CF417768 | Citrus Genome Database

Overview


Name	CF417768
Unique Name	CF417768
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	716

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig11181	contig	Csv1_Contig11181:102..820.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

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BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10C_ARATH (20 kDa chaperonin, chloroplastic OS=Arabidopsis thaliana GN=CPN21 PE=1 SV=2)

HSP 1 Score: 324.324 bits (830), Expect = 3.945e-88
Identity = 168/233 (72.10%), Postives = 188/233 (80.69%), Query Frame = 2

Query:   20 MATAQLTASSIKVPARSLTSFDGLRPSSVKFASVG-GAPSQRSFRRLXXXXXXXXXPKYTSIKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILETDEIKDLKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            MA  QLTAS + + ARSL S DGLR SSVKF+S+  G   Q  FRRL         PKYTSIKPLGDRVLVKIK  EEKT GGI LPSTAQ+KPQ GEVVAVGEG+T+GK K+DI+V  G Q+IYSKYAGTE+EFN   HLIL+EDD+VGILET++IKDLKPLNDRVFIKVAEAEE TAGGLLLTE +KEKPSIG VIAVGPG LDEEG   PL ++ G+TV+YSKYAGNDFK
Sbjct:    1 MAATQLTASPVTMSARSLASLDGLRASSVKFSSLKPGTLRQSQFRRLVVKAASVVAPKYTSIKPLGDRVLVKIKEAEEKTLGGILLPSTAQSKPQGGEVVAVGEGRTIGKNKIDITVPTGAQIIYSKYAGTEVEFNDVKHLILKEDDIVGILETEDIKDLKPLNDRVFIKVAEAEEKTAGGLLLTETTKEKPSIGTVIAVGPGSLDEEGKITPLPVSTGSTVLYSKYAGNDFK 233

HSP 2 Score: 78.5666 bits (192), Expect = 3.770e-14
Identity = 41/93 (44.09%), Postives = 59/93 (63.44%), Query Frame = 2

Query:  200 IKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLD-ISVKPGTQVIYSKYAGTELE-FNGANHLILREDDVVGIL 472
            +KPL DRV +K+   EEKT GG+ L  T + KP  G V+AVG G    + K+  + V  G+ V+YSKYAG + +  +G+N++ LR  DV+ IL
Sbjct:  160 LKPLNDRVFIKVAEAEEKTAGGLLLTETTKEKPSIGTVIAVGPGSLDEEGKITPLPVSTGSTVLYSKYAGNDFKGKDGSNYIALRASDVMAIL 252

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10C_SPIOL (20 kDa chaperonin, chloroplastic OS=Spinacia oleracea GN=CPN21 PE=2 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 1.232e-73
Identity = 140/236 (59.32%), Postives = 175/236 (74.15%), Query Frame = 2

Query:   20 MATAQLTASSIKVPARSLTSFDGLRPSS----VKFASVGGAPSQRSFRRLXXXXXXXXXPKYTSIKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILETDEIKDLKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            MA   LT++S  +   +L SF+GLR +S    +  +    + + RSFR L          KYTS+KPLGDRVL+K K VEEKT  GIFLP+ AQ KPQ+GEVVA+G GK VG  KL ++VK G +V+YSKY GTE+E +G++HLI++EDD++GILETD++KDLKPLNDR+ IKVAE E  T+GGLLL E+SKEKPS G V+A GPG LDEEGNR PL +  GNTV+YSKYAGNDFK
Sbjct:    1 MAATHLTSTS-SLTINTLPSFEGLRSASGISKINVSVAYPSFTSRSFRGLVVRAASITTSKYTSVKPLGDRVLIKTKIVEEKTTSGIFLPTAAQKKPQSGEVVAIGSGKKVGDKKLPVAVKTGAEVVYSKYTGTEIEVDGSSHLIVKEDDIIGILETDDVKDLKPLNDRLLIKVAEVENKTSGGLLLAESSKEKPSFGTVVATGPGVLDEEGNRIPLPVCSGNTVLYSKYAGNDFK 235

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_RHOMR (10 kDa chaperonin OS=Rhodothermus marinus GN=groS PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 3.402e-23
Identity = 48/95 (50.53%), Postives = 72/95 (75.79%), Query Frame = 2

