DY260467

Overview
NameDY260467
Unique NameDY260467
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1301
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig1545 contig Ccv1_Contig1545:1..1301. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: YPTC1_CHLRE (GTP-binding protein YPTC1 OS=Chlamydomonas reinhardtii GN=YPTC1 PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.905e-24
Identity = 59/153 (38.56%), Postives = 97/153 (63.40%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGK+ L+ ++ +  ++  Y +TIG DF  + V+ + ++  LQIWDTAGQERF+++  ++YRGA   ++VYDV   +SF+N+  W  E    AS    +N   +++GNK D+   + +VV    A+A+ A +  IP+ ET  K
Sbjct:    8 LFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTVELDGKVIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLNEIDRYAS----ENVNKLLVGNKSDLT--SKKVVEYSVAKAF-ADEIGIPFLETSAK 153

HSP 2 Score: 22.7126 bits (47), Expect = 1.905e-24
Identity = 13/28 (46.43%), Postives = 16/28 (57.14%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIA---KNALKS 643
            L  SAK   NVE+AF  +A   KN + S
Sbjct:  148 LETSAKNATNVEQAFMTMAAEIKNRMAS 175
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB8B_DICDI (Ras-related protein Rab-8B OS=Dictyostelium discoideum GN=rab8B PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.905e-24
Identity = 60/159 (37.74%), Postives = 95/159 (59.75%), Query Frame = 1 
Query:  109 PSRRRTLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            P+    L+K++++GDSGVGK+ L+ ++ +  F+  + ATIG DF  + ++ E +   LQIWDTAGQERF+++  A+YRGA   +LVYDV   KSF ++ NW       AS    D+   +++GNK D+     +VV   + ++  A +  I + ET  K
Sbjct:    9 PAAYDFLVKLLLIGDSGVGKSCLLLRFSDGSFTPSFIATIGIDFKIRTIELEGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEKSFGSIRNWIRNIEQHAS----DSVNKMLIGNKCDMT--EKKVVDSSRGKS-LADEYGIKFLETSAK 160

HSP 2 Score: 26.5646 bits (57), Expect = 1.905e-24
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKNALK 640
            L  SAK  +NVEEAF  +AK+  K
Sbjct:  155 LETSAKNSVNVEEAFIGLAKDIKK 178
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB8A_CHICK (Ras-related protein Rab-8A OS=Gallus gallus GN=RAB8A PE=2 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.479e-24
Identity = 61/153 (39.87%), Postives = 93/153 (60.78%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT  + ++    F+  + +TIG DF  + ++ + +   LQIWDTAGQERF+++  A+YRGA   +LVYD+ + KSF+N+ NW       ASP    +   ++LGNK D +  + R VS ++     AS G I + ET  K
Sbjct:    8 LFKLLLIGDSGVGKTCALFRFSEDAFNATFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFENIRNWVRNIEEHASP----DVEKMILGNKCDAN--DKRQVSREQGEKLAASFG-IKFMETSAK 153

HSP 2 Score: 22.3274 bits (46), Expect = 2.479e-24
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKN 631
            +  SAK  IN+E AF  +A++
Sbjct:  148 METSAKANINIENAFFTLARD 168
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB1_DISOM (Ras-related protein ORAB-1 OS=Discopyge ommata PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 2.481e-24
Identity = 58/153 (37.91%), Postives = 95/153 (62.09%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGK+ L+ ++ +  ++  Y +TIG DF  + ++ + +   LQIWDTAGQERF+++  ++YRGA   ++VYDV   +SF+N+  W +E    AS    +N   +++GNK D+     +VV    A+ +  S G IP+ ET  K
Sbjct:    8 LFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYAS----ENVNKLLVGNKCDLT--TKKVVDYTTAKEFADSLG-IPFLETSAK 153

HSP 2 Score: 22.7126 bits (47), Expect = 2.481e-24
Identity = 11/24 (45.83%), Postives = 13/24 (54.17%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIAKNALK 640
            L  SAK   NVE+AF  +A    K
Sbjct:  148 LETSAKNATNVEQAFMTMAAEIKK 171
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RIC1_ORYSJ (Ras-related protein RIC1 OS=Oryza sativa subsp. japonica GN=RIC1 PE=2 SV=2)

HSP 1 Score: 111.309 bits (277), Expect = 2.481e-24
Identity = 60/153 (39.22%), Postives = 95/153 (62.09%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGK+ L+ ++ +  +   Y +TIG DF  + V+ + +   LQIWDTAGQERF+++  ++YRGA   ++VYDV   +SF+N+  W  E    AS    +N   +++GNK D+    +RVVS +  +A  A +  IP+ ET  K
Sbjct:    8 LFKLLLIGDSGVGKSCLLLRFADDSYLESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLNEIDRYAS----ENVNKLLVGNKCDL--AENRVVSYEAGKA-LADEIGIPFLETSAK 153

