CF504898 | Citrus Genome Database

Overview


Name	CF504898
Unique Name	CF504898
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	252

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX4_ARATH (Lipoxygenase 4, chloroplastic OS=Arabidopsis thaliana GN=LOX4 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 6.650e-26
Identity = 37/47 (78.72%), Postives = 42/47 (89.36%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAYKN-WRFDKEGLPADLIRR 228
            RQ LI+ADGVIESCFT G+Y +E+S+AAYKN WRFD EGLPADLIRR
Sbjct:  629 RQTLISADGVIESCFTAGQYGLEISSAAYKNKWRFDMEGLPADLIRR 675

HSP 2 Score: 60.4622 bits (145), Expect = 6.650e-26
Identity = 27/29 (93.10%), Postives = 29/29 (100.00%), Query Frame = 3

Query:    3 AHRQLSAMHPIYKLLDPHMRYTLEINALA 89
            AHRQLSAMHPI+KLLDPHMRYTLEINA+A
Sbjct:  600 AHRQLSAMHPIFKLLDPHMRYTLEINAVA 628

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX3_ARATH (Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana GN=LOX3 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 7.124e-25
Identity = 37/47 (78.72%), Postives = 40/47 (85.11%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAYK-NWRFDKEGLPADLIRR 228
            RQ+LI+ADGVIE  FT G Y MEMSAAAYK +WRFD EGLPADLIRR
Sbjct:  622 RQSLISADGVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADLIRR 668

HSP 2 Score: 61.6178 bits (148), Expect = 7.124e-25
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3

Query:    3 AHRQLSAMHPIYKLLDPHMRYTLEINALA 89
            AHRQLSAMHPI+KLLDPHMRYTLEINALA
Sbjct:  593 AHRQLSAMHPIFKLLDPHMRYTLEINALA 621

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX6_ORYSJ (Probable lipoxygenase 6 OS=Oryza sativa subsp. japonica GN=Os03g0179900 PE=2 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 1.679e-23
Identity = 36/47 (76.60%), Postives = 40/47 (85.11%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAYKN-WRFDKEGLPADLIRR 228
            RQ+LINADGVIESCFTPG    E+SAA Y+N WRFD EGLP+DLIRR
Sbjct:  617 RQSLINADGVIESCFTPGPVSGEISAAYYRNHWRFDLEGLPSDLIRR 663

HSP 2 Score: 56.225 bits (134), Expect = 1.679e-23
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 3

Query:    3 AHRQLSAMHPIYKLLDPHMRYTLEINALA 89
            AHR +SAMHPI+KLL PHMRYTLEINALA
Sbjct:  588 AHRHMSAMHPIFKLLHPHMRYTLEINALA 616

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX6_ARATH (Lipoxygenase 6, choloroplastic OS=Arabidopsis thaliana GN=LOX6 PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 2.185e-23
Identity = 34/47 (72.34%), Postives = 42/47 (89.36%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAYKN-WRFDKEGLPADLIRR 228
            R++LIN  G+IESCFTPG+Y ME+S+AAYK+ WRFD EGLPADL+RR
Sbjct:  619 RKSLINGGGIIESCFTPGKYAMELSSAAYKSMWRFDMEGLPADLVRR 665

HSP 2 Score: 51.9878 bits (123), Expect = 2.185e-23
Identity = 23/28 (82.14%), Postives = 25/28 (89.29%), Query Frame = 3

Query:    6 HRQLSAMHPIYKLLDPHMRYTLEINALA 89
            +RQLS MHP+YKLL PHMRYTLEINA A
Sbjct:  591 NRQLSTMHPVYKLLHPHMRYTLEINARA 618

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX5_ORYSJ (Putative lipoxygenase 5 OS=Oryza sativa subsp. japonica GN=Os04g0447100 PE=3 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 2.186e-23
Identity = 36/47 (76.60%), Postives = 38/47 (80.85%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAYKN-WRFDKEGLPADLIRR 228
            RQ LIN DGVIES FTPG  CMEMSA AY+  WR D+EGLPADLIRR
Sbjct:  603 RQILINGDGVIESGFTPGNVCMEMSAFAYRELWRLDQEGLPADLIRR 649

HSP 2 Score: 56.6102 bits (135), Expect = 2.186e-23
Identity = 25/29 (86.21%), Postives = 28/29 (96.55%), Query Frame = 3

Query:    3 AHRQLSAMHPIYKLLDPHMRYTLEINALA 89
            AHRQ+SAMHPI+KLL PHMRYTL+INALA
Sbjct:  574 AHRQMSAMHPIFKLLKPHMRYTLKINALA 602

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.242e-21
Identity = 30/47 (63.83%), Postives = 40/47 (85.11%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAY-KNWRFDKEGLPADLIRR 228
            RQ+L+N  G+IE+CF PG+Y +E+S+A Y K WRFD+EGLPADLI+R
Sbjct:  598 RQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKR 644

HSP 2 Score: 51.6026 bits (122), Expect = 3.242e-21
Identity = 23/29 (79.31%), Postives = 26/29 (89.66%), Query Frame = 3

Query:    3 AHRQLSAMHPIYKLLDPHMRYTLEINALA 89
            A+RQLSAMHPIY+LL PH RYT+EINA A
Sbjct:  569 ANRQLSAMHPIYRLLHPHFRYTMEINARA 597

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX22_HORVU (Lipoxygenase 2.2, chloroplastic OS=Hordeum vulgare GN=LOX2.2 PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.449e-17
Identity = 30/47 (63.83%), Postives = 35/47 (74.47%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAY-KNWRFDKEGLPADLIRR 228
            R  LI ADG+IE  F+PG + ME+S+AAY K WRFD E LP DLIRR
Sbjct:  632 RGMLICADGIIEKTFSPGEFSMEISSAAYDKQWRFDMEALPEDLIRR 678

