CF417886

Overview
NameCF417886
Unique NameCF417886
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length687
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9291 contig Csv1_Contig9291:185..867. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHS2_CITSI (Chalcone synthase 2 OS=Citrus sinensis GN=CHS2 PE=2 SV=1)

HSP 1 Score: 300.827 bits (769), Expect = 1.238e-100
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 2 
Query:    2 KSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            KSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   45 KSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 85.8853 bits (211), Expect = 1.238e-100
Identity = 51/86 (59.30%), Postives = 52/86 (60.47%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGPADTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPADTHLDSLVGQALFGDGAAAVIVGA-DPDTSVERPLYQLVSTSQTILPDSDGAIDGHLREVGLTFHLLKDV 271
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHS1_SOYBN (Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 6.141e-97
Identity = 141/147 (95.92%), Postives = 146/147 (99.32%), Query Frame = 2 
Query:    5 SEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   46 SEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 83.9593 bits (206), Expect = 6.141e-97
Identity = 50/86 (58.14%), Postives = 51/86 (59.30%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP DTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPTDTHLDSLVGQALFGDGAAAVIVG--SDPLPVEKPLFQLVWTAQTILPDSEGAIDGHLREVGLTFHLLKDV 270
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHSY_BETVE (Chalcone synthase OS=Betula verrucosa GN=CHS PE=2 SV=2)

HSP 1 Score: 289.656 bits (740), Expect = 8.011e-97
Identity = 141/148 (95.27%), Postives = 144/148 (97.30%), Query Frame = 2 
Query:    2 KSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            KS+HMTELKEKFKRMCDKSMIKKRYMYL EEIL ENPNMCAYMAPSLDARQ IVVVEVPKLGKEAATKAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   45 KSDHMTELKEKFKRMCDKSMIKKRYMYLNEEILNENPNMCAYMAPSLDARQTIVVVEVPKLGKEAATKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 84.3445 bits (207), Expect = 8.011e-97
Identity = 50/86 (58.14%), Postives = 51/86 (59.30%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP DTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPTDTHLDSLVGQALFGDGAAAVIVGA-DPDTSVERPLFELISAAQTILPDSDGAIDGHLREVGLTFHLLKDV 271
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHSY_PUELO (Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 8.011e-97
Identity = 141/147 (95.92%), Postives = 145/147 (98.64%), Query Frame = 2 
Query:    5 SEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            SEHMTELKEKF+RMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTK +GLRP VKR+MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   46 SEHMTELKEKFQRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKQLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 84.3445 bits (207), Expect = 8.011e-97
Identity = 50/86 (58.14%), Postives = 52/86 (60.47%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP+DTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPSDTHLDSLVGQALFGDGAAAVIVG-SDPIPQVEKPLYELVWTAQTIAPDSEGAIDGHLREVGLTFHLLKDV 271
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHS5_SOYBN (Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 1.364e-96
Identity = 140/147 (95.24%), Postives = 146/147 (99.32%), Query Frame = 2 
Query:    5 SEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VV+EVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   46 SEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 83.9593 bits (206), Expect = 1.364e-96
Identity = 50/86 (58.14%), Postives = 51/86 (59.30%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP DTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPTDTHLDSLVGQALFGDGAAAVIVG--SDPLPVEKPLFQLVWTAQTILPDSEGAIDGHLREVGLTFHLLKDV 270
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHS2_SOYBN (Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 1.364e-96
Identity = 140/147 (95.24%), Postives = 146/147 (99.32%), Query Frame = 2 
Query:    5 SEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VV+EVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   46 SEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVMEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 83.9593 bits (206), Expect = 1.364e-96
Identity = 50/86 (58.14%), Postives = 51/86 (59.30%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP DTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPTDTHLDSLVGQALFGDGAAAVIVG--SDPLPVEKPLFQLVWTAQTILPDSEGAIDGHLREVGLTFHLLKDV 270
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHS3_SOYBN (Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 3.950e-96
Identity = 141/147 (95.92%), Postives = 146/147 (99.32%), Query Frame = 2 
Query:    5 SEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            SEHMTELKEKFKRMCDKSMIKKRYMYL EEILKENP++CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKR+MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   46 SEHMTELKEKFKRMCDKSMIKKRYMYLNEEILKENPSVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 81.2629 bits (199), Expect = 3.950e-96
Identity = 49/86 (56.98%), Postives = 50/86 (58.14%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP DTHLDSLVGQALFGDGAAAV  G                            AIDGHL EVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPTDTHLDSLVGQALFGDGAAAVIVG--SDPLPVEKPLFQLVWTAQTILPDSEGAIDGHLGEVGLTFHLLKDV 270
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHS2_CAMSI (Chalcone synthase 2 OS=Camellia sinensis GN=CHS2 PE=2 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 6.722e-96
Identity = 140/147 (95.24%), Postives = 145/147 (98.64%), Query Frame = 2 
Query:    5 SEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            SEH TELKEKF+RMCDKSMIKKRYMYLTEEILKENPN+CAYMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHL+FCTTSGVDMPGADYQLTKL+GLRPSVKR MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   46 SEHKTELKEKFQRMCDKSMIKKRYMYLTEEILKENPNVCAYMAPSLDARQDMVVVEVPKLGKEAATKAIKEWGQPKSKITHLVFCTTSGVDMPGADYQLTKLLGLRPSVKRLMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 82.0333 bits (201), Expect = 6.722e-96
Identity = 48/86 (55.81%), Postives = 51/86 (59.30%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP+D HLDSLVGQALFGDGAAA+  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPSDAHLDSLVGQALFGDGAAAIIVG-SDPIPEVEKPLFELVSAAQTILPDSDGAIDGHLREVGLTFHLLKDV 271
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHSY_PHAVU (Chalcone synthase 17 OS=Phaseolus vulgaris GN=CHS17 PE=2 SV=1)