HSP 2 Score: 23.0978 bits (48), Expect = 2.481e-24
Identity = 13/28 (46.43%), Postives = 17/28 (60.71%), Query Frame = 2 
Query:  569 LRPSAKEGINVEEAFQCIA---KNALKS 643
            L  SAK+  NVE+AF  +A   KN + S
Sbjct:  148 LETSAKDATNVEKAFMTMAGEIKNRMAS 175
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB10_PONAB (Ras-related protein Rab-10 OS=Pongo abelii GN=RAB10 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 2.481e-24
Identity = 60/153 (39.22%), Postives = 95/153 (62.09%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++ +  F+  + +TIG DF  K V+ + +   LQIWDTAGQERF ++  ++YRGA   +LVYD+ + KSF+N++ W       A+    ++   ++LGNK D+D  + RVV + K     A +  I +FET  K
Sbjct:    9 LFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHAN----EDVERMLLGNKCDMD--DKRVVPKGKGEQ-IAREHGIRFFETSAK 154

HSP 2 Score: 24.2534 bits (51), Expect = 2.481e-24
Identity = 10/21 (47.62%), Postives = 16/21 (76.19%), Query Frame = 2 
Query:  578 SAKEGINVEEAFQCIAKNALK 640
            SAK  IN+E+AF  +A++ L+
Sbjct:  152 SAKANINIEKAFLTLAEDILR 172
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB10_MOUSE (Ras-related protein Rab-10 OS=Mus musculus GN=Rab10 PE=1 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 2.481e-24
Identity = 60/153 (39.22%), Postives = 95/153 (62.09%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++ +  F+  + +TIG DF  K V+ + +   LQIWDTAGQERF ++  ++YRGA   +LVYD+ + KSF+N++ W       A+    ++   ++LGNK D+D  + RVV + K     A +  I +FET  K
Sbjct:    9 LFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHAN----EDVERMLLGNKCDMD--DKRVVPKGKGEQ-IAREHGIRFFETSAK 154

HSP 2 Score: 24.2534 bits (51), Expect = 2.481e-24
Identity = 10/21 (47.62%), Postives = 16/21 (76.19%), Query Frame = 2 
Query:  578 SAKEGINVEEAFQCIAKNALK 640
            SAK  IN+E+AF  +A++ L+
Sbjct:  152 SAKANINIEKAFLTLAEDILR 172
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB10_HUMAN (Ras-related protein Rab-10 OS=Homo sapiens GN=RAB10 PE=1 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 2.481e-24
Identity = 60/153 (39.22%), Postives = 95/153 (62.09%), Query Frame = 1 
Query:  127 LLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            L K++++GDSGVGKT ++ ++ +  F+  + +TIG DF  K V+ + +   LQIWDTAGQERF ++  ++YRGA   +LVYD+ + KSF+N++ W       A+    ++   ++LGNK D+D  + RVV + K     A +  I +FET  K
Sbjct:    9 LFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHAN----EDVERMLLGNKCDMD--DKRVVPKGKGEQ-IAREHGIRFFETSAK 154

HSP 2 Score: 24.2534 bits (51), Expect = 2.481e-24
Identity = 10/21 (47.62%), Postives = 16/21 (76.19%), Query Frame = 2 
Query:  578 SAKEGINVEEAFQCIAKNALK 640
            SAK  IN+E+AF  +A++ L+
Sbjct:  152 SAKANINIEKAFLTLAEDILR 172
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB5B_PONAB (Ras-related protein Rab-5B OS=Pongo abelii GN=RAB5B PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 3.222e-24
Identity = 59/151 (39.07%), Postives = 92/151 (60.93%), Query Frame = 1 
Query:  133 KVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            K+++LG+S VGK+SL+ ++V  +F    ++TIGA FLT+ V  +D     +IWDTAGQER+ SL   +YRGA   ++VYD+ + ++F     W +E   QASPS        + GNK D+   N R+V  ++A+A+ A   ++ + ET  K
Sbjct:   22 KLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPS----IVIALAGNKADL--ANKRMVEYEEAQAY-ADDNSLLFMETSAK 165

HSP 2 Score: 23.483 bits (49), Expect = 3.222e-24
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  563 LTLRPSAKEGINVEEAFQCIAKNALKS 643
            L +  SAK  +NV + F  IAK   KS
Sbjct:  158 LFMETSAKTAMNVNDLFLAIAKKLPKS 184
[Close]
BLAST of DY260467 vs. ExPASy Swiss-Prot
Match: RAB5B_MOUSE (Ras-related protein Rab-5B OS=Mus musculus GN=Rab5b PE=1 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 3.222e-24
Identity = 59/151 (39.07%), Postives = 92/151 (60.93%), Query Frame = 1 
Query:  133 KVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQASPSDPDNFPFVVLGNKIDVDGGNSRVVSEKKARAWCASKGNIPYFETLCK 585
            K+++LG+S VGK+SL+ ++V  +F    ++TIGA FLT+ V  +D     +IWDTAGQER+ SL   +YRGA   ++VYD+ + ++F     W +E   QASPS        + GNK D+   N R+V  ++A+A+ A   ++ + ET  K
Sbjct:   22 KLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPS----IVIALAGNKADL--ANKRMVEYEEAQAY-ADDNSLLFMETSAK 165

