EY832023

Overview
NameEY832023
Unique NameEY832023
TypeEST
OrganismCitrus trifoliata ()
Sequence length854
Libraries
Library NameType
Poncirus trifoliata bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: EKIA_DICDI (Probable ethanolamine kinase A OS=Dictyostelium discoideum GN=etnkA PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.097e-26
Identity = 53/100 (53.00%), Postives = 69/100 (69.00%), Query Frame = 2 
Query:  374 MEQELAGHLNAPVVFSHNDLLSGNIMVNDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGYDCDYSLYPNKDEQNHFFRHYLRP-DKPEEVSDKILKFFM 670
            +EQ LA  LN+P+VF HNDLLSGNI+ +  Q     IDFEY +YN+RG ++GNHF+EYAG+  DYSLYPNK+ Q HF   Y R   K E   D++ K ++
Sbjct:  199 LEQRLA-QLNSPIVFCHNDLLSGNIIYDPSQNCASFIDFEYANYNFRGLELGNHFNEYAGFGPDYSLYPNKESQIHFLTDYHRSLFKTEPTQDELEKLYI 297

HSP 2 Score: 32.7278 bits (73), Expect = 1.097e-26
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQ 731
            N F LASHL+W  WA++Q
Sbjct:  300 NQFSLASHLYWGFWAIVQ 317
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: EKI1_HUMAN (Ethanolamine kinase 1 OS=Homo sapiens GN=ETNK1 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.959e-23
Identity = 46/105 (43.81%), Postives = 68/105 (64.76%), Query Frame = 2 
Query:  380 QELAGHLNAPVVFSHNDLLSGNIMVNDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGY-DCDYSLYPNKDEQNHFFRHYLRPDKP-----EEVSDKILKFFMLR 676
            +E+  +L +PVV  HNDLL  NI+ N++Q  +  ID+EY  YNY  YDIGNHF+E+AG  D DYSLYP+++ Q+ + R YL   K       EV++K ++   ++
Sbjct:  294 KEILSNLGSPVVLCHNDLLCKNIIYNEKQGDVQFIDYEYSGYNYLAYDIGNHFNEFAGVSDVDYSLYPDRELQSQWLRAYLEAYKEFKGFGTEVTEKEVEILFIQ 398

HSP 2 Score: 36.5798 bits (83), Expect = 2.959e-23
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQQRCSH-RF*YLG 764
            N F LASH FW LWALIQ + S   F +LG
Sbjct:  400 NQFALASHFFWGLWALIQAKYSTIEFDFLG 429
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: EKI1_MOUSE (Ethanolamine kinase 1 (Fragment) OS=Mus musculus GN=Etnk1 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.105e-22
Identity = 44/85 (51.76%), Postives = 56/85 (65.88%), Query Frame = 2 
Query:  380 QELAGHLNAPVVFSHNDLLSGNIMVNDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGY-DCDYSLYPNKDEQNHFFRHYLRPDK 631
            +EL   L +PVV  HNDLL  NI+ N++Q  +  ID+EY  YNY  YDIGNHF+E+AG  D DYSLYP+++ Q    R YL   K
Sbjct:  254 KELLSSLGSPVVLCHNDLLCKNIIYNEKQGDVQFIDYEYSGYNYLAYDIGNHFNEFAGVSDVDYSLYPDRELQGQCVRSYLEAYK 338

HSP 2 Score: 36.5798 bits (83), Expect = 1.105e-22
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQQRCSH-RF*YLG 764
            N F LASH FW LWALIQ + S   F +LG
Sbjct:  360 NQFALASHFFWGLWALIQAKYSTIEFDFLG 389
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: CHKA_HUMAN (Choline kinase alpha OS=Homo sapiens GN=CHKA PE=1 SV=3)

HSP 1 Score: 82.4185 bits (202), Expect = 1.095e-18
Identity = 44/87 (50.57%), Postives = 52/87 (59.77%), Query Frame = 2 
Query:  404 APVVFSHNDLLSGNIMV-----NDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGYDCDYS----------LYPNKDEQNHFFRHYL 619
            +PVVF HND   GNI++     N E++KL LIDFEY SYNYRG+DIGNHF E+  YD  Y            YP K +Q HF   YL
Sbjct:  298 SPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWM-YDYSYEKYPFFRANIRKYPTKKQQLHFISSYL 383

HSP 2 Score: 31.9574 bits (71), Expect = 1.095e-18
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQQRCS 743
            N F LASH  W LW+++Q + S
Sbjct:  410 NRFALASHFLWGLWSIVQAKIS 431
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: CHKA_RAT (Choline kinase alpha OS=Rattus norvegicus GN=Chka PE=2 SV=2)

