DY270893

Overview
NameDY270893
Unique NameDY270893
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length1247
Libraries
Library NameType
Fruit-TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS2_CITSI (Chalcone synthase 2 OS=Citrus sinensis GN=CHS2 PE=2 SV=1)

HSP 1 Score: 350.132 bits (897), Expect = 2.253e-98
Identity = 173/175 (98.86%), Postives = 173/175 (98.86%), Query Frame = 2 
Query:   32 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQ CFAGG VLRLAK
Sbjct:    1 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAK 175

HSP 2 Score: 31.9574 bits (71), Expect = 2.253e-98
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENNKGA V VVCS I+
Sbjct:  175 KDLAENNKGARVLVVCSEIT 194
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS3_RUTGR (Chalcone synthase 3 OS=Ruta graveolens GN=CHS3 PE=2 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 3.249e-91
Identity = 160/173 (92.49%), Postives = 166/173 (95.95%), Query Frame = 2 
Query:   38 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            TV+EIR AQRADGPATVLAIGTATPA+ V QADYPDYYFRITKSEHMT+LKEKFKRMCDKSMI+KRYM+LTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPG DYQLTKL+GLRPSVKRFMMYQQ CFAGG VLRLAK
Sbjct:    5 TVEEIRKAQRADGPATVLAIGTATPANYVTQADYPDYYFRITKSEHMTDLKEKFKRMCDKSMIRKRYMHLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAAKAIKEWGQPKSKITHLIFCTTSGVDMPGCDYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAK 177

HSP 2 Score: 30.8018 bits (68), Expect = 3.249e-91
Identity = 14/20 (70.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENN+GA V VVCS I+
Sbjct:  177 KDLAENNRGARVLVVCSEIT 196
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS2_RUTGR (Chalcone synthase 2 OS=Ruta graveolens GN=CHS2 PE=2 SV=1)

HSP 1 Score: 327.405 bits (838), Expect = 3.249e-91
Identity = 160/173 (92.49%), Postives = 165/173 (95.38%), Query Frame = 2 
Query:   38 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            TV+EIR AQRADGPA VLAIGTATPA+ V QADYPDYYFRITKSEHMTELKEKFKRMCDKSMI+KRYMYLTE+ILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPG DYQLTKL+GLRPSVKRFMMYQQ CFAGG VLRLAK
Sbjct:    5 TVEEIRKAQRADGPAAVLAIGTATPANYVTQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIRKRYMYLTEDILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGCDYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAK 177

HSP 2 Score: 30.8018 bits (68), Expect = 3.249e-91
Identity = 14/20 (70.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENN+GA V VVCS I+
Sbjct:  177 KDLAENNRGARVLVVCSEIT 196
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHSY_BETVE (Chalcone synthase OS=Betula verrucosa GN=CHS PE=2 SV=2)

HSP 1 Score: 322.783 bits (826), Expect = 3.554e-90
Identity = 157/175 (89.71%), Postives = 165/175 (94.29%), Query Frame = 2 
Query:   32 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            MA+V+EIR AQRA GPATVLAIGTATP++ + QADYPDYYFRITKS+HMTELKEKFKRMCDKSMIKKRYMYL EEIL ENPNMCAYMAPSLDARQ IVVVEVPKLGKEAATKAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQ CFAGG VLRLAK
Sbjct:    1 MASVEEIRKAQRAHGPATVLAIGTATPSNCITQADYPDYYFRITKSDHMTELKEKFKRMCDKSMIKKRYMYLNEEILNENPNMCAYMAPSLDARQTIVVVEVPKLGKEAATKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAK 175

HSP 2 Score: 31.9574 bits (71), Expect = 3.554e-90
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENNKGA V VVCS I+
Sbjct:  175 KDLAENNKGARVLVVCSEIT 194
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS1_RUTGR (Chalcone synthase 1 OS=Ruta graveolens GN=CHS1 PE=2 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 6.049e-90
Identity = 158/173 (91.33%), Postives = 164/173 (94.80%), Query Frame = 2 
Query:   38 TVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            TV+ IR AQRADGPA VLAIGTATPA+ V QADYPDYYFRITKSEHMTELKEKFKRMCDKSMI+KRYM+LTE+ILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPG DYQLTKL+GLRPSVKRFMMYQQ CFAGG VLRLAK
Sbjct:    5 TVEAIRKAQRADGPAAVLAIGTATPANYVTQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIRKRYMHLTEDILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGCDYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAK 177

