FC869023

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ER25A_DICDI (Putative C-4 methylsterol oxidase DDB_G0270946 OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2)

HSP 1 Score: 185.652 bits (470), Expect = 2.739e-50
Identity = 79/169 (46.75%), Postives = 110/169 (65.09%), Query Frame = 2

Query:  200 KNNSPAAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHHRL 706
            K N   +Q  C  +++L    + LP+M    P  K +G+ +  PLPS   +L  ++  FI+EDF FYW HR LH    YK++H VHH+YA+PFG+T+EYAHP E + LG  T++GP +    L TLW+W+ +R+ +TVE H GY FPWS +N IP +GGA FHDYHH +
Sbjct:   63 KENDWKSQTYCAIKVILTQVLIQLPMMYIFDPAIKAIGLSARAPLPSIPYLLLTLVSSFIIEDFYFYWAHRALHHGIWYKYIHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSRDLFTLWVWLGVRLYQTVECHSGYDFPWSFTNLIPFWGGAPFHDYHHEV 231          

HSP 2 Score: 34.2686 bits (77), Expect = 2.739e-50
Identity = 16/55 (29.09%), Postives = 27/55 (49.09%), Query Frame = 1

Query:   40 IESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            IE  W   + ++ +  L   G+F  H+  +F   +PF+  +   +  KYKIQ  K
Sbjct:    9 IEPYWFKFVDYYGEEFLLTYGTFIAHQVFYFGCFIPFLIADFIPFFRKYKIQQTK 63          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ER25B_DICDI (Putative C-4 methylsterol oxidase DDB_G0269788 OS=Dictyostelium discoideum GN=DDB_G0269788 PE=3 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 1.030e-49
Identity = 77/167 (46.11%), Postives = 107/167 (64.07%), Query Frame = 2

Query:  200 KNNSPAAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            K N    Q  CI ++L+ H  V LP+M    P  K +G+ +  PLPS   ++  I   F++EDF FYW HR LH  + YKH+H VHH++A PFG+T+EYAHP E + LG  T++GP +    L TLW+W+  R+ +TVE H GY FPW+ +  IP +GG+ FHD+HH
Sbjct:   63 KKNEWKTQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIEDFYFYWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRDLFTLWVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHH 229          

HSP 2 Score: 36.965 bits (84), Expect = 1.030e-49
Identity = 18/55 (32.73%), Postives = 28/55 (50.91%), Query Frame = 1

Query:   40 IESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            IE  W   + ++ +  L   G+F  HE  +F + +PF   +   +L KYKIQ  K
Sbjct:    9 IEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTK 63          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_SCHPO (C-4 methylsterol oxidase OS=Schizosaccharomyces pombe GN=erg25 PE=2 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 5.239e-42
Identity = 71/167 (42.51%), Postives = 104/167 (62.28%), Query Frame = 2

Query:  215 AAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAI-----TGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            A Q +C   +LL HF V LP +    P+    G+ +S+P P    ++ QI  +F LED   YW HR+ H    Y+ +H VHH Y+ PFGL++EYAHP EI+ LG  T+  P +        HL+T+++W+ LR+ + V++H GY FPWSL+ F+P++ GA+ HDYHH
Sbjct:   90 AEQWECTRLVLLSHFTVELPQIWLFDPMCATFGLSTSVPFPPVTKMIWQITLFFFLEDTWHYWAHRLFHYGIFYRFIHKVHHRYSAPFGLSAEYAHPLEIILLGAGTVFVPLMWCYFTHDLHLVTMYIWITLRLFQAVDSHAGYDFPWSLNKFLPIWAGADHHDYHH 256          

HSP 2 Score: 31.5722 bits (70), Expect = 5.239e-42
Identity = 18/58 (31.03%), Postives = 31/58 (53.45%), Query Frame = 1

Query:   34 SIIESGWRYLITHFSDFQLAC-LGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            + +E  W      F +  +A  L SF LHE I+F   +P++ ++   +  ++KIQ KK
Sbjct:   28 NFVEQLWLAYYKWFDNDVVATGLMSFLLHELIYFGRCIPWMIIDAMPYFRRWKIQPKK 85          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_CHICK (C-4 methylsterol oxidase OS=Gallus gallus GN=SC4MOL PE=2 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 5.240e-42
Identity = 63/161 (39.13%), Postives = 98/161 (60.87%), Query Frame = 2

