FC931056

Overview
NameFC931056
Unique NameFC931056
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length736
Libraries
Library NameType
PostHarvP1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig1971 contig Ccv1_Contig1971:60..797. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 9.571e-48
Identity = 79/102 (77.45%), Postives = 91/102 (89.22%), Query Frame = 3 
Query:  351 LEWVKKDKRRMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            +EW KKDKRR LHVVYRVGDLDRTI+FYTEC GMK+LRKRD+PEEKY+NAFLG+GPE S+FV+ELTYNYGV  YDIGTGFGHF I+  DV+K VE ++AKGG
Sbjct:    8 VEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGG 109

HSP 2 Score: 32.7278 bits (73), Expect = 9.571e-48
Identity = 12/18 (66.67%), Postives = 16/18 (88.89%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYRF 735
            +GG +VIAF++DPDGY F
Sbjct:  119 KGGGSVIAFVKDPDGYTF 136

HSP 3 Score: 22.7126 bits (47), Expect = 9.571e-48
Identity = 8/9 (88.89%), Postives = 9/9 (100.00%), Query Frame = 2 
Query:  659 ITREPGPVK 685
            +TREPGPVK
Sbjct:  111 VTREPGPVK 119

HSP 4 Score: 80.4925 bits (197), Expect = 1.040e-14
Identity = 40/88 (45.45%), Postives = 54/88 (61.36%), Query Frame = 3 
Query:  381 MLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIK 644
            +  V+ RVGDLDR +KF  + LGM+LLR+ + PE   T   +GY  E    V+ELTYNYGV +Y  G  +    I  DDV K+ E++K
Sbjct:  148 LCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVK 234
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 172.17 bits (435), Expect = 3.562e-47
Identity = 78/99 (78.79%), Postives = 87/99 (87.88%), Query Frame = 3 
Query:  351 LEWVKKDKRRMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKA 647
            LEW KKDK+R+LH VYRVGDLDRTIK YTEC GMKLLRKRD+PEEKYTNAFLG+GPED++F +ELTYNYGVDKYDIG GFGHF IA +DV K  E IK+
Sbjct:   15 LEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKS 113

HSP 2 Score: 33.4982 bits (75), Expect = 3.562e-47
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYRF 735
            +GG+TVIAF +DPDGY F
Sbjct:  127 KGGSTVIAFAQDPDGYMF 144

HSP 3 Score: 25.0238 bits (53), Expect = 3.562e-47
Identity = 10/10 (100.00%), Postives = 10/10 (100.00%), Query Frame = 2 
Query:  656 KITREPGPVK 685
            KITREPGPVK
Sbjct:  118 KITREPGPVK 127

HSP 4 Score: 102.834 bits (255), Expect = 1.957e-21
Identity = 52/97 (53.61%), Postives = 64/97 (65.98%), Query Frame = 3 
Query:  381 MLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELI----KAKGGK 659
            +  V+ RVGDLDR+IKFY + LGMKLLRK+D+P+ KYT A LGY  ED   VIELTYNYGV +Y  G  +    I  +DV K+ E +    K  GGK
Sbjct:  156 LCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGK 252
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.803e-30
Identity = 54/93 (58.06%), Postives = 70/93 (75.27%), Query Frame = 3 
Query:  378 RMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            R+LH + RVGDL R+I FYT+ LGMKLLR  + PE KY+ AF+GYGPE    VIELTYN+GVDKY++GT +GH  ++VD+ A+  E I+  GG
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGG 94

HSP 2 Score: 34.6538 bits (78), Expect = 3.803e-30
Identity = 13/17 (76.47%), Postives = 16/17 (94.12%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYR 732
            +GG TVIAF+EDPDGY+
Sbjct:  104 KGGTTVIAFVEDPDGYK 120
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.803e-30
Identity = 54/93 (58.06%), Postives = 70/93 (75.27%), Query Frame = 3 
Query:  378 RMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            R+LH + RVGDL R+I FYT+ LGMKLLR  + PE KY+ AF+GYGPE    VIELTYN+GVDKY++GT +GH  ++VD+ A+  E I+  GG
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGG 94