Query:  200 IKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILETDE 484
            IKPL DRV++K +  EEKT+ G+++P TA+ KPQ G V+AVG G+    TK+++SVK G +V+Y KYAGTE+  +G  +LI+RE D++GI+E ++
Sbjct:    6 IKPLSDRVVIKPEPPEEKTESGLYIPDTAKEKPQRGTVIAVGPGRVENGTKIEMSVKEGDKVLYGKYAGTEITIDGEEYLIMRETDILGIIEEEK 100

HSP 2 Score: 69.3218 bits (168), Expect = 2.287e-11
Identity = 35/72 (48.61%), Postives = 47/72 (65.28%), Query Frame = 2

Query:  494 LKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGND 709
            +KPL+DRV IK    EE T  GL + + +KEKP  G VIAVGPG + E G +  +S+  G+ V+Y KYAG +
Sbjct:    6 IKPLSDRVVIKPEPPEEKTESGLYIPDTAKEKPQRGTVIAVGPGRV-ENGTKIEMSVKEGDKVLYGKYAGTE 76

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_THEPX (10 kDa chaperonin OS=Thermoanaerobacter sp. (strain X514) GN=groS PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 5.803e-23
Identity = 50/92 (54.35%), Postives = 70/92 (76.09%), Query Frame = 2

Query:  200 IKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILE 475
            +KPLGDRV+VK+   EE T GG+ LP TA+ KPQ GEVVAVG G+ +   K+++ VK G +VI+SKYAGTE++ +G  +L+LRE D++ I+E
Sbjct:    3 LKPLGDRVVVKVIQAEEVTKGGVILPGTAKEKPQQGEVVAVGTGQYIDGKKVELEVKVGDRVIFSKYAGTEVKLDGEEYLLLRESDILAIIE 94

HSP 2 Score: 70.4774 bits (171), Expect = 1.027e-11
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 2

Query:  494 LKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            LKPL DRV +KV +AEE T GG++L   +KEKP  G V+AVG G    +G +  L +  G+ V++SKYAG + K
Sbjct:    3 LKPLGDRVVVKVIQAEEVTKGGVILPGTAKEKPQQGEVVAVGTGQY-IDGKKVELEVKVGDRVIFSKYAGTEVK 75

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_THEP3 (10 kDa chaperonin OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=groS PE=3 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 5.803e-23
Identity = 50/92 (54.35%), Postives = 70/92 (76.09%), Query Frame = 2

Query:  200 IKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILE 475
            +KPLGDRV+VK+   EE T GG+ LP TA+ KPQ GEVVAVG G+ +   K+++ VK G +VI+SKYAGTE++ +G  +L+LRE D++ I+E
Sbjct:    3 LKPLGDRVVVKVIQAEEVTKGGVILPGTAKEKPQQGEVVAVGTGQYIDGKKVELEVKVGDRVIFSKYAGTEVKLDGEEYLLLRESDILAIIE 94

HSP 2 Score: 70.4774 bits (171), Expect = 1.027e-11
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 2

Query:  494 LKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            LKPL DRV +KV +AEE T GG++L   +KEKP  G V+AVG G    +G +  L +  G+ V++SKYAG + K
Sbjct:    3 LKPLGDRVVVKVIQAEEVTKGGVILPGTAKEKPQQGEVVAVGTGQY-IDGKKVELEVKVGDRVIFSKYAGTEVK 75

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_THEBR (10 kDa chaperonin OS=Thermoanaerobacter brockii GN=groS PE=1 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 5.803e-23
Identity = 50/92 (54.35%), Postives = 70/92 (76.09%), Query Frame = 2

Query:  200 IKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILE 475
            +KPLGDRV+VK+   EE T GG+ LP TA+ KPQ GEVVAVG G+ +   K+++ VK G +VI+SKYAGTE++ +G  +L+LRE D++ I+E
Sbjct:    3 LKPLGDRVVVKVIQAEEVTKGGVILPGTAKEKPQQGEVVAVGTGEYIDGKKVELEVKVGDRVIFSKYAGTEVKLDGEEYLLLRESDILAIIE 94

HSP 2 Score: 70.4774 bits (171), Expect = 1.027e-11
Identity = 36/74 (48.65%), Postives = 49/74 (66.22%), Query Frame = 2

Query:  494 LKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            LKPL DRV +KV +AEE T GG++L   +KEKP  G V+AVG G    +G +  L +  G+ V++SKYAG + K
Sbjct:    3 LKPLGDRVVVKVIQAEEVTKGGVILPGTAKEKPQQGEVVAVGTGEY-IDGKKVELEVKVGDRVIFSKYAGTEVK 75