HSP 2 Score: 30.8018 bits (68), Expect = 6.049e-90
Identity = 14/20 (70.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENN+GA V VVCS I+
Sbjct:  177 KDLAENNRGARVLVVCSEIT 196
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS3_SOYBN (Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 1.343e-89
Identity = 155/175 (88.57%), Postives = 167/175 (95.43%), Query Frame = 2 
Query:   32 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            M +V+EIRNAQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQ CFAGG VLRLAK
Sbjct:    1 MVSVEEIRNAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAK 175

HSP 2 Score: 31.9574 bits (71), Expect = 1.343e-89
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENNKGA V VVCS I+
Sbjct:  175 KDLAENNKGARVLVVCSEIT 194
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS1_SOYBN (Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV=1)

HSP 1 Score: 318.546 bits (815), Expect = 6.618e-89
Identity = 154/175 (88.00%), Postives = 166/175 (94.86%), Query Frame = 2 
Query:   32 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            M +V+EIR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQ CFAGG VLRLAK
Sbjct:    1 MVSVEEIRKAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAK 175

HSP 2 Score: 31.9574 bits (71), Expect = 6.618e-89
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENNKGA V VVCS I+
Sbjct:  175 KDLAENNKGARVLVVCSEIT 194
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS2_CAMSI (Chalcone synthase 2 OS=Camellia sinensis GN=CHS2 PE=2 SV=1)

HSP 1 Score: 318.161 bits (814), Expect = 8.632e-89
Identity = 153/175 (87.43%), Postives = 165/175 (94.29%), Query Frame = 2 
Query:   32 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            M TV+E+R AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEH TELKEKF+RMCDKSMIKKRYMYLTEEILKENPN+CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQ CFAGG VLRLAK
Sbjct:    1 MVTVEEVRRAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHKTELKEKFQRMCDKSMIKKRYMYLTEEILKENPNVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAK 175

HSP 2 Score: 31.9574 bits (71), Expect = 8.632e-89
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENNKGA V VVCS I+
Sbjct:  175 KDLAENNKGARVLVVCSEIT 194
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS5_SOYBN (Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV=1)

HSP 1 Score: 317.39 bits (812), Expect = 1.469e-88
Identity = 153/175 (87.43%), Postives = 166/175 (94.86%), Query Frame = 2 
Query:   32 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            M +V+EIR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VV+EVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQ CFAGG VLRLAK
Sbjct:    1 MVSVEEIRQAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAK 175

HSP 2 Score: 31.9574 bits (71), Expect = 1.469e-88
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENNKGA V VVCS I+
Sbjct:  175 KDLAENNKGARVLVVCSEIT 194
[Close]
BLAST of DY270893 vs. ExPASy Swiss-Prot
Match: CHS2_SOYBN (Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV=1)

HSP 1 Score: 317.39 bits (812), Expect = 1.469e-88
Identity = 153/175 (87.43%), Postives = 166/175 (94.86%), Query Frame = 2 
Query:   32 MATVQEIRNAQRADGPATVLAIGTATPAHSVNQADYPDYYFRITKSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQRCFAGGIVLRLAK 556
            M +V+EIR AQRA+GPATV+AIGTATP + V+Q+ YPDYYFRIT SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VV+EVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQ CFAGG VLRLAK
Sbjct:    1 MVSVEEIRKAQRAEGPATVMAIGTATPPNCVDQSTYPDYYFRITNSEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAK 175

