FC925768

Overview
NameFC925768
Unique NameFC925768
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length700
Libraries
Library NameType
TFcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig14677 contig Ccv1_Contig14677:1..699. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP12_ORYSJ (Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica GN=PIP1-2 PE=2 SV=3)

HSP 1 Score: 242.662 bits (618), Expect = 2.160e-91
Identity = 119/136 (87.50%), Postives = 125/136 (91.91%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQTQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVRLGANKF+ERQPIGTAAQ  D KDY EPPPAPLFEPGELKSWSFYRAGIAEF+ATFLFLYI+VLTVMGV  S +KC+TV IQGIAW+FGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVRLGANKFSERQPIGTAAQGSDDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFMATFLFLYITVLTVMGVNNSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALF 136

HSP 2 Score: 114.775 bits (286), Expect = 2.160e-91
Identity = 58/72 (80.56%), Postives = 62/72 (86.11%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            LFYMVMQCLGAICGAGVVKGF   G Y    GGA+VVA GYTKGDGLGAEI+GTF+LVY VFSATDAKR+AR
Sbjct:  135 LFYMVMQCLGAICGAGVVKGFQ-KGLYETTGGGANVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRNAR 205
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP12_MAIZE (Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 8.927e-90
Identity = 119/137 (86.86%), Postives = 126/137 (91.97%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQ-TQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVRLGANKF+ERQPIGTAAQ   D KDY EPPPAPLFEPGELKSWSFYRAGIAEF+ATFLFLYI++LTVMGV KS +KC+TV IQGIAW+FGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVRLGANKFSERQPIGTAAQGAADDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFVATFLFLYITILTVMGVSKSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALF 137

HSP 2 Score: 110.923 bits (276), Expect = 8.927e-90
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            LFY++MQCLGA+CGAGVVKGF   G Y    GGA+VVA GYTKGDGLGAEI+GTF+LVY VFSATDAKR+AR
Sbjct:  136 LFYIIMQCLGAVCGAGVVKGFQ-QGLYMGNGGGANVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRNAR 206
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP13_MAIZE (Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2 SV=1)

HSP 1 Score: 239.965 bits (611), Expect = 2.585e-89
Identity = 120/140 (85.71%), Postives = 126/140 (90.00%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQT----QDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVRLGANKF+ERQPIGTAAQ      D KDY EPPPAPLFEPGELKSWSFYRAGIAEF+ATFLFLYI+VLTVMGV KS +KC+TV IQGIAW+FGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVRLGANKFSERQPIGTAAQGAGAGDDDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFVATFLFLYITVLTVMGVSKSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIF 140

HSP 2 Score: 110.538 bits (275), Expect = 2.585e-89
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            +FY++MQCLGAICGAGVVKGF   G Y    GGA+VVA GYTKGDGLGAEI+GTF+LVY VFSATDAKR+AR
Sbjct:  139 IFYIIMQCLGAICGAGVVKGFQ-QGLYMGNGGGANVVAPGYTKGDGLGAEIVGTFILVYTVFSATDAKRNAR 209
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP11_ORYSJ (Aquaporin PIP1-1 OS=Oryza sativa subsp. japonica GN=PIP1-1 PE=2 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 3.372e-89
Identity = 117/137 (85.40%), Postives = 127/137 (92.70%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQ-TQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVRLGAN+++ERQPIGTAAQ   D KDY EPPPAPLFEPGELKSWSFYRAGIAEF+ATFLFLYI++LTVMGV KS++KC+TV IQGIAW+FGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVRLGANRYSERQPIGTAAQGAGDDKDYKEPPPAPLFEPGELKSWSFYRAGIAEFVATFLFLYITILTVMGVSKSSSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIF 137

HSP 2 Score: 111.309 bits (277), Expect = 3.372e-89
Identity = 56/72 (77.78%), Postives = 62/72 (86.11%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            +FY+VMQCLGAICGAGVVKGF   G Y    GGA+VVA GYTKGDGLGAEI+GTF+LVY VFSATDAKR+AR
Sbjct:  136 IFYIVMQCLGAICGAGVVKGFQ-QGLYMGNGGGANVVASGYTKGDGLGAEIVGTFILVYTVFSATDAKRNAR 206
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP14_ARATH (Probable aquaporin PIP1-4 OS=Arabidopsis thaliana GN=PIP1.4 PE=1 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 3.689e-88
Identity = 115/136 (84.56%), Postives = 123/136 (90.44%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQTQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVR+GANKF ERQPIGT+AQ+ D KDY EPPPAPLFEPGEL SWSFYRAGIAEFIATFLFLYI+VLTVMGV ++   C++V IQGIAWAFGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVRVGANKFPERQPIGTSAQSTD-KDYKEPPPAPLFEPGELSSWSFYRAGIAEFIATFLFLYITVLTVMGVKRAPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVF 135

