CF504176

Overview
NameCF504176
Unique NameCF504176
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length414
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig7947 contig Csv1_Contig7947:104..517. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: MOMAS_ORYSJ (Momilactone A synthase OS=Oryza sativa subsp. japonica GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 1.230e-29
Identity = 62/107 (57.94%), Postives = 79/107 (73.83%), Query Frame = 3 
Query:   90 AKVFAQQGAKVVIADIQEQLGHSVVESIGTSNSSYVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIKHAARVM 410
            A++F + GA+VV+ADIQ++LG S+V  +G   SSYVHCDVTNE  +  A+D  VA  GKLD+MFNNAG+ G    R+ +  K DFERVL+VN+ G FLG KHAARVM
Sbjct:   34 ARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFLGTKHAARVM 140
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: TS2_MAIZE (Sex determination protein tasselseed-2 OS=Zea mays GN=TS2 PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.143e-17
Identity = 49/114 (42.98%), Postives = 74/114 (64.91%), Query Frame = 3 
Query:   84 APAKVFAQQGAKVVIADIQEQLGHSVVESIGTSNSSYVHCDVTNESHIKNAIDQTVATHG-KLDIMFNNAGIGGANKTR----IIDNEKADFERVLSVNVTGVFLGIKHAARVM 410
            A  ++FA+ GA+VVIADI +  G ++  ++G    S+V CDV+ E  ++ A+D  ++ HG +LD+  NNAG+ G  +TR    I+  + A+F+RVL VN  G  LG+KHAAR M
Sbjct:   70 AIVRLFAKHGARVVIADIDDAAGEALASALGPQ-VSFVRCDVSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLG-RQTRAARSILSFDAAEFDRVLRVNALGAALGMKHAARAM 181
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: ABA2_ARATH (Xanthoxin dehydrogenase OS=Arabidopsis thaliana GN=ABA2 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.143e-17
Identity = 49/111 (44.14%), Postives = 65/111 (58.56%), Query Frame = 3 
Query:   93 KVFAQQGAKVVIADIQEQLGHSVVESIGTSNSS----YVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIKHAARVMI 413
            ++F + GAKV I D+Q+ LG  V +S+    S     ++H DV  E  I NA+D  V   G LDI+ NNAG+ GA    I +   ++FE    VNV G FL +KHAARVMI
Sbjct:   38 RLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGAFLSMKHAARVMI 148
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: BACC_BACAM (Bacilysin biosynthesis oxidoreductase bacC OS=Bacillus amyloliquefaciens GN=bacC PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.237e-15
Identity = 43/111 (38.74%), Postives = 63/111 (56.76%), Query Frame = 3 
Query:   81 HAPAKVFAQQGAKVVIADIQEQLGHSVVESIGTSNSSYVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIKHAARVMI 413
            +A  + F  Q A VV+ADI E  G +++         +V  D+TNE   +NAI   V   G LD++ NNAGI       I + E +D+ +VL+VN+TG+FL  KHA + M+
Sbjct:   19 YAAVQAFLNQQANVVVADIDEAQGEAMIRKENNDRLHFVQTDITNEPACQNAILSAVDKFGGLDVLINNAGI--EIVAPIHEMELSDWNKVLNVNLTGMFLMSKHALKYML 127

HSP 2 Score: 24.6386 bits (52), Expect = 2.237e-15
Identity = 11/16 (68.75%), Postives = 11/16 (68.75%), Query Frame = 2 
Query:   32 LEGKVAFITGGASGIG 79
            L  K   ITGGASGIG
Sbjct:    3 LTDKTVLITGGASGIG 18
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: BACC2_BACSU (Bacilysin biosynthesis oxidoreductase bacC OS=Bacillus subtilis GN=bacC PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 2.238e-15
Identity = 42/111 (37.84%), Postives = 64/111 (57.66%), Query Frame = 3 
Query:   81 HAPAKVFAQQGAKVVIADIQEQLGHSVVESIGTSNSSYVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIKHAARVMI 413
            +A  + F  Q A VV+ADI E  G +++         +VH D+T+E   +NAI   V   G LD++ NNAGI       I + E +++ +VL+VN+TG+FL  KHA + M+
Sbjct:   19 YAAVQAFLNQQANVVVADIDEAQGEAMIRKENNDRLHFVHTDITDEPACQNAIRSAVDKFGGLDVLINNAGI--EIVAPIHEMELSNWNKVLNVNLTGMFLMSKHALKYML 127

