BQ624227

Overview
NameBQ624227
Unique NameBQ624227
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length578
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP13_ARATH (Aquaporin TIP1-3 OS=Arabidopsis thaliana GN=TIP1-3 PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 4.864e-57
Identity = 55/66 (83.33%), Postives = 60/66 (90.91%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            V+ WNAVVFEIVMTFGLVYTVYATAVDPKKG++G IAP+AIG IVGANIL GGAFDGASMNP+  F
Sbjct:  139 VTPWNAVVFEIVMTFGLVYTVYATAVDPKKGDIGIIAPLAIGLIVGANILVGGAFDGASMNPAVSF 204

HSP 2 Score: 105.531 bits (262), Expect = 4.864e-57
Identity = 45/53 (84.91%), Postives = 48/53 (90.57%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAVSFGPAVVSW WTNHWVYW+GPFIGAAIAAIVYD IFI  N H+PLP+NDF
Sbjct:  200 PAVSFGPAVVSWIWTNHWVYWVGPFIGAAIAAIVYDTIFIGSNGHEPLPSNDF 252

HSP 3 Score: 43.5134 bits (101), Expect = 4.864e-57
Identity = 20/26 (76.92%), Postives = 24/26 (92.31%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALSSG 80
            G+VVACLLLK STGG+ET+AF+LS G
Sbjct:  113 GAVVACLLLKVSTGGMETAAFSLSYG 138
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP12_ORYSJ (Probable aquaporin TIP1-2 OS=Oryza sativa subsp. japonica GN=TIP1-2 PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 1.160e-51
Identity = 59/66 (89.39%), Postives = 62/66 (93.94%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            V +WNAVVFEIVMTFGLVYTVYATAVDPKKG+LG IAPIAIGFIVGANILAGGAFDGASMNP+  F
Sbjct:  139 VGAWNAVVFEIVMTFGLVYTVYATAVDPKKGDLGVIAPIAIGFIVGANILAGGAFDGASMNPAVSF 204

HSP 2 Score: 85.5001 bits (210), Expect = 1.160e-51
Identity = 36/53 (67.92%), Postives = 41/53 (77.36%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAVSFGPAVV+  W NHWVYWLGPF+GAAIAA++YD IFI    H  LP  D+
Sbjct:  200 PAVSFGPAVVTGVWDNHWVYWLGPFVGAAIAALIYDIIFIGQRPHDQLPTADY 252

HSP 3 Score: 39.2762 bits (90), Expect = 1.160e-51
Identity = 18/26 (69.23%), Postives = 21/26 (80.77%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALSSG 80
            GSVVACLLLK +TGG    AF+LS+G
Sbjct:  113 GSVVACLLLKIATGGAAVGAFSLSAG 138
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP12_ARATH (Aquaporin TIP1-2 OS=Arabidopsis thaliana GN=TIP1-2 PE=1 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 2.556e-51
Identity = 57/66 (86.36%), Postives = 60/66 (90.91%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            V S NA+VFEIVMTFGLVYTVYATAVDPK G+LGTIAPIAIGFIVGANILAGGAF GASMNP+  F
Sbjct:  140 VGSLNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAF 205

HSP 2 Score: 95.9005 bits (237), Expect = 2.556e-51
Identity = 37/53 (69.81%), Postives = 44/53 (83.02%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAV+FGPAVVSWTWTNHWVYW GP IG  +A I+YD +FID+NAH+ LP  D+
Sbjct:  201 PAVAFGPAVVSWTWTNHWVYWAGPLIGGGLAGIIYDFVFIDENAHEQLPTTDY 253

