CF504598 | Citrus Genome Database

Overview


Name	CF504598
Unique Name	CF504598
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	383

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig10898	contig	Csv1_Contig10898:617..994.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOX_POPTR (NADP-dependent malic enzyme OS=Populus trichocarpa PE=2 SV=3)

HSP 1 Score: 120.168 bits (300), Expect = 3.464e-44
Identity = 56/62 (90.32%), Postives = 61/62 (98.39%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G+RPSACLP+T+DVGTNNEQLLKDEFYIGLRQRRATGQEY+ELL EFM+AVKQNYGEKVLIQ
Sbjct:  219 GVRPSACLPVTIDVGTNNEQLLKDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQ 280

HSP 2 Score: 61.6178 bits (148), Expect = 3.464e-44
Identity = 27/28 (96.43%), Postives = 28/28 (100.00%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF+LLAKYGTTHLVFNDDIQ
Sbjct:  281 FEDFANHNAFDLLAKYGTTHLVFNDDIQ 308

HSP 3 Score: 36.965 bits (84), Expect = 3.464e-44
Identity = 16/19 (84.21%), Postives = 18/19 (94.74%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            +LA+H FLFLGAGEAGTGI
Sbjct:  328 SLADHTFLFLGAGEAGTGI 346

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOC_FLAPR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei GN=MODA PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 8.595e-42
Identity = 54/62 (87.10%), Postives = 61/62 (98.39%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            GIRPSACLPIT+DVGTNNE++L DEFYIGLRQRRA+G+EYAEL++EFMSAVKQNYGEKVLIQ
Sbjct:  275 GIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEYAELMNEFMSAVKQNYGEKVLIQ 336

HSP 2 Score: 56.6102 bits (135), Expect = 8.595e-42
Identity = 25/28 (89.29%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF+LL KY TTHLVFNDDIQ
Sbjct:  337 FEDFANHNAFDLLEKYRTTHLVFNDDIQ 364

HSP 3 Score: 38.1206 bits (87), Expect = 8.595e-42
Identity = 16/19 (84.21%), Postives = 19/19 (100.00%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            +LA+H+FLFLGAGEAGTGI
Sbjct:  384 SLADHKFLFLGAGEAGTGI 402

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOX_VITVI (NADP-dependent malic enzyme OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.456e-41
Identity = 51/62 (82.26%), Postives = 58/62 (93.55%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G+RPSACLPIT+DVGTNNE+LL +EFYIGL+QRRATG+EY+E L EFMS VKQNYGEKVLIQ
Sbjct:  219 GVRPSACLPITIDVGTNNEKLLANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQ 280

HSP 2 Score: 60.077 bits (144), Expect = 1.456e-41
Identity = 26/28 (92.86%), Postives = 27/28 (96.43%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF+LLAKYGTTHL FNDDIQ
Sbjct:  281 FEDFANHNAFDLLAKYGTTHLAFNDDIQ 308

HSP 3 Score: 39.6614 bits (91), Expect = 1.456e-41
Identity = 17/19 (89.47%), Postives = 19/19 (100.00%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            TLA+H+FLFLGAGEAGTGI
Sbjct:  328 TLADHKFLFLGAGEAGTGI 346

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOC_ORYSJ (NADP-dependent malic enzyme, chloroplastic OS=Oryza sativa subsp. japonica GN=ME6 PE=2 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 5.400e-41
Identity = 54/62 (87.10%), Postives = 59/62 (95.16%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G+RPSACLPIT+DVGTNNEQLL DEFYIGLRQRRATG+EY EL++EFMSAVKQ YGEKVLIQ
Sbjct:  267 GVRPSACLPITIDVGTNNEQLLNDEFYIGLRQRRATGKEYHELMEEFMSAVKQIYGEKVLIQ 328

HSP 2 Score: 55.4546 bits (132), Expect = 5.400e-41
Identity = 24/28 (85.71%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF+LLAKY  +HLVFNDDIQ
Sbjct:  329 FEDFANHNAFDLLAKYSKSHLVFNDDIQ 356

HSP 3 Score: 38.5058 bits (88), Expect = 5.400e-41
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            TLAEH +LFLGAGEAGTGI
Sbjct:  376 TLAEHTYLFLGAGEAGTGI 394