HSP 2 Score: 24.6386 bits (52), Expect = 2.238e-15
Identity = 11/16 (68.75%), Postives = 11/16 (68.75%), Query Frame = 2 
Query:   32 LEGKVAFITGGASGIG 79
            L  K   ITGGASGIG
Sbjct:    3 LTDKTVLITGGASGIG 18
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: BACC_BACSU (Bacilysin biosynthesis oxidoreductase bacC OS=Bacillus subtilis GN=bacC PE=3 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 2.908e-15
Identity = 42/111 (37.84%), Postives = 65/111 (58.56%), Query Frame = 3 
Query:   81 HAPAKVFAQQGAKVVIADIQEQLGHSVVESIGTSNSSYVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIKHAARVMI 413
            +A  + F  Q A VV+ADI E  G ++V         +V  D+T+E+  ++A++  V T G LD++ NNAGI       I + E +D+ +VL VN+TG+FL  KHA + M+
Sbjct:   19 YAAVQAFLGQQANVVVADIDEAQGEAMVRKENNDRLHFVQTDITDEAACQHAVESAVHTFGGLDVLINNAGI--EIVAPIHEMELSDWNKVLQVNLTGMFLMSKHALKHML 127

HSP 2 Score: 24.6386 bits (52), Expect = 2.908e-15
Identity = 11/16 (68.75%), Postives = 11/16 (68.75%), Query Frame = 2 
Query:   32 LEGKVAFITGGASGIG 79
            L  K   ITGGASGIG
Sbjct:    3 LTDKTVLITGGASGIG 18
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: HSD_MYCTU (3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase OS=Mycobacterium tuberculosis GN=fabG3 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 6.376e-15
Identity = 40/106 (37.74%), Postives = 59/106 (55.66%), Query Frame = 3 
Query:   93 KVFAQQGAKVVIADIQEQLGHSVVESIGTSNSSYVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIKHAARVM 410
            +    +GAKVV  DI ++ G +V   +  + + YVH DVT  +    A+D  V   G L ++ NNAGI   N   I D    +++R+L VN+TGVFLGI+   + M
Sbjct:   25 RAMVAEGAKVVFGDILDEEGKAVAAELADA-ARYVHLDVTQPAQWTAAVDTAVTAFGGLHVLVNNAGI--LNIGTIEDYALTEWQRILDVNLTGVFLGIRAVVKPM 127

HSP 2 Score: 30.0314 bits (66), Expect = 6.376e-15
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 2 
Query:   29 RLEGKVAFITGGASGIGACTCKSLCPTGCQ 118
            RL GKVA ++GGA G+GA   +++   G +
Sbjct:    4 RLIGKVALVSGGARGMGASHVRAMVAEGAK 33
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: HSD_MYCBO (3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase OS=Mycobacterium bovis GN=fabG3 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 6.376e-15
Identity = 40/106 (37.74%), Postives = 59/106 (55.66%), Query Frame = 3 
Query:   93 KVFAQQGAKVVIADIQEQLGHSVVESIGTSNSSYVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIKHAARVM 410
            +    +GAKVV  DI ++ G +V   +  + + YVH DVT  +    A+D  V   G L ++ NNAGI   N   I D    +++R+L VN+TGVFLGI+   + M
Sbjct:   25 RAMVAEGAKVVFGDILDEEGKAVAAELADA-ARYVHLDVTQPAQWTAAVDTAVTAFGGLHVLVNNAGI--LNIGTIEDYALTEWQRILDVNLTGVFLGIRAVVKPM 127

HSP 2 Score: 30.0314 bits (66), Expect = 6.376e-15
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 2 
Query:   29 RLEGKVAFITGGASGIGACTCKSLCPTGCQ 118
            RL GKVA ++GGA G+GA   +++   G +
Sbjct:    4 RLIGKVALVSGGARGMGASHVRAMVAEGAK 33
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: CPNA_COMTE (Cyclopentanol dehydrogenase OS=Comamonas testosteroni GN=cpnA PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.820e-14
Identity = 37/101 (36.63%), Postives = 58/101 (57.43%), Query Frame = 3 
Query:   96 VFAQQGAKVVIADIQEQLGHSVVESIGTSNSS--YVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIK 392
            + A++GA V ++D+ E+LGH  V  I        ++H DVTNE+H   A+D  +A   +LD + NNAGI      +   NE  +++R+  +NV  VFLG +
Sbjct:   25 LLAREGATVYLSDMNEELGHQAVAEIRRQGGKAHFLHLDVTNENHWTGAVDTILAESDRLDALVNNAGILTLKPVQDTSNE--EWDRIFEINVRSVFLGTR 123