HSP 3 Score: 34.6538 bits (78), Expect = 2.556e-51
Identity = 16/26 (61.54%), Postives = 18/26 (69.23%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALSSG 80
            GSV AC LL F+TGG    AF LS+G
Sbjct:  114 GSVAACFLLSFATGGEPIPAFGLSAG 139
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP11_ARATH (Aquaporin TIP1-1 OS=Arabidopsis thaliana GN=TIP1-1 PE=1 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 5.275e-48
Identity = 54/62 (87.10%), Postives = 57/62 (91.94%), Query Frame = 1 
Query:   94 NAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            NA VFEIVMTFGLVYTVYATA+DPK G+LGTIAPIAIGFIVGANILAGGAF GASMNP+  F
Sbjct:  143 NAFVFEIVMTFGLVYTVYATAIDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAF 204

HSP 2 Score: 80.8777 bits (198), Expect = 5.275e-48
Identity = 32/53 (60.38%), Postives = 40/53 (75.47%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAV+FGPAVVSWTWTNHWVYW GP +G  IA ++Y+  FI +  H+ LP  D+
Sbjct:  200 PAVAFGPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVFFI-NTTHEQLPTTDY 251

HSP 3 Score: 41.9726 bits (97), Expect = 5.275e-48
Identity = 19/26 (73.08%), Postives = 22/26 (84.62%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALSSG 80
            GSVVACL+LKF+TGGL   AF LS+G
Sbjct:  113 GSVVACLILKFATGGLAVPAFGLSAG 138
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP12_MAIZE (Aquaporin TIP1-2 OS=Zea mays GN=TIP1-2 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.510e-47
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            V + NAVV E+VMTFGLVYTVYATAVDPKKG+LG IAPIAIGFIVGANILAGGAFDGASMNP+  F
Sbjct:  139 VGAMNAVVLEMVMTFGLVYTVYATAVDPKKGDLGVIAPIAIGFIVGANILAGGAFDGASMNPAVSF 204

HSP 2 Score: 80.1073 bits (196), Expect = 1.510e-47
Identity = 35/49 (71.43%), Postives = 38/49 (77.55%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLP 412
            PAVSFGPAVV+  W NHWVYW+GP  GAAIAA+VYD IFI    HQ LP
Sbjct:  200 PAVSFGPAVVTGVWENHWVYWVGPLAGAAIAALVYDIIFIGQRPHQQLP 248

HSP 3 Score: 38.891 bits (89), Expect = 1.510e-47
Identity = 18/26 (69.23%), Postives = 21/26 (80.77%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALSSG 80
            GSVVACLLLK +TGG    AF+LS+G
Sbjct:  113 GSVVACLLLKIATGGAALGAFSLSAG 138
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP11_ORYSJ (Probable aquaporin TIP1-1 OS=Oryza sativa subsp. japonica GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 7.803e-46
Identity = 57/66 (86.36%), Postives = 60/66 (90.91%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            VS W A+V EIVMTFGLVYTVYATAVDPKKG+LGTIAPIAIGFIVGANIL GGAFDGASMNP+  F
Sbjct:  138 VSVWEALVLEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGFIVGANILVGGAFDGASMNPAVSF 203

HSP 2 Score: 73.559 bits (179), Expect = 7.803e-46
Identity = 28/53 (52.83%), Postives = 40/53 (75.47%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAVSFGPA+VSW+W + WVYW+GP IG  +A ++Y+ +FI  + H+ LP  D+
Sbjct:  199 PAVSFGPALVSWSWESQWVYWVGPLIGGGLAGVIYEVLFI-SHTHEQLPTTDY 250

HSP 3 Score: 36.5798 bits (83), Expect = 7.803e-46
Identity = 16/24 (66.67%), Postives = 18/24 (75.00%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALS 74
            GS VAC LL+FSTGGL T  F L+
Sbjct:  113 GSTVACFLLRFSTGGLATGTFGLT 136
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP11_MAIZE (Aquaporin TIP1-1 OS=Zea mays GN=TIP1-1 PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.406e-44
Identity = 57/66 (86.36%), Postives = 60/66 (90.91%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            VS W A+V EIVMTFGLVYTVYATAVDPKKG+LGTIAPIAIGFIVGANIL GGAFDGASMNP+  F
Sbjct:  138 VSVWEALVLEIVMTFGLVYTVYATAVDPKKGSLGTIAPIAIGFIVGANILVGGAFDGASMNPAVSF 203

