FC920652 | Citrus Genome Database

Overview


Name	FC920652
Unique Name	FC920652
Type	EST
Organism	Citrus clementina (Clementine)
Sequence length	712

Libraries


Library Name	Type
RVDevelop1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
FC920652_ssr29	microsatellite	FC920652_ssr29:29..38.	BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig10339	contig	Ccv1_Contig10339:7..720.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO_RICCO (Enolase OS=Ricinus communis PE=2 SV=1)

HSP 1 Score: 339.732 bits (870), Expect = 1.285e-98
Identity = 166/186 (89.25%), Postives = 177/186 (95.16%), Query Frame = 2

Query:   77 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            MAITI +V+ARQIFDSRGNPTVE D+  SDGH+ARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAV NVN+IIGPAL GKDPTEQTA+DN+MVQ+LDGTVNEWGWCKQKLGANAILAVSLA+CKAGAHVK IPLYKHIA L+GNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS FK+
Sbjct:    1 MAITIVSVRARQIFDSRGNPTVEADIKLSDGHLARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVENVNSIIGPALIGKDPTEQTALDNFMVQELDGTVNEWGWCKQKLGANAILAVSLALCKAGAHVKGIPLYKHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE 186

HSP 2 Score: 41.9726 bits (97), Expect = 1.285e-98
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMG EVYHHLK+VIKK
Sbjct:  184 FKEAMKMGAEVYHHLKSVIKK 204

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO1_HEVBR (Enolase 1 OS=Hevea brasiliensis GN=ENO1 PE=1 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 9.053e-97
Identity = 164/186 (88.17%), Postives = 174/186 (93.55%), Query Frame = 2

Query:   77 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            MAITI +V+ARQIFDSRGNPTVE DV  SDG++ARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAV NVN IIGPAL GKDPT+Q  IDN+MVQQLDGTVNEWGWCKQKLGANAILAVSLA+CKAGAHVK IPLY+HIA L+GNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS FK+
Sbjct:    1 MAITIVSVRARQIFDSRGNPTVEADVKLSDGYLARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVENVNIIIGPALVGKDPTDQVGIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKGIPLYEHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE 186

HSP 2 Score: 41.9726 bits (97), Expect = 9.053e-97
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMG EVYHHLK+VIKK
Sbjct:  184 FKEAMKMGAEVYHHLKSVIKK 204

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO2_HEVBR (Enolase 2 OS=Hevea brasiliensis GN=ENO2 PE=1 SV=1)

HSP 1 Score: 332.798 bits (852), Expect = 1.541e-96
Identity = 163/186 (87.63%), Postives = 173/186 (93.01%), Query Frame = 2

Query:   77 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            MAITI +V+ARQIFDSRGNPTVE DV  SDG++ARAAVP GASTGIYEALELRDGGSDYLGKGVSKAV NVN IIGPAL GKDPT+Q  IDN+MVQQLDGTVNEWGWCKQKLGANAILAVSLA+CKAGAHVK IPLYKH+A L+GNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS FK+
Sbjct:    1 MAITIVSVRARQIFDSRGNPTVEADVKLSDGYLARAAVPRGASTGIYEALELRDGGSDYLGKGVSKAVENVNIIIGPALVGKDPTDQVGIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAHVKGIPLYKHVANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE 186

HSP 2 Score: 41.9726 bits (97), Expect = 1.541e-96
Identity = 18/21 (85.71%), Postives = 20/21 (95.24%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMG EVYHHLK+VIKK
Sbjct:  184 FKEAMKMGAEVYHHLKSVIKK 204

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO1_MAIZE (Enolase 1 OS=Zea mays GN=ENO1 PE=2 SV=1)

HSP 1 Score: 323.939 bits (829), Expect = 2.020e-93
Identity = 162/186 (87.10%), Postives = 169/186 (90.86%), Query Frame = 2