HSP 1 Score: 286.189 bits (731), Expect = 8.770e-96
Identity = 139/147 (94.56%), Postives = 144/147 (97.96%), Query Frame = 2 
Query:    5 SEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            SEHMT+LKEKF+RMCDKSMIKKRYM+L EEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAA KAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRP VKR+MMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   46 SEHMTDLKEKFQRMCDKSMIKKRYMHLNEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAAVKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 192

HSP 2 Score: 84.3445 bits (207), Expect = 8.770e-96
Identity = 50/86 (58.14%), Postives = 52/86 (60.47%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP+DTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  187 LVVCSEITAVTFRGPSDTHLDSLVGQALFGDGAAAVIVG-SDPIPQIEKPLFELVWTAQTIAPDSDGAIDGHLREVGLTFHLLKDV 271
[Close]
BLAST of CF417886 vs. ExPASy Swiss-Prot
Match: CHSY_CALCH (Chalcone synthase OS=Callistephus chinensis GN=CHS PE=2 SV=2)

HSP 1 Score: 286.574 bits (732), Expect = 1.144e-95
Identity = 138/148 (93.24%), Postives = 145/148 (97.97%), Query Frame = 2 
Query:    2 KSEHMTELKEKFKRMCDKSMIKKRYMYLTEEILKENPNMCAYMAPSLDARQDIVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLIGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 445
            KSEHM +LKEKFKRMCDKSMI+KRYM+LTEE LKENP++C YMAPSLDARQD+VVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKL+GLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE
Sbjct:   48 KSEHMVDLKEKFKRMCDKSMIRKRYMHLTEEYLKENPSLCEYMAPSLDARQDVVVVEVPKLGKEAATKAIKEWGQPKSKITHLIFCTTSGVDMPGADYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSE 195

