CF504668

Overview
NameCF504668
Unique NameCF504668
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length185
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACOC_CUCMA (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 4.294e-27
Identity = 58/61 (95.08%), Postives = 60/61 (98.36%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF+GDGM ELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETV+M
Sbjct:  276 GVVGKFVEFYGDGMEELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVSM 336          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACOC_CUCMC (Aconitate hydratase (Fragment) OS=Cucumis melo var. conomon GN=ACO PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 5.608e-27
Identity = 56/61 (91.80%), Postives = 60/61 (98.36%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF+G+GMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGR DET++M
Sbjct:  142 GVVGKFVEFYGEGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRKDETISM 202          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACOC_ORYSJ (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 7.324e-27
Identity = 57/61 (93.44%), Postives = 60/61 (98.36%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF+G+GMG+LSLADRATIANMSPEYGATMGFFPVDHVTL YLKLTGRSDETVAM
Sbjct:  276 GVVGKFVEFYGEGMGKLSLADRATIANMSPEYGATMGFFPVDHVTLDYLKLTGRSDETVAM 336          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACO1_ARATH (Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2)

HSP 1 Score: 118.242 bits (295), Expect = 1.249e-26
Identity = 56/61 (91.80%), Postives = 60/61 (98.36%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEFHG+GM ELSLADRATIANMSPEYGATMGFFPVDHVTLQYL+LTGRSD+TV+M
Sbjct:  276 GVVGKFVEFHGEGMRELSLADRATIANMSPEYGATMGFFPVDHVTLQYLRLTGRSDDTVSM 336          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACO3M_ARATH (Aconitate hydratase 3, mitochondrial OS=Arabidopsis thaliana GN=ACO3 PE=1 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 1.632e-26
Identity = 56/61 (91.80%), Postives = 60/61 (98.36%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF+G+GM ELSLADRATIANMSPEYGATMGFFPVDHVTL+YLKLTGRSDETV+M
Sbjct:  373 GVVGKFVEFYGEGMSELSLADRATIANMSPEYGATMGFFPVDHVTLEYLKLTGRSDETVSM 433          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACO2M_ARATH (Aconitate hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=ACO2 PE=1 SV=2)

HSP 1 Score: 117.087 bits (292), Expect = 2.783e-26
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF+G+GM  LSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM
Sbjct:  368 GVVGKFVEFYGNGMSGLSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 428          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACON1_PSEAE (Aconitate hydratase 1 OS=Pseudomonas aeruginosa GN=acnA PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.892e-20
Identity = 44/61 (72.13%), Postives = 53/61 (86.89%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF+GDG+ +L LADRATIANM+PEYGAT GFFPVD +TL YL+L+GR + TV +
Sbjct:  279 GVVGKFVEFYGDGLADLPLADRATIANMAPEYGATCGFFPVDEITLGYLRLSGRPESTVKL 339          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACOC_SOLTU (Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.892e-20
Identity = 46/54 (85.19%), Postives = 49/54 (90.74%), Query Frame = 3
Query:   24 EFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            EF+G GM  LSLADRATIANM+PEYGATMGFFPVDHVTL+YLKLTGRSDE V M
Sbjct:    1 EFYGGGMSGLSLADRATIANMAPEYGATMGFFPVDHVTLEYLKLTGRSDEIVGM 54          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACON1_ECOLI (Aconitate hydratase 1 OS=Escherichia coli (strain K12) GN=acnA PE=1 SV=3)

HSP 1 Score: 96.2857 bits (238), Expect = 5.084e-20
Identity = 44/61 (72.13%), Postives = 53/61 (86.89%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF+GDG+  L LADRATIANMSPEYGAT GFFP+D VTL Y++L+GRS++ V +
Sbjct:  279 GVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVEL 339          
BLAST of CF504668 vs. ExPASy Swiss-Prot
Match: ACON_STAAW (Aconitate hydratase OS=Staphylococcus aureus (strain MW2) GN=acnA PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.932e-19
Identity = 45/61 (73.77%), Postives = 52/61 (85.25%), Query Frame = 3
Query:    3 GVVGKFVEFHGDGMGELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETVAM 185
            GVVGKFVEF G G+  L LADRATIANM+PEYGAT GFFPVD  +L+Y+KLTGRSDE +A+
Sbjct:  279 GVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSDEHIAL 339          
The following BLAST results are available for this feature:
BLAST of CF504668 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48
Match NameE-valueIdentityDescription
ACOC_CUCMA4.294e-2795.08Aconitate hydratase, cytoplasmic OS=Cucurbita maxi... [more]
ACOC_CUCMC5.608e-2791.80Aconitate hydratase (Fragment) OS=Cucumis melo var... [more]
ACOC_ORYSJ7.324e-2793.44Putative aconitate hydratase, cytoplasmic OS=Oryza... [more]
ACO1_ARATH1.249e-2691.80Aconitate hydratase 1 OS=Arabidopsis thaliana GN=A... [more]
ACO3M_ARATH1.632e-2691.80Aconitate hydratase 3, mitochondrial OS=Arabidopsi... [more]
ACO2M_ARATH2.783e-2693.44Aconitate hydratase 2, mitochondrial OS=Arabidopsi... [more]
ACON1_PSEAE3.892e-2072.13Aconitate hydratase 1 OS=Pseudomonas aeruginosa GN... [more]
ACOC_SOLTU3.892e-2085.19Aconitate hydratase, cytoplasmic (Fragment) OS=Sol... [more]
ACON1_ECOLI5.084e-2072.13Aconitate hydratase 1 OS=Escherichia coli (strain ... [more]
ACON_STAAW1.932e-1973.77Aconitate hydratase OS=Staphylococcus aureus (stra... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_120000-157 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-20_A02 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF504668 ID=CF504668; Name=CF504668; organism=Citrus sinensis; type=EST; length=185bp
ATGGTGTTGTTGGAAAATTTGTTGAGTTCCATGGGGATGGTATGGGTGAA
CTTTCACTTGCTGACAGAGCTACCATTGCTAATATGTCTCCCGAGTATGG
AGCAACCATGGGGTTCTTTCCTGTGGATCATGTTACTTTACAATATCTCA
AATTGACTGGAAGGAGTGATGAGACAGTGGCAATG
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