FC932553

Overview
NameFC932553
Unique NameFC932553
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length739
Libraries
Library NameType
PostHarveNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Ccv1_Contig14122 contig Ccv1_Contig14122:7..746. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 338.961 bits (868), Expect = 1.635e-92
Identity = 163/209 (77.99%), Postives = 184/209 (88.04%), Query Frame = 3 
Query:  111 NAELLEWPKKDKRRLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEYT 737
            NA+L+EWPKKDKRR LH VYRVGDLDRTI++YTECFGM++LRKRDVPEEKYSNAFLGFGPE S FVVELTYNYGV+SYDIGTGFGH AI+T+DV KMVE +RAKGGNVTREPGP+KG  +  AFVKDPDGY FELIQRGPTPEPLCQVMLRVGDL R++KF EKALGM+LLR I+ PE    + M+GYAEE ++ VLEL Y+Y VTEYT
Sbjct:    4 NADLVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYT 211

HSP 2 Score: 100.523 bits (249), Expect = 9.787e-21
Identity = 52/121 (42.98%), Postives = 70/121 (57.85%), Query Frame = 3 
Query:  153 LLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIR----AKGGNVTREPGPLKGMTTHFAFVKDPDGY 503
            L   + RVGDLDR +K+  +  GM LLR+ + PE   +   +G+  E    V+ELTYNYGVT Y  G  +  +AI T+DVYK  E ++      GG +TRE GPL G+ T      DPDG+
Sbjct:  148 LCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGW 267
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 333.183 bits (853), Expect = 8.972e-91
Identity = 164/218 (75.23%), Postives = 184/218 (84.40%), Query Frame = 3 
Query:   87 AEASPAVANAELLEWPKKDKRRLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAK-GGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEYT 737
            AE SP V    +LEWPKKDK+RLLHAVYRVGDLDRTIK YTECFGM+LLRKRDVPEEKY+NAFLGFGPE + F +ELTYNYGV  YDIG GFGH AIATEDVYK+ E I++     +TREPGP+KG +T  AF +DPDGY+FELIQRGPTPEPLCQVMLRVGDL RSIKFYEKALGMKLLR  D P+ K  +AMLGYA+ED+TTV+EL Y+Y VTEYT
Sbjct:    7 AEKSPEV----VLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYT 220