Query:   77 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            MA+TIT VKARQIFDSRGNPTVEVDV  SDG  AR AVPSGASTGIYEALELRDGGSDYLGKGV KAVSNVN IIGPA+ GKDPTEQ  IDN+MVQQLDGT NEWGWCKQKLGANAILAVSLA+CKAGA VKKIPLY+HIA L+GNK LVLPVPAFNVINGGSHAGNKLAMQEFMILP GAS FK+
Sbjct:    1 MAVTITWVKARQIFDSRGNPTVEVDVGLSDGSYARGAVPSGASTGIYEALELRDGGSDYLGKGVLKAVSNVNNIIGPAIVGKDPTEQVEIDNFMVQQLDGTSNEWGWCKQKLGANAILAVSLAVCKAGAMVKKIPLYQHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGASSFKE 186

HSP 2 Score: 40.4318 bits (93), Expect = 2.020e-93
Identity = 17/21 (80.95%), Postives = 21/21 (100.00%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMGVEVYH+LK++IKK
Sbjct:  184 FKEAMKMGVEVYHNLKSIIKK 204

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO2_MAIZE (Enolase 2 OS=Zea mays GN=ENO2 PE=2 SV=1)

HSP 1 Score: 318.546 bits (815), Expect = 9.958e-93
Identity = 157/186 (84.41%), Postives = 170/186 (91.40%), Query Frame = 2

Query:   77 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            MA TI +VKARQIFDSRGNPTVEVDV  SDG  ARAAVPSGASTG+YEALELRDGGS YLGKGVSKAV+NVN++IGPAL GKDPT QT IDN+MVQQLDGT NEWGWCKQKLGANAILAVSLA+CKAGA +K+IPLY+HIA L+GNK LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+ FK+
Sbjct:    1 MAATIQSVKARQIFDSRGNPTVEVDVFCSDGTFARAAVPSGASTGVYEALELRDGGSYYLGKGVSKAVNNVNSVIGPALIGKDPTAQTEIDNFMVQQLDGTKNEWGWCKQKLGANAILAVSLAVCKAGASIKRIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAASFKE 186

HSP 2 Score: 43.5134 bits (101), Expect = 9.958e-93
Identity = 19/21 (90.48%), Postives = 21/21 (100.00%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMGVEVYHHLK+VIKK
Sbjct:  184 FKEAMKMGVEVYHHLKSVIKK 204

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO_MESCR (Enolase OS=Mesembryanthemum crystallinum GN=PGH1 PE=2 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 4.908e-92
Identity = 155/184 (84.24%), Postives = 168/184 (91.30%), Query Frame = 2

Query:   83 ITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            +TI  VKARQI+DSRGNPTVE D+   DG  ARAAVPSGASTG+YEALELRDGG DY+GKGV KAV NVN IIGPAL GKDPT+QTAIDN+MVQQLDGTVNEWGWCKQKLGANAILAVSLA+CKAGA VKKIPLY+HIAE++GNKN+VLPVPAFNVINGGSHAGNKLAMQEFMILP GAS FK+
Sbjct:    2 VTIKCVKARQIYDSRGNPTVEADIHLDDGTYARAAVPSGASTGVYEALELRDGGKDYMGKGVYKAVKNVNEIIGPALVGKDPTQQTAIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAQVKKIPLYQHIAEIAGNKNMVLPVPAFNVINGGSHAGNKLAMQEFMILPTGASSFKE 185

HSP 2 Score: 38.5058 bits (88), Expect = 4.908e-92
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMG EVYH+LK+VIKK
Sbjct:  183 FKEAMKMGSEVYHNLKSVIKK 203

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO_ORYSJ (Enolase OS=Oryza sativa subsp. japonica GN=ENO1 PE=1 SV=2)

HSP 1 Score: 318.546 bits (815), Expect = 6.403e-92
Identity = 157/186 (84.41%), Postives = 168/186 (90.32%), Query Frame = 2