HSP 2 Score: 71.633 bits (174), Expect = 1.406e-44
Identity = 28/53 (52.83%), Postives = 39/53 (73.58%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAVSFGPA+VSW W   WVYW+GP IG  +A ++Y+ +FI  + H+ LP+ D+
Sbjct:  199 PAVSFGPALVSWEWGYQWVYWVGPLIGGGLAGVIYELLFI-SHTHEQLPSTDY 250

HSP 3 Score: 34.2686 bits (77), Expect = 1.406e-44
Identity = 15/24 (62.50%), Postives = 17/24 (70.83%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALS 74
            GS VAC LL+FSTGG  T  F L+
Sbjct:  113 GSTVACFLLRFSTGGQATGTFGLT 136
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP1_MEDTR (Probable aquaporin TIP-type OS=Medicago truncatula GN=AQP1 PE=1 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.564e-40
Identity = 52/61 (85.25%), Postives = 55/61 (90.16%), Query Frame = 1 
Query:   97 AVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            A+V EIVMTFGLVYTVYATAVDPKKGN+G IAPIAIGFIVGANIL GGAF GASMNP+  F
Sbjct:  143 ALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAPIAIGFIVGANILVGGAFTGASMNPAVSF 203

HSP 2 Score: 80.8777 bits (198), Expect = 3.564e-40
Identity = 33/53 (62.26%), Postives = 42/53 (79.25%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAVSFGPAVVSW+W+NHWVYW GP IG  IA +VY+ +FI ++ H+ LP  D+
Sbjct:  199 PAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEVLFI-NSTHEQLPTTDY 250
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP1_MEDSA (Probable aquaporin TIP-type OS=Medicago sativa GN=MCP1 PE=2 SV=2)

HSP 1 Score: 105.145 bits (261), Expect = 3.564e-40
Identity = 52/61 (85.25%), Postives = 55/61 (90.16%), Query Frame = 1 
Query:   97 AVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            A+V EIVMTFGLVYTVYATAVDPKKGN+G IAPIAIGFIVGANIL GGAF GASMNP+  F
Sbjct:  142 ALVLEIVMTFGLVYTVYATAVDPKKGNIGIIAPIAIGFIVGANILVGGAFTGASMNPAVSF 202

HSP 2 Score: 80.8777 bits (198), Expect = 3.564e-40
Identity = 33/53 (62.26%), Postives = 42/53 (79.25%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDDNAHQPLPANDF 424
            PAVSFGPAVVSW+W+NHWVYW GP IG  IA +VY+ +FI ++ H+ LP  D+
Sbjct:  198 PAVSFGPAVVSWSWSNHWVYWAGPLIGGGIAGLVYEVLFI-NSTHEQLPTTDY 249
[Close]
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Match: TIP31_MAIZE (Aquaporin TIP3-1 OS=Zea mays GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.126e-39
Identity = 42/66 (63.64%), Postives = 56/66 (84.85%), Query Frame = 1 
Query:   82 VSSWNAVVFEIVMTFGLVYTVYATAVDPKKGNLGTIAPIAIGFIVGANILAGGAFDGASMNPSSLF 279
            V  W+AV+ E VMTFGL+Y  YAT +DPK+G++GTIAP+A+GF++GAN+LAGG FDGA MNP+ +F
Sbjct:  143 VGDWHAVLLEAVMTFGLMYAYYATVIDPKRGHVGTIAPLAVGFLLGANVLAGGPFDGAGMNPARVF 208