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOX_PHAVU (NADP-dependent malic enzyme OS=Phaseolus vulgaris GN=ME1 PE=2 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 9.143e-41
Identity = 52/62 (83.87%), Postives = 58/62 (93.55%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G+RPS+CLP+T+DVGTNNE+LL DEFYIGLRQRRATGQEYA  LDEFM AVKQNYGEKVL+Q
Sbjct:  217 GVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQRRATGQEYATFLDEFMRAVKQNYGEKVLVQ 278

HSP 2 Score: 54.299 bits (129), Expect = 9.143e-41
Identity = 23/28 (82.14%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF+LL KY ++HLVFNDDIQ
Sbjct:  279 FEDFANHNAFDLLEKYSSSHLVFNDDIQ 306

HSP 3 Score: 38.5058 bits (88), Expect = 9.143e-41
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            TLA+H FLFLGAGEAGTGI
Sbjct:  326 TLADHTFLFLGAGEAGTGI 344

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOX_MESCR (NADP-dependent malic enzyme OS=Mesembryanthemum crystallinum GN=MOD1 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.415e-40
Identity = 48/62 (77.42%), Postives = 58/62 (93.55%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G+ PSACLPIT+DVGTNN++LL DEFYIGL+Q+RATG+EYAE + EFMSAVKQNYGEK+L+Q
Sbjct:  213 GVCPSACLPITLDVGTNNQKLLDDEFYIGLKQKRATGEEYAEFVQEFMSAVKQNYGEKILVQ 274

HSP 2 Score: 57.7658 bits (138), Expect = 4.415e-40
Identity = 26/28 (92.86%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAFELL KY TTHLVFNDDIQ
Sbjct:  275 FEDFANHNAFELLEKYRTTHLVFNDDIQ 302

HSP 3 Score: 39.6614 bits (91), Expect = 4.415e-40
Identity = 17/19 (89.47%), Postives = 19/19 (100.00%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            TLA+H+FLFLGAGEAGTGI
Sbjct:  322 TLADHKFLFLGAGEAGTGI 340

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOC_FLATR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria trinervia GN=MOD1 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 2.254e-38
Identity = 51/63 (80.95%), Postives = 59/63 (93.65%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKD-EFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G+RPSACLPIT+DVGTNNE+LL D EFYIGL+Q+RA GQEYAEL++EFMSAVKQNYGE +LIQ
Sbjct:  275 GVRPSACLPITIDVGTNNEKLLNDDEFYIGLKQKRAAGQEYAELMNEFMSAVKQNYGENLLIQ 337

HSP 2 Score: 56.6102 bits (135), Expect = 2.254e-38
Identity = 25/28 (89.29%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF+LL KY TTHLVFNDDIQ
Sbjct:  338 FEDFANHNAFDLLEKYRTTHLVFNDDIQ 365

HSP 3 Score: 35.039 bits (79), Expect = 2.254e-38
Identity = 15/19 (78.95%), Postives = 18/19 (94.74%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            +LA+ +FLFLGAGEAGTGI
Sbjct:  385 SLADQKFLFLGAGEAGTGI 403

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOC_SOLLC (NADP-dependent malic enzyme, chloroplastic (Fragment) OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 4.961e-38
Identity = 50/62 (80.65%), Postives = 55/62 (88.71%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            GIRPSACLP+T+DVG    + + DEFYIGLRQRRATGQEY+ELLDEFM AVKQNYGEKVLIQ
Sbjct:  203 GIRPSACLPVTIDVG-QTMKFVDDEFYIGLRQRRATGQEYSELLDEFMYAVKQNYGEKVLIQ 263

HSP 2 Score: 59.3066 bits (142), Expect = 4.961e-38
Identity = 26/28 (92.86%), Postives = 27/28 (96.43%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF LLAKYGT+HLVFNDDIQ
Sbjct:  264 FEDFANHNAFNLLAKYGTSHLVFNDDIQ 291

HSP 3 Score: 36.965 bits (84), Expect = 4.961e-38
Identity = 16/19 (84.21%), Postives = 18/19 (94.74%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            +L+EH FLFLGAGEAGTGI
Sbjct:  311 SLSEHTFLFLGAGEAGTGI 329

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOC_MAIZE (NADP-dependent malic enzyme, chloroplastic OS=Zea mays GN=MOD1 PE=1 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 4.032e-37
Identity = 50/62 (80.65%), Postives = 56/62 (90.32%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            G+ PS CLPIT+DVGTNNE LL DEFYIGLRQ+RATG+EY EL++EFMSAVKQ YGEKVLIQ
Sbjct:  264 GVDPSVCLPITIDVGTNNEFLLNDEFYIGLRQKRATGEEYDELIEEFMSAVKQFYGEKVLIQ 325