HSP 2 Score: 27.335 bits (59), Expect = 1.820e-14
Identity = 12/28 (42.86%), Postives = 15/28 (53.57%), Query Frame = 2 
Query:   29 RLEGKVAFITGGASGIGACTCKSLCPTG 112
            R+  KV  +TGGA G+G   C  L   G
Sbjct:    3 RVNDKVVLVTGGAMGMGLTHCTLLAREG 30
[Close]
BLAST of CF504176 vs. ExPASy Swiss-Prot
Match: CPNA_COMS9 (Cyclopentanol dehydrogenase OS=Comamonas sp. (strain NCIMB 9872) GN=cpnA PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.820e-14
Identity = 37/101 (36.63%), Postives = 58/101 (57.43%), Query Frame = 3 
Query:   96 VFAQQGAKVVIADIQEQLGHSVVESIGTSNSS--YVHCDVTNESHIKNAIDQTVATHGKLDIMFNNAGIGGANKTRIIDNEKADFERVLSVNVTGVFLGIK 392
            + A++GA V ++D+ E+LGH  V  I        ++H DVTNE+H   A+D  +A   +LD + NNAGI      +   NE  +++R+  +NV  VFLG +
Sbjct:   25 LLAREGATVYLSDMNEELGHQAVAEIRRQGGKAHFLHLDVTNENHWTGAVDTILAESDRLDALVNNAGILTLKPVQDTSNE--EWDRIFEINVRSVFLGTR 123

HSP 2 Score: 27.335 bits (59), Expect = 1.820e-14
Identity = 12/28 (42.86%), Postives = 15/28 (53.57%), Query Frame = 2 
Query:   29 RLEGKVAFITGGASGIGACTCKSLCPTG 112
            R+  KV  +TGGA G+G   C  L   G
Sbjct:    3 RVNDKVVLVTGGAMGMGLTHCTLLAREG 30
The following BLAST results are available for this feature:
BLAST of CF504176 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 16
Match NameE-valueIdentityDescription
MOMAS_ORYSJ1.230e-2957.94Momilactone A synthase OS=Oryza sativa subsp. japo... [more]
TS2_MAIZE3.143e-1742.98Sex determination protein tasselseed-2 OS=Zea mays... [more]
ABA2_ARATH3.143e-1744.14Xanthoxin dehydrogenase OS=Arabidopsis thaliana GN... [more]
BACC_BACAM2.237e-1538.74Bacilysin biosynthesis oxidoreductase bacC OS=Baci... [more]
BACC2_BACSU2.238e-1537.84Bacilysin biosynthesis oxidoreductase bacC OS=Baci... [more]
BACC_BACSU2.908e-1537.84Bacilysin biosynthesis oxidoreductase bacC OS=Baci... [more]
HSD_MYCTU6.376e-1537.743-alpha-(or 20-beta)-hydroxysteroid dehydrogenase ... [more]
HSD_MYCBO6.376e-1537.743-alpha-(or 20-beta)-hydroxysteroid dehydrogenase ... [more]
CPNA_COMTE1.820e-1436.63Cyclopentanol dehydrogenase OS=Comamonas testoster... [more]
CPNA_COMS91.820e-1436.63Cyclopentanol dehydrogenase OS=Comamonas sp. (stra... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_119000-664 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-14_G02 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504176 ID=CF504176; Name=CF504176; organism=Citrus sinensis; type=EST; length=414bp
CTCAGGGGTTCTTTCTGCAATCACCAGAAGGCTAGAGGGCAAGGTAGCCT
TTATTACTGGAGGGGCTAGTGGAATTGGTGCATGCACCTGCAAAAGTCTT
TGCCCAACAGGGTGCCAAAGTTGTCATTGCGGATATTCAGGAACAATTAG
GCCATTCAGTTGTTGAATCCATAGGCACATCAAACTCATCCTATGTCCAT
TGCGATGTCACAAACGAATCCCACATTAAAAATGCAATCGACCAAACCGT
TGCAACTCATGGCAAGCTGGACATAATGTTCAACAACGCCGGCATAGGCG
GCGCTAACAAGACCCGCATAATCGACAATGAAAAGGCCGATTTCGAGCGT
GTCCTGAGTGTAAATGTCACCGGAGTTTTCTTGGGCATCAAGCATGCTGC
TCGTGTCATGATTC
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