HSP 2 Score: 31.9574 bits (71), Expect = 1.469e-88
Identity = 15/20 (75.00%), Postives = 17/20 (85.00%), Query Frame = 3 
Query:  555 KDVAENNKGAHVHVVCS*IS 614
            KD+AENNKGA V VVCS I+
Sbjct:  175 KDLAENNKGARVLVVCSEIT 194
The following BLAST results are available for this feature:
BLAST of DY270893 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 144
Match NameE-valueIdentityDescription
CHS2_CITSI2.253e-9898.86Chalcone synthase 2 OS=Citrus sinensis GN=CHS2 PE=... [more]
CHS3_RUTGR3.249e-9192.49Chalcone synthase 3 OS=Ruta graveolens GN=CHS3 PE=... [more]
CHS2_RUTGR3.249e-9192.49Chalcone synthase 2 OS=Ruta graveolens GN=CHS2 PE=... [more]
CHSY_BETVE3.554e-9089.71Chalcone synthase OS=Betula verrucosa GN=CHS PE=2 ... [more]
CHS1_RUTGR6.049e-9091.33Chalcone synthase 1 OS=Ruta graveolens GN=CHS1 PE=... [more]
CHS3_SOYBN1.343e-8988.57Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV... [more]
CHS1_SOYBN6.618e-8988.00Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV... [more]
CHS2_CAMSI8.632e-8987.43Chalcone synthase 2 OS=Camellia sinensis GN=CHS2 P... [more]
CHS5_SOYBN1.469e-8887.43Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV... [more]
CHS2_SOYBN1.469e-8887.43Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP8YB20FM1 Fruit-TF Citrus clementina cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY270893 ID=DY270893; Name=DY270893; organism=Citrus clementina; type=EST; length=1247bp
CAAACATATTCATCTTTGGGTTCAACTTGAAATGGCAACCGTTCAAGAGA
TCAGAAACGCTCAGCGTGCCGACGGCCCGGCCACCGTCCTCGCCATCGGT
ACGGCCACGCCTGCCCACAGTGTCAACCAGGCTGATTATCCCGACTATTA
CTTCAGGATCACAAAGAGCGAGCATATGACGGAGCTTAAAGAGAAGTTCA
AGCGCATGTGTGACAAGTCGATGATTAAGAAGCGTTACATGTACTTAACG
GAAGAGATTTTGAAGGAAAACCCCAACATGTGCGCTTACATGGCTCCATC
ACTCGACGCACGTCAAGACATTGTGGTGGTCGAAGTGCCGAAGCTCGGGA
AAGAAGCTGCTACAAAGGCCATCAAAGAATGGGGCCAGCCCAAGTCTAAG
ATCACCCACCTCATCTTCTGCACCACCTCCGGCGTCGACATGCCCGGCGC
CGACTACCAGCTCACCAAACTGATCGGCCTGCGCCCCTCCGTCAAGCGCT
TCATGATGTACCAACAAAGATGTTTCGCCGGTGGCATTGTTCTTCGCCTC
GCAAAAAGACGTGGCAGAGAACAACAAGGGCGCTCACGTTCATGTTGTCT
GTTCATAGATCTCTAGGTGACTCGTTCCCGCAGCGCTACGGATATCCTTC
CCTTATCCTCTCGCGCGCCCGAGCTCTTGCTCATTATCTACCCAAGAAAC
TGCATTGATCCGATGCTTTTTACTATAACCATGATTGATTCCATAATTAA
TTTTCTTCTTATCGCTTTTCGCCCTTCTATTAGTTTTTATTTCTTTCTTA
TTCTTTGTCTTTTTTCTTTCTTCATCTTATTTGTTTCTCTTTTAAGTATA
ATATACCAACATCATTATGTACTAATACCTATGGAGCATCTGTACATTTA
TGAGGTTTCGCTGATTACTACTTTTAACAATTATTTTCTTACTTATCATT
TTTTTTATTTGTTTTTGTCTTTTGCTCATGTTCTAAATTCTTTTTGCATG
TATCTAATTTTTTCAATCTTTCTTACTTCTTACTTTATCTGGCTTATCTA
ATCTGATTTTTACTATTGTATTATTGGTCACTGTCTCCACTGTACATATC
AGCTAAATACTGAAGCGCGATATGTACAGCATTTTGGTTTTCTATACGCG
TTGCAATACAAGATATTGTTGATCCAATTGTACTAAAACGGTGGTGTTTA
TGTTATGATATTTGTTCTCTGGTCAAAATAGATAGAATTACAGTATG
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