A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers

Publication Overview
TitleA new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers
AuthorsGulsen O, Uzun A, Canan I, Seday U, Canihos E
TypeJournal Article
Journal NameEuphytica
Volume173
Issue2
Year2010
Page(s)265-277
CitationGulsen O, Uzun A, Canan I, Seday U, Canihos E. A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica. 2010; 173(2):265-277.

Abstract

Sequence-related amplified polymorphism (SRAP), simple sequence repeats (SSR), inter-simple sequence repeat (ISSR), peroxidase gene polymorphism (POGP), resistant gene analog (RGA), randomly amplified polymorphic DNA (RAPD), and a morphological marker, Alternaria brown spot resistance gene of citrus named as Cabsr caused by (Alternaria alternata f. sp. Citri) were used to establish genetic linkage map of citrus using a population of 164 F₁ individuals derived between ‘Clementine' mandarin (Citrus reticulata Blanco ‘Clementine) and ‘Orlando' tangelo' (C. paradisi Macf. ‘Duncan' × C. reticulata Blanco ‘Dancy'). A total of 609 markers, including 385 SRAP, 97 RAPD, 95 SSR, 18 ISSR, 12 POGP, and 2 RGA markers were used in linkage analysis. The ‘Clementine' linkage map has 215 markers, comprising 144 testcross and 71 intercross markers placed in nine linkage groups. The ‘Clementine' linkage map covered 858 cM with and average map distance of 3.5 cM between adjacent markers. The ‘Orlando' linkage map has 189 markers, comprising 126 testcross and 61 intercross markers placed in nine linkage groups. The ‘Orlando' linkage map covered 886 cM with an average map distance of 3.9 cM between adjacent markers. Segregation ratios for Cabsr were not significantly different from 1:1, suggesting that this trait is controlled by a single locus. This locus was placed in ‘Orlando' linkage group 1. The new map has an improved distribution of markers along the linkage groups with fewer gaps. Combining different marker systems in linkage mapping studies may give better genome coverage due to their chromosomal target site differences, therefore fewer gaps in linkage groups.
Features
This publication contains information about 614 features:
Feature NameUniquenameType
Em5Me11cEm5Me11cgenetic_marker
Em5Me11dEm5Me11dgenetic_marker
Em5Me12aEm5Me12agenetic_marker
Em5Me2aEm5Me2agenetic_marker
Em5Me2bEm5Me2bgenetic_marker
Em5Me3aEm5Me3agenetic_marker
Em5ME3bEm5ME3bgenetic_marker
Em5Me5eEm5Me5egenetic_marker
Em5Me6aEm5Me6agenetic_marker
Em5Me8bEm5Me8bgenetic_marker
Em5Me9aEm5Me9agenetic_marker
Em6Me11aEm6Me11agenetic_marker
Em6Me11bEm6Me11bgenetic_marker
Em6Me11cEm6Me11cgenetic_marker
Em6Me12cEm6Me12cgenetic_marker
Em6Me12dEm6Me12dgenetic_marker
Em6Me12eEm6Me12egenetic_marker
Em6Me12fEm6Me12fgenetic_marker
Em6Me1aEm6Me1agenetic_marker
Em6Me1dEm6Me1dgenetic_marker
Em6Me1eEm6Me1egenetic_marker
Em6Me2aEm6Me2agenetic_marker
Em6Me7aEm6Me7agenetic_marker
Em6Me7bEm6Me7bgenetic_marker
Em6Me7dEm6Me7dgenetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-Orlando/Clementine-F1-Clementine-2010
Citrus-Orlando/Clementine-F1-Orlando-2010
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
DancyDancyaccession
DuncanDuncanaccession
OrlandoOrlandoaccession
ClementineClementineaccession
Citrus-Orlando/Clementine-F1-ClementineCitrus-Orlando/Clementine-F1-Clementinepopulation
Citrus-Orlando/Clementine-F1-OrlandoCitrus-Orlando/Clementine-F1-Orlandopopulation
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2010
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s10681-010-0146-7
KeywordsCitrus reticulata, clementines, genetic markers, microsatellite repeats, genetic polymorphism, peroxidase, random amplified polymorphic DNA technique, Alternaria alternata, plant pathogenic fungi, fungal diseases of plants, disease resistance, genetic resistance, linkage (genetics), chromosome mapping, Citrus paradisi, tangelos, interspecific hybridization, linkage groups, gene segregation, genome, sequence related amplified polymorphism, resistance genes, resistant gene analog, molecular sequence data