Query:  221 QEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLP-LPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            Q KC   L+  HF + LP++  +Y   +Y  +      +P W V+L+Q     ++ED   Y+ HR+LH K +YK++H VHHE+ +PFG+ +EYAHP E L LG    +G  +   H++ LW W++ R++ET++ H GY  P +  + +P Y GA FHD+HH
Sbjct:   94 QWKCFKTLIFNHFFIQLPLICGTYYFTEYFNIPYEWEEMPRWYVLLAQCFGCAVIEDAWHYFLHRLLHHKRIYKYIHKVHHEFVSPFGMQAEYAHPLETLILGAGFFIGIVVFCNHVVLLWAWVICRLMETIDVHSGYDIPLNPLHLVPFYAGARFHDFHH 254          

HSP 2 Score: 45.4394 bits (106), Expect = 5.240e-42
Identity = 18/54 (33.33%), Postives = 33/54 (61.11%), Query Frame = 1

Query:   43 ESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            ++ W Y++ +++ FQ+A  GS  +HE+ +FL  +P    +   ++ KYKIQ  K
Sbjct:   34 KNAWNYMLDNYTKFQIATWGSLLVHEASYFLLCVPGFIFQFIPYMQKYKIQPDK 87          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_MOUSE (C-4 methylsterol oxidase OS=Mus musculus GN=Sc4mol PE=2 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 5.240e-42
Identity = 62/170 (36.47%), Postives = 101/170 (59.41%), Query Frame = 2

Query:  194 RQKNNSPAAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLP-LPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            + K  +   Q KC+ ++L  HF + LP++  +Y   ++  +      +P W + L++ +   ++ED   Y+ HR+LH K +YK++H VHHE+  PFG+ +EYAHP E L LG    +G  +   H++ LW W+ +R+LET++ H GY  P +  N +P Y GA  HD+HH
Sbjct:   85 KDKPETFEGQWKCLKKILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYLTLARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLVPFYTGARHHDFHH 254          

HSP 2 Score: 48.1358 bits (113), Expect = 5.240e-42
Identity = 20/54 (37.04%), Postives = 34/54 (62.96%), Query Frame = 1

Query:   43 ESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            ++ W Y++ +++ FQ+A  GS  +HE+I+FL  LP    +   ++ KYKIQ  K
Sbjct:   34 KNAWVYMLDNYTKFQIATWGSLIVHEAIYFLFSLPGFLFQFIPYMRKYKIQKDK 87          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_RAT (C-4 methylsterol oxidase OS=Rattus norvegicus GN=Sc4mol PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.159e-41
Identity = 63/170 (37.06%), Postives = 100/170 (58.82%), Query Frame = 2

Query:  194 RQKNNSPAAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLP-LPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            + K  +   Q KC+  +L  HF + LP++  +Y   ++  +      +P W   L++ +   ++ED   Y+ HR+LH K +YK++H VHHE+  PFG+ +EYAHP E L LG    +G  +   H++ LW W+ +R+LET++ H GY  P +  N+IP Y GA  HD+HH
Sbjct:   85 KDKPETFEGQWKCLKGILFNHFFIQLPLICGTYYFTEFFNIPYDWERMPRWYFTLARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTMRLLETIDVHSGYDIPLNPLNYIPFYTGARHHDFHH 254          

HSP 2 Score: 47.7506 bits (112), Expect = 1.159e-41
Identity = 20/54 (37.04%), Postives = 34/54 (62.96%), Query Frame = 1

Query:   43 ESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            ++ W Y++ +++ FQ+A  GS  +HE+I+FL  LP    +   ++ KYKIQ  K
Sbjct:   34 KNAWVYMLDNYTKFQIATWGSLIVHETIYFLFSLPGFLFQFIPFMRKYKIQKDK 87          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_PONAB (C-4 methylsterol oxidase OS=Pongo abelii GN=SC4MOL PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.159e-41
Identity = 65/175 (37.14%), Postives = 101/175 (57.71%), Query Frame = 2

Query:  188 KFRQKNNSPAAQE---KCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLP-LPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            K++ + + P   E   KC   LL  HF + LP++  +Y   +Y  +      +P W  +L++     ++ED   Y+ HR+LH K +YK++H VHHE+  PFG+ +EYAHP E L LG    +G  +   H++ LW W+ +R+LET++ H GY  P +  N IP Y G+  HD+HH
Sbjct:   80 KYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHH 254          