HSP 1 Score: 82.4185 bits (202), Expect = 1.095e-18
Identity = 48/105 (45.71%), Postives = 59/105 (56.19%), Query Frame = 2 
Query:  362 YALRMEQE----LAGHLNAPVVFSHNDLLSGNIMV-----NDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGYDCDY----------SLYPNKDEQNHFFRHYL 619
            Y L +E E    L  +  +PVVF HND   GNI++     N E++KL LIDFEY SYNYRG+DIGNHF E+  YD  Y            YP + +Q HF   YL
Sbjct:  276 YNLPLELENLRSLLQYTRSPVVFCHNDCQEGNILLLEGQENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWM-YDYTYEKYPFFRANIQKYPTRKQQLHFISSYL 379

HSP 2 Score: 31.9574 bits (71), Expect = 1.095e-18
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQQRCS 743
            N F LASH  W LW+++Q + S
Sbjct:  406 NRFALASHFLWGLWSIVQAKIS 427
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: CHKA_MOUSE (Choline kinase alpha OS=Mus musculus GN=Chka PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 1.424e-18
Identity = 48/105 (45.71%), Postives = 59/105 (56.19%), Query Frame = 2 
Query:  362 YALRMEQE----LAGHLNAPVVFSHNDLLSGNIMV-----NDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGYDCDY----------SLYPNKDEQNHFFRHYL 619
            Y L +E E    L  +  +PVVF HND   GNI++     N E+ KL LIDFEY SYNYRG+DIGNHF E+  YD  Y            YP++ +Q HF   YL
Sbjct:  276 YNLPLELENLRSLLQYTRSPVVFCHNDCQEGNILLLEGQENSERRKLMLIDFEYSSYNYRGFDIGNHFCEWM-YDYTYEKYPFFRANIQKYPSRKQQLHFISSYL 379

HSP 2 Score: 31.9574 bits (71), Expect = 1.424e-18
Identity = 11/22 (50.00%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQQRCS 743
            N F LASH  W LW+++Q + S
Sbjct:  406 NRFALASHFLWGLWSIVQAKIS 427
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: EAS_DROME (Ethanolamine kinase OS=Drosophila melanogaster GN=eas PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 1.846e-18
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 2 
Query:  398 LNAPVVFSHNDLLSGNIMVNDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGYD-CDYSLYPNKDEQNHFFRHYL 619
            L++P+VFSHNDLL GN++       +  ID+EY  YN++ +DIGNHF+E  G D  DYS YP ++ Q  + R YL
Sbjct:  367 LDSPIVFSHNDLLLGNVIYTQSLNTVNFIDYEYADYNFQAFDIGNHFAEMCGVDEVDYSRYPKREFQLQWLRVYL 441

HSP 2 Score: 33.113 bits (74), Expect = 1.846e-18
Identity = 14/30 (46.67%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQ-QRCSHRF*YLG 764
            N F LASH+FW +W+L+Q +  +  F Y+G
Sbjct:  463 NQFALASHIFWTVWSLLQAEHSTIDFDYVG 492
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: CHKB_MOUSE (Choline/ethanolamine kinase OS=Mus musculus GN=Chkb PE=1 SV=3)

HSP 1 Score: 85.1149 bits (209), Expect = 1.857e-18
Identity = 47/111 (42.34%), Postives = 65/111 (58.56%), Query Frame = 2 
Query:  362 YALRME----QELAGHLNAPVVFSHNDLLSGNIMV---NDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGYDCDY----------SLYPNKDEQNHFFRHYLRPDKPEEV 643
            Y+L+ E    ++L     +PVVF HND+  GNI++    D  + L L+DFEY SYNYRG+DIGNHF E+  YD  Y          + YP +++Q HF RHYL   +  E+
Sbjct:  216 YSLKDEMNSLRKLLDDTPSPVVFCHNDIQEGNILLLSEPDSDDNLMLVDFEYSSYNYRGFDIGNHFCEWV-YDYTYEEWPFYKARPTDYPTREQQLHFIRHYLAEVQKGEI 325

HSP 2 Score: 28.4906 bits (62), Expect = 1.857e-18
Identity = 10/20 (50.00%), Postives = 13/20 (65.00%), Query Frame = 3 
Query:  684 FMLASHLFWALWALIQQRCS 743
            + LASH FW LW+ +Q   S
Sbjct:  345 YSLASHFFWGLWSTLQASMS 364
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: EKI_NEMVE (Probable ethanolamine kinase OS=Nematostella vectensis GN=etnk PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.862e-18
Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 2 
Query:  398 LNAPVVFSHNDLLSGNIMVNDEQEKLYLIDFEYGSYNYRGYDIGNHFSEYAGYD-CDYSLYPNKDEQNHFFRHYLR 622
            L + +VF+HNDLL  NI+ N +++ +  IDFEY + N   YDI NHF EYAG D  DYSLYP KD Q  F   YL+
Sbjct:  197 LESAIVFAHNDLLCKNIIYNKDKDSVCTIDFEYANPNPIAYDIANHFCEYAGVDEVDYSLYPQKDHQVKFLESYLK 272