HSP 2 Score: 114.775 bits (286), Expect = 3.689e-88
Identity = 58/72 (80.56%), Postives = 61/72 (84.72%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            +FYM+MQCLGAICGAGVVKGF     Y  L GGA+ VAHGYTKG GLGAEIIGTFVLVY VFSATDAKRSAR
Sbjct:  134 VFYMIMQCLGAICGAGVVKGFQPT-PYQTLGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRSAR 204
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP15_ARATH (Probable aquaporin PIP1-5 OS=Arabidopsis thaliana GN=PIP1-5 PE=2 SV=2)

HSP 1 Score: 231.106 bits (588), Expect = 3.689e-88
Identity = 114/136 (83.82%), Postives = 122/136 (89.71%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQTQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDV +GANKF ERQPIGTAAQT+  KDY EPPPAP FEPGELKSWSFYRAGIAEFIATFLFLY++VLTVMGV ++   C++V IQGIAWAFGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVNVGANKFPERQPIGTAAQTES-KDYKEPPPAPFFEPGELKSWSFYRAGIAEFIATFLFLYVTVLTVMGVKRAPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALF 135

HSP 2 Score: 115.546 bits (288), Expect = 3.689e-88
Identity = 59/72 (81.94%), Postives = 62/72 (86.11%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            LFY+VMQCLGAICGAGVVKGF   G Y    GGA+VVAHGYTKG GLGAEI+GTFVLVY VFSATDAKRSAR
Sbjct:  134 LFYIVMQCLGAICGAGVVKGFQ-PGLYQTNGGGANVVAHGYTKGSGLGAEIVGTFVLVYTVFSATDAKRSAR 204
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP15_MAIZE (Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 8.188e-88
Identity = 114/136 (83.82%), Postives = 124/136 (91.18%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQTQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVRLGAN+++ERQPIGTAAQ  + KDY EPPPAPLFE  EL SWSFYRAGIAEF+ATFLFLYIS+LTVMGV KS++KC+TV IQGIAW+FGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVRLGANRYSERQPIGTAAQGTEEKDYKEPPPAPLFEAEELTSWSFYRAGIAEFVATFLFLYISILTVMGVSKSSSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALF 136

HSP 2 Score: 111.694 bits (278), Expect = 8.188e-88
Identity = 58/72 (80.56%), Postives = 60/72 (83.33%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            LFYMVMQCLGAICGAGVVKGF   G Y    GGA+ V  GYTKGDGLGAEI+GTFVLVY VFSATDAKRSAR
Sbjct:  135 LFYMVMQCLGAICGAGVVKGFQ-EGLYMGAGGGANAVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRSAR 205
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP1_SOLLC (Probable aquaporin PIP-type pTOM75 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 1.818e-87
Identity = 110/135 (81.48%), Postives = 121/135 (89.63%), Query Frame = 3 
Query:   84 EGKEEDVRLGANKFAERQPIGTAAQTQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            E KEEDV+LGANKF E QP+GTAAQT   KDY EPPPAPLFEPGEL SWSFYRAGIAEF+ATFLFLYI++LTVMG+ +S++ CS+V IQG+AWAFGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    3 ENKEEDVKLGANKFRETQPLGTAAQTD--KDYKEPPPAPLFEPGELSSWSFYRAGIAEFMATFLFLYITILTVMGLKRSDSLCSSVGIQGVAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVF 135

HSP 2 Score: 119.013 bits (297), Expect = 1.818e-87
Identity = 61/72 (84.72%), Postives = 64/72 (88.89%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            +FYMVMQCLGAICGAGVVKGFM  G Y RL GGA+VV  GYTKGDGLGAEIIGTFVLVY VFSATDAKR+AR
Sbjct:  134 VFYMVMQCLGAICGAGVVKGFM-VGPYQRLGGGANVVNPGYTKGDGLGAEIIGTFVLVYTVFSATDAKRNAR 204
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP13_ARATH (Aquaporin PIP1-3 OS=Arabidopsis thaliana GN=PIP1-3 PE=1 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 3.093e-87
Identity = 114/136 (83.82%), Postives = 121/136 (88.97%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQTQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVR+GANKF ERQPIGT+AQT   KDY EPPPAP FEPGEL SWSFYRAGIAEFIATFLFLYI+VLTVMGV ++   C++V IQGIAWAFGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA F
Sbjct:    1 MEGKEEDVRVGANKFPERQPIGTSAQTD--KDYKEPPPAPFFEPGELSSWSFYRAGIAEFIATFLFLYITVLTVMGVKRAPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVF 134

