FC931003

Overview
NameFC931003
Unique NameFC931003
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length696
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: IDE_HUMAN (Insulin-degrading enzyme OS=Homo sapiens GN=IDE PE=1 SV=4)

HSP 1 Score: 68.1662 bits (165), Expect = 7.899e-24
Identity = 45/135 (33.33%), Postives = 66/135 (48.89%), Query Frame = 2 
Query:   11 EVLPHLEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGYI 415
            E L  +    L  F+P +LSR  +E  + GNI    A  ++Q +ED   + ++   +PL PSQ +  R V+L     +VY  +  N    N  +  Y Q        N+ L+L   I  +P F+ LRT EQLGYI
Sbjct:  702 EALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHT--KPLLPSQLVRYREVQLPDRGWFVYQQR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYI 832

HSP 2 Score: 62.7734 bits (151), Expect = 7.899e-24
Identity = 33/79 (41.77%), Postives = 46/79 (58.23%), Query Frame = 1 
Query:  460 GIHGVQFIIQSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            GI G++FIIQS  K P Y++ RVE+FL   E  + +MT + F+ ++ AL   +L+K K L  E   YW EI      FD
Sbjct:  842 GIQGLRFIIQSE-KPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFD 919
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: IDE_BOVIN (Insulin-degrading enzyme OS=Bos taurus GN=IDE PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 7.899e-24
Identity = 45/135 (33.33%), Postives = 66/135 (48.89%), Query Frame = 2 
Query:   11 EVLPHLEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGYI 415
            E L  +    L  F+P +LSR  +E  + GNI    A  ++Q +ED   + ++   +PL PSQ +  R V+L     +VY  +  N    N  +  Y Q        N+ L+L   I  +P F+ LRT EQLGYI
Sbjct:  702 EALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHT--KPLLPSQLVRYREVQLPDRGWFVYQQR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYI 832

HSP 2 Score: 62.7734 bits (151), Expect = 7.899e-24
Identity = 33/79 (41.77%), Postives = 46/79 (58.23%), Query Frame = 1 
Query:  460 GIHGVQFIIQSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            GI G++FIIQS  K P Y++ RVE+FL   E  + +MT + F+ ++ AL   +L+K K L  E   YW EI      FD
Sbjct:  842 GIQGLRFIIQSE-KPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFD 919
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: IDE_RAT (Insulin-degrading enzyme OS=Rattus norvegicus GN=Ide PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.264e-23
Identity = 45/135 (33.33%), Postives = 66/135 (48.89%), Query Frame = 2 
Query:   11 EVLPHLEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGYI 415
            E L  +    L  F+P +LSR  +E  + GNI    A  ++Q +ED   + ++   +PL PSQ +  R V+L     +VY  +  N    N  +  Y Q        N+ L+L   I  +P F+ LRT EQLGYI
Sbjct:  702 EALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHT--KPLLPSQLVRYREVQLPDRGWFVYQRR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYI 832

HSP 2 Score: 61.2326 bits (147), Expect = 2.264e-23
Identity = 32/79 (40.51%), Postives = 46/79 (58.23%), Query Frame = 1 
Query:  460 GIHGVQFIIQSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            GI G++FIIQS  K P Y++ RVE+FL   E  + +MT + F+ ++ AL   +L+K K L  E   YW EI      +D
Sbjct:  842 GIQGLRFIIQSE-KPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYD 919
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: IDE_MOUSE (Insulin-degrading enzyme OS=Mus musculus GN=Ide PE=2 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.264e-23
Identity = 45/135 (33.33%), Postives = 66/135 (48.89%), Query Frame = 2 
Query:   11 EVLPHLEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGYI 415
            E L  +    L  F+P +LSR  +E  + GNI    A  ++Q +ED   + ++   +PL PSQ +  R V+L     +VY  +  N    N  +  Y Q        N+ L+L   I  +P F+ LRT EQLGYI
Sbjct:  702 EALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHT--KPLLPSQLVRYREVQLPDRGWFVYQQR--NEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYI 832