HSP 2 Score: 34.6538 bits (78), Expect = 3.803e-30
Identity = 13/17 (76.47%), Postives = 16/17 (94.12%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYR 732
            +GG TVIAF+EDPDGY+
Sbjct:  104 KGGTTVIAFVEDPDGYK 120
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 118.242 bits (295), Expect = 3.803e-30
Identity = 54/93 (58.06%), Postives = 70/93 (75.27%), Query Frame = 3 
Query:  378 RMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            R+LH + RVGDL R+I FYT+ LGMKLLR  + PE KY+ AF+GYGPE    VIELTYN+GVDKY++GT +GH  ++VD+ A+  E I+  GG
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGG 94

HSP 2 Score: 34.6538 bits (78), Expect = 3.803e-30
Identity = 13/17 (76.47%), Postives = 16/17 (94.12%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYR 732
            +GG TVIAF+EDPDGY+
Sbjct:  104 KGGTTVIAFVEDPDGYK 120
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 4.783e-29
Identity = 50/93 (53.76%), Postives = 70/93 (75.27%), Query Frame = 3 
Query:  381 MLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGGK 659
            +LH + RVGDLD++++FY + LGM LLRK+D P  ++T AF+GYG E  + VIELT+N+G DKYD+G GFGH  + V+D+  T + I+ KGGK
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKGGK 95

HSP 2 Score: 31.9574 bits (71), Expect = 4.783e-29
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:  688 GNTVIAFIEDPDGYR 732
            G TVIAF+EDPDGY+
Sbjct:  106 GTTVIAFVEDPDGYK 120

HSP 3 Score: 21.557 bits (44), Expect = 4.783e-29
Identity = 7/10 (70.00%), Postives = 9/10 (90.00%), Query Frame = 2 
Query:  656 KITREPGPVK 685
            K+ REPGP+K
Sbjct:   95 KVVREPGPMK 104
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_HAEIN (Lactoylglutathione lyase OS=Haemophilus influenzae GN=gloA PE=1 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.939e-29
Identity = 54/93 (58.06%), Postives = 68/93 (73.12%), Query Frame = 3 
Query:  378 RMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            ++LH + RVGDLDR+IKFY + LGM+LLR  + PE KYT AFLGY   +S   IELTYN+GVDKY+ GT +GH  I VDD+  T E ++A GG
Sbjct:    2 QILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIELTYNWGVDKYEHGTAYGHIAIGVDDIYATCEAVRASGG 94

HSP 2 Score: 35.039 bits (79), Expect = 6.939e-29
Identity = 13/17 (76.47%), Postives = 17/17 (100.00%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYR 732
            +GG+TVIAF+EDPDGY+
Sbjct:  104 KGGSTVIAFVEDPDGYK 120
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 9.035e-29
Identity = 52/93 (55.91%), Postives = 67/93 (72.04%), Query Frame = 3 
Query:  378 RMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            R+LH + RVGDL R+I FYT  LGMKLLR  + PE KY+ AF+GYGPE    VIELTYN+GV+ YD+G  +GH  ++VD+ A+  E I+  GG
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGG 94

HSP 2 Score: 34.6538 bits (78), Expect = 9.035e-29
Identity = 12/17 (70.59%), Postives = 17/17 (100.00%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYR 732
            +GG+T+IAF+EDPDGY+
Sbjct:  104 KGGSTIIAFVEDPDGYK 120
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_SALTI (Lactoylglutathione lyase OS=Salmonella typhi GN=gloA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 9.035e-29
Identity = 52/93 (55.91%), Postives = 67/93 (72.04%), Query Frame = 3 
Query:  378 RMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            R+LH + RVGDL R+I FYT  LGMKLLR  + PE KY+ AF+GYGPE    VIELTYN+GV+ YD+G  +GH  ++VD+ A+  E I+  GG
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGG 94