HSP 2 Score: 23.483 bits (49), Expect = 3.222e-24
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  563 LTLRPSAKEGINVEEAFQCIAKNALKS 643
            L +  SAK  +NV + F  IAK   KS
Sbjct:  158 LFMETSAKTAMNVNDLFLAIAKKLPKS 184
The following BLAST results are available for this feature:
BLAST of DY260467 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
YPTC1_CHLRE1.905e-2438.56GTP-binding protein YPTC1 OS=Chlamydomonas reinhar... [more]
RAB8B_DICDI1.905e-2437.74Ras-related protein Rab-8B OS=Dictyostelium discoi... [more]
RAB8A_CHICK2.479e-2439.87Ras-related protein Rab-8A OS=Gallus gallus GN=RAB... [more]
RAB1_DISOM2.481e-2437.91Ras-related protein ORAB-1 OS=Discopyge ommata PE=... [more]
RIC1_ORYSJ2.481e-2439.22Ras-related protein RIC1 OS=Oryza sativa subsp. ja... [more]
RAB10_PONAB2.481e-2439.22Ras-related protein Rab-10 OS=Pongo abelii GN=RAB1... [more]
RAB10_MOUSE2.481e-2439.22Ras-related protein Rab-10 OS=Mus musculus GN=Rab1... [more]
RAB10_HUMAN2.481e-2439.22Ras-related protein Rab-10 OS=Homo sapiens GN=RAB1... [more]
RAB5B_PONAB3.222e-2439.07Ras-related protein Rab-5B OS=Pongo abelii GN=RAB5... [more]
RAB5B_MOUSE3.222e-2439.07Ras-related protein Rab-5B OS=Mus musculus GN=Rab5... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP12YJ07FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY260467 ID=DY260467; Name=DY260467; organism=Citrus clementina; type=EST; length=1301bp
CGCACGCAGAGCCTCTTTAACAACTACGGAGCACGTCAAAACGATCAACT
GATTTGCGTTTGGAGGTAGATCGTTCGATCGATTGACCGATTTTGCCCCA
GAACGATGCCGTCTCGTAGAAGAACTCTCCTCAAGGTCATCATCCTCGGC
GACAGCGGGGTGGGCAAGACCTCTTTGATGAATCAATATGTAAATAAGAA
GTTTAGCAATCAGTACAAGGCGACCATCGGAGCTGATTTCTTGACAAAGG
AAGTGCAGTTTGAAGATAGGCTCTTCACCTTACAGATCTGGGATACAGCT
GGTCAGGAAAGATTCCAAAGCCTGGGTGTTGCTTTCTACCGAGGTGCAGA
TTGCTGTGTACTTGTGTATGATGTAAATTCTATGAAGTCCTTTGACAACC
TCAATAACTGGAGAGAAGAATTCCTCATTCAGGCTAGCCCTTCGGATCCA
GACAATTTCCCCTTTGTTGTTTTAGGAAACAAGATTGATGTAGATGGTGG
AAACAGTAGGGTGGTTTCGGAGAAGAAGGCTCGAGCCTGGTGTGCCTCCA
AAGGAAATATTCCTTACTTTGAGACCCTCTGCAAAAGAAGGCATTAATGT
GGAAGAAGCTTTCCAGTGCATAGCTAAAAATGCTTTGAAGAGTGGGGAGG
AGGAACAGATACACCTTGCCGGACACCATTGATGTTGGAACCCGCCCCCC
GCCCAGGGCCAGTGGAAGCAAGTGCCAAAGATATGGGCCAATTTTCTTCA
AGGACCCTCAACCACTTACAGAAATTGATTGGCCCTTTATAACATTTTTC
ATGGGGAACGCAAAGGGTTGCGGTGCAATCTAATGCTTGGGGCCAACCAA
CCCGGTCCCTCCCACCCACCTCCCCTCATCTGGTCCCTGGACTCGCCCCT
CAACCCCACCCATCCACTCGTACACTGCCTCCCCCCCCCCGCACGCCATC
CTGACTCTCCCCTATCCCGCGCAACCCGCCCGTTGGTCCTCACCCCCCTC
TATGCATCTCCCCCACCAGGACCAGCCATACAAGATCCACAGGCACAACA
CTTCCCTCTCTACACATCACTCTGCCCCTTCATGCCCAACCACTACGACC
TACATCACAACTCCCATCCACGACACAACCACCACATCCCCCCCCCCACC
ACACACCCCGACTCACTACTCCAGACACCTCCCCCCGCTGCACTCCTCCA
CAGACCCAGGCCCTGCATACCACACGCCGCCTGAACACACCACACCCCAA
CACTACATGCCCACACACACCGTACAGCCTCTCGTCGCCCATGCACGGAC
C
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