CF509545

Overview
NameCF509545
Unique NameCF509545
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length378
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3326 contig Csv1_Contig3326:33..408. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_SOYBN (DNA-directed RNA polymerase II subunit RPB7 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 5.994e-52
Identity = 79/88 (89.77%), Postives = 83/88 (94.32%), Query Frame = 2 
Query:  113 KMLRAPCSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            K +   CSGRHGFVVA+T +ENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVV+MVNKMGFFAEAGPVQIFVSNHLIPDDMEF
Sbjct:   32 KDVEGTCSGRHGFVVAVTGIENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 119

HSP 2 Score: 48.9062 bits (115), Expect = 5.994e-52
Identity = 20/25 (80.00%), Postives = 25/25 (100.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            LHPR+FGRNLR++++SKLMKDVEGT
Sbjct:   13 LHPRYFGRNLRDNLVSKLMKDVEGT 37

HSP 3 Score: 28.8758 bits (63), Expect = 5.994e-52
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:   18 MFFHIVLERNMQ 53
            MFFHIVLERNMQ
Sbjct:    1 MFFHIVLERNMQ 12
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_ARATH (DNA-directed RNA polymerase II subunit RPB7 OS=Arabidopsis thaliana GN=RPB19 PE=1 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 1.160e-49
Identity = 76/88 (86.36%), Postives = 81/88 (92.05%), Query Frame = 2 
Query:  113 KMLRAPCSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            K +   CSGRHGFVVAIT ++ IGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVV++VNKMGFFAEAGPVQIFVS HLIPDDMEF
Sbjct:   32 KDVEGTCSGRHGFVVAITGIDTIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEF 119

HSP 2 Score: 47.7506 bits (112), Expect = 1.160e-49
Identity = 20/25 (80.00%), Postives = 24/25 (96.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            LHPR FGRNL+E+++SKLMKDVEGT
Sbjct:   13 LHPRFFGRNLKENLVSKLMKDVEGT 37

HSP 3 Score: 28.8758 bits (63), Expect = 1.160e-49
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = 3 
Query:   18 MFFHIVLERNMQ 53
            MFFHIVLERNMQ
Sbjct:    1 MFFHIVLERNMQ 12
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_RAT (DNA-directed RNA polymerase II subunit RPB7 OS=Rattus norvegicus GN=Polr2g PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.500e-29
Identity = 47/82 (57.32%), Postives = 68/82 (82.93%), Query Frame = 2 
Query:  131 CSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            C+G++GFV+A+T+++NIG G+I+ G GFV +PVKY+ +VFRPFKGE+++AVV+ VNK+G F E GP+  F+S H IP +MEF
Sbjct:   38 CTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEF 119

HSP 2 Score: 32.3426 bits (72), Expect = 2.500e-29
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            LHPR+FG NL   +  KL  +VEGT
Sbjct:   13 LHPRYFGPNLLNTVKQKLFTEVEGT 37
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_MOUSE (DNA-directed RNA polymerase II subunit RPB7 OS=Mus musculus GN=Polr2g PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.500e-29
Identity = 47/82 (57.32%), Postives = 68/82 (82.93%), Query Frame = 2 
Query:  131 CSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            C+G++GFV+A+T+++NIG G+I+ G GFV +PVKY+ +VFRPFKGE+++AVV+ VNK+G F E GP+  F+S H IP +MEF
Sbjct:   38 CTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEF 119

HSP 2 Score: 32.3426 bits (72), Expect = 2.500e-29
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            LHPR+FG NL   +  KL  +VEGT
Sbjct:   13 LHPRYFGPNLLNTVKQKLFTEVEGT 37
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_HUMAN (DNA-directed RNA polymerase II subunit RPB7 OS=Homo sapiens GN=POLR2G PE=1 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.500e-29
Identity = 47/82 (57.32%), Postives = 68/82 (82.93%), Query Frame = 2 
Query:  131 CSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            C+G++GFV+A+T+++NIG G+I+ G GFV +PVKY+ +VFRPFKGE+++AVV+ VNK+G F E GP+  F+S H IP +MEF
Sbjct:   38 CTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEF 119