HSP 2 Score: 34.6538 bits (78), Expect = 9.035e-29
Identity = 12/17 (70.59%), Postives = 17/17 (100.00%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGYR 732
            +GG+T+IAF+EDPDGY+
Sbjct:  104 KGGSTIIAFVEDPDGYK 120
[Close]
BLAST of FC931056 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 114.775 bits (286), Expect = 1.263e-27
Identity = 54/93 (58.06%), Postives = 68/93 (73.12%), Query Frame = 3 
Query:  378 RMLHVVYRVGDLDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGTGFGHFGIAVDDVAKTVELIKAKGG 656
            R+LH + RVGDLD++IKFYTE +GM+LLR  +  E +YT AF+GYG E    VIELTYN+G  +YD+GT FGH  I VDD+  T + IKA GG
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGG 97

HSP 2 Score: 29.6462 bits (65), Expect = 1.263e-27
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 1 
Query:  682 QGGNTVIAFIEDPDGY 729
            +GG T IAF++DPDGY
Sbjct:  107 KGGTTHIAFVKDPDGY 122
The following BLAST results are available for this feature:
BLAST of FC931056 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 18
Match NameE-valueIdentityDescription
LGUL_BRAOG9.571e-4877.45Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_ORYSJ3.562e-4778.79Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_SHIFL3.803e-3058.06Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI3.803e-3058.06Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO573.803e-3058.06Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_SYNY34.783e-2953.76Probable lactoylglutathione lyase OS=Synechocystis... [more]
LGUL_HAEIN6.939e-2958.06Lactoylglutathione lyase OS=Haemophilus influenzae... [more]
LGUL_SALTY9.035e-2955.91Lactoylglutathione lyase OS=Salmonella typhimurium... [more]
LGUL_SALTI9.035e-2955.91Lactoylglutathione lyase OS=Salmonella typhi GN=gl... [more]
LGUL_VIBPA1.263e-2758.06Probable lactoylglutathione lyase OS=Vibrio paraha... [more]

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Properties
Property NameValue
Genbank descriptionC34100H10EF PostHarvP1 Citrus clementina cDNA clone C34100H10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC931056 ID=FC931056; Name=FC931056; organism=Citrus clementina; type=EST; length=736bp
CTTGGCCGGGAGAAGCTGAGGGAGTGAGTGCAGTGAGCTGAGTGAAGGCG
AGCGAGAATCAATAAGTAAAACAATAGGAGATGGTAAGGATAATATCAAT
GGCGTCCTCAATTCGGCCTTCACTTTCCACCTTCAGATTCTCTTCCTCCT
CCCGTTTTGGACTCCCTCTCTCCTCCTTTACTCCTTCACGCAACCTCGTT
TTCTCTCCCCTTGCCAGCGCTGTCCCGCAGTCGCAATTATTCGGTCTGAG
AGCTGCTAAGCCCTTGAGAGGAGATGTGAACAGCACAGGGGTGGCGTCGT
CGGCTGGGAATGCAGCGCAGGCAAGCACGAGTGCTGCTCATGAAAGTGCC
CTGGAATGGGTCAAGAAAGACAAAAGAAGAATGCTTCATGTTGTTTATCG
TGTTGGAGATTTGGACAGGACGATTAAATTCTACACTGAGTGCCTCGGGA
TGAAGTTGCTCCGTAAACGTGACATACCAGAGGAGAAATATACAAATGCG
TTTCTTGGATACGGACCAGAAGATTCTCACTTTGTTATTGAACTCACTTA
CAATTATGGAGTTGACAAGTATGATATCGGAACTGGATTTGGCCATTTCG
GTATTGCTGTGGATGATGTTGCCAAGACTGTTGAACTCATAAAGGCTAAA
GGAGGAAAATAACCCGAGAACCTGGTCCTGTCAAGGTGGCAATACTGTGA
TTGCATTTATTGAAGATCCTGATGGNTACAGATTTG
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