CF503965 | Citrus Genome Database

Overview


Name	CF503965
Unique Name	CF503965
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	639

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CF503965_ssr7	microsatellite	CF503965_ssr7:7..28.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: XCP1_ARATH (Xylem cysteine proteinase 1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 6.848e-46
Identity = 58/91 (63.74%), Postives = 75/91 (82.42%), Query Frame = 1

Query:  145 EFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGLKP 417
            +FSIVGY+ E LT+TDKL+ELFESWM +H K+Y+S EEK+HR E+F++NL HID RN E+   +SYWLGLNEF+D++HEEFK +YL   KP
Sbjct:   30 DFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEI---NSYWLGLNEFADLTHEEFKGRYLGLAKP 117

HSP 2 Score: 78.1814 bits (191), Expect = 6.848e-46
Identity = 35/51 (68.63%), Postives = 41/51 (80.39%), Query Frame = 3

Query:  486 SFFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            +F  +++ DLP+SVDWRKKGAV  VK+Q  CG CWAFS VAAVEGINQI T
Sbjct:  128 NFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITT 178

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: PAPA4_CARPA (Papaya proteinase 4 OS=Carica papaya PE=1 SV=3)

HSP 1 Score: 123.25 bits (308), Expect = 1.930e-42
Identity = 59/102 (57.84%), Postives = 80/102 (78.43%), Query Frame = 1

Query:  145 EFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGL------KPDDDEF 432
            +FSIVGYS + LTST++L++LF SWMLKH K+Y++ +EKL+R EIFKDNLK+ID RN+   + + YWLGLNEFSD+S++EFK KY+  L      +P D+EF
Sbjct:   27 DFSIVGYSQDDLTSTERLIQLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNK---MINGYWLGLNEFSDLSNDEFKEKYVGSLPEDYTNQPYDEEF 125

HSP 2 Score: 70.0922 bits (170), Expect = 1.930e-42
Identity = 32/50 (64.00%), Postives = 38/50 (76.00%), Query Frame = 3

Query:  489 FFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            F  +++ DLP+SVDWR KGAVT VK+Q  C  CWAFS VA VEGIN+I T
Sbjct:  125 FVNEDIVDLPESVDWRAKGAVTPVKHQGYCESCWAFSTVATVEGINKIKT 174

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: XCP2_ARATH (Xylem cysteine proteinase 2 OS=Arabidopsis thaliana GN=XCP2 PE=1 SV=2)

HSP 1 Score: 113.235 bits (282), Expect = 3.276e-42
Identity = 57/92 (61.96%), Postives = 69/92 (75.00%), Query Frame = 1

Query:  145 EFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGLKPD 420
            ++SIVGYS E L S DKL+ELFE+W+    K+YE+ EEK  R E+FKDNLKHID  N++     SYWLGLNEF+D+SHEEFK  YL GLK D
Sbjct:   30 DYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKK---GKSYWLGLNEFADLSHEEFKKMYL-GLKTD 117

HSP 2 Score: 79.337 bits (194), Expect = 3.276e-42
Identity = 36/50 (72.00%), Postives = 41/50 (82.00%), Query Frame = 3

Query:  489 FFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            F  ++VE +P+SVDWRKKGAV  VKNQ  CG CWAFS VAAVEGIN+IVT
Sbjct:  130 FAYRDVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEGINKIVT 179

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: PAPA3_CARPA (Caricain OS=Carica papaya PE=1 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 6.024e-41
Identity = 53/90 (58.89%), Postives = 71/90 (78.89%), Query Frame = 1

Query:  142 GEFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYLTGL 411
            G+FSIVGYS + LTST++L++LF SWML H K YE+ +EKL+R EIFKDNL +ID  N++    +SYWLGLNEF+D+S++EF  KY+  L
Sbjct:   26 GDFSIVGYSQDDLTSTERLIQLFNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKK---NNSYWLGLNEFADLSNDEFNEKYVGSL 112

HSP 2 Score: 72.4034 bits (176), Expect = 6.024e-41
Identity = 32/50 (64.00%), Postives = 40/50 (80.00%), Query Frame = 3

Query:  489 FFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            F  ++  +LP++VDWRKKGAVT V++Q  CG CWAFSAVA VEGIN+I T
Sbjct:  125 FINEDTVNLPENVDWRKKGAVTPVRHQGSCGSCWAFSAVATVEGINKIRT 174

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: PAPA2_CARPA (Chymopapain OS=Carica papaya PE=1 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 1.106e-39
Identity = 53/102 (51.96%), Postives = 75/102 (73.53%), Query Frame = 1

