A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers

Publication Overview
TitleA new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers
AuthorsGulsen O, Uzun A, Canan I, Seday U, Canihos E
TypeJournal Article
Journal NameEuphytica
Volume173
Issue2
Year2010
Page(s)265-277
CitationGulsen O, Uzun A, Canan I, Seday U, Canihos E. A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica. 2010; 173(2):265-277.

Abstract

Sequence-related amplified polymorphism (SRAP), simple sequence repeats (SSR), inter-simple sequence repeat (ISSR), peroxidase gene polymorphism (POGP), resistant gene analog (RGA), randomly amplified polymorphic DNA (RAPD), and a morphological marker, Alternaria brown spot resistance gene of citrus named as Cabsr caused by (Alternaria alternata f. sp. Citri) were used to establish genetic linkage map of citrus using a population of 164 F₁ individuals derived between ‘Clementine' mandarin (Citrus reticulata Blanco ‘Clementine) and ‘Orlando' tangelo' (C. paradisi Macf. ‘Duncan' × C. reticulata Blanco ‘Dancy'). A total of 609 markers, including 385 SRAP, 97 RAPD, 95 SSR, 18 ISSR, 12 POGP, and 2 RGA markers were used in linkage analysis. The ‘Clementine' linkage map has 215 markers, comprising 144 testcross and 71 intercross markers placed in nine linkage groups. The ‘Clementine' linkage map covered 858 cM with and average map distance of 3.5 cM between adjacent markers. The ‘Orlando' linkage map has 189 markers, comprising 126 testcross and 61 intercross markers placed in nine linkage groups. The ‘Orlando' linkage map covered 886 cM with an average map distance of 3.9 cM between adjacent markers. Segregation ratios for Cabsr were not significantly different from 1:1, suggesting that this trait is controlled by a single locus. This locus was placed in ‘Orlando' linkage group 1. The new map has an improved distribution of markers along the linkage groups with fewer gaps. Combining different marker systems in linkage mapping studies may give better genome coverage due to their chromosomal target site differences, therefore fewer gaps in linkage groups.
Features
This publication contains information about 614 features:
Feature NameUniquenameType
Em6Me8aEm6Me8agenetic_marker
Em6Me8bEm6Me8bgenetic_marker
Em7Me13aEm7Me13agenetic_marker
Em7Me13bEm7Me13bgenetic_marker
Em7Me13cEm7Me13cgenetic_marker
Em7Me2aEm7Me2agenetic_marker
Em7Me3aEm7Me3agenetic_marker
Em7Me5aEm7Me5agenetic_marker
Em7Me5bEm7Me5bgenetic_marker
Em7Me6aEm7Me6agenetic_marker
Em7Me6bEm7Me6bgenetic_marker
Em7Me8aEm7Me8agenetic_marker
Em7Me8cEm7Me8cgenetic_marker
Em7Me9aEm7Me9agenetic_marker
Em7Me9bEm7Me9bgenetic_marker
Em8Me11bEm8Me11bgenetic_marker
Em8Me11dEm8Me11dgenetic_marker
Em8Me12aEm8Me12agenetic_marker
Em8Me12bEm8Me12bgenetic_marker
Em8Me3aEm8Me3agenetic_marker
Em8Me3bEm8Me3bgenetic_marker
Em8Me5aEm8Me5agenetic_marker
Em9Me11aEm9Me11agenetic_marker
Em9Me11bEm9Me11bgenetic_marker
Em9Me11cEm9Me11cgenetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-Orlando/Clementine-F1-Clementine-2010
Citrus-Orlando/Clementine-F1-Orlando-2010
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
DancyDancyaccession
DuncanDuncanaccession
OrlandoOrlandoaccession
ClementineClementineaccession
Citrus-Orlando/Clementine-F1-ClementineCitrus-Orlando/Clementine-F1-Clementinepopulation
Citrus-Orlando/Clementine-F1-OrlandoCitrus-Orlando/Clementine-F1-Orlandopopulation
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2010
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s10681-010-0146-7
KeywordsCitrus reticulata, clementines, genetic markers, microsatellite repeats, genetic polymorphism, peroxidase, random amplified polymorphic DNA technique, Alternaria alternata, plant pathogenic fungi, fungal diseases of plants, disease resistance, genetic resistance, linkage (genetics), chromosome mapping, Citrus paradisi, tangelos, interspecific hybridization, linkage groups, gene segregation, genome, sequence related amplified polymorphism, resistance genes, resistant gene analog, molecular sequence data