CF509642 | Citrus Genome Database

Overview


Name	CF509642
Unique Name	CF509642
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	433

Libraries


Library Name	Type
Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig10898	contig	Csv1_Contig10898:644..1071.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_POPTR (NADP-dependent malic enzyme OS=Populus trichocarpa PE=2 SV=3)

HSP 1 Score: 101.293 bits (251), Expect = 6.671e-46
Identity = 48/53 (90.57%), Postives = 52/53 (98.11%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            +T+DVGTNNEQLLKDEFYIGLRQRRATGQEY+ELL EFM+AVKQNYGEKVLIQ
Sbjct:  228 VTIDVGTNNEQLLKDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQ 280

HSP 2 Score: 62.7734 bits (151), Expect = 6.671e-46
Identity = 29/38 (76.32%), Postives = 36/38 (94.74%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEET 412
            +LA+H FLFLGAGEAGTGIAELIALE+S+++K P+EET
Sbjct:  328 SLADHTFLFLGAGEAGTGIAELIALEMSRRSKTPLEET 365

HSP 3 Score: 61.6178 bits (148), Expect = 6.671e-46
Identity = 27/28 (96.43%), Postives = 28/28 (100.00%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF+LLAKYGTTHLVFNDDIQ
Sbjct:  281 FEDFANHNAFDLLAKYGTTHLVFNDDIQ 308

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOC_FLAPR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei GN=MODA PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 7.092e-45
Identity = 45/53 (84.91%), Postives = 52/53 (98.11%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGTNNE++L DEFYIGLRQRRA+G+EYAEL++EFMSAVKQNYGEKVLIQ
Sbjct:  284 ITIDVGTNNEKMLNDEFYIGLRQRRASGKEYAELMNEFMSAVKQNYGEKVLIQ 336

HSP 2 Score: 68.9366 bits (167), Expect = 7.092e-45
Identity = 33/38 (86.84%), Postives = 37/38 (97.37%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEET 412
            +LA+H+FLFLGAGEAGTGIAELIALEISKQT AP+EET
Sbjct:  384 SLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEET 421

HSP 3 Score: 56.6102 bits (135), Expect = 7.092e-45
Identity = 25/28 (89.29%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF+LL KY TTHLVFNDDIQ
Sbjct:  337 FEDFANHNAFDLLEKYRTTHLVFNDDIQ 364

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_VITVI (NADP-dependent malic enzyme OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 9.240e-45
Identity = 43/53 (81.13%), Postives = 49/53 (92.45%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGTNNE+LL +EFYIGL+QRRATG+EY+E L EFMS VKQNYGEKVLIQ
Sbjct:  228 ITIDVGTNNEKLLANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQ 280

HSP 2 Score: 70.4774 bits (171), Expect = 9.240e-45
Identity = 33/38 (86.84%), Postives = 37/38 (97.37%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEET 412
            TLA+H+FLFLGAGEAGTGIAELIALE+SKQTK P+EET
Sbjct:  328 TLADHKFLFLGAGEAGTGIAELIALEMSKQTKCPIEET 365

HSP 3 Score: 60.077 bits (144), Expect = 9.240e-45
Identity = 26/28 (92.86%), Postives = 27/28 (96.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF+LLAKYGTTHL FNDDIQ
Sbjct:  281 FEDFANHNAFDLLAKYGTTHLAFNDDIQ 308

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_PHAVU (NADP-dependent malic enzyme OS=Phaseolus vulgaris GN=ME1 PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 9.241e-45
Identity = 45/53 (84.91%), Postives = 49/53 (92.45%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            +T+DVGTNNE+LL DEFYIGLRQRRATGQEYA  LDEFM AVKQNYGEKVL+Q
Sbjct:  226 VTIDVGTNNEKLLNDEFYIGLRQRRATGQEYATFLDEFMRAVKQNYGEKVLVQ 278

HSP 2 Score: 70.8626 bits (172), Expect = 9.241e-45
Identity = 34/38 (89.47%), Postives = 37/38 (97.37%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEET 412
            TLA+H FLFLGAGEAGTGIAELIA+E+SKQTKAPVEET
Sbjct:  326 TLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVEET 363

HSP 3 Score: 54.299 bits (129), Expect = 9.241e-45
Identity = 23/28 (82.14%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF+LL KY ++HLVFNDDIQ
Sbjct:  279 FEDFANHNAFDLLEKYSSSHLVFNDDIQ 306

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOC_ORYSJ (NADP-dependent malic enzyme, chloroplastic OS=Oryza sativa subsp. japonica GN=ME6 PE=2 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 2.029e-44
Identity = 46/53 (86.79%), Postives = 50/53 (94.34%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGTNNEQLL DEFYIGLRQRRATG+EY EL++EFMSAVKQ YGEKVLIQ
Sbjct:  276 ITIDVGTNNEQLLNDEFYIGLRQRRATGKEYHELMEEFMSAVKQIYGEKVLIQ 328