HSP 2 Score: 31.187 bits (69), Expect = 1.862e-18
Identity = 11/22 (50.00%), Postives = 14/22 (63.64%), Query Frame = 3 
Query:  678 NTFMLASHLFWALWALIQQRCS 743
            N F LA+H FW +W L+Q   S
Sbjct:  298 NQFALAAHFFWGVWGLVQAHYS 319
[Close]
BLAST of EY832023 vs. ExPASy Swiss-Prot
Match: CHKB_HUMAN (Choline/ethanolamine kinase OS=Homo sapiens GN=CHKB PE=1 SV=3)

HSP 1 Score: 83.1889 bits (204), Expect = 3.140e-18
Identity = 44/92 (47.83%), Postives = 56/92 (60.87%), Query Frame = 2 
Query:  404 APVVFSHNDLLSGNIMVNDEQEK---LYLIDFEYGSYNYRGYDIGNHFSEYAGYDCDY----------SLYPNKDEQNHFFRHYLRPDKPEE 640
            +PVVF HND+  GNI++  E E    L L+DFEY SYNYRG+DIGNHF E+  YD  +          + YP +++Q HF RHYL   K  E
Sbjct:  234 SPVVFCHNDIQEGNILLLSEPENADSLMLVDFEYSSYNYRGFDIGNHFCEWV-YDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGE 324

HSP 2 Score: 29.6462 bits (65), Expect = 3.140e-18
Identity = 10/20 (50.00%), Postives = 14/20 (70.00%), Query Frame = 3 
Query:  684 FMLASHLFWALWALIQQRCS 743
            + LASH FW LW+++Q   S
Sbjct:  345 YALASHFFWGLWSILQASMS 364
The following BLAST results are available for this feature:
BLAST of EY832023 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13
Match NameE-valueIdentityDescription
EKIA_DICDI1.097e-2653.00Probable ethanolamine kinase A OS=Dictyostelium di... [more]
EKI1_HUMAN2.959e-2343.81Ethanolamine kinase 1 OS=Homo sapiens GN=ETNK1 PE=... [more]
EKI1_MOUSE1.105e-2251.76Ethanolamine kinase 1 (Fragment) OS=Mus musculus G... [more]
CHKA_HUMAN1.095e-1850.57Choline kinase alpha OS=Homo sapiens GN=CHKA PE=1 ... [more]
CHKA_RAT1.095e-1845.71Choline kinase alpha OS=Rattus norvegicus GN=Chka ... [more]
CHKA_MOUSE1.424e-1845.71Choline kinase alpha OS=Mus musculus GN=Chka PE=1 ... [more]
EAS_DROME1.846e-1848.00Ethanolamine kinase OS=Drosophila melanogaster GN=... [more]
CHKB_MOUSE1.857e-1842.34Choline/ethanolamine kinase OS=Mus musculus GN=Chk... [more]
EKI_NEMVE1.862e-1852.63Probable ethanolamine kinase OS=Nematostella vecte... [more]
CHKB_HUMAN3.140e-1847.83Choline/ethanolamine kinase OS=Homo sapiens GN=CHK... [more]

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Properties
Property NameValue
Genbank descriptionPT11-C2-300-090-E07-CT.F Poncirus trifoliata bark, greenhouse plant Citrus trifoliata cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY832023 ID=EY832023; Name=EY832023; organism=Citrus trifoliata; type=EST; length=854bp
CCACGCGTCCGCCCACGCGTCCGAAATCATACATGCATTTAATCATGTAT
TTTTTTTTTTATCCCTGACCAAGTTGGATGGTCTGGTCCACCTGAAGATA
CATACTTGACTCCCCTCCAAATGATAATCTCAATCTGGAAAATTCTACTT
CAGCATTAGGAAGCACATGTCACTAGCCAAGAATAATAGTCTAAACCCCT
ATACAATTGGTTGCCTTCTTTCTACCAAATTATTGTTTAGGGTGGGAGGT
AGCCGTGAAAGGATTGTGGAAGAGTAATTTGTGCAAATGGATGATACAAT
ATGTTTATTATGCTGATTTTACTCATTCATAAAATAAAGAACTTTTATCC
GATTTATAAGATATGCCCTGAGAATGGAACAGGAATTGGCTGGCCATCTT
AATGCTCCGGTGGTGTTTTCTCATAATGACTTGCTTTCTGGGAATATAAT
GGTTAATGATGAACAAGAGAAACTCTACTTGATTGATTTTGAGTACGGGT
CATACAATTACAGAGGCTATGACATTGGAAATCACTTCAGTGAATATGCA
GGCTATGATTGTGACTATAGTTTATACCCAAATAAAGATGAGCAAAATCA
TTTCTTCAGGCATTATTTACGACCTGACAAACCGGAAGAGGTCTCTGACA
AGATCTTGAAGTTCTTTATGTTGAGGCAATACATTCATGTTGGCCTCACA
TTTATTTTGGGCTTTGTGGGCCTTAATCCAGCAAAGATGCTCCCATAGAT
TTTGATATCTTGGTATTCCTCTACGAACATGATACAAAACCGAAGAATGG
CGCTCATCCATGAGGCGTACCCATCGTTGTAGACTCTTATATCCACAACT
TGAG
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