CF417162 | Citrus Genome Database

Overview


Name	CF417162
Unique Name	CF417162
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	710

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig8110	contig	Csv1_Contig8110:682..1389.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: MTDH_FRAAN (Probable mannitol dehydrogenase OS=Fragaria ananassa GN=CAD PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 2.942e-60
Identity = 103/123 (83.74%), Postives = 112/123 (91.06%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            KFAKAMGVKVTVISTSP K+ EA++ LGADSFLVSRDQD+MQAA+GTMDGIIDTVSA HPL+PLIGLL S GKLV+VGAPEKPLELP F LLMGRK+V GS IGGMKETQEMIDFAA+HNI A
Sbjct:  199 KFAKAMGVKVTVISTSPKKEEEALKHLGADSFLVSRDQDQMQAAIGTMDGIIDTVSAQHPLLPLIGLLNSHGKLVMVGAPEKPLELPVFPLLMGRKMVAGSGIGGMKETQEMIDFAARHNITA 321

HSP 2 Score: 49.2914 bits (116), Expect = 2.942e-60
Identity = 22/26 (84.62%), Postives = 24/26 (92.31%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            DIEVIP DY+NTA+ERL KADVRYRF
Sbjct:  322 DIEVIPIDYLNTAMERLVKADVRYRF 347

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: MTDH_MESCR (Probable mannitol dehydrogenase OS=Mesembryanthemum crystallinum GN=ELI3 PE=2 SV=1)

HSP 1 Score: 193.741 bits (491), Expect = 8.408e-57
Identity = 92/123 (74.80%), Postives = 110/123 (89.43%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            KF KA G KVTVISTSP+KK EA+ RLGADSF+VSR+ ++MQ+AMGT+DGIIDTVSA HPL+PL+GLLKSQGK+++VG P+KPLELP F LL GRKI+ GS IGGMKETQEMIDFAAKH+I++
Sbjct:  202 KFGKAFGAKVTVISTSPNKKDEAVNRLGADSFVVSREPEQMQSAMGTLDGIIDTVSAAHPLLPLLGLLKSQGKMIMVGVPDKPLELPVFPLLQGRKILAGSCIGGMKETQEMIDFAAKHDIKS 324

HSP 2 Score: 48.1358 bits (113), Expect = 8.408e-57
Identity = 21/26 (80.77%), Postives = 23/26 (88.46%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            DIEV+P DYVNTA+ERL K DVRYRF
Sbjct:  325 DIEVVPMDYVNTAMERLLKGDVRYRF 350

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: MTDH_PETCR (Mannitol dehydrogenase (Fragment) OS=Petroselinum crispum GN=ELI3 PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 2.876e-54
Identity = 91/123 (73.98%), Postives = 105/123 (85.37%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            K AKA G  VTVISTS SKK EA+E+LGAD FLVS D D+MQAA GT+ GIIDTVSA+HP++PL+GLLK  GKLV+VGAPEKPLELP F LLMGRK++ GS IGG+KETQEM+DFAA+HNI A
Sbjct:  178 KMAKAFGAHVTVISTSESKKQEALEKLGADEFLVSSDSDQMQAATGTLHGIIDTVSALHPVVPLLGLLKVNGKLVMVGAPEKPLELPVFPLLMGRKVLAGSNIGGLKETQEMLDFAAQHNITA 300

HSP 2 Score: 49.2914 bits (116), Expect = 2.876e-54
Identity = 21/26 (80.77%), Postives = 24/26 (92.31%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            D+EVIP DYVNTA+ERL K+DVRYRF
Sbjct:  301 DVEVIPVDYVNTAMERLVKSDVRYRF 326

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: CADH7_ARATH (Cinnamyl alcohol dehydrogenase 7 OS=Arabidopsis thaliana GN=CAD7 PE=1 SV=2)