HSP 2 Score: 32.3426 bits (72), Expect = 2.500e-29
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            LHPR+FG NL   +  KL  +VEGT
Sbjct:   13 LHPRYFGPNLLNTVKQKLFTEVEGT 37
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_DANRE (DNA-directed RNA polymerase II subunit RPB7 OS=Danio rerio GN=polr2g PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.500e-29
Identity = 47/82 (57.32%), Postives = 68/82 (82.93%), Query Frame = 2 
Query:  131 CSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            C+G++GFV+A+T+++NIG G+I+ G GFV +PVKY+ +VFRPFKGE+++AVV+ VNK+G F E GP+  F+S H IP +MEF
Sbjct:   38 CTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEF 119

HSP 2 Score: 32.3426 bits (72), Expect = 2.500e-29
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            LHPR+FG NL   +  KL  +VEGT
Sbjct:   13 LHPRYFGPNLLNTVKQKLFTEVEGT 37
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_BOVIN (DNA-directed RNA polymerase II subunit RPB7 OS=Bos taurus GN=POLR2G PE=2 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.500e-29
Identity = 47/82 (57.32%), Postives = 68/82 (82.93%), Query Frame = 2 
Query:  131 CSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            C+G++GFV+A+T+++NIG G+I+ G GFV +PVKY+ +VFRPFKGE+++AVV+ VNK+G F E GP+  F+S H IP +MEF
Sbjct:   38 CTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEF 119

HSP 2 Score: 32.3426 bits (72), Expect = 2.500e-29
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            LHPR+FG NL   +  KL  +VEGT
Sbjct:   13 LHPRYFGPNLLNTVKQKLFTEVEGT 37
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_SCHPO (DNA-directed RNA polymerase II subunit rpb7 OS=Schizosaccharomyces pombe GN=rpb7 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 4.326e-25
Identity = 41/84 (48.81%), Postives = 65/84 (77.38%), Query Frame = 2 
Query:  131 CSGRHGFVVAI--TSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            CSG++G+++ +  ++  +I KG +  G GF  F VKY+ V++RPF+GE+++A+V+ VNKMGFFA  GP+ +FVS+HL+P DM+F
Sbjct:   39 CSGQYGYIICVLDSNTIDIDKGRVVPGQGFAEFEVKYRAVLWRPFRGEVVDAIVTTVNKMGFFANIGPLNVFVSSHLVPPDMKF 122

HSP 2 Score: 36.1946 bits (82), Expect = 4.326e-25
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 1 
Query:   40 SETCSLHPRHFGRNLREHIISKLMKDVEGT 129
            S T SLHP +FG  +++++ +KL+ DVEGT
Sbjct:    9 SLTISLHPSYFGPRMQDYLKAKLLADVEGT 38
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_DICDI (DNA-directed RNA polymerase II subunit rpb7 OS=Dictyostelium discoideum GN=polr2g PE=3 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 5.630e-25
Identity = 45/82 (54.88%), Postives = 65/82 (79.27%), Query Frame = 2 
Query:  131 CSGRHGFVVAITSVENIGKGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            C+GR+GF++ ITSV+ + KG + + +G+V F VKY+ ++F+PFKGE+L+A+V+ V  +GFFAEAGP+ IFVS  LIP DM F
Sbjct:   38 CTGRYGFIITITSVDFLSKGKVLESSGYVVFNVKYKAIIFKPFKGEVLDAIVTKVTNLGFFAEAGPLSIFVSTQLIPSDMIF 119