HSP 2 Score: 53.5286 bits (127), Expect = 4.032e-37
Identity = 23/28 (82.14%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDFANHNAF+LL KY  +HLVFNDDIQ
Sbjct:  326 FEDFANHNAFDLLEKYSKSHLVFNDDIQ 353

HSP 3 Score: 35.4242 bits (80), Expect = 4.032e-37
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 2

Query:  326 TLAEHRFLFLGAGEAGTGI 382
            TLAE  +LFLGAGEAGTGI
Sbjct:  373 TLAEQTYLFLGAGEAGTGI 391

[Close]

BLAST of CF504598 vs. ExPASy Swiss-Prot
Match: MAOM_HUMAN (NAD-dependent malic enzyme, mitochondrial OS=Homo sapiens GN=ME2 PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 4.508e-24
Identity = 35/62 (56.45%), Postives = 44/62 (70.97%), Query Frame = 1

Query:    1 GIRPSACLPITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 186
            GIRP  CLP+ +DVGT+N  LLKD FY+GL Q+R   Q+Y +L+DEFM A+   YG   LIQ
Sbjct:  192 GIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQ 253

HSP 2 Score: 42.3578 bits (98), Expect = 4.508e-24
Identity = 18/28 (64.29%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  192 FEDFANHNAFELLAKYGTTHLVFNDDIQ 275
            FEDF NHNAF  L KY   +  FNDDIQ
Sbjct:  254 FEDFGNHNAFRFLRKYREKYCTFNDDIQ 281

HSP 3 Score: 30.0314 bits (66), Expect = 4.508e-24
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  329 LAEHRFLFLGAGEAGTGI 382
            ++EH+ LFLGAGEA  GI
Sbjct:  302 ISEHKILFLGAGEAALGI 319

The following BLAST results are available for this feature:

BLAST of CF504598 vs. ExPASy Swiss-Prot

BLAST of CF504598 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 137


Match Name	E-value	Identity	Description
MAOX_POPTR	3.464e-44	90.32	NADP-dependent malic enzyme OS=Populus trichocarpa...	[more]
MAOC_FLAPR	8.595e-42	87.10	NADP-dependent malic enzyme, chloroplastic OS=Flav...	[more]
MAOX_VITVI	1.456e-41	82.26	NADP-dependent malic enzyme OS=Vitis vinifera PE=2...	[more]
MAOC_ORYSJ	5.400e-41	87.10	NADP-dependent malic enzyme, chloroplastic OS=Oryz...	[more]
MAOX_PHAVU	9.143e-41	83.87	NADP-dependent malic enzyme OS=Phaseolus vulgaris ...	[more]
MAOX_MESCR	4.415e-40	77.42	NADP-dependent malic enzyme OS=Mesembryanthemum cr...	[more]
MAOC_FLATR	2.254e-38	80.95	NADP-dependent malic enzyme, chloroplastic OS=Flav...	[more]
MAOC_SOLLC	4.961e-38	80.65	NADP-dependent malic enzyme, chloroplastic (Fragme...	[more]
MAOC_MAIZE	4.032e-37	80.65	NADP-dependent malic enzyme, chloroplastic OS=Zea ...	[more]
MAOM_HUMAN	4.508e-24	56.45	NAD-dependent malic enzyme, mitochondrial OS=Homo ...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_120000-087 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_B08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504598 ID=CF504598; Name=CF504598; organism=Citrus sinensis; type=EST; length=383bp
GGCATTCGTCCCTCAGCATGTTTGCCCATAACCGTTGATGTGGGTACAAA
CAATGAGCAATTGTTGAAGGATGAATTCTACATTGGTCTACGACAGAGGA
GGGCAACCGGACAGGAATATGCTGAACTTCTAGATGAATTCATGAGTGCT
GTCAAGCAAAATTATGGAGAAAAAGTTCTTATACAGGTTAGTTTGAGGAT
TTCGCAAACCACAATGCTTTTGAGTTGCTTGCAAAATATGGCACAACTCA
TCTTGTCTTCAATGATGATATACAGCGTCTGTTGTTCTTGCTGGGGTAGT
TGCAGCACTGAAGTTGATTGGTGGCACACTGGCCGAGCATAGATTTTTGT
TTCTTGGAGCTGGGGAGGCTGGGACTGGTATAG