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_NATTJ (10 kDa chaperonin OS=Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) GN=groS PE=3 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.205e-22
Identity = 49/94 (52.13%), Postives = 72/94 (76.60%), Query Frame = 2

Query:  197 SIKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGK-TKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILE 475
            ++KPLGDR+++KI   EEKT+ GI LP  A+ KPQ GEVVAVG GKT+   +K++  VK G +V+YSK+AG E+E +G  +LI+R+DD++ ++E
Sbjct:    2 NLKPLGDRIVIKILEAEEKTESGIVLPEKAKEKPQEGEVVAVGSGKTLDDGSKVEPEVKAGDKVVYSKFAGNEVEVDGEEYLIMRQDDILAVIE 95

HSP 2 Score: 75.0998 bits (183), Expect = 4.168e-13
Identity = 35/73 (47.95%), Postives = 51/73 (69.86%), Query Frame = 2

Query:  491 DLKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGND 709
            +LKPL DR+ IK+ EAEE T  G++L E +KEKP  G V+AVG G   ++G++    +  G+ V+YSK+AGN+
Sbjct:    2 NLKPLGDRIVIKILEAEEKTESGIVLPEKAKEKPQEGEVVAVGSGKTLDDGSKVEPEVKAGDKVVYSKFAGNE 74

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_DESVV (10 kDa chaperonin OS=Desulfovibrio vulgaris subsp. vulgaris (strain DP4) GN=groS PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 6.416e-22
Identity = 50/94 (53.19%), Postives = 69/94 (73.40%), Query Frame = 2

Query:  197 SIKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKL-DISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILE 475
            ++KPL DRVLVK    EEKT GG+++P TA+ KP  GEVVA G GKT    KL  ++VK G  V+++KYAGTE++ +G  HL++REDD++ I+E
Sbjct:    2 NLKPLNDRVLVKRLESEEKTAGGLYIPDTAKEKPSRGEVVAAGPGKTADDGKLVAMTVKAGDMVLFNKYAGTEIKIDGVEHLVMREDDILAIIE 95

HSP 2 Score: 80.1073 bits (196), Expect = 1.296e-14
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 2

Query:  491 DLKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            +LKPLNDRV +K  E+EE TAGGL + + +KEKPS G V+A GPG   ++G    +++  G+ V+++KYAG + K
Sbjct:    2 NLKPLNDRVLVKRLESEEKTAGGLYIPDTAKEKPSRGEVVAAGPGKTADDGKLVAMTVKAGDMVLFNKYAGTEIK 76

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_DESVH (10 kDa chaperonin OS=Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) GN=groS PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 6.416e-22
Identity = 50/94 (53.19%), Postives = 69/94 (73.40%), Query Frame = 2

Query:  197 SIKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKL-DISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGILE 475
            ++KPL DRVLVK    EEKT GG+++P TA+ KP  GEVVA G GKT    KL  ++VK G  V+++KYAGTE++ +G  HL++REDD++ I+E
Sbjct:    2 NLKPLNDRVLVKRLESEEKTAGGLYIPDTAKEKPSRGEVVAAGPGKTADDGKLVAMTVKAGDMVLFNKYAGTEIKIDGVEHLVMREDDILAIIE 95

HSP 2 Score: 80.1073 bits (196), Expect = 1.296e-14
Identity = 36/75 (48.00%), Postives = 53/75 (70.67%), Query Frame = 2

Query:  491 DLKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            +LKPLNDRV +K  E+EE TAGGL + + +KEKPS G V+A GPG   ++G    +++  G+ V+++KYAG + K
Sbjct:    2 NLKPLNDRVLVKRLESEEKTAGGLYIPDTAKEKPSRGEVVAAGPGKTADDGKLVAMTVKAGDMVLFNKYAGTEIK 76

[Close]

BLAST of CF417768 vs. ExPASy Swiss-Prot
Match: CH10_DESHY (10 kDa chaperonin OS=Desulfitobacterium hafniense (strain Y51) GN=groS PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 6.416e-22
Identity = 49/92 (53.26%), Postives = 69/92 (75.00%), Query Frame = 2

