Assessing the Biosynthetic Capabilities of Secretory Glands in Citrus Peel

Publication Overview
TitleAssessing the Biosynthetic Capabilities of Secretory Glands in Citrus Peel
AuthorsVoo SS, Grimes HD, Lange BM
TypeJournal Article
Journal NamePlant physiology
Volume159
Issue1
Year2012
Page(s)81-94
CitationVoo SS, Grimes HD, Lange BM. Assessing the Biosynthetic Capabilities of Secretory Glands in Citrus Peel. Plant physiology. 2012; 159(1):81-94.

Abstract

Epithelial cells (ECs) lining the secretory cavities of Citrus peel have been hypothesized to be responsible for the synthesis of essential oil, but direct evidence for such a role is currently sparse. We used laser-capture microdissection and pressure catapulting to isolate ECs and parenchyma cells (as controls not synthesizing oil) from the peel of young grapefruit (Citrus x paradisi ‘Duncan’), isolated RNA, and evaluated transcript patterns based on oligonucleotide microarrays. A Gene Ontology analysis of these data sets indicated an enrichment of genes involved in the biosynthesis of volatile terpenoids and nonvolatile phenylpropanoids in ECs (when compared with parenchyma cells), thus indicating a significant metabolic specialization in this cell type. The gene expression patterns in ECs were consistent with the accumulation of the major essential oil constituents (monoterpenes, prenylated coumarins, and polymethoxylated flavonoids). Morphometric analyses demonstrated that secretory cavities are formed early during fruit development, whereas the expansion of cavities, and thus oil accumulation, correlates with later stages of fruit expansion. Our studies have laid the methodological and experimental groundwork for a vastly improved knowledge of the as yet poorly understood processes controlling essential oil biosynthesis in Citrus peel.
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Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2012
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1104/pp.112.194233
KeywordsCitrus paradisi, RNA, biosynthesis, citrus peels, coumarins, epithelial cells, essential oils, flavonoids, fruiting, fruits, gene expression, genes, grapefruits, microarray technology, monoterpenoids, oils, parenchyma, phenylpropanoids
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4702017
PMID: PubMedPMID:22452856