Query:  145 EFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL-------TGLKPDDDE 429
            +F  VGYS + LTS ++L++LF+SWMLKH K YES +EK++R EIF+DNL +ID  N++    +SYWLGLN F+D+S++EFK KY+       TGL+  D+E
Sbjct:   27 DFYTVGYSQDDLTSIERLIQLFDSWMLKHNKIYESIDEKIYRFEIFRDNLMYIDETNKK---NNSYWLGLNGFADLSNDEFKKKYVGFVAEDFTGLEHFDNE 125

HSP 2 Score: 75.485 bits (184), Expect = 1.106e-39
Identity = 33/50 (66.00%), Postives = 39/50 (78.00%), Query Frame = 3

Query:  489 FFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            F  ++V + PQS+DWR KGAVT VKNQ  CG CWAFS +A VEGIN+IVT
Sbjct:  127 FTYKHVTNYPQSIDWRAKGAVTPVKNQGACGSCWAFSTIATVEGINKIVT 176

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: PAPA1_CARPA (Papain OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 3.189e-39
Identity = 54/86 (62.79%), Postives = 71/86 (82.56%), Query Frame = 1

Query:  142 GEFSIVGYSLEVLTSTDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKY 399
            G+FSIVGYS   LTST++L++LFESWMLKH K Y++ +EK++R EIFKDNLK+ID  N++    +SYWLGLN F+DMS++EFK KY
Sbjct:   26 GDFSIVGYSQNDLTSTERLIQLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKK---NNSYWLGLNVFADMSNDEFKEKY 108

HSP 2 Score: 65.0846 bits (157), Expect = 3.189e-39
Identity = 28/43 (65.12%), Postives = 34/43 (79.07%), Query Frame = 3

Query:  510 DLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            ++P+ VDWR+KGAVT VKNQ  CG CWAFSAV  +EGI +I T
Sbjct:  133 NIPEYVDWRQKGAVTPVKNQGSCGSCWAFSAVVTIEGIIKIRT 175

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: RD21A_ARATH (Cysteine proteinase RD21a OS=Arabidopsis thaliana GN=RD21A PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 7.795e-27
Identity = 31/44 (70.45%), Postives = 37/44 (84.09%), Query Frame = 3

Query:  507 EDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            ++LP+S+DWRKKGAV  VK+Q  CG CWAFS + AVEGINQIVT
Sbjct:  135 DELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVT 178

HSP 2 Score: 66.2402 bits (160), Expect = 7.795e-27
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 1

Query:  145 EFSIVGYSLEVLTSTD------KLVELFESWMLKHGK--SYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            + SI+ Y  +   ST       +++ ++E+W++KHGK  S  S  EK  R EIFKDNL+ +D  N +     SY LGL  F+D++++E+++KYL
Sbjct:   23 DMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNEK---NLSYRLGLTRFADLTNDEYRSKYL 113

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: BROM1_ANACO (Fruit bromelain OS=Ananas comosus PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.927e-26
Identity = 31/51 (60.78%), Postives = 38/51 (74.51%), Query Frame = 3

Query:  486 SFFVQNVEDLPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            SF   N+  +PQS+DWR  GAV  VKNQNPCG CW+F+A+A VEGI +I T
Sbjct:  114 SFDDVNISAVPQSIDWRDYGAVNEVKNQNPCGSCWSFAAIATVEGIYKIKT 164

HSP 2 Score: 67.3958 bits (163), Expect = 2.927e-26
Identity = 29/70 (41.43%), Postives = 49/70 (70.00%), Query Frame = 1

Query:  190 DKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKY 399
            D +++ FE WM ++G+ Y+  +EK+ R +IFK+N+KHI+  N   +  +SY LG+N+F+DM+  EF  +Y
Sbjct:   31 DPMMKRFEEWMAEYGRVYKDDDEKMRRFQIFKNNVKHIETFNSRNE--NSYTLGINQFTDMTKSEFVAQY 98

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: ACTN_ACTDE (Actinidain OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 8.388e-26
Identity = 32/73 (43.84%), Postives = 50/73 (68.49%), Query Frame = 1

Query:  184 STDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            + D++  ++ESW++K+GKSY S  E   R EIFK+ L+ ID  N +     SY +GLN+F+D++ EEF++ YL
Sbjct:   34 TNDEVKAMYESWLIKYGKSYNSLGEWERRFEIFKETLRFIDEHNAD--TNRSYKVGLNQFADLTDEEFRSTYL 104

HSP 2 Score: 68.1662 bits (165), Expect = 8.388e-26
Identity = 29/42 (69.05%), Postives = 33/42 (78.57%), Query Frame = 3