HSP 2 Score: 61.2326 bits (147), Expect = 2.264e-23
Identity = 32/79 (40.51%), Postives = 46/79 (58.23%), Query Frame = 1 
Query:  460 GIHGVQFIIQSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            GI G++FIIQS  K P Y++ RVE+FL   E  + +MT + F+ ++ AL   +L+K K L  E   YW EI      +D
Sbjct:  842 GIQGLRFIIQSE-KPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYD 919
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: IDE_DROME (Insulin-degrading enzyme OS=Drosophila melanogaster GN=Ide PE=1 SV=4)

HSP 1 Score: 56.9954 bits (136), Expect = 1.501e-17
Identity = 36/138 (26.09%), Postives = 64/138 (46.38%), Query Frame = 2 
Query:    2 EELEVLPHLEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGYI 415
            E L+ +  +  + +  F      R   EC+I GN+   +A  +   + +   + +N    P+   Q L  R  KL  G +Y++  +  N   ++SC   Y+Q        N+ + L++ +  +P +  LRT EQLGYI
Sbjct:  672 ELLDAMELVTYDRVLNFAKEFFQRLHTECFIFGNVTKQQATDIAGRV-NTRLEATNASKLPILARQMLKKREYKLLAGDSYLFEKE--NEFHKSSCAQLYLQCGAQTDHTNIMVNLVSQVLSEPCYDCLRTKEQLGYI 806

HSP 2 Score: 52.7582 bits (125), Expect = 1.501e-17
Identity = 27/79 (34.18%), Postives = 49/79 (62.03%), Query Frame = 1 
Query:  460 GIHGVQFIIQSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            G +G++ I+QS+ K P Y++ R+E+FLQ +   + +M  D+F+ +  AL   KLEK K + ++   ++ EI+  T  F+
Sbjct:  816 GANGIRIIVQSA-KHPSYVEDRIENFLQTYLQVIEDMPLDEFERHKEALAVKKLEKPKTIFQQFSQFYGEIAMQTYHFE 893
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: STE23_YEAST (A-factor-processing enzyme OS=Saccharomyces cerevisiae GN=STE23 PE=1 SV=2)

HSP 1 Score: 56.9954 bits (136), Expect = 1.293e-15
Identity = 42/138 (30.43%), Postives = 62/138 (44.93%), Query Frame = 2 
Query:    2 EELEVLPHLEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGYI 415
            E+L+V   L  E L  F+P +    + E  I GNI+  EA  +   I+ +     N I      +  L  R   L KGK + Y     +  + NSC+ H  Q+      ++    L A +  +P F  LRT EQLGY+
Sbjct:  709 EKLQVFEKLTFEQLINFIPTIYEGVYFETLIHGNIKHEEALEVDSLIKSLI---PNNIHNLQVSNNRL--RSYLLPKGKTFRYETALKDSQNVNSCIQHVTQLDVYSEDLSALSGLFAQLIHEPCFDTLRTKEQLGYV 841

HSP 2 Score: 46.2098 bits (108), Expect = 1.293e-15
Identity = 26/81 (32.10%), Postives = 42/81 (51.85%), Query Frame = 1 
Query:  451 NDFGIHGVQFIIQSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKF 693
            N+ G   ++ +IQS    P Y++ R+ +F + F   L +M  + F+ +  AL +  L+K KN+ EES  Y   I  G   F
Sbjct:  848 NNHGTANIRILIQSEHTTP-YLEWRINNFYETFGQVLRDMPEEDFEKHKEALCNSLLQKFKNMAEESARYTAAIYLGDYNF 927
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: NRDC_HUMAN (Nardilysin OS=Homo sapiens GN=NRD1 PE=1 SV=2)