HSP 2 Score: 48.1358 bits (113), Expect = 1.159e-41
Identity = 19/54 (35.19%), Postives = 34/54 (62.96%), Query Frame = 1

Query:   43 ESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            ++ W Y++ +++ FQ+A  GS  +HE+++FL  LP    +   ++ KYKIQ  K
Sbjct:   34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDK 87          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_HUMAN (C-4 methylsterol oxidase OS=Homo sapiens GN=SC4MOL PE=1 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 1.159e-41
Identity = 65/175 (37.14%), Postives = 101/175 (57.71%), Query Frame = 2

Query:  188 KFRQKNNSPAAQE---KCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLP-LPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            K++ + + P   E   KC   LL  HF + LP++  +Y   +Y  +      +P W  +L++     ++ED   Y+ HR+LH K +YK++H VHHE+  PFG+ +EYAHP E L LG    +G  +   H++ LW W+ +R+LET++ H GY  P +  N IP Y G+  HD+HH
Sbjct:   80 KYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHH 254          

HSP 2 Score: 48.1358 bits (113), Expect = 1.159e-41
Identity = 19/54 (35.19%), Postives = 34/54 (62.96%), Query Frame = 1

Query:   43 ESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            ++ W Y++ +++ FQ+A  GS  +HE+++FL  LP    +   ++ KYKIQ  K
Sbjct:   34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDK 87          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_MACFA (C-4 methylsterol oxidase OS=Macaca fascicularis GN=SC4MOL PE=2 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 4.355e-41
Identity = 65/175 (37.14%), Postives = 101/175 (57.71%), Query Frame = 2

Query:  188 KFRQKNNSPAAQE---KCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLP-LPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            K++ + + P   E   KC   LL  HF + LP++  +Y   +Y  +      +P W  +L++     ++ED   Y+ HR+LH K +YK++H VHHE+  PFG+ +EYAHP E L LG    +G  +   H++ LW W+ +R+LET++ H GY  P +  N IP Y G+  HD+HH
Sbjct:   80 KYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFMHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHH 254          

HSP 2 Score: 45.8246 bits (107), Expect = 4.355e-41
Identity = 18/54 (33.33%), Postives = 33/54 (61.11%), Query Frame = 1

Query:   43 ESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            ++ W Y++ +++ FQ+A  GS  +HE+++F   LP    +   ++ KYKIQ  K
Sbjct:   34 KNAWNYMLNNYTKFQIATWGSLIVHEALYFSFCLPGFLFQFIPYMKKYKIQKDK 87          

BLAST of FC869023 vs. ExPASy Swiss-Prot
Match: ERG25_YEAST (C-4 methylsterol oxidase OS=Saccharomyces cerevisiae GN=ERG25 PE=1 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 9.626e-41
Identity = 78/176 (44.32%), Postives = 111/176 (63.07%), Query Frame = 2

Query:  197 QKNNSPAAQEK--CITRLLLYHFGVN-LPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILEDFVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAI----TGP-HLMTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGAEFHDYHH 700
            Q    P+A+E+  C+  +LL HF V  +P+  F +P+ + +G+   +P PS K +  +I  +F+LED   YW HR+ H    YK++H  HH YA PFGL++EYAHPAE L LGF T+  P +    TG  HL TL +W+ LR+ + V++H GY FPWSL+  +P + GAE HD HH
Sbjct:   88 QPTKIPSAKEQLYCLKSVLLSHFLVEAIPIWTF-HPMCEKLGITVEVPFPSLKTMALEIGLFFVLEDTWHYWAHRLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMYTGKLHLFTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHHDLHH 262          

HSP 2 Score: 28.8758 bits (63), Expect = 9.626e-41
Identity = 15/58 (25.86%), Postives = 31/58 (53.45%), Query Frame = 1

Query:   34 SIIESGWRYLITHFSDFQLAC-LGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKK 204
            + +E  W    ++ ++  LA  L  F LHE ++F   LP+  +++  +  ++K+Q  K
Sbjct:   34 NFMEKYWAAWYSYMNNDVLATGLMFFLLHEFMYFFRCLPWFIIDQIPYFRRWKLQPTK 91