Query:   77 MAITITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            MA TI +VKARQIFDSRGNPTVEVDV  SDG  ARAAVPSGASTG+YEALELRDGGSDYLGKGVSKAV NVN++I PAL GKDPT Q  +DN+MVQQLDGT NEWGWCKQKLGANAILAVSLAICKAGA +KKIPLY+HIA L+GNK LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+ FK+
Sbjct:    1 MAATIVSVKARQIFDSRGNPTVEVDVCCSDGTFARAAVPSGASTGVYEALELRDGGSDYLGKGVSKAVDNVNSVIAPALIGKDPTSQAELDNFMVQQLDGTKNEWGWCKQKLGANAILAVSLAICKAGAIIKKIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAASFKE 186

HSP 2 Score: 40.817 bits (94), Expect = 6.403e-92
Identity = 18/21 (85.71%), Postives = 21/21 (100.00%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMGVEVYH+LK+VIKK
Sbjct:  184 FKEAMKMGVEVYHNLKSVIKK 204

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO_SOLLC (Enolase OS=Solanum lycopersicum GN=PGH1 PE=2 SV=1)

HSP 1 Score: 315.849 bits (808), Expect = 1.090e-91
Identity = 154/183 (84.15%), Postives = 170/183 (92.90%), Query Frame = 2

Query:   86 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            TI ++KARQIFDSRGNPTVEVDV  S+G  ARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAV+NVN+IIGPAL GKDPT+QT +DN+MV QLDGT NEWGWCK+KLGANAILAVSLA+CKAGA V+ +PLYKHIA+L+GNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA+ FK+
Sbjct:    3 TIKSIKARQIFDSRGNPTVEVDVHISNGVFARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVNNVNSIIGPALVGKDPTDQTGLDNFMVHQLDGTQNEWGWCKEKLGANAILAVSLAVCKAGAAVRNVPLYKHIADLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFKE 185

HSP 2 Score: 42.743 bits (99), Expect = 1.090e-91
Identity = 19/21 (90.48%), Postives = 20/21 (95.24%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMG EVYHHLKAVIKK
Sbjct:  183 FKEAMKMGCEVYHHLKAVIKK 203

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO_ALNGL (Enolase OS=Alnus glutinosa GN=PGH1 PE=2 SV=1)

HSP 1 Score: 312.383 bits (799), Expect = 3.156e-91
Identity = 157/183 (85.79%), Postives = 168/183 (91.80%), Query Frame = 2

Query:   89 ITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAID-NYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            IT +KARQIFDSRGNPTVE +VTT++G V+RAAVPSGASTG+YEALELRDGGSDYLGKGV KAV NVN IIGPAL GKD TEQTAID ++M QQLDGTVNEWGWCKQKLGANAILAVSLA+CKAGA VKKIPLYKHIA L+GN  LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS FK+
Sbjct:    4 ITHIKARQIFDSRGNPTVEAEVTTANGVVSRAAVPSGASTGVYEALELRDGGSDYLGKGVLKAVDNVNKIIGPALIGKDATEQTAIDIDFMFQQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGASVKKIPLYKHIANLAGNPKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE 186

HSP 2 Score: 44.669 bits (104), Expect = 3.156e-91
Identity = 20/21 (95.24%), Postives = 21/21 (100.00%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMGVEVYHHLKAVIKK
Sbjct:  184 FKEAMKMGVEVYHHLKAVIKK 204

[Close]

BLAST of FC920652 vs. ExPASy Swiss-Prot
Match: ENO_ARATH (Enolase OS=Arabidopsis thaliana GN=ENO PE=1 SV=1)

HSP 1 Score: 311.612 bits (797), Expect = 1.192e-90
Identity = 155/183 (84.70%), Postives = 165/183 (90.16%), Query Frame = 2

Query:   86 TITAVKARQIFDSRGNPTVEVDVTTSDGHVARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVSNVNAIIGPALAGKDPTEQTAIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLAICKAGAHVKKIPLYKHIAELSGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASCFKK 634
            TIT VKARQIFDSRGNPTVEVD+ TS+G    AAVPSGASTGIYEALELRDGGSDYLGKGVSKAV NVN IIGPAL GKDPT+QTAIDN+MV +LDGT NEWGWCKQKLGANAILAVSLA+CKAGA V  IPLYKHIA L+GN  +VLPVPAFNVINGGSHAGNKLAMQEFMILPVGA+ FK+
Sbjct:    3 TITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKE 185