HSP 2 Score: 70.0922 bits (170), Expect = 2.029e-44
Identity = 34/37 (91.89%), Postives = 36/37 (97.30%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEE 409
            TLAEH +LFLGAGEAGTGIAELIALEISKQTKAP+EE
Sbjct:  376 TLAEHTYLFLGAGEAGTGIAELIALEISKQTKAPIEE 412

HSP 3 Score: 55.4546 bits (132), Expect = 2.029e-44
Identity = 24/28 (85.71%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF+LLAKY  +HLVFNDDIQ
Sbjct:  329 FEDFANHNAFDLLAKYSKSHLVFNDDIQ 356

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_MESCR (NADP-dependent malic enzyme OS=Mesembryanthemum crystallinum GN=MOD1 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.163e-43
Identity = 41/53 (77.36%), Postives = 50/53 (94.34%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGTNN++LL DEFYIGL+Q+RATG+EYAE + EFMSAVKQNYGEK+L+Q
Sbjct:  222 ITLDVGTNNQKLLDDEFYIGLKQKRATGEEYAEFVQEFMSAVKQNYGEKILVQ 274

HSP 2 Score: 67.781 bits (164), Expect = 2.163e-43
Identity = 32/37 (86.49%), Postives = 37/37 (100.00%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEE 409
            TLA+H+FLFLGAGEAGTGIAELIALE+SK+TKAPVE+
Sbjct:  322 TLADHKFLFLGAGEAGTGIAELIALEMSKKTKAPVEQ 358

HSP 3 Score: 57.7658 bits (138), Expect = 2.163e-43
Identity = 26/28 (92.86%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAFELL KY TTHLVFNDDIQ
Sbjct:  275 FEDFANHNAFELLEKYRTTHLVFNDDIQ 302

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOC_MAIZE (NADP-dependent malic enzyme, chloroplastic OS=Zea mays GN=MOD1 PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.170e-41
Identity = 44/53 (83.02%), Postives = 49/53 (92.45%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGTNNE LL DEFYIGLRQ+RATG+EY EL++EFMSAVKQ YGEKVLIQ
Sbjct:  273 ITIDVGTNNEFLLNDEFYIGLRQKRATGEEYDELIEEFMSAVKQFYGEKVLIQ 325

HSP 2 Score: 65.0846 bits (157), Expect = 3.170e-41
Identity = 32/37 (86.49%), Postives = 34/37 (91.89%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEE 409
            TLAE  +LFLGAGEAGTGIAELIALEISKQT AP+EE
Sbjct:  373 TLAEQTYLFLGAGEAGTGIAELIALEISKQTNAPIEE 409

HSP 3 Score: 53.5286 bits (127), Expect = 3.170e-41
Identity = 23/28 (82.14%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF+LL KY  +HLVFNDDIQ
Sbjct:  326 FEDFANHNAFDLLEKYSKSHLVFNDDIQ 353

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOC_FLATR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria trinervia GN=MOD1 PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.530e-40
Identity = 43/54 (79.63%), Postives = 50/54 (92.59%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKD-EFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            IT+DVGTNNE+LL D EFYIGL+Q+RA GQEYAEL++EFMSAVKQNYGE +LIQ
Sbjct:  284 ITIDVGTNNEKLLNDDEFYIGLKQKRAAGQEYAELMNEFMSAVKQNYGENLLIQ 337

HSP 2 Score: 62.3882 bits (150), Expect = 1.530e-40
Identity = 30/38 (78.95%), Postives = 35/38 (92.11%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEET 412
            +LA+ +FLFLGAGEAGTGIAELIALEISKQT  P+EE+
Sbjct:  385 SLADQKFLFLGAGEAGTGIAELIALEISKQTNIPLEES 422

HSP 3 Score: 56.6102 bits (135), Expect = 1.530e-40
Identity = 25/28 (89.29%), Postives = 26/28 (92.86%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF+LL KY TTHLVFNDDIQ
Sbjct:  338 FEDFANHNAFDLLEKYRTTHLVFNDDIQ 365

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOC_SOLLC (NADP-dependent malic enzyme, chloroplastic (Fragment) OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 5.698e-40
Identity = 41/53 (77.36%), Postives = 46/53 (86.79%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            +T+DVG    + + DEFYIGLRQRRATGQEY+ELLDEFM AVKQNYGEKVLIQ
Sbjct:  212 VTIDVG-QTMKFVDDEFYIGLRQRRATGQEYSELLDEFMYAVKQNYGEKVLIQ 263