HSP 2 Score: 83.5741 bits (205), Expect = 1.144e-95
Identity = 50/86 (58.14%), Postives = 51/86 (59.30%), Query Frame = 3 
Query:  429 LLSVRRITAVTFRGPADTHLDSLVGQALFGDGAAAVDRGCXXXXXXXXXXXXXXXXXXXXXXXXXXXAIDGHLREVGLTFHLLKDV 686
            L+    ITAVTFRGP DTHLDSLVGQALFGDGAAAV  G                            AIDGHLREVGLTFHLLKDV
Sbjct:  190 LVVCSEITAVTFRGPNDTHLDSLVGQALFGDGAAAVIVGA-DPDLTTERPLFEMISAAQTILPDSEGAIDGHLREVGLTFHLLKDV 274
The following BLAST results are available for this feature:
BLAST of CF417886 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 145
Match NameE-valueIdentityDescription
CHS2_CITSI1.238e-100100.00Chalcone synthase 2 OS=Citrus sinensis GN=CHS2 PE=... [more]
CHS1_SOYBN6.141e-9795.92Chalcone synthase 1 OS=Glycine max GN=CHS1 PE=3 SV... [more]
CHSY_BETVE8.011e-9795.27Chalcone synthase OS=Betula verrucosa GN=CHS PE=2 ... [more]
CHSY_PUELO8.011e-9795.92Chalcone synthase OS=Pueraria lobata GN=CHS PE=2 S... [more]
CHS5_SOYBN1.364e-9695.24Chalcone synthase 5 OS=Glycine max GN=CHS5 PE=3 SV... [more]
CHS2_SOYBN1.364e-9695.24Chalcone synthase 2 OS=Glycine max GN=CHS2 PE=3 SV... [more]
CHS3_SOYBN3.950e-9695.92Chalcone synthase 3 OS=Glycine max GN=CHS3 PE=3 SV... [more]
CHS2_CAMSI6.722e-9695.24Chalcone synthase 2 OS=Camellia sinensis GN=CHS2 P... [more]
CHSY_PHAVU8.770e-9694.56Chalcone synthase 17 OS=Phaseolus vulgaris GN=CHS1... [more]
CHSY_CALCH1.144e-9593.24Chalcone synthase OS=Callistephus chinensis GN=CHS... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_115000-816 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-011_C05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF417886 ID=CF417886; Name=CF417886; organism=Citrus sinensis; type=EST; length=687bp
AAAGAGCGAGCATATGACGGAGCTTAAAGAGAAGTTCAAGCGCATGTGTG
ACAAGTCGATGATTAAGAAGCGTTACATGTACTTAACGGAAGAGATTTTG
AAGGAAAACCCCAACATGTGCGCTTACATGGCTCCATCACTCGACGCACG
TCAAGACATTGTGGTGGTCGAAGTGCCGAAGCTCGGGAAAGAAGCTGCTA
CAAAGGCCATCAAAGAATGGGGCCAGCCCAAGTCTAAGATCACCCACCTC
ATCTTCTGCACCACCTCCGGCGTCGACATGCCCGGCGCCGACTACCAGCT
CACCAAACTGATCGGCCTGCGCCCCTCCGTCAAGCGCTTCATGATGTACC
AACAAGGATGTTTCGCCGGCGGCACTGTTCTTCGCCTCGCTAAAGACTTG
GCTGAGAACAACAAGGGCGCTCGCGTTCTTGTTGTCTGTTCGGAGGATCA
CGGCAGTCACTTTCCGCGGCCCTGCCGATACTCATCTTGATTCTCTTGTG
GGCCAGGCTTTGTTCGGTGATGGTGCTGCTGCTGTGGATCGTGGGTGCCG
ATCCTGACACGTCGGTCGAGCGTCCGTTGTATCAGCTCGTGTCCGACTTC
GCAGACGATCCTCCCTGGACTCTGACGGTGCAATTGACGGACACTTGCGC
GAAGTCGGTCTCACTTTCCATTTGCTTAAAGACGTCC
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