BQ623830

Overview
NameBQ623830
Unique NameBQ623830
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length669
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig6276 contig Csv1_Contig6276:110..778. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_NICSY (Photosystem I reaction center subunit II, chloroplastic OS=Nicotiana sylvestris GN=psaD PE=2 SV=1)

HSP 1 Score: 209.534 bits (532), Expect = 1.261e-53
Identity = 105/129 (81.40%), Postives = 111/129 (86.05%), Query Frame = 1 
Query:    7 SSFSVRPLKHNAPRTNTIKAMAEGKTETPTKEAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 393
            +SFS + LK        I+AMAE +  T   EAPVGFTPP+LDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREG NLLKLARKEQCLALGTRLRSKYKI Y+FYRVFPNG
Sbjct:   28 ASFSPKQLKSTVSAPRPIRAMAE-EAATKEAEAPVGFTPPQLDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGANLLKLARKEQCLALGTRLRSKYKINYRFYRVFPNG 155

HSP 2 Score: 106.301 bits (264), Expect = 1.490e-22
Identity = 49/62 (79.03%), Postives = 54/62 (87.10%), Query Frame = 2 
Query:  356 RLSTSFTECFPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYDL 541
            +++  F   FP GEVQYLHPKDGVYPEKVN GR+GVGQN+RSIGKN SPIEVKFTGKQVYDL
Sbjct:  143 KINYRFYRVFPNGEVQYLHPKDGVYPEKVNAGRQGVGQNFRSIGKNKSPIEVKFTGKQVYDL 204
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_SOLLC (Photosystem I reaction center subunit II, chloroplastic OS=Solanum lycopersicum GN=psaD PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 3.670e-53
Identity = 106/130 (81.54%), Postives = 110/130 (84.62%), Query Frame = 1 
Query:   10 SFSV-RPLKHNAPRTNTIKAMAE-GKTETPTKEAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 393
            SFS  + LK     T  I+AMAE     T  K AP GFTPP+LDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMR+GPNLLKLARKEQCLALGTRLRSKYKI YQFYRVFPNG
Sbjct:   30 SFSTPKQLKSTVSVTRPIRAMAEEAPAATEEKPAPAGFTPPQLDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMRQGPNLLKLARKEQCLALGTRLRSKYKINYQFYRVFPNG 159

HSP 2 Score: 107.071 bits (266), Expect = 8.737e-23
Identity = 49/62 (79.03%), Postives = 54/62 (87.10%), Query Frame = 2 
Query:  356 RLSTSFTECFPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYDL 541
            +++  F   FP GEVQYLHPKDGVYPEKVNPGREGVGQN+RSIGKN S IEVKFTGKQVYD+
Sbjct:  147 KINYQFYRVFPNGEVQYLHPKDGVYPEKVNPGREGVGQNFRSIGKNKSAIEVKFTGKQVYDI 208
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_SPIOL (Photosystem I reaction center subunit II, chloroplastic OS=Spinacia oleracea GN=psaD PE=1 SV=2)

HSP 1 Score: 207.608 bits (527), Expect = 4.794e-53
Identity = 106/122 (86.89%), Postives = 110/122 (90.16%), Query Frame = 1 
Query:   40 APRTNTIKAMA--EGKTE--TPTKEAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 393
            A R ++I+A A  EGK    T TKEAP GFTPPELDPNTPSPIF GSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFP+G
Sbjct:   42 ASRHHSIRAAAAAEGKAAAATETKEAPKGFTPPELDPNTPSPIFAGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPSG 163

