CF504624

Overview
NameCF504624
Unique NameCF504624
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length643
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: KPK19_ARATH (Serine/threonine-protein kinase AtPK19 OS=Arabidopsis thaliana GN=ATPK19 PE=2 SV=2)

HSP 1 Score: 134.035 bits (336), Expect = 6.272e-31
Identity = 66/87 (75.86%), Postives = 75/87 (86.21%), Query Frame = 2
Query:   89 VGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLYHT*DVLS 349
            VG+EDFEVLKVVGQGAF KVYQVR+  T EIYAMKVMRKDKI+EKNHAEYMK+ER+ILTK+DHPFIVQL+YSFQ    LY   D ++
Sbjct:  135 VGIEDFEVLKVVGQGAFGKVYQVRKKDTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTKIDHPFIVQLKYSFQTKYRLYLVLDFIN 221          

HSP 2 Score: 82.0333 bits (201), Expect = 2.801e-15
Identity = 37/40 (92.50%), Postives = 39/40 (97.50%), Query Frame = 3
Query:  522 QTKYRLYLVLDFVNGGHLFFQLYRQGLFREDLARIYTAEI 641
            QTKYRLYLVLDF+NGGHLFFQLY QGLFREDLAR+YTAEI
Sbjct:  208 QTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEI 247          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: KPK1_ARATH (Serine/threonine-protein kinase AtPK1/AtPK6 OS=Arabidopsis thaliana GN=ATPK1 PE=1 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.545e-29
Identity = 63/87 (72.41%), Postives = 74/87 (85.06%), Query Frame = 2
Query:   89 VGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLYHT*DVLS 349
            VG++DFEV+KVVG+GAF KVYQVR+  T EIYAMKVMRKD IMEKNHAEYMK+ER+ILTK+DHPFIVQL+YSFQ    LY   D ++
Sbjct:  129 VGIDDFEVMKVVGKGAFGKVYQVRKKETSEIYAMKVMRKDHIMEKNHAEYMKAERDILTKIDHPFIVQLKYSFQTKYRLYLVLDFIN 215          

HSP 2 Score: 82.0333 bits (201), Expect = 2.801e-15
Identity = 37/40 (92.50%), Postives = 39/40 (97.50%), Query Frame = 3
Query:  522 QTKYRLYLVLDFVNGGHLFFQLYRQGLFREDLARIYTAEI 641
            QTKYRLYLVLDF+NGGHLFFQLY QGLFREDLAR+YTAEI
Sbjct:  202 QTKYRLYLVLDFINGGHLFFQLYHQGLFREDLARVYTAEI 241          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: KRAC_DICDI (RAC family serine/threonine-protein kinase homolog OS=Dictyostelium discoideum GN=pkbA PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 4.981e-20
Identity = 48/93 (51.61%), Postives = 65/93 (69.89%), Query Frame = 2
Query:   71 PSENECVGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLYHT*DVLS 349
            P ++E VG+ DFE+L +VG+G+F KV QVR+  T E+YAMKV+ K  I+E N  E+  SERNIL K++HPF+V L YSFQ    LY   D ++
Sbjct:  109 PKKSEKVGVADFELLNLVGKGSFGKVIQVRKKDTGEVYAMKVLSKKHIVEHNEVEHTLSERNILQKINHPFLVNLNYSFQTEDKLYFILDYVN 201          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: YPK1_YEAST (Serine/threonine-protein kinase YPK1 OS=Saccharomyces cerevisiae GN=YPK1 PE=1 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 2.093e-18
Identity = 45/101 (44.55%), Postives = 68/101 (67.33%), Query Frame = 2
Query:   26 VDAALGKPTGHIDNLPSENECVGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLY 328
            +D  LGK    ID  PS N+ + ++DF++LKV+G+G+F KV QVR+  T ++YA+K +RK  I+ K+   +  +ER +L +VD PFIV L++SFQ    LY
Sbjct:  321 LDNGLGKINISIDYKPSRNKPLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKVYALKAIRKSYIVSKSEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLY 421          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: YPK2_YEAST (Serine/threonine-protein kinase YPK2/YKR2 OS=Saccharomyces cerevisiae GN=YPK2 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 6.732e-17
Identity = 43/101 (42.57%), Postives = 67/101 (66.34%), Query Frame = 2
Query:   26 VDAALGKPTGHIDNLPSENECVGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLY 328
            ++   GK    +D  PS+N+ + ++DF++LKV+G+G+F KV QVR+  T +IYA+K +RK  I+ K    +  +ER +L +VD PFIV L++SFQ    LY
Sbjct:  318 LERGYGKLNITVDYKPSKNKPLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKIYALKALRKAYIVSKCEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLY 418          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: PK2_DICDI (Protein kinase 2 OS=Dictyostelium discoideum GN=pkgB PE=1 SV=2)