HSP 1 Score: 51.6026 bits (122), Expect = 6.209e-15
Identity = 39/130 (30.00%), Postives = 62/130 (47.69%), Query Frame = 2 
Query:   26 LEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIED-VFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGY 412
            L  E L  FV    S+ F+E  + GN+ S E+   ++Y+ D + FK       PL     +  +VV+L  G +++   + LN  D NS +  Y Q           ++LL +  ++P F  LRT + LGY
Sbjct:  838 LSLESLLSFVKEFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFK-------PLEQEMPVQFQVVELPSG-HHLCKVKALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGY 959

HSP 2 Score: 49.2914 bits (116), Expect = 6.209e-15
Identity = 28/86 (32.56%), Postives = 42/86 (48.84%), Query Frame = 1 
Query:  445 CRNDFGIHGVQFII--QSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            CRN  GI G    +  Q++    + +D ++E FL  FE K+  +T + F   V ALI +K  +  +L EE    W E+      FD
Sbjct:  965 CRNTSGILGFSVTVGTQATKYNSEVVDKKIEEFLSSFEEKIENLTEEAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFD 1050
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: NRDC_RAT (Nardilysin OS=Rattus norvegicus GN=Nrd1 PE=2 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 1.361e-14
Identity = 29/86 (33.72%), Postives = 42/86 (48.84%), Query Frame = 1 
Query:  445 CRNDFGIHGVQFII--QSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            CRN  GI G    +  Q++    + +D ++E FL  FE K+  +T D F   V ALI +K  +  +L EE    W E+      FD
Sbjct:  977 CRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFD 1062

HSP 2 Score: 49.2914 bits (116), Expect = 1.361e-14
Identity = 38/129 (29.46%), Postives = 61/129 (47.29%), Query Frame = 2 
Query:   26 LEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGY 412
            L  E L  FV    S+ F+E  + GN+ S E+   ++Y+ D      N +  PL     +  +VV+L  G +++   + LN  D NS +  Y Q           ++LL +  ++P F  LRT + LGY
Sbjct:  850 LSLESLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLRYVVDKL----NFV--PLEREMPVQFQVVELPSG-HHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGY 971
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: NRDC_PONAB (Nardilysin OS=Pongo abelii GN=NRD1 PE=2 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 2.298e-14
Identity = 39/130 (30.00%), Postives = 62/130 (47.69%), Query Frame = 2 
Query:   26 LEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIED-VFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGY 412
            L  E L  FV    S+ F+E  + GN+ S E+   ++Y+ D + FK       PL     +  +VV+L  G +++   + LN  D NS +  Y Q           ++LL +  ++P F  LRT + LGY
Sbjct:  840 LSLESLLSFVKEFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFK-------PLEQEMPVQFQVVELPSG-HHLCKVKALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGY 961

HSP 2 Score: 47.3654 bits (111), Expect = 2.298e-14
Identity = 27/86 (31.40%), Postives = 42/86 (48.84%), Query Frame = 1 
Query:  445 CRNDFGIHGVQFII--QSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            CR+  GI G    +  Q++    + +D ++E FL  FE K+  +T + F   V ALI +K  +  +L EE    W E+      FD
Sbjct:  967 CRSTSGILGFSVTVGTQATKYNSEVVDKKIEEFLSSFEEKIENLTEEAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFD 1052
[Close]
BLAST of FC931003 vs. ExPASy Swiss-Prot
Match: NRDC_MOUSE (Nardilysin OS=Mus musculus GN=Nrd1 PE=1 SV=1)

HSP 1 Score: 50.447 bits (119), Expect = 2.984e-14
Identity = 29/86 (33.72%), Postives = 42/86 (48.84%), Query Frame = 1 
Query:  445 CRNDFGIHGVQFII--QSSVKGPKYIDLRVESFLQMFESKLYEMTSDQFKNNVNALIDMKLEKHKNLKEESGFYWREISDGTLKFD 696
            CRN  GI G    +  Q++    + +D ++E FL  FE K+  +T D F   V ALI +K  +  +L EE    W E+      FD
Sbjct:  977 CRNTSGILGFSVTVGTQATKYNSETVDKKIEEFLSSFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFD 1062