HSP 2 Score: 114.005 bits (284), Expect = 3.093e-87
Identity = 58/72 (80.56%), Postives = 61/72 (84.72%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            +FY+VMQCLGAICGAGVVKGF     Y  L GGA+ VAHGYTKG GLGAEIIGTFVLVY VFSATDAKRSAR
Sbjct:  133 VFYIVMQCLGAICGAGVVKGFQ-PNPYQTLGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRSAR 203
[Close]
BLAST of FC925768 vs. ExPASy Swiss-Prot
Match: PIP12_ARATH (Aquaporin PIP1-2 OS=Arabidopsis thaliana GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 3.383e-86
Identity = 113/136 (83.09%), Postives = 122/136 (89.71%), Query Frame = 3 
Query:   81 MEGKEEDVRLGANKFAERQPIGTAAQTQDGKDYVEPPPAPLFEPGELKSWSFYRAGIAEFIATFLFLYISVLTVMGVVKSNTKCSTVRIQGIAWAFGGMIFVLVYCTAGISGGHINPAVTLGLFLARKLSLTRAPF 488
            MEGKEEDVR+GANKF ERQPIGT+AQ+   KDY EPPPAPLFEPGEL SWSF+RAGIAEFIATFLFLYI+VLTVMGV +S   C++V IQGIAWAFGGMIF LVYCTAGISGGHINPAVT GLFLARKLSLTRA +
Sbjct:    1 MEGKEEDVRVGANKFPERQPIGTSAQSD--KDYKEPPPAPLFEPGELASWSFWRAGIAEFIATFLFLYITVLTVMGVKRSPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVY 134

HSP 2 Score: 112.849 bits (281), Expect = 3.383e-86
Identity = 56/72 (77.78%), Postives = 62/72 (86.11%), Query Frame = 1 
Query:  484 LFYMVMQCLGAICGAGVVKGFMGAGQYGRLPGGAHVVAHGYTKGDGLGAEIIGTFVLVYPVFSATDAKRSAR 699
            ++Y+VMQCLGAICGAGVVKGF    QY  L GGA+ +AHGYTKG GLGAEIIGTFVLVY VFSATDAKR+AR
Sbjct:  133 VYYIVMQCLGAICGAGVVKGFQPK-QYQALGGGANTIAHGYTKGSGLGAEIIGTFVLVYTVFSATDAKRNAR 203
The following BLAST results are available for this feature:
BLAST of FC925768 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 67
Match NameE-valueIdentityDescription
PIP12_ORYSJ2.160e-9187.50Probable aquaporin PIP1-2 OS=Oryza sativa subsp. j... [more]
PIP12_MAIZE8.927e-9086.86Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1[more]
PIP13_MAIZE2.585e-8985.71Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2... [more]
PIP11_ORYSJ3.372e-8985.40Aquaporin PIP1-1 OS=Oryza sativa subsp. japonica G... [more]
PIP14_ARATH3.689e-8884.56Probable aquaporin PIP1-4 OS=Arabidopsis thaliana ... [more]
PIP15_ARATH3.689e-8883.82Probable aquaporin PIP1-5 OS=Arabidopsis thaliana ... [more]
PIP15_MAIZE8.188e-8883.82Aquaporin PIP1-5 OS=Zea mays GN=PIP1-5 PE=2 SV=1[more]
PIP1_SOLLC1.818e-8781.48Probable aquaporin PIP-type pTOM75 OS=Solanum lyco... [more]
PIP13_ARATH3.093e-8783.82Aquaporin PIP1-3 OS=Arabidopsis thaliana GN=PIP1-3... [more]
PIP12_ARATH3.383e-8683.09Aquaporin PIP1-2 OS=Arabidopsis thaliana GN=PIP1-2... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAP11YK10FM1 TF Citrus clementina cDNA clone KN0AAP11YK10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC925768 ID=FC925768; Name=FC925768; organism=Citrus clementina; type=EST; length=700bp
CCTCACAACCTCCAACGGCCACAAACAAAAAAGCAAGTGCTTCTTATTGA
CTGTTTTGTGTTTTTGAGGGACTAGCAGAAATGGAAGGTAAAGAAGAGGA
TGTTAGACTGGGAGCCAACAAGTTCGCCGAGAGGCAGCCGATTGGGACGG
CGGCTCAGACCCAAGATGGGAAGGACTACGTTGAGCCCCCACCGGCGCCG
CTCTTTGAGCCCGGCGAGCTCAAGTCTTGGTCGTTTTACAGGGCCGGTAT
AGCCGAGTTCATAGCCACTTTTCTTTTCTTGTACATCTCCGTACTGACAG
TGATGGGCGTCGTCAAGTCCAATACCAAGTGCTCAACCGTCCGCATTCAA
GGGATTGCATGGGCCTTCGGTGGCATGATCTTCGTGCTCGTTTACTGCAC
AGCTGGCATCTCGGGTGGTCATATCAACCCGGCGGTGACACTCGGCCTGT
TCTTGGCGAGGAAGCTGTCGCTGACACGTGCACCTTTTCTACATGGTGAT
GCAGTGTCTGGGAGCCATCTGCGGTGCTGGCGTTGTAAAGGGTTTCATGG
GTGCCGGCCAATACGGGCGGCTCCCCGGCGGTGCCCACGTCGTAGCTCAT
GGCTACACCAAGGGTGATGGTCTTGGTGCCGAGATTATTGGTACTTTTGT
TCTTGTCTACCCAGTTTTCTCCGCCACTGATGCCAAACGGAGCGCCCGGG
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