HSP 1 Score: 83.1889 bits (204), Expect = 1.270e-15
Identity = 39/84 (46.43%), Postives = 57/84 (67.86%), Query Frame = 2
Query:   98 EDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLYHT*DVLS 349
            +DFE+L V+G+G+F KV QV++ G  +I+AMKV+RKD I+ +    + KSE+ IL  + HPFIV L Y+FQ    LY   D ++
Sbjct:  151 DDFELLNVIGKGSFGKVMQVKKKGEDKIFAMKVLRKDAIIARKQVNHTKSEKTILQCISHPFIVNLHYAFQTKDKLYMVLDFVN 234          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: SGK1_CAEEL (Serine/threonine-protein kinase sgk-1 OS=Caenorhabditis elegans GN=sgk-1 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.828e-15
Identity = 41/99 (41.41%), Postives = 62/99 (62.63%), Query Frame = 2
Query:   56 HIDNLPSENECVGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNIL-TKVDHPFIVQLRYSFQVMSYLYHT*DVLS 349
            ++D  PSE +     DF+ L  +G+G+F +VYQVR   T +IYAMK++ K+ I +KN  +++ +ERN+L     HPF+V L +SFQ    LY   D L+
Sbjct:  119 NVDLGPSERKTATANDFDYLTTIGKGSFGRVYQVRHKETKKIYAMKILSKEHIRKKNEVKHVMAERNVLINNFKHPFLVSLHFSFQNKEKLYFVLDHLN 217          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: TRC_DROPS (Serine/threonine-protein kinase tricorner OS=Drosophila pseudoobscura pseudoobscura GN=trc PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.075e-14
Identity = 36/80 (45.00%), Postives = 58/80 (72.50%), Query Frame = 2
Query:   89 VGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLY 328
            +G+EDFE LKV+G+GAF +V  V++  T  +YAMKV+RK  ++EK    ++++ER++L + DH ++V++ YSFQ    LY
Sbjct:   87 LGVEDFEALKVIGRGAFGEVRLVQKKDTGHVYAMKVLRKADMLEKEQVAHVRAERDVLVEADHQWVVKMYYSFQDQVNLY 166          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: TRC_DROME (Serine/threonine-protein kinase tricorner OS=Drosophila melanogaster GN=trc PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.833e-14
Identity = 36/80 (45.00%), Postives = 58/80 (72.50%), Query Frame = 2
Query:   89 VGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLY 328
            +G+EDFE LKV+G+GAF +V  V++  T  +YAMKV+RK  ++EK    ++++ER++L + DH ++V++ YSFQ    LY
Sbjct:   88 LGVEDFEALKVIGRGAFGEVRLVQKKDTGHVYAMKVLRKADMLEKEQVAHVRAERDVLVEADHQWVVKMYYSFQDPVNLY 167          
BLAST of CF504624 vs. ExPASy Swiss-Prot
Match: GAD8_SCHPO (Serine/threonine-protein kinase gad8 OS=Schizosaccharomyces pombe GN=gad8 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.833e-14
Identity = 37/86 (43.02%), Postives = 61/86 (70.93%), Query Frame = 2
Query:   71 PSENECVGLEDFEVLKVVGQGAFAKVYQVRRIGTLEIYAMKVMRKDKIMEKNHAEYMKSERNILTKVDHPFIVQLRYSFQVMSYLY 328
            P+++  + ++ FE+LKVVG+G+F KV QVR+  T  IYA+K M+K  I+ ++  ++  +ER +L +V++PFIV L++SFQ    LY
Sbjct:  219 PNQSTPLTIDAFELLKVVGKGSFGKVMQVRKRDTSRIYALKTMKKAHIVSRSEVDHTLAERTVLAQVNNPFIVPLKFSFQSPGKLY 304          
The following BLAST results are available for this feature:
BLAST of CF504624 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 114
Match NameE-valueIdentityDescription
KPK19_ARATH6.272e-3175.86Serine/threonine-protein kinase AtPK19 OS=Arabidop... [more]
KPK1_ARATH1.545e-2972.41Serine/threonine-protein kinase AtPK1/AtPK6 OS=Ara... [more]
KRAC_DICDI4.981e-2051.61RAC family serine/threonine-protein kinase homolog... [more]
YPK1_YEAST2.093e-1844.55Serine/threonine-protein kinase YPK1 OS=Saccharomy... [more]
YPK2_YEAST6.732e-1742.57Serine/threonine-protein kinase YPK2/YKR2 OS=Sacch... [more]
PK2_DICDI1.270e-1546.43Protein kinase 2 OS=Dictyostelium discoideum GN=pk... [more]
SGK1_CAEEL2.828e-1541.41Serine/threonine-protein kinase sgk-1 OS=Caenorhab... [more]
TRC_DROPS1.075e-1445.00Serine/threonine-protein kinase tricorner OS=Droso... [more]
TRC_DROME1.833e-1445.00Serine/threonine-protein kinase tricorner OS=Droso... [more]
GAD8_SCHPO1.833e-1443.02Serine/threonine-protein kinase gad8 OS=Schizosacc... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-113 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-1_D12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504624 ID=CF504624; Name=CF504624; organism=Citrus sinensis; type=EST; length=643bp
GGAAGGGCCTAACAACCTATCTGTTGTTGATGCTGCCCTGGGGAAACCTA
CCGGGCACATAGATAATCTTCCTTCTGAAAACGAGTGTGTAGGACTTGAA
GATTTTGAGGTTTTGAAGGTTGTTGGCCAAGGTGCTTTTGCAAAAGTATA
CCAGGTGAGGAGGATCGGTACTTTAGAAATTTATGCAATGAAGGTCATGC
GCAAGGACAAGATTATGGAGAAAAATCATGCGGAATACATGAAATCTGAG
AGGAATATACTGACGAAAGTAGATCACCCCTTCATTGTGCAGCTCAGATA
CTCATTTCAAGTAATGTCTTATCTTTATCACACATAAGATGTTTTGTCTT
TTTAACCCTGCACCGGACTATGTGAATTCCATGCACTTTGCTTTGCCTTC
ATGATTCAGTTAATTAGTTAGTGATCTCAAGATTAGTTTGATCATGATAG
CAGATTTCCATTGCCCTACTTATGGAGCTTTGCATATTGTATACAGCAGT
TATATAAATTAATCTGTTCTGCAGACCAAATATAGACTGTACCTTGTGCT
TGATTTTGTCAATGGCGGCCACCTTTTCTTTCAGCTCTATCGCCAAGGCT
TGTTCAGGGAGGATTTGGCACGTATATATACTGCTGAGATTGT
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