HSP 2 Score: 48.1358 bits (113), Expect = 1.449e-17
Identity = 21/29 (72.41%), Postives = 25/29 (86.21%), Query Frame = 3

Query:    3 AHRQLSAMHPIYKLLDPHMRYTLEINALA 89
            A+RQLS MHPIY+LL PH R+T+EINA A
Sbjct:  603 ANRQLSQMHPIYRLLHPHFRFTMEINAQA 631

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX2_SOYBN (Seed lipoxygenase-2 OS=Glycine max GN=LOX1.2 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.451e-17
Identity = 28/46 (60.87%), Postives = 36/46 (78.26%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAYKNWRFDKEGLPADLIRR 228
            RQ+LINADG+IE  F P ++ +EMS+A YKNW F  + LPADLI+R
Sbjct:  571 RQSLINADGIIEKSFLPSKHSVEMSSAVYKNWVFTDQALPADLIKR 616

HSP 2 Score: 44.2838 bits (103), Expect = 1.451e-17
Identity = 20/28 (71.43%), Postives = 23/28 (82.14%), Query Frame = 3

Query:    6 HRQLSAMHPIYKLLDPHMRYTLEINALA 89
            +R LSA+HPIYKLL PH R T+ INALA
Sbjct:  543 NRHLSALHPIYKLLTPHYRDTMNINALA 570

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOX1_SOYBN (Seed lipoxygenase-1 OS=Glycine max GN=LOX1.1 PE=1 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 1.452e-17
Identity = 28/46 (60.87%), Postives = 37/46 (80.43%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAYKNWRFDKEGLPADLIRR 228
            RQ+LINA+G+IE+ F P +Y +EMS+A YKNW F  + LPADLI+R
Sbjct:  543 RQSLINANGIIETTFLPSKYSVEMSSAVYKNWVFTDQALPADLIKR 588

HSP 2 Score: 43.8986 bits (102), Expect = 1.452e-17
Identity = 19/28 (67.86%), Postives = 21/28 (75.00%), Query Frame = 3

Query:    6 HRQLSAMHPIYKLLDPHMRYTLEINALA 89
            HR LS +HPIYKLL PH R  + INALA
Sbjct:  515 HRHLSVLHPIYKLLTPHYRNNMNINALA 542

[Close]

BLAST of CF504898 vs. ExPASy Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 2.448e-17
Identity = 28/47 (59.57%), Postives = 35/47 (74.47%), Query Frame = 1

Query:   91 RQNLINADGVIESCFTPGRYCMEMSAAAY-KNWRFDKEGLPADLIRR 228
            R  LI+A G+IE  F+P +Y ME+S+ AY K WRFD E LPADL+RR
Sbjct:  642 RSRLISAAGIIELSFSPQKYSMELSSVAYDKLWRFDMEALPADLVRR 688

HSP 2 Score: 49.6766 bits (117), Expect = 2.448e-17
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 3

Query:    3 AHRQLSAMHPIYKLLDPHMRYTLEINALA 89
            A+RQLS MHPIY+LL PH RYT+ INALA
Sbjct:  613 ANRQLSEMHPIYQLLHPHFRYTMRINALA 641

The following BLAST results are available for this feature:

BLAST of CF504898 vs. ExPASy Swiss-Prot

BLAST of CF504898 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 30


Match Name	E-value	Identity	Description
LOX4_ARATH	6.650e-26	78.72	Lipoxygenase 4, chloroplastic OS=Arabidopsis thali...	[more]
LOX3_ARATH	7.124e-25	78.72	Lipoxygenase 3, chloroplastic OS=Arabidopsis thali...	[more]
LOX6_ORYSJ	1.679e-23	76.60	Probable lipoxygenase 6 OS=Oryza sativa subsp. jap...	[more]
LOX6_ARATH	2.185e-23	72.34	Lipoxygenase 6, choloroplastic OS=Arabidopsis thal...	[more]
LOX5_ORYSJ	2.186e-23	76.60	Putative lipoxygenase 5 OS=Oryza sativa subsp. jap...	[more]
LOX2_ARATH	3.242e-21	63.83	Lipoxygenase 2, chloroplastic OS=Arabidopsis thali...	[more]
LOX22_HORVU	1.449e-17	63.83	Lipoxygenase 2.2, chloroplastic OS=Hordeum vulgare...	[more]
LOX2_SOYBN	1.451e-17	60.87	Seed lipoxygenase-2 OS=Glycine max GN=LOX1.2 PE=2 ...	[more]
LOX1_SOYBN	1.452e-17	60.87	Seed lipoxygenase-1 OS=Glycine max GN=LOX1.1 PE=1 ...	[more]
LOXC2_ORYSJ	2.448e-17	59.57	Probable lipoxygenase 8, chloroplastic OS=Oryza sa...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_120000-390 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-23_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF504898 ID=CF504898; Name=CF504898; organism=Citrus sinensis; type=EST; length=252bp
CAGCACATAGACAATTGAGTGCAATGCACCCAATTTACAAGCTTTTGGAT
CCACATATGAGATACACATTGGAGATTAATGCTCTAGCCTAGGCAAAATT
TAATCAATGCCGATGGTGTGATTGAGTCTTGCTTCACTCCTGGCCGCTAC
TGCATGGAAATGAGCGCCGCCGCGTACAAAAATTGGCGGTTTGACAAGGA
AGGCCTCCCCGCAGATCTCATCCGCAGGTAAACACTACTGAATAATTACC
TA