HSP 2 Score: 43.5134 bits (101), Expect = 1.192e-90
Identity = 19/21 (90.48%), Postives = 21/21 (100.00%), Query Frame = 1

Query:  625 FQEAMKMGVEVYHHLKAVIKK 687
            F+EAMKMGVEVYHHLK+VIKK
Sbjct:  183 FKEAMKMGVEVYHHLKSVIKK 203

The following BLAST results are available for this feature:

BLAST of FC920652 vs. ExPASy Swiss-Prot

BLAST of FC920652 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
ENO_RICCO	1.285e-98	89.25	Enolase OS=Ricinus communis PE=2 SV=1	[more]
ENO1_HEVBR	9.053e-97	88.17	Enolase 1 OS=Hevea brasiliensis GN=ENO1 PE=1 SV=1	[more]
ENO2_HEVBR	1.541e-96	87.63	Enolase 2 OS=Hevea brasiliensis GN=ENO2 PE=1 SV=1	[more]
ENO1_MAIZE	2.020e-93	87.10	Enolase 1 OS=Zea mays GN=ENO1 PE=2 SV=1	[more]
ENO2_MAIZE	9.958e-93	84.41	Enolase 2 OS=Zea mays GN=ENO2 PE=2 SV=1	[more]
ENO_MESCR	4.908e-92	84.24	Enolase OS=Mesembryanthemum crystallinum GN=PGH1 P...	[more]
ENO_ORYSJ	6.403e-92	84.41	Enolase OS=Oryza sativa subsp. japonica GN=ENO1 PE...	[more]
ENO_SOLLC	1.090e-91	84.15	Enolase OS=Solanum lycopersicum GN=PGH1 PE=2 SV=1	[more]
ENO_ALNGL	3.156e-91	85.79	Enolase OS=Alnus glutinosa GN=PGH1 PE=2 SV=1	[more]
ENO_ARATH	1.192e-90	84.70	Enolase OS=Arabidopsis thaliana GN=ENO PE=1 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	C32011C09EF RVDevelop1 Citrus clementina cDNA clone C32011C09, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FC920652 ID=FC920652; Name=FC920652; organism=Citrus clementina; type=EST; length=712bp
GAGAACAAGACTCTTCTCGTTCCACAGACTCTCTCTCTCGTTCTAGATCT
TTCTTCGGTTTTCCAGCTTTCAGAAAATGGCCATAACCATCACTGCCGTC
AAAGCCAGGCAGATCTTTGACAGCCGTGGAAATCCAACGGTGGAGGTCGA
CGTGACAACATCAGATGGCCACGTGGCTAGAGCTGCTGTTCCAAGCGGTG
CATCCACCGGAATTTATGAGGCGCTTGAACTAAGAGACGGAGGCTCTGAT
TACCTAGGCAAGGGTGTATCGAAGGCTGTTAGCAATGTCAACGCCATCAT
TGGCCCTGCACTCGCTGGCAAGGACCCAACTGAGCAGACTGCTATTGACA
ACTATATGGTACAACAACTTGATGGAACTGTAAATGAGTGGGGTTGGTGC
AAACAGAAGCTTGGAGCAAACGCCATTTTGGCTGTCTCCCTAGCTATCTG
CAAGGCTGGTGCACATGTCAAGAAGATTCCTCTTTACAAGCATATTGCCG
AACTCTCTGGTAACAAGAATTTGGTGTTGCCAGTGCCAGCATTCAATGTC
ATTAATGGTGGATCACATGCTGGAAACAAACTTGCTATGCAGGAGTTTAT
GATTCTTCCTGTAGGAGCTTCCTGTTTCAAGAAGCCATGAAGATGGGTGT
AGAAGTATATCACCATCTGAAGGCTGTGATTAAAAAGAATACGGCCAGGA
TGCTACAATGTT