HSP 2 Score: 48.1358 bits (113), Expect = 2.984e-14
Identity = 36/129 (27.91%), Postives = 59/129 (45.74%), Query Frame = 2 
Query:   26 LEAEDLAKFVPMMLSRTFLECYIAGNIESNEAGSMIQYIEDVFFKGSNPICQPLFPSQHLTNRVVKLEKGKNYVYSNQGLNPSDENSCLVHYIQVHRDDFLMNVKLQLLALIAKQPAFHQLRTVEQLGY 412
            L  + L  FV    S+ F+E  + GN+ S E+   ++Y+ D           PL     +  +VV+L  G +++   + LN  D NS +  Y Q           ++LL +  ++P F  LRT + LGY
Sbjct:  850 LSLDSLLNFVKDFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFA------PLEREMPVQFQVVELPSG-HHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGY 971
The following BLAST results are available for this feature:
BLAST of FC931003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
IDE_HUMAN7.899e-2433.33Insulin-degrading enzyme OS=Homo sapiens GN=IDE PE... [more]
IDE_BOVIN7.899e-2433.33Insulin-degrading enzyme OS=Bos taurus GN=IDE PE=2... [more]
IDE_RAT2.264e-2333.33Insulin-degrading enzyme OS=Rattus norvegicus GN=I... [more]
IDE_MOUSE2.264e-2333.33Insulin-degrading enzyme OS=Mus musculus GN=Ide PE... [more]
IDE_DROME1.501e-1726.09Insulin-degrading enzyme OS=Drosophila melanogaste... [more]
STE23_YEAST1.293e-1530.43A-factor-processing enzyme OS=Saccharomyces cerevi... [more]
NRDC_HUMAN6.209e-1530.00Nardilysin OS=Homo sapiens GN=NRD1 PE=1 SV=2[more]
NRDC_RAT1.361e-1433.72Nardilysin OS=Rattus norvegicus GN=Nrd1 PE=2 SV=1[more]
NRDC_PONAB2.298e-1430.00Nardilysin OS=Pongo abelii GN=NRD1 PE=2 SV=1[more]
NRDC_MOUSE2.984e-1433.72Nardilysin OS=Mus musculus GN=Nrd1 PE=1 SV=1[more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC34100D03EF PostHarvP1 Citrus clementina cDNA clone C34100D03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931003 ID=FC931003; Name=FC931003; organism=Citrus clementina; type=EST; length=696bp
GGAAGAACTTGAAGTTCTTCCCCATCTTGAAGCTGAAGATCTTGCTAAAT
TTGTACCCATGATGCTCTCTAGGACCTTCTTGGAATGTTACATAGCAGGA
AACATTGAATCTAATGAAGCAGGATCGATGATCCAGTACATTGAAGATGT
TTTCTTTAAGGGCTCAAACCCAATATGCCAACCATTGTTCCCATCCCAGC
ATTTGACTAATAGAGTTGTTAAACTTGAAAAGGGCAAGAATTATGTGTAC
TCCAACCAAGGCCTGAATCCTAGTGATGAAAATTCTTGCCTAGTCCATTA
TATTCAGGTTCATCGAGATGATTTCCTTATGAATGTGAAACTTCAGCTTC
TTGCTCTTATTGCAAAACAACCAGCTTTCCACCAGCTTAGAACCGTTGAG
CAACTTGGGTACATTACAGCACTCCTGCGGAGCTATCTACTTGATGTAGG
AATGATTTTGGTATCCATGGAGTGCAGTTCATCATTCAATCCTCAGTAAA
GGGTCCGAAGTATATTGATCTGAGAGTTGAATCATTCCTCCAGATGTTTG
AAAGTAAACTTTATGAGATGACCAGTGACCAATTCAAGAACAACGTAAAT
GCATTGATTGATATGAAGCTTGAGAAACACAAGAATTTAAAGGAAGAATC
TGGATTTTACTGGCGTGAGATTTCTGATGGGACCCTCAAGTTTGAT
back to top