HSP 2 Score: 105.531 bits (262), Expect = 2.542e-22
Identity = 49/62 (79.03%), Postives = 52/62 (83.87%), Query Frame = 2 
Query:  356 RLSTSFTECFPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYDL 541
            ++   F   FP GEVQYLHPKDGVYPEKVNPGR+GVG N RSIGKNVSPIEVKFTGKQ YDL
Sbjct:  151 KIKYQFYRVFPSGEVQYLHPKDGVYPEKVNPGRQGVGLNMRSIGKNVSPIEVKFTGKQPYDL 212
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_CUCSA (Photosystem I reaction center subunit II, chloroplastic OS=Cucumis sativus GN=psaD PE=1 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 1.542e-51
Identity = 104/134 (77.61%), Postives = 111/134 (82.84%), Query Frame = 1 
Query:    1 QSSSFSV---RPLKHNAPRTNTIKAMAEGKTETPTKEAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 393
            QSS+ S    +P    A  + + K  AE +T     EAP GF+PPELDP+TPSPIF GSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG
Sbjct:   28 QSSTLSFLTSKPHLKAASSSRSFKVSAEAETSV---EAPAGFSPPELDPSTPSPIFAGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 158

HSP 2 Score: 112.849 bits (281), Expect = 1.592e-24
Identity = 52/62 (83.87%), Postives = 55/62 (88.71%), Query Frame = 2 
Query:  356 RLSTSFTECFPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYDL 541
            ++   F   FP GEVQYLHPKDGVYPEKVNPGREGVGQN+RSIGKNVSPIEVKFTGKQVYDL
Sbjct:  146 KIKYQFYRVFPNGEVQYLHPKDGVYPEKVNPGREGVGQNFRSIGKNVSPIEVKFTGKQVYDL 207
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD1_ARATH (Photosystem I reaction center subunit II-1, chloroplastic OS=Arabidopsis thaliana GN=psaD1 PE=1 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 2.630e-51
Identity = 99/118 (83.90%), Postives = 101/118 (85.59%), Query Frame = 1 
Query:   40 APRTNTIKAMAEGKTETPTKEAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 393
            A R     + A       TKEAPVGFTPP+LDPNTPSPIF GSTGGLLRKAQVEEFYVITW SPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKI YQFYRVFPNG
Sbjct:   42 AIRAEKTDSSAAAAAAPATKEAPVGFTPPQLDPNTPSPIFAGSTGGLLRKAQVEEFYVITWNSPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKITYQFYRVFPNG 159

HSP 2 Score: 105.145 bits (261), Expect = 3.320e-22
Identity = 49/62 (79.03%), Postives = 52/62 (83.87%), Query Frame = 2 
Query:  356 RLSTSFTECFPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYDL 541
            +++  F   FP GEVQYLHPKDGVYPEK NPGREGVG N RSIGKNVSPIEVKFTGKQ YDL
Sbjct:  147 KITYQFYRVFPNGEVQYLHPKDGVYPEKANPGREGVGLNMRSIGKNVSPIEVKFTGKQSYDL 208
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD2_ARATH (Photosystem I reaction center subunit II-2, chloroplastic OS=Arabidopsis thaliana GN=psaD2 PE=1 SV=1)

HSP 1 Score: 201.445 bits (511), Expect = 3.435e-51
Identity = 102/129 (79.07%), Postives = 106/129 (82.17%), Query Frame = 1 
Query:   10 SFSVRPLKHNAPRTNTIKAMAEGKTETPT-KEAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 393
            S S RP   +  +T       E  +  P  KEAPVGFTPP+LDPNTPSPIF GSTGGLLRKAQVEEFYVITW SPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKI YQFYRVFPNG
Sbjct:   27 SSSHRPKSLSFTKTAIRAEKTESSSAAPAVKEAPVGFTPPQLDPNTPSPIFAGSTGGLLRKAQVEEFYVITWNSPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKITYQFYRVFPNG 155