HSP 1 Score: 184.111 bits (466), Expect = 4.884e-54
Identity = 90/123 (73.17%), Postives = 106/123 (86.18%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            KFAKAMG KVTVISTS  K+ EA+ RLGAD+FLVSRD  +M+ AMGTMDGIIDTVSA HPL+PL+GLLK++GKLV+VGAP +PLELP F L+ GRK+V GSM+GG+KETQEM+D A KHNI A
Sbjct:  197 KFAKAMGTKVTVISTSERKRDEAVTRLGADAFLVSRDPKQMKDAMGTMDGIIDTVSATHPLLPLLGLLKNKGKLVMVGAPAEPLELPVFPLIFGRKMVVGSMVGGIKETQEMVDLAGKHNITA 319

HSP 2 Score: 48.521 bits (114), Expect = 4.884e-54
Identity = 22/26 (84.62%), Postives = 25/26 (96.15%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            DIE+I ADYVNTA+ERLAKADV+YRF
Sbjct:  320 DIELISADYVNTAMERLAKADVKYRF 345

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: CADH8_ARATH (Cinnamyl alcohol dehydrogenase 8 OS=Arabidopsis thaliana GN=CAD8 PE=1 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 9.471e-53
Identity = 91/123 (73.98%), Postives = 103/123 (83.74%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            KFAKAMG KVTVISTS  K+ EAI RLGAD+FLVSRD  +++ AMGTMDGIIDTVSA H L+PL+GLLK +GKLV+VGAPEKPLELP   L+  RK+V GSMIGG+KETQEMID A KHNI A
Sbjct:  197 KFAKAMGTKVTVISTSEKKRDEAINRLGADAFLVSRDPKQIKDAMGTMDGIIDTVSATHSLLPLLGLLKHKGKLVMVGAPEKPLELPVMPLIFERKMVMGSMIGGIKETQEMIDMAGKHNITA 319

HSP 2 Score: 47.7506 bits (112), Expect = 9.471e-53
Identity = 22/26 (84.62%), Postives = 24/26 (92.31%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            DIE+I ADYVNTA+ERL KADVRYRF
Sbjct:  320 DIELISADYVNTAMERLEKADVRYRF 345

HSP 3 Score: 22.3274 bits (46), Expect = 9.471e-53
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 3

Query:  498 VIDVANTMKSTP 533
            VIDVANT+K  P
Sbjct:  346 VIDVANTLKPNP 357

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: MTDH3_STYHU (Probable mannitol dehydrogenase 3 OS=Stylosanthes humilis GN=CAD3 PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 6.269e-51
Identity = 87/123 (70.73%), Postives = 107/123 (86.99%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSP-SKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIR 414
            KFAK  G+K+TVISTSP +KK EAI+ LGADSFLVSRD D+M+A   T+DGIIDTVSA H ++PLIGLLKS GKLVL+GA EKPLELP F L++GRK+VGG+++GG+KETQEMIDF+ KHN++
Sbjct:  202 KFAKTHGLKITVISTSPPTKKEEAIKNLGADSFLVSRDPDQMEAPKETLDGIIDTVSADHSIVPLIGLLKSHGKLVLIGAIEKPLELPPFPLILGRKLVGGTLVGGLKETQEMIDFSPKHNVK 324

HSP 2 Score: 45.4394 bits (106), Expect = 6.269e-51
Identity = 19/26 (73.08%), Postives = 24/26 (92.31%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            +IEV+P DYVN A++RLAKADV+YRF
Sbjct:  326 EIEVVPMDYVNIAMQRLAKADVKYRF 351

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: MTDH_MEDSA (Probable mannitol dehydrogenase OS=Medicago sativa GN=CAD1 PE=1 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 2.562e-49
Identity = 88/123 (71.54%), Postives = 104/123 (84.55%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            KF KA G+KVTVISTSP+K++EAI++LGADSFLVS+D ++M+AAMGTMD IIDT+SA H LMPL+GLLK  GKLV VG P KPLEL  F L+ GRK++GGS IGGMKETQEM+DF  KHNI A
Sbjct:  200 KFGKAFGLKVTVISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLNGKLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITA 322