HSP 2 Score: 72.7886 bits (177), Expect = 1.126e-39
Identity = 30/59 (50.85%), Postives = 40/59 (67.80%), Query Frame = 2 
Query:  266 PAVSFGPAVVSWTWTNHWVYWLGPFIGAAIAAIVYDHIFIDD------NAHQPLPANDF 424
            PA  FGPA+V W W +HWVYWLGPF+GA +A +VY+++ I        +AHQPL   D+
Sbjct:  204 PARVFGPALVGWRWRHHWVYWLGPFLGAGLAGLVYEYLVIPSADAAVPHAHQPLAPEDY 262

HSP 3 Score: 31.9574 bits (71), Expect = 1.126e-39
Identity = 14/26 (53.85%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:    3 GSVVACLLLKFSTGGLETSAFALSSG 80
            G+V A LLL+ +TGG+    FAL+SG
Sbjct:  117 GAVAATLLLRLATGGMRPPGFALASG 142
The following BLAST results are available for this feature:
BLAST of BQ624227 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 75
Match NameE-valueIdentityDescription
TIP13_ARATH4.864e-5783.33Aquaporin TIP1-3 OS=Arabidopsis thaliana GN=TIP1-3... [more]
TIP12_ORYSJ1.160e-5189.39Probable aquaporin TIP1-2 OS=Oryza sativa subsp. j... [more]
TIP12_ARATH2.556e-5186.36Aquaporin TIP1-2 OS=Arabidopsis thaliana GN=TIP1-2... [more]
TIP11_ARATH5.275e-4887.10Aquaporin TIP1-1 OS=Arabidopsis thaliana GN=TIP1-1... [more]
TIP12_MAIZE1.510e-4784.85Aquaporin TIP1-2 OS=Zea mays GN=TIP1-2 PE=2 SV=1[more]
TIP11_ORYSJ7.803e-4686.36Probable aquaporin TIP1-1 OS=Oryza sativa subsp. j... [more]
TIP11_MAIZE1.406e-4486.36Aquaporin TIP1-1 OS=Zea mays GN=TIP1-1 PE=2 SV=1[more]
TIP1_MEDTR3.564e-4085.25Probable aquaporin TIP-type OS=Medicago truncatula... [more]
TIP1_MEDSA3.564e-4085.25Probable aquaporin TIP-type OS=Medicago sativa GN=... [more]
TIP31_MAIZE1.126e-3963.64Aquaporin TIP3-1 OS=Zea mays GN=TIP3-1 PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_01318 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone USDA-FP_01318 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>BQ624227 ID=BQ624227; Name=BQ624227; organism=Citrus sinensis; type=EST; length=578bp
TAGGATCAGTTGTTGCTTGCTTGCTGCTCAAGTTTTCCACCGGTGGATTG
GAAACATCGGCATTCGCACTATCATCTGGCCGTCTCATCATGGAATGCAG
TTGTGTTTGAGATTGTGATGACCTTTGGCTTGGTCTACACAGTGTATGCA
ACAGCAGTGGATCCAAAGAAGGGAAATCTGGGCACTATTGCACCAATTGC
AATCGGTTTCATTGTTGGTGCCAACATTTTGGCTGGTGGCGCTTTTGATG
GTGCATCAATGAACCCCAGCAGTCTCTTTTGGTCCTGCTGTGGTTAGCTG
GACTTGGACTAACCACTGGGTCTACTGGCTTGGACCATTTATTGGTGCCG
CGATTGCGGCCATTGTTTACGACCACATCTTCATCGATGACAACGCTCAC
CAGCCACTTCCTGCCAATGATTTCTAAGAATCTAATTAATCATAAACCTT
TGTCTCTCTTATCGTTTTAGATCGTGGAGAGAATTGAAGCTGTGTTTCTT
TGTACTTTTATTGTTGGTGCTGTTGTTATCATTTATATTAGAAGGAGTGG
ATGGATTTATGTCTTGCTAAAAAAAAAA
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