HSP 2 Score: 105.145 bits (261), Expect = 3.320e-22
Identity = 49/62 (79.03%), Postives = 52/62 (83.87%), Query Frame = 2 
Query:  356 RLSTSFTECFPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYDL 541
            +++  F   FP GEVQYLHPKDGVYPEK NPGREGVG N RSIGKNVSPIEVKFTGKQ YDL
Sbjct:  143 KITYQFYRVFPNGEVQYLHPKDGVYPEKANPGREGVGLNMRSIGKNVSPIEVKFTGKQSYDL 204
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_HORVU (Photosystem I reaction center subunit II, chloroplastic OS=Hordeum vulgare GN=psaD PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.084e-48
Identity = 90/101 (89.11%), Postives = 94/101 (93.07%), Query Frame = 1 
Query:   91 PTKEAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIKYQFYRVFPNG 393
            P + AP GF PP+LDP+TPSPIFGGSTGGLLRKAQVEEFYVITW SPKEQ+FEMPTGGAAIMREGPNLLKLARKEQCLALG RLRSKYKI YQFYRVFPNG
Sbjct:   56 PAEPAPAGFVPPQLDPSTPSPIFGGSTGGLLRKAQVEEFYVITWTSPKEQVFEMPTGGAAIMREGPNLLKLARKEQCLALGNRLRSKYKIAYQFYRVFPNG 156

HSP 2 Score: 102.449 bits (254), Expect = 2.152e-21
Identity = 46/62 (74.19%), Postives = 53/62 (85.48%), Query Frame = 2 
Query:  356 RLSTSFTECFPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYDL 541
            +++  F   FP GEVQYLHPKDGVYPEKVN GR+GVGQN+RSIGKNVSPIEVKFTGK  +D+
Sbjct:  144 KIAYQFYRVFPNGEVQYLHPKDGVYPEKVNAGRQGVGQNFRSIGKNVSPIEVKFTGKNSFDI 205
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_CYAPA (Photosystem I reaction center subunit II, cyanelle OS=Cyanophora paradoxa GN=psaD PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.308e-48
Identity = 65/98 (66.33%), Postives = 79/98 (80.61%), Query Frame = 1 
Query:  103 APVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKI-KYQFYRVFPNG 393
            AP  F+ P L+ N P+PIFGGSTGGLLRKA+VEEFY ITW    E +FE+PTGGAAIMR G NLL+LARKEQC+ALG +L+ K+KI  Y+ YRV+P+G
Sbjct:   70 APKPFSVPTLNLNAPTPIFGGSTGGLLRKAEVEEFYSITWTGKSETVFELPTGGAAIMRAGENLLRLARKEQCIALGAQLKDKFKITDYKIYRVYPSG 167

HSP 2 Score: 76.6406 bits (187), Expect = 2.308e-48
Identity = 32/52 (61.54%), Postives = 41/52 (78.85%), Query Frame = 2 
Query:  383 FPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYD 538
            +P GEVQ+LHPKDGV+PEKVNPGR  VG N R IG+N  P ++KF G++ +D
Sbjct:  164 YPSGEVQFLHPKDGVFPEKVNPGRVAVGSNKRRIGQNPDPAKLKFKGQETFD 215
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_PORPU (Photosystem I reaction center subunit II OS=Porphyra purpurea GN=psaD PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 8.593e-45
Identity = 60/92 (65.22%), Postives = 69/92 (75.00%), Query Frame = 1 
Query:  121 PPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIK-YQFYRVFPNG 393
            P  ++ N PSP FGGSTGG LR A+VEE Y ITW    E  FEMPTGG A MR+G NLL LA+KEQCLALGT+L+ K+KI  Y+ YRVFPNG
Sbjct:    2 PDTINLNMPSPTFGGSTGGWLRAAEVEEKYAITWTGKNESKFEMPTGGTATMRDGENLLYLAKKEQCLALGTQLKGKFKISDYKIYRVFPNG 93

HSP 2 Score: 78.5666 bits (192), Expect = 8.593e-45
Identity = 36/52 (69.23%), Postives = 39/52 (75.00%), Query Frame = 2 
Query:  383 FPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYD 538
            FP GEVQYLHPKDGV+PEKVN GR  +     SIGKNV+PI VKFT K  YD
Sbjct:   90 FPNGEVQYLHPKDGVFPEKVNAGRASINSVDHSIGKNVNPINVKFTNKATYD 141
[Close]
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Match: PSAD_PORYE (Photosystem I reaction center subunit II OS=Porphyra yezoensis GN=psaD PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 5.486e-44
Identity = 61/92 (66.30%), Postives = 69/92 (75.00%), Query Frame = 1 
Query:  121 PPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTRLRSKYKIK-YQFYRVFPNG 393
            P  ++ N PSP FGGSTGG LR A+VEE Y ITW    E  FEMPTGG A MR G NLL LA+KEQCLALGT+L+SK+KI  Y+ YRVFPNG
Sbjct:    2 PDTINLNMPSPTFGGSTGGWLRAAEVEEKYAITWTGKNESKFEMPTGGTATMRNGENLLYLAKKEQCLALGTQLKSKFKISDYKIYRVFPNG 93

