A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers

Publication Overview
TitleA new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers
AuthorsGulsen O, Uzun A, Canan I, Seday U, Canihos E
TypeJournal Article
Journal NameEuphytica
Volume173
Issue2
Year2010
Page(s)265-277
CitationGulsen O, Uzun A, Canan I, Seday U, Canihos E. A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica. 2010; 173(2):265-277.

Abstract

Sequence-related amplified polymorphism (SRAP), simple sequence repeats (SSR), inter-simple sequence repeat (ISSR), peroxidase gene polymorphism (POGP), resistant gene analog (RGA), randomly amplified polymorphic DNA (RAPD), and a morphological marker, Alternaria brown spot resistance gene of citrus named as Cabsr caused by (Alternaria alternata f. sp. Citri) were used to establish genetic linkage map of citrus using a population of 164 F₁ individuals derived between ‘Clementine' mandarin (Citrus reticulata Blanco ‘Clementine) and ‘Orlando' tangelo' (C. paradisi Macf. ‘Duncan' × C. reticulata Blanco ‘Dancy'). A total of 609 markers, including 385 SRAP, 97 RAPD, 95 SSR, 18 ISSR, 12 POGP, and 2 RGA markers were used in linkage analysis. The ‘Clementine' linkage map has 215 markers, comprising 144 testcross and 71 intercross markers placed in nine linkage groups. The ‘Clementine' linkage map covered 858 cM with and average map distance of 3.5 cM between adjacent markers. The ‘Orlando' linkage map has 189 markers, comprising 126 testcross and 61 intercross markers placed in nine linkage groups. The ‘Orlando' linkage map covered 886 cM with an average map distance of 3.9 cM between adjacent markers. Segregation ratios for Cabsr were not significantly different from 1:1, suggesting that this trait is controlled by a single locus. This locus was placed in ‘Orlando' linkage group 1. The new map has an improved distribution of markers along the linkage groups with fewer gaps. Combining different marker systems in linkage mapping studies may give better genome coverage due to their chromosomal target site differences, therefore fewer gaps in linkage groups.
Features
This publication contains information about 614 features:
Feature NameUniquenameType
SI137SI137genetic_marker
SI138SI138genetic_marker
SI139SI139genetic_marker
SI140SI140genetic_marker
SI141SI141genetic_marker
SI142SI142genetic_marker
SI143SI143genetic_marker
SI144SI144genetic_marker
SI145SI145genetic_marker
SI146SI146genetic_marker
SI148SI148genetic_marker
SI149SI149genetic_marker
SI150SI150genetic_marker
SI151SI151genetic_marker
SI152SI152genetic_marker
SI153SI153genetic_marker
SI154SI154genetic_marker
SI155SI155genetic_marker
SI156SI156genetic_marker
SI158SI158genetic_marker
SI161SI161genetic_marker
SI163SI163genetic_marker
SI166SI166genetic_marker
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SI169SI169genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-Orlando/Clementine-F1-Clementine-2010
Citrus-Orlando/Clementine-F1-Orlando-2010
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
DancyDancyaccession
DuncanDuncanaccession
OrlandoOrlandoaccession
ClementineClementineaccession
Citrus-Orlando/Clementine-F1-ClementineCitrus-Orlando/Clementine-F1-Clementinepopulation
Citrus-Orlando/Clementine-F1-OrlandoCitrus-Orlando/Clementine-F1-Orlandopopulation
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2010
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s10681-010-0146-7
KeywordsCitrus reticulata, clementines, genetic markers, microsatellite repeats, genetic polymorphism, peroxidase, random amplified polymorphic DNA technique, Alternaria alternata, plant pathogenic fungi, fungal diseases of plants, disease resistance, genetic resistance, linkage (genetics), chromosome mapping, Citrus paradisi, tangelos, interspecific hybridization, linkage groups, gene segregation, genome, sequence related amplified polymorphism, resistance genes, resistant gene analog, molecular sequence data