Effects of antioxidants on Agrobacterium-mediated transformation and accelerated production of transgenic plants of Mexican lime (Citrus aurantifolia Swingle)

Publication Overview
TitleEffects of antioxidants on Agrobacterium-mediated transformation and accelerated production of transgenic plants of Mexican lime (Citrus aurantifolia Swingle)
AuthorsDutt M, Vasconcellos M, Grosser JW
TypeJournal Article
Journal NamePlant cell, tissue, and organ culture
Volume107
Issue1
Year2011
Page(s)79-89
CitationDutt M, Vasconcellos M, Grosser JW. Effects of antioxidants on Agrobacterium-mediated transformation and accelerated production of transgenic plants of Mexican lime (Citrus aurantifolia Swingle). Plant cell, tissue, and organ culture. 2011; 107(1):79-89.

Abstract

Four antioxidants including glycine betaine, glutathione, lipoic acid, and polyvinylpyrrolidone were evaluated to improve transformation efficiency of Mexican lime, a precocious but recalcitrant citrus cultivar to Agrobacterium mediated transformation. Lipoic acid substantially improved the transformation efficiency of Mexican lime by aiding in callus development and improving shoot growth from cut ends of epicotyl segments co-cultivated with Agrobacterium. Glycine betaine was moderately beneficial while glutathione and polyvinylpyrrolidone did not have the ability to improve the transformation efficiency. A bi-functional gus-egfp fusion gene used in the study enabled visual identification of transformants and quantitative analysis of gene expression. We describe an improved protocol that allows regenerated transgenic plants to flower within 20–22 months after in vitro regeneration. This enabled the rapid evaluation of transgenic flowers and fruits. Gene expression levels could not be correlated to copy number as determined using Southern blot analysis. Our improved transformation method facilitates the rapid production and evaluation of transgenic plants, especially regarding the functional analysis of transgenes in citrus.
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
Mexican limeMexican limebreeding_research_material
Features
This publication contains information about 7 features:
Feature NameUniquenameType
GUS-F51GUS-F51genetic_marker
GUS-F32GUS-F32genetic_marker
GUS-FRTGUS-FRTgenetic_marker
GUS-RRTGUS-RRTgenetic_marker
EG-FRTEG-FRTgenetic_marker
EG-RRTEG-RRTgenetic_marker
EG-32EG-32genetic_marker
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2011
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s11240-011-9959-x
KeywordsAgrobacterium, Citrus aurantiifolia, Southern blotting, antioxidant activity, antioxidants, betaine, crop production, cultivars, flowers, fruits, gene expression, gene fusion, glutathione, in vitro regeneration, limes, lipoic acid, quantitative analysis, rapid methods, shoots, transgenic plants
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4675543