HSP 2 Score: 37.7354 bits (86), Expect = 2.562e-49
Identity = 17/26 (65.38%), Postives = 21/26 (80.77%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            DIE+I    +NTA+ERL KADV+YRF
Sbjct:  323 DIELIKMHEINTAMERLHKADVKYRF 348

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: GEDH1_OCIBA (Geraniol dehydrogenase 1 OS=Ocimum basilicum GN=GEDH1 PE=1 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 5.673e-49
Identity = 83/123 (67.48%), Postives = 101/123 (82.11%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            KFAKA G KVTVISTS SKK EA++ LG D F+VS D  +MQAA+GT+DGIIDTVSA HP++PL+ LLK  GKL++VG P+KPL+LP F L+ GR+ + GS IGG+KETQEMIDFAAK+NI A
Sbjct:  201 KFAKAFGTKVTVISTSLSKKEEAMQHLGVDEFVVSTDPQQMQAAVGTLDGIIDTVSAPHPIVPLLSLLKPHGKLIVVGLPDKPLQLPVFPLIQGRRTIAGSGIGGLKETQEMIDFAAKNNIVA 323

HSP 2 Score: 45.8246 bits (107), Expect = 5.673e-49
Identity = 18/26 (69.23%), Postives = 24/26 (92.31%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            D+EVIP DY+NTA++RL K+DV+YRF
Sbjct:  324 DVEVIPIDYINTAMDRLLKSDVKYRF 349

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: MTDH_APIGR (Mannitol dehydrogenase OS=Apium graveolens GN=MTD PE=1 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 9.635e-49
Identity = 81/123 (65.85%), Postives = 99/123 (80.49%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            K AKA G +VTVI  S SK+ EA+E+LGADSFL++ DQ++M+ A  ++DGIIDTV   HPL PL  LLK  GKLV+VGAPEKP ELP FSLL GRK++GG++ GG+KETQEM+DFAAKHNI A
Sbjct:  201 KMAKAFGAQVTVIDISESKRKEALEKLGADSFLLNSDQEQMKGARSSLDGIIDTVPVNHPLAPLFDLLKPNGKLVMVGAPEKPFELPVFSLLKGRKLLGGTINGGIKETQEMLDFAAKHNITA 323

HSP 2 Score: 48.9062 bits (115), Expect = 9.635e-49
Identity = 21/26 (80.77%), Postives = 24/26 (92.31%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            D+EVIP DYVNTA+ERL K+DVRYRF
Sbjct:  324 DVEVIPMDYVNTAMERLVKSDVRYRF 349

[Close]

BLAST of CF417162 vs. ExPASy Swiss-Prot
Match: CADH6_ARATH (Probable cinnamyl alcohol dehydrogenase 6 OS=Arabidopsis thaliana GN=CAD6 PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 9.636e-49
Identity = 83/123 (67.48%), Postives = 103/123 (83.74%), Query Frame = 1

Query:   49 KFAKAMGVKVTVISTSPSKKSEAIERLGADSFLVSRDQDEMQAAMGTMDGIIDTVSAVHPLMPLIGLLKSQGKLVLVGAPEKPLELPAFSLLMGRKIVGGSMIGGMKETQEMIDFAAKHNIRA 417
            +FAKA G KVTV+S++  K  +A++ LGAD FLVS D+D+M+AAMGTMDGIIDTVSA H + PLIGLLKS GKLVL+GA EKP ++ AFSL++GRK + GS IGGM+ETQEMIDFAA+H I+A
Sbjct:  202 RFAKAFGTKVTVVSSTTGKSKDALDTLGADGFLVSTDEDQMKAAMGTMDGIIDTVSASHSISPLIGLLKSNGKLVLLGATEKPFDISAFSLILGRKSIAGSGIGGMQETQEMIDFAAEHGIKA 324