HSP 2 Score: 63.929 bits (154), Expect = 5.698e-40
Identity = 31/38 (81.58%), Postives = 35/38 (92.11%), Query Frame = 2

Query:  299 TLAEHRFLFLGAGEAGTGIAELIALEISKQTKAPVEET 412
            +L+EH FLFLGAGEAGTGIAELIALE+SKQT  P+EET
Sbjct:  311 SLSEHTFLFLGAGEAGTGIAELIALEMSKQTGIPLEET 348

HSP 3 Score: 59.3066 bits (142), Expect = 5.698e-40
Identity = 26/28 (92.86%), Postives = 27/28 (96.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFANHNAF LLAKYGT+HLVFNDDIQ
Sbjct:  264 FEDFANHNAFNLLAKYGTSHLVFNDDIQ 291

[Close]

BLAST of CF509642 vs. ExPASy Swiss-Prot
Match: MAOX_HUMAN (NADP-dependent malic enzyme OS=Homo sapiens GN=ME1 PE=1 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.765e-21
Identity = 33/53 (62.26%), Postives = 37/53 (69.81%), Query Frame = 1

Query:    1 ITVDVGTNNEQLLKDEFYIGLRQRRATGQEYAELLDEFMSAVKQNYGEKVLIQ 159
            + +DVGT NE+LLKD  YIGLRQRR  G EY + LDEFM AV   YG   LIQ
Sbjct:  191 VILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQ 243

HSP 2 Score: 41.2022 bits (95), Expect = 1.765e-21
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  165 FEDFANHNAFELLAKYGTTHLVFNDDIQ 248
            FEDFAN NAF LL KY   +  FNDDIQ
Sbjct:  244 FEDFANVNAFRLLNKYRNQYCTFNDDIQ 271

HSP 3 Score: 31.5722 bits (70), Expect = 1.765e-21
Identity = 14/29 (48.28%), Postives = 19/29 (65.52%), Query Frame = 2

Query:  302 LAEHRFLFLGAGEAGTGIAELIALEISKQ 388
            L++   LF GAGEA  GIA LI + + K+
Sbjct:  292 LSDQTILFQGAGEAALGIAHLIVMALEKE 320

The following BLAST results are available for this feature:

BLAST of CF509642 vs. ExPASy Swiss-Prot

BLAST of CF509642 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 133


Match Name	E-value	Identity	Description
MAOX_POPTR	6.671e-46	90.57	NADP-dependent malic enzyme OS=Populus trichocarpa...	[more]
MAOC_FLAPR	7.092e-45	84.91	NADP-dependent malic enzyme, chloroplastic OS=Flav...	[more]
MAOX_VITVI	9.240e-45	81.13	NADP-dependent malic enzyme OS=Vitis vinifera PE=2...	[more]
MAOX_PHAVU	9.241e-45	84.91	NADP-dependent malic enzyme OS=Phaseolus vulgaris ...	[more]
MAOC_ORYSJ	2.029e-44	86.79	NADP-dependent malic enzyme, chloroplastic OS=Oryz...	[more]
MAOX_MESCR	2.163e-43	77.36	NADP-dependent malic enzyme OS=Mesembryanthemum cr...	[more]
MAOC_MAIZE	3.170e-41	83.02	NADP-dependent malic enzyme, chloroplastic OS=Zea ...	[more]
MAOC_FLATR	1.530e-40	79.63	NADP-dependent malic enzyme, chloroplastic OS=Flav...	[more]
MAOC_SOLLC	5.698e-40	77.36	NADP-dependent malic enzyme, chloroplastic (Fragme...	[more]
MAOX_HUMAN	1.765e-21	62.26	NADP-dependent malic enzyme OS=Homo sapiens GN=ME1...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_125000-150 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-7_F07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF509642 ID=CF509642; Name=CF509642; organism=Citrus sinensis; type=EST; length=433bp
ATAACCGTTGATGTGGGTACAAACAATGAGCAATTGTTGAAGGATGAATT
CTACATTGGTCTACGACAGAGGAGGGCAACCGGACAGGAATATGCTGAAC
TTCTAGATGAATTCATGAGTGCTGTCAAGCAAAATTATGGAGAAAAAGTT
CTTATACAGGTTAGTTTGAGGATTTCGCAAACCACAATGCTTTTGAGTTG
CTTGCAAAATATGGCACAACTCATCTTGTCTTCAATGATGATATACAGCG
TCTGTTGTTCTTGCTGGGGTAGTTGCAGCACTGAAGTTGATTGGTGGCAC
ACTGGCCGAGCATAGATTTTTGTTTCTTGGAGCTGGGGAGGCTGGGACTG
GTATAGCTGAACTTATTGCGCTTGAGATATCAAAGCAGACTAAAGCTCCT
GTGGAAGAGACCCCGGAAGAAGATATGTCTTGT