HSP 2 Score: 75.0998 bits (183), Expect = 5.486e-44
Identity = 33/52 (63.46%), Postives = 39/52 (75.00%), Query Frame = 2 
Query:  383 FPMGEVQYLHPKDGVYPEKVNPGREGVGQNYRSIGKNVSPIEVKFTGKQVYD 538
            FP GEVQYLHPKDGV+PEKVN GR  V     SIG+N++P++VKF  K  YD
Sbjct:   90 FPNGEVQYLHPKDGVFPEKVNTGRASVNSVDHSIGQNINPVDVKFMNKATYD 141
The following BLAST results are available for this feature:
BLAST of BQ623830 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 26
Match NameE-valueIdentityDescription
PSAD_NICSY1.261e-5381.40Photosystem I reaction center subunit II, chloropl... [more]
PSAD_SOLLC3.670e-5381.54Photosystem I reaction center subunit II, chloropl... [more]
PSAD_SPIOL4.794e-5386.89Photosystem I reaction center subunit II, chloropl... [more]
PSAD_CUCSA1.542e-5177.61Photosystem I reaction center subunit II, chloropl... [more]
PSAD1_ARATH2.630e-5183.90Photosystem I reaction center subunit II-1, chloro... [more]
PSAD2_ARATH3.435e-5179.07Photosystem I reaction center subunit II-2, chloro... [more]
PSAD_HORVU2.084e-4889.11Photosystem I reaction center subunit II, chloropl... [more]
PSAD_CYAPA2.308e-4866.33Photosystem I reaction center subunit II, cyanelle... [more]
PSAD_PORPU8.593e-4565.22Photosystem I reaction center subunit II OS=Porphy... [more]
PSAD_PORYE5.486e-4466.30Photosystem I reaction center subunit II OS=Porphy... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_00910 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_00910 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ623830 ID=BQ623830; Name=BQ623830; organism=Citrus sinensis; type=EST; length=669bp
CAATCATCATCATTTTCAGTAAGGCCACTAAAACATAATGCCCCACGTAC
CAACACCATCAAGGCCATGGCTGAGGGAAAAACAGAGACACCCACAAAAG
AGGCTCCGGTGGGCTTCACCCCACCAGAACTGGACCCCAACACCCCATCT
CCGATCTTCGGAGGCAGCACCGGCGGGCTGCTGCGGAAGGCTCAGGTGGA
AGAGTTTTATGTGATCACATGGGAGTCTCCTAAGGAGCAAATATTTGAAA
TGCCCACTGGGGGTGCGGCCATTATGAGAGAAGGTCCGAATTTGCTGAAG
CTGGCTAGGAAAGAGCAGTGCTTGGCTCTGGGGACAAGGCTGAGGTCCAA
GTACAAGATTAAGTACCAGTTTTACCGAGTGTTTCCCAATGGGAGAGGTT
CAGTATTTGCATCCCAAGGATGGTGTTTATCCTGAGAAAGTGAACCCTGG
AAGGGAAGGAGTTGGGCAGAATTACAGGTCTATTGGTAAGAATGTGAGTC
CTATTGAGGTCAAGTTTACAGGGAAGCAAGTGTATGATCTGTAAATTTAA
ATTGTAAGCTGCTAAGCTGACGGGCCTCGGATGATCTGTTGATTAATTGT
TGTGATCATTTACTTGTTTCTATTCGTGTTTTCTTTTCCTTAAAATATTG
TATGAAAGCAAGACTTGAT
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