HSP 2 Score: 26.1794 bits (56), Expect = 5.630e-25
Identity = 11/25 (44.00%), Postives = 15/25 (60.00%), Query Frame = 1 
Query:   55 LHPRHFGRNLREHIISKLMKDVEGT 129
            +HP+H G NL      +L  +VEGT
Sbjct:   13 MHPKHCGPNLFTIATQQLYSEVEGT 37
[Close]
BLAST of CF509545 vs. ExPASy Swiss-Prot
Match: RPB7_YEAST (DNA-directed RNA polymerase II subunit RPB7 OS=Saccharomyces cerevisiae GN=RPB7 PE=1 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.695e-16
Identity = 32/84 (38.10%), Postives = 54/84 (64.29%), Query Frame = 2 
Query:  131 CSGRHGFVVAITSVENIG--KGLIRDGTGFVTFPVKYQCVVFRPFKGEILEAVVSMVNKMGFFAEAGPVQIFVSNHLIPDDMEF 376
            C+G+ G+++ +   +NI   +G I    G   F VKY+ VVF+PFKGE+++  V   ++ GF  + GP+++FV+ HL+P D+ F
Sbjct:   38 CTGKFGYILCVLDYDNIDIQRGRILPTDGSAEFNVKYRAVVFKPFKGEVVDGTVVSCSQHGFEVQVGPMKVFVTKHLMPQDLTF 121

HSP 2 Score: 30.0314 bits (66), Expect = 2.695e-16
Identity = 10/26 (38.46%), Postives = 21/26 (80.77%), Query Frame = 1 
Query:   52 SLHPRHFGRNLREHIISKLMKDVEGT 129
            +LHP  FG  +++++ +KL+++VEG+
Sbjct:   12 TLHPSFFGPRMKQYLKTKLLEEVEGS 37
The following BLAST results are available for this feature:
BLAST of CF509545 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 10
Match NameE-valueIdentityDescription
RPB7_SOYBN5.994e-5289.77DNA-directed RNA polymerase II subunit RPB7 OS=Gly... [more]
RPB7_ARATH1.160e-4986.36DNA-directed RNA polymerase II subunit RPB7 OS=Ara... [more]
RPB7_RAT2.500e-2957.32DNA-directed RNA polymerase II subunit RPB7 OS=Rat... [more]
RPB7_MOUSE2.500e-2957.32DNA-directed RNA polymerase II subunit RPB7 OS=Mus... [more]
RPB7_HUMAN2.500e-2957.32DNA-directed RNA polymerase II subunit RPB7 OS=Hom... [more]
RPB7_DANRE2.500e-2957.32DNA-directed RNA polymerase II subunit RPB7 OS=Dan... [more]
RPB7_BOVIN2.500e-2957.32DNA-directed RNA polymerase II subunit RPB7 OS=Bos... [more]
RPB7_SCHPO4.326e-2548.81DNA-directed RNA polymerase II subunit rpb7 OS=Sch... [more]
RPB7_DICDI5.630e-2554.88DNA-directed RNA polymerase II subunit rpb7 OS=Dic... [more]
RPB7_YEAST2.695e-1638.10DNA-directed RNA polymerase II subunit RPB7 OS=Sac... [more]
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Properties
Property NameValue
Genbank descriptionUSDA-FP_125000-053 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-79_E01 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF509545 ID=CF509545; Name=CF509545; organism=Citrus sinensis; type=EST; length=378bp
AATTATCAGACGAAAAGATGTTCTTTCACATAGTGCTGGAGCGAAACATG
CAGCCTGCATCCTCGCCACTTTGGTCGTAATCTTCGCGAACACATCATCT
CCAAGCTCATGAAAGATGTTGAGGGCACCTTGCAGTGGACGGCATGGATT
TGTGGTGGCGATAACGAGTGTGGAAAATATCGGGAAGGGCCTGATTCGGG
ATGGGACAGGGTTCGTGACTTTTCCAGTGAAGTACCAGTGTGTTGTTTTC
AGACCCTTTAAAGGGGAAATTTTGGAAGCTGTTGTTTCCATGGTTAACAA
GATGGGGTTTTTCGCTGAAGCTGGACCTGTCCAAATTTTTGTTTCAAATC
ATTTGATACCGGATGATATGGAGTTCCA
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