Query:  513 LPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            LP  VDWR  GAV  +K+Q  CGGCWAFSA+A VEGIN+IVT
Sbjct:  127 LPSYVDWRSAGAVVDIKSQGECGGCWAFSAIATVEGINKIVT 168

[Close]

BLAST of CF503965 vs. ExPASy Swiss-Prot
Match: ACTN_ACTCH (Actinidain OS=Actinidia chinensis PE=1 SV=4)

HSP 1 Score: 69.3218 bits (168), Expect = 8.388e-26
Identity = 32/73 (43.84%), Postives = 50/73 (68.49%), Query Frame = 1

Query:  184 STDKLVELFESWMLKHGKSYESTEEKLHRLEIFKDNLKHIDARNRELQITSSYWLGLNEFSDMSHEEFKNKYL 402
            + D++  ++ESW++K+GKSY S  E   R EIFK+ L+ ID  N +     SY +GLN+F+D++ EEF++ YL
Sbjct:   34 TNDEVKAMYESWLIKYGKSYNSLGEWERRFEIFKETLRFIDEHNAD--TNRSYKVGLNQFADLTDEEFRSTYL 104

HSP 2 Score: 68.1662 bits (165), Expect = 8.388e-26
Identity = 29/42 (69.05%), Postives = 33/42 (78.57%), Query Frame = 3

Query:  513 LPQSVDWRKKGAVTHVKNQNPCGGCWAFSAVAAVEGINQIVT 638
            LP  VDWR  GAV  +K+Q  CGGCWAFSA+A VEGIN+IVT
Sbjct:  127 LPSYVDWRSAGAVVDIKSQGECGGCWAFSAIATVEGINKIVT 168

The following BLAST results are available for this feature:

BLAST of CF503965 vs. ExPASy Swiss-Prot

BLAST of CF503965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 125


Match Name	E-value	Identity	Description
XCP1_ARATH	6.848e-46	63.74	Xylem cysteine proteinase 1 OS=Arabidopsis thalian...	[more]
PAPA4_CARPA	1.930e-42	57.84	Papaya proteinase 4 OS=Carica papaya PE=1 SV=3	[more]
XCP2_ARATH	3.276e-42	61.96	Xylem cysteine proteinase 2 OS=Arabidopsis thalian...	[more]
PAPA3_CARPA	6.024e-41	58.89	Caricain OS=Carica papaya PE=1 SV=2	[more]
PAPA2_CARPA	1.106e-39	51.96	Chymopapain OS=Carica papaya PE=1 SV=2	[more]
PAPA1_CARPA	3.189e-39	62.79	Papain OS=Carica papaya PE=1 SV=1	[more]
RD21A_ARATH	7.795e-27	70.45	Cysteine proteinase RD21a OS=Arabidopsis thaliana ...	[more]
BROM1_ANACO	2.927e-26	60.78	Fruit bromelain OS=Ananas comosus PE=1 SV=1	[more]
ACTN_ACTDE	8.388e-26	43.84	Actinidain OS=Actinidia deliciosa PE=1 SV=1	[more]
ACTN_ACTCH	8.388e-26	43.84	Actinidain OS=Actinidia chinensis PE=1 SV=4	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_119000-453 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-11_G08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF503965 ID=CF503965; Name=CF503965; organism=Citrus sinensis; type=EST; length=639bp
GTGGGGGAGAGAGAGAGAGAGAGAGAGATGAAAATGTTATCTTCCAATTG
TAAGTTGTTGCTTCGTCTTTCTTTGTGCGTGTCTCTCTTGATGATCATAT
GCATACGCTGCTCTAGCAGTAGAACTCTTCAAGGTCATGATGGTGAGTTC
TCCATTGTGGGGTATTCACTAGAGGTGTTGACATCCACGGACAAGCTGGT
TGAGCTATTCGAGTCATGGATGTTGAAACATGGGAAATCGTATGAAAGCA
CGGAAGAGAAGTTGCATAGATTGGAGATATTCAAAGACAACTTGAAGCAC
ATTGATGCGAGGAACAGAGAGCTGCAAATTACTAGTAGCTATTGGCTTGG
TCTGAATGAGTTTTCAGATATGAGCCACGAAGAGTTCAAGAACAAGTACT
TAACAGGACTCAAACCCGATGATGATGAGTTCCGCAGAAGGAGTCACCGC
GACTCCAGTAGTAGATCTTCTGCTAGCAGTAGTAATCATTTTTTGTACAA
AATGTAGAGGATTTGCCTCAATCCGTTGACTGGAGGAAGAAAGGAGCTGT
CACTCATGTCAAAAACCAAAATCCTTGTGGTGGATGTTGGGCATTTTCAG
CAGTAGCAGCAGTGGAGGGGATAAATCAGATAGTGACGG