS    DN   +++GNK D+    ++VV+ + A+A+    G IP+ ET  K
Sbjct:    8 LFKLLLIGDSGVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYAS----DNVNKLLVGNKSDLTA--NKVVATETAKAFADEMG-IPFMETSAK 153

HSP 2 Score: 20.7866 bits (42), Expect = 1.905e-24
Identity = 8/19 (42.11%), Postives = 12/19 (63.16%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIA 625
            +  SAK   NV++AF  +A
Sbjct:  148 METSAKNATNVQQAFMAMA 166
The following BLAST results are available for this feature:
BLAST of DY260467 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RAB5A_MACFA1.902e-2439.74Ras-related protein Rab-5A OS=Macaca fascicularis ... [more]
RAB5A_HUMAN1.902e-2439.74Ras-related protein Rab-5A OS=Homo sapiens GN=RAB5... [more]
RAB5A_CANFA1.902e-2439.74Ras-related protein Rab-5A OS=Canis familiaris GN=... [more]
RAB5A_BOVIN1.902e-2439.74Ras-related protein Rab-5A OS=Bos taurus GN=RAB5A ... [more]
RAB8A_RAT1.904e-2439.87Ras-related protein Rab-8A OS=Rattus norvegicus GN... [more]
RAB8A_MOUSE1.904e-2439.87Ras-related protein Rab-8A OS=Mus musculus GN=Rab8... [more]
RAB8A_MACFA1.904e-2439.87Ras-related protein Rab-8A OS=Macaca fascicularis ... [more]
RAB8A_HUMAN1.904e-2439.87Ras-related protein Rab-8A OS=Homo sapiens GN=RAB8... [more]
RAB8A_CANFA1.904e-2439.87Ras-related protein Rab-8A OS=Canis familiaris GN=... [more]
YPTM2_MAIZE1.905e-2439.22GTP-binding protein YPTM2 OS=Zea mays GN=YPTM2 PE=... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP12YJ07FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY260467 ID=DY260467; Name=DY260467; organism=Citrus clementina; type=EST; length=1301bp
CGCACGCAGAGCCTCTTTAACAACTACGGAGCACGTCAAAACGATCAACT
GATTTGCGTTTGGAGGTAGATCGTTCGATCGATTGACCGATTTTGCCCCA
GAACGATGCCGTCTCGTAGAAGAACTCTCCTCAAGGTCATCATCCTCGGC
GACAGCGGGGTGGGCAAGACCTCTTTGATGAATCAATATGTAAATAAGAA
GTTTAGCAATCAGTACAAGGCGACCATCGGAGCTGATTTCTTGACAAAGG
AAGTGCAGTTTGAAGATAGGCTCTTCACCTTACAGATCTGGGATACAGCT
GGTCAGGAAAGATTCCAAAGCCTGGGTGTTGCTTTCTACCGAGGTGCAGA
TTGCTGTGTACTTGTGTATGATGTAAATTCTATGAAGTCCTTTGACAACC
TCAATAACTGGAGAGAAGAATTCCTCATTCAGGCTAGCCCTTCGGATCCA
GACAATTTCCCCTTTGTTGTTTTAGGAAACAAGATTGATGTAGATGGTGG
AAACAGTAGGGTGGTTTCGGAGAAGAAGGCTCGAGCCTGGTGTGCCTCCA
AAGGAAATATTCCTTACTTTGAGACCCTCTGCAAAAGAAGGCATTAATGT
GGAAGAAGCTTTCCAGTGCATAGCTAAAAATGCTTTGAAGAGTGGGGAGG
AGGAACAGATACACCTTGCCGGACACCATTGATGTTGGAACCCGCCCCCC
GCCCAGGGCCAGTGGAAGCAAGTGCCAAAGATATGGGCCAATTTTCTTCA
AGGACCCTCAACCACTTACAGAAATTGATTGGCCCTTTATAACATTTTTC
ATGGGGAACGCAAAGGGTTGCGGTGCAATCTAATGCTTGGGGCCAACCAA
CCCGGTCCCTCCCACCCACCTCCCCTCATCTGGTCCCTGGACTCGCCCCT
CAACCCCACCCATCCACTCGTACACTGCCTCCCCCCCCCCGCACGCCATC
CTGACTCTCCCCTATCCCGCGCAACCCGCCCGTTGGTCCTCACCCCCCTC
TATGCATCTCCCCCACCAGGACCAGCCATACAAGATCCACAGGCACAACA
CTTCCCTCTCTACACATCACTCTGCCCCTTCATGCCCAACCACTACGACC
TACATCACAACTCCCATCCACGACACAACCACCACATCCCCCCCCCCACC
ACACACCCCGACTCACTACTCCAGACACCTCCCCCCGCTGCACTCCTCCA
CAGACCCAGGCCCTGCATACCACACGCCGCCTGAACACACCACACCCCAA
CACTACATGCCCACACACACCGTACAGCCTCTCGTCGCCCATGCACGGAC
C
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