HSP 2 Score: 44.2838 bits (103), Expect = 9.636e-49
Identity = 19/26 (73.08%), Postives = 23/26 (88.46%), Query Frame = 2

Query:  419 DIEVIPADYVNTALERLAKADVRYRF 496
            +IE+I  DYVNTA++RLAK DVRYRF
Sbjct:  325 EIEIISMDYVNTAMDRLAKGDVRYRF 350

The following BLAST results are available for this feature:

BLAST of CF417162 vs. ExPASy Swiss-Prot

BLAST of CF417162 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 51


Match Name	E-value	Identity	Description
MTDH_FRAAN	2.942e-60	83.74	Probable mannitol dehydrogenase OS=Fragaria ananas...	[more]
MTDH_MESCR	8.408e-57	74.80	Probable mannitol dehydrogenase OS=Mesembryanthemu...	[more]
MTDH_PETCR	2.876e-54	73.98	Mannitol dehydrogenase (Fragment) OS=Petroselinum ...	[more]
CADH7_ARATH	4.884e-54	73.17	Cinnamyl alcohol dehydrogenase 7 OS=Arabidopsis th...	[more]
CADH8_ARATH	9.471e-53	73.98	Cinnamyl alcohol dehydrogenase 8 OS=Arabidopsis th...	[more]
MTDH3_STYHU	6.269e-51	70.73	Probable mannitol dehydrogenase 3 OS=Stylosanthes ...	[more]
MTDH_MEDSA	2.562e-49	71.54	Probable mannitol dehydrogenase OS=Medicago sativa...	[more]
GEDH1_OCIBA	5.673e-49	67.48	Geraniol dehydrogenase 1 OS=Ocimum basilicum GN=GE...	[more]
MTDH_APIGR	9.635e-49	65.85	Mannitol dehydrogenase OS=Apium graveolens GN=MTD ...	[more]
CADH6_ARATH	9.636e-49	67.48	Probable cinnamyl alcohol dehydrogenase 6 OS=Arabi...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_115000-092 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-002_B3 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417162 ID=CF417162; Name=CF417162; organism=Citrus sinensis; type=EST; length=710bp
ATGCATGTGGGTGTTGTCGGCTTGGGCGGTCTAGGCCATGTCGCCGTCAA
ATTCGCAAAGGCTATGGGGGTCAAGGTGACTGTGATCAGTACCTCCCCTA
GCAAGAAGAGTGAAGCCATTGAACGTCTTGGCGCTGATTCCTTTTTGGTC
AGTCGTGATCAAGATGAGATGCAGGCTGCCATGGGCACAATGGATGGTAT
TATCGATACAGTATCTGCAGTTCACCCTCTAATGCCGTTGATTGGACTGT
TGAAATCTCAAGGAAAGCTTGTACTTGTTGGTGCACCAGAGAAGCCACTG
GAGCTACCAGCTTTCTCCCTGCTCATGGGGAGGAAGATAGTTGGTGGCAG
CATGATTGGGGGGATGAAGGAGACTCAAGAGATGATTGATTTTGCGGCAA
AACACAACATCAGAGCTGGATATTGAGGTCATACCGGCCGACTATGTGAA
CACAGCCCTGGAGCGCCTTGCTAAAGCCGATGTTAGATATCGCTTTNGTC
ATCGACGTTGCAAACACAATGAAGTCCACCCCTTAAAATTTTCTTCCTTT
TGAGTTGCGCTTAAAATGAGGAATGTGCGTTTATTGTCGAGTCACAACAT
CGTCTAAGTTTTAGATTATGTTAGTCTTTTGTTGTGATTTCAAATCTGTT
TTTGCTTTTTAAGAATAATAATTTGGTGACGTATATTCCATCTGTGTGGA
CAAATTTATG