HSP 2 Score: 126.331 bits (316), Expect = 1.666e-28
Identity = 62/126 (49.21%), Postives = 81/126 (64.29%), Query Frame = 3 
Query:  153 LLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENI----RAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELI 518
            L   + RVGDLDR+IK+Y +  GM+LLRK+DVP+ KY+ A LG+  E    V+ELTYNYGVT Y  G  +  +AI TEDVYK  E +    +  GG + R+PGPL G+ T  A   DPDG+   L+
Sbjct:  156 LCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLV 281
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 162.155 bits (409), Expect = 2.739e-39
Identity = 74/124 (59.68%), Postives = 95/124 (76.61%), Query Frame = 3 
Query:  150 RLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            R+LH + RVGDLD++IK+YTE  GM+LLR  +  E +Y+ AF+G+G E    V+ELTYN+G T YD+GT FGH+AI  +D+Y   + I+A GGNVTRE GP+KG TTH AFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 69.3218 bits (168), Expect = 2.416e-11
Identity = 32/64 (50.00%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:  543 LCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEY 734
            +   MLRVGDL +SIKFY + +GM+LLRT ++ E +  LA +GY +E Q  V+EL Y++  TEY
Sbjct:    6 ILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEY 69
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 157.918 bits (398), Expect = 5.166e-38
Identity = 72/124 (58.06%), Postives = 93/124 (75.00%), Query Frame = 3 
Query:  150 RLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            R+LH + RVGDLD++I++YT+  GM LLRK +  E KY+ AFLG+G E    V+ELTYN+GV  Y+ G  +GH+AI  +D+Y   + I+A GG VTREPGP+KG TTH AFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQ 128
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.077e-35
Identity = 70/124 (56.45%), Postives = 92/124 (74.19%), Query Frame = 3 
Query:  150 RLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            RLLH + RVGDL R+I +YT+  GM+LLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH+A++ ++  +  E IR  GGNVTRE GP+KG TT  AFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 73.9442 bits (180), Expect = 9.810e-13
Identity = 36/64 (56.25%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:  543 LCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEY 734
            L   MLRVGDL RSI FY K LGMKLLRT ++PE K +LA +GY  E +  V+EL Y++ V +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.077e-35
Identity = 70/124 (56.45%), Postives = 92/124 (74.19%), Query Frame = 3 
Query:  150 RLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            RLLH + RVGDL R+I +YT+  GM+LLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH+A++ ++  +  E IR  GGNVTRE GP+KG TT  AFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 73.9442 bits (180), Expect = 9.810e-13
Identity = 36/64 (56.25%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:  543 LCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEY 734
            L   MLRVGDL RSI FY K LGMKLLRT ++PE K +LA +GY  E +  V+EL Y++ V +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.077e-35
Identity = 70/124 (56.45%), Postives = 92/124 (74.19%), Query Frame = 3 
Query:  150 RLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            RLLH + RVGDL R+I +YT+  GM+LLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH+A++ ++  +  E IR  GGNVTRE GP+KG TT  AFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 73.9442 bits (180), Expect = 9.810e-13
Identity = 36/64 (56.25%), Postives = 45/64 (70.31%), Query Frame = 3 
Query:  543 LCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEY 734
            L   MLRVGDL RSI FY K LGMKLLRT ++PE K +LA +GY  E +  V+EL Y++ V +Y
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKY 66
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 2.400e-35
Identity = 70/124 (56.45%), Postives = 91/124 (73.39%), Query Frame = 3 
Query:  150 RLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            RLLH + RVGDL R+I +YT   GM+LLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH+A++ ++  +  E IR  GGNVTRE GP+KG +T  AFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 71.633 bits (174), Expect = 4.869e-12
Identity = 35/64 (54.69%), Postives = 43/64 (67.19%), Query Frame = 3 
Query:  543 LCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEY 734
            L   MLRVGDL RSI FY   LGMKLLRT ++PE K +LA +GY  E +  V+EL Y++ V  Y
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESY 66
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_SALTI (Lactoylglutathione lyase OS=Salmonella typhi GN=gloA PE=3 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 2.400e-35
Identity = 70/124 (56.45%), Postives = 91/124 (73.39%), Query Frame = 3 
Query:  150 RLLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            RLLH + RVGDL R+I +YT   GM+LLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH+A++ ++  +  E IR  GGNVTRE GP+KG +T  AFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 71.633 bits (174), Expect = 4.869e-12
Identity = 35/64 (54.69%), Postives = 43/64 (67.19%), Query Frame = 3 
Query:  543 LCQVMLRVGDLGRSIKFYEKALGMKLLRTIDSPELKCALAMLGYAEEDQTTVLELAYSYAVTEY 734
            L   MLRVGDL RSI FY   LGMKLLRT ++PE K +LA +GY  E +  V+EL Y++ V  Y
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESY 66
[Close]
BLAST of FC932553 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 4.093e-35
Identity = 66/123 (53.66%), Postives = 89/123 (72.36%), Query Frame = 3 
Query:  153 LLHAVYRVGDLDRTIKYYTECFGMELLRKRDVPEEKYSNAFLGFGPEQSYFVVELTYNYGVTSYDIGTGFGHLAIATEDVYKMVENIRAKGGNVTREPGPLKGMTTHFAFVKDPDGYIFELIQ 521
            LLH + RVGDLD+++++Y +  GM LLRK+D P  +++ AF+G+G E    V+ELT+N+G   YD+G GFGH+A+  ED+Y   + IR KGG V REPGP+K  TT  AFV+DPDGY  ELIQ
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIELIQ 125
The following BLAST results are available for this feature:
BLAST of FC932553 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 27
Match NameE-valueIdentityDescription
LGUL_BRAOG1.635e-9277.99Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_ORYSJ8.972e-9175.23Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_VIBPA2.739e-3959.68Probable lactoylglutathione lyase OS=Vibrio paraha... [more]
LGUL_VIBCH5.166e-3858.06Probable lactoylglutathione lyase OS=Vibrio choler... [more]
LGUL_SHIFL1.077e-3556.45Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI1.077e-3556.45Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO571.077e-3556.45Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_SALTY2.400e-3556.45Lactoylglutathione lyase OS=Salmonella typhimurium... [more]
LGUL_SALTI2.400e-3556.45Lactoylglutathione lyase OS=Salmonella typhi GN=gl... [more]
LGUL_SYNY34.093e-3553.66Probable lactoylglutathione lyase OS=Synechocystis... [more]

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Properties
Property NameValue
Genbank descriptionC34209H01EF PostHarveN Citrus clementina cDNA clone C34209H01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC932553 ID=FC932553; Name=FC932553; organism=Citrus clementina; type=EST; length=739bp
GAAGTCTGGAAAATAGATCATCTTCTAGATCATTTCTCACACTTGCGGCT
TCACTCCGACTTTCTGAGTGATTCCATATCAAGATGGCTGAGGCTTCTCC
TGCTGTTGCTAATGCCGAGTTGCTGGAATGGCCAAAGAAAGATAAGCGAC
GCCTCCTTCATGCTGTGTATCGTGTTGGTGATCTTGATCGCACCATCAAA
TATTACACTGAATGCTTTGGGATGGAGCTGTTGAGAAAGAGAGACGTCCC
AGAAGAGAAATACTCAAATGCCTTTCTTGGATTTGGCCCCGAACAGTCTT
ACTTTGTTGTGGAGTTAACATACAACTATGGTGTAACTTCATATGATATT
GGAACTGGTTTTGGCCATCTTGCAATTGCAACTGAAGATGTTTACAAAAT
GGTTGAAAACATTCGTGCCAAGGGTGGCAATGTGACTAGAGAGCCTGGTC
CACTTAAAGGTATGACAACTCACTTTGCCTTTGTGAAGGATCCCGATGGT
TATATTTTTGAACTCATCCAGAGAGGTCCAACTCCTGAACCACTCTGTCA
AGTAATGCTTCGCGTTGGCGATCTTGGTCGCTCCATCAAGTTCTATGAAA
AGGCTTTGGGGATGAAACTGTTAAGGACAATTGATAGTCCTGAATTGAAG
TGCGCCCTGGCCATGCTTGGATATGCTGAGGAAGATCAGACTACTGTTCT
GGAGTTAGCTTATAGTTATGCTGTGACTGAATATACCAG
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