Query:  197 SIKPLGDRVLVKIKTVEEKTDGGIFLPSTAQTKPQAGEVVAVGEGKTVGKTKLDISVKPGTQVIYSKYAGTELEFNGANHLILREDDVVGIL 472
            +IKPLGDRV++K   +EEKT  GI +P TA+ KPQ GEVVAVG GK     ++ + VK G +VIYSKYAGTE++++G  +LIL+E D++ ++
Sbjct:    2 NIKPLGDRVVIKALPMEEKTKSGIIMPDTAKEKPQEGEVVAVGPGKMEKGERIVLDVKVGDRVIYSKYAGTEVKYDGQEYLILKETDILAVI 93

HSP 2 Score: 73.1738 bits (178), Expect = 1.584e-12
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 2

Query:  491 DLKPLNDRVFIKVAEAEETTAGGLLLTEASKEKPSIGMVIAVGPGPLDEEGNRKPLSIAPGNTVMYSKYAGNDFK 715
            ++KPL DRV IK    EE T  G+++ + +KEKP  G V+AVGPG + E+G R  L +  G+ V+YSKYAG + K
Sbjct:    2 NIKPLGDRVVIKALPMEEKTKSGIIMPDTAKEKPQEGEVVAVGPGKM-EKGERIVLDVKVGDRVIYSKYAGTEVK 75

The following BLAST results are available for this feature:

BLAST of CF417768 vs. ExPASy Swiss-Prot

BLAST of CF417768 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 494


Match Name	E-value	Identity	Description
CH10C_ARATH	3.945e-88	72.10	20 kDa chaperonin, chloroplastic OS=Arabidopsis th...	[more]
CH10C_SPIOL	1.232e-73	59.32	20 kDa chaperonin, chloroplastic OS=Spinacia olera...	[more]
CH10_RHOMR	3.402e-23	50.53	10 kDa chaperonin OS=Rhodothermus marinus GN=groS ...	[more]
CH10_THEPX	5.803e-23	54.35	10 kDa chaperonin OS=Thermoanaerobacter sp. (strai...	[more]
CH10_THEP3	5.803e-23	54.35	10 kDa chaperonin OS=Thermoanaerobacter pseudethan...	[more]
CH10_THEBR	5.803e-23	54.35	10 kDa chaperonin OS=Thermoanaerobacter brockii GN...	[more]
CH10_NATTJ	2.205e-22	52.13	10 kDa chaperonin OS=Natranaerobius thermophilus (...	[more]
CH10_DESVV	6.416e-22	53.19	10 kDa chaperonin OS=Desulfovibrio vulgaris subsp....	[more]
CH10_DESVH	6.416e-22	53.19	10 kDa chaperonin OS=Desulfovibrio vulgaris (strai...	[more]
CH10_DESHY	6.416e-22	53.26	10 kDa chaperonin OS=Desulfitobacterium hafniense ...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-698 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-008_H12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417768 ID=CF417768; Name=CF417768; organism=Citrus sinensis; type=EST; length=716bp
CTCTCTCTGCATCTGCAAGATGGCGACAGCTCAGCTAACAGCGTCATCAA
TTAAGGTTCCAGCAAGGAGCTTGACTTCATTCGATGGTCTCAGACCGTCT
TCCGTCAAGTTCGCTTCCGTCGGAGGTGCTCCCAGCCAACGCTCTTTCCG
TCGCTTGGTCGTTAAAGCCGCTGCAGTCGTGGCTCCCAAGTACACTTCAA
TTAAACCTTTGGGTGATAGAGTGCTGGTGAAAATCAAGACTGTAGAGGAG
AAAACTGATGGTGGTATTTTTCTGCCATCTACAGCTCAAACAAAGCCTCA
AGCAGGTGAGGTGGTAGCTGTTGGCGAGGGGAAGACAGTTGGGAAGACTA
AACTGGACATATCTGTGAAGCCTGGGACCCAGGTCATCTATTCGAAGTAT
GCAGGGACTGAGTTGGAGTTCAATGGCGCAAATCATCTCATATTGAGGGA
GGATGACGTAGTTGGCATTCTTGAAACAGATGAAATCAAGGATCTTAAGC
CCCTTAATGACAGAGTTTTCATCAAGGTTGCTGAGGCTGAGGAGACAACT
GCTGGAGGTTTGTTGTTGACGGAGGCAAGCAAGGAGAAACCTTCCATTGG
AATGGTCATTGCTGTTGGACCCGGTCCTCTAGATGAGGAAGGTAATAGGA
AACCTCTATCCATTGCTCCAGGGAACACGGTGATGTATTCCAAGTATGCA
GGGAATGACTTCAAGG