A new strategy employed for identification of sweet orange cultivars with RAPD markers

Publication Overview
TitleA new strategy employed for identification of sweet orange cultivars with RAPD markers
AuthorsSun X, Mu Q, Jiang D, Wang C, Wang XC, Fang JG
TypeJournal Article
Journal NameGenetics and molecular research : GMR
Volume11
Issue3
Year2012
Page(s)2071-80
CitationSun X, Mu Q, Jiang D, Wang C, Wang XC, Fang JG. A new strategy employed for identification of sweet orange cultivars with RAPD markers. Genetics and molecular research : GMR. 2012; 11(3):2071-80.

Abstract

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification. Thirteen effective primers were first screened from 54 RAPD arbitrary 11-mer primers based on their amplification products and amplified polymorphic bands; they were then used for PCR amplification of all 60 cultivars. All cultivars were manually and completely separated by the polymorphic bands appearing in DNA fingerprints from 13 primers; a CID of the 60 sweet orange cultivars was then constructed. This CID separated all the cultivars from each other, based on the polymorphic bands; the corresponding primers were marked in the correct positions on the sweet orange CID. The CID strategy facilitates the identification of fruit cultivars with DNA markers. This CID of sweet orange cultivars will be very useful for the protection of cultivar rights and for early identification of seedlings in the nursery industry.

Stocks
This publication contains information about 59 stocks:
Stock NameUniquenameType
Anjiangdahongcheng 1haoAnjiangdahongcheng 1haoaccession
Anyuan C3Anyuan C3accession
Cadenera Sweet OrangeCadenera Sweet Orangeaccession
Cara red flesh navel orangeCara red flesh navel orangeaccession
Chengxi late-maturingChengxi late-maturingaccession
Cuba taoyechengCuba taoyechengaccession
DeltaDeltaaccession
Egypt honey orangeEgypt honey orangeaccession
Eight lanes tianchengEight lanes tianchengaccession
FengqiFengqiaccession
Fukumoto Navel OrangeFukumoto Navel Orangeaccession
GuangxixiangshuichengGuangxixiangshuichengaccession
HamlinHamlinaccession
Hanyuan seedlessHanyuan seedlessaccession
HongmaochengHongmaochengaccession
HuangbaipitianchengHuangbaipitianchengaccession
Huapeng 9-11Huapeng 9-11accession
Ichang papedaIchang papedaaccession
Italy hamlinItaly hamlinaccession
JiangbeimeiganJiangbeimeiganaccession
JintangchengJintangchengaccession
Kaixian seedlessKaixian seedlessaccession
KaixianbopiKaixianbopiaccession
Late-maturing navel orangeLate-maturing navel orangeaccession
LingyangshaohedahongtianchengLingyangshaohedahongtianchengaccession

Pages

Features
This publication contains information about 13 features:
Feature NameUniquenameType
Y10Y10genetic_marker
Y15Y15genetic_marker
Y17Y17genetic_marker
Y18Y18genetic_marker
Y24Y24genetic_marker
Y28Y28genetic_marker
Y34Y34genetic_marker
Y35Y35genetic_marker
Y36Y36genetic_marker
Y41Y41genetic_marker
Y46Y46genetic_marker
Y47Y47genetic_marker
Y57Y57genetic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic
ISSN1676-5680
eISSN1676-5680
Publication Date2012
Journal AbbreviationGenet. Mol. Res.
DOI10.4238/2012.August.6.11
Elocation10.4238/2012.August.6.11
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryBrazil
Cross References
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DatabaseAccession
PMID: PubMedPMID:22911591