CF832574

Overview
NameCF832574
Unique NameCF832574
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length716
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9752 contig Csv1_Contig9752:92..807. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_ARATH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Arabidopsis thaliana GN=CIMS PE=1 SV=1)

HSP 1 Score: 464.922 bits (1195), Expect = 1.870e-130
Identity = 224/238 (94.12%), Postives = 232/238 (97.48%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPP+IYGDVSRPKAMTVFWS++AQSMT+RPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEK GI VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQD+TQIHTHMCYS+FNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS+EEIADR+NKMLAVLE NILWVNPDCGLKTRKYTEVKPAL NMV AAK
Sbjct:  518 YGSRCVKPPVIYGDVSRPKAMTVFWSAMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLAVLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAK 755
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_SOLSC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Solenostemon scutellarioides GN=MET PE=1 SV=2)

HSP 1 Score: 461.84 bits (1187), Expect = 1.583e-129
Identity = 227/238 (95.38%), Postives = 229/238 (96.22%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDVSRPK MTVFWS+ AQSMT RPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEKAGI VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSII+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKY EVKPAL NMVAAAK
Sbjct:  517 YGSRCVKPPIIYGDVSRPKPMTVFWSTAAQSMTQRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYAEVKPALENMVAAAK 754
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_MESCR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum GN=METE PE=2 SV=1)

HSP 1 Score: 456.833 bits (1174), Expect = 5.093e-128
Identity = 223/238 (93.70%), Postives = 228/238 (95.80%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDVSRP  MTVFWSS+AQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIK+EVEDLEKAGINVIQIDEAALREGLPLRKSEH FYL WAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIP TEE+ADRI KMLAVLE+N+LWVNPDCGLKTRKY EV PALSNMVAAAK
Sbjct:  518 YGSRCVKPPIIYGDVSRPNPMTVFWSSMAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKNEVEDLEKAGINVIQIDEAALREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPPTEELADRIRKMLAVLESNVLWVNPDCGLKTRKYGEVNPALSNMVAAAK 755
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_CATRO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus GN=METE PE=2 SV=1)

HSP 1 Score: 452.595 bits (1163), Expect = 9.604e-127
Identity = 221/238 (92.86%), Postives = 226/238 (94.96%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPIIYGDVSRP  MTVFWS  AQSMT RPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRK+EHAFYLDWAVHSFRITN  +QDTTQIHTHMCYSNFNDIIHSIIDMDADV+TIENSRS EKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVL+TNILWVNPDCGLKTRKY EVKPAL NMV+AAK
Sbjct:  518 YGSRCVKPPIIYGDVSRPNPMTVFWSQTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSIIDMDADVMTIENSRSSEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLDTNILWVNPDCGLKTRKYAEVKPALENMVSAAK 755
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURS3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia sp. (strain 383) GN=metE PE=3 SV=1)

HSP 1 Score: 336.265 bits (861), Expect = 1.003e-91
Identity = 168/238 (70.59%), Postives = 191/238 (80.25%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++   YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ E I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DNFKYPNEIGPGVYDIHSPNIPTQEHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAK 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain HI2424) GN=metE PE=3 SV=1)

HSP 1 Score: 334.724 bits (857), Expect = 2.919e-91
Identity = 167/238 (70.17%), Postives = 192/238 (80.67%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWIAYAQSLTRKPMKGMLTGPVTILNWSFVRDDQPRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAK 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURCC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain MC0-3) GN=metE PE=3 SV=1)

HSP 1 Score: 334.724 bits (857), Expect = 2.919e-91
Identity = 167/238 (70.17%), Postives = 192/238 (80.67%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWIAYAQSLTRKPMKGMLTGPVTILNWSFVRDDQPRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMRKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAK 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURCA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain AU 1054) GN=metE PE=3 SV=1)

HSP 1 Score: 334.724 bits (857), Expect = 2.919e-91
Identity = 167/238 (70.17%), Postives = 192/238 (80.67%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI++EV DLEKAG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWIAYAQSLTRKPMKGMLTGPVTILNWSFVRDDQPRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DTFKYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAK 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURCM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=metE PE=3 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 3.812e-91
Identity = 166/238 (69.75%), Postives = 191/238 (80.25%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI+DEV DLEKAG+ VIQIDEAALREGLPLR+++   YL WAV +FRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  +Y   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLRRAQWGEYLKWAVEAFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DSFRYPNEIGPGVYDIHSPNIPTQDHIVGLMKKAAERIPAERLWVNPDCGLKTRQWAEVIPALTNMVAAAK 757
[Close]
BLAST of CF832574 vs. ExPASy Swiss-Prot
Match: METE_BURCJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metE PE=3 SV=1)

HSP 1 Score: 334.339 bits (856), Expect = 3.812e-91
Identity = 167/238 (70.17%), Postives = 192/238 (80.67%), Query Frame = 1 
Query:    1 YGSPCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLETNILWVNPDCGLKTRKYTEVKPALSNMVAAAK 714
            YGS CVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILNWSFVR+DQPR  +CYQ+ALAI+DEV DLE+AG+ VIQIDEAALREGLPLR+++ + YL WAV SFRIT  GVQD TQIHTHMCYS FNDII SI DMDADVITIE SRSD +LL  F +  KY   IGPGVYDIHSP IP+ + I   + K    +    LWVNPDCGLKTR++ EV PAL+NMVAAAK
Sbjct:  521 YGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEQAGVRVIQIDEAALREGLPLRRAQWSEYLKWAVESFRITANGVQDDTQIHTHMCYSEFNDIIASIADMDADVITIETSRSDMELLDAF-DDFKYPNEIGPGVYDIHSPNIPTQDHIVGLMRKAAERIPAERLWVNPDCGLKTRQWGEVIPALTNMVAAAK 757
The following BLAST results are available for this feature:
BLAST of CF832574 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 286
Match NameE-valueIdentityDescription
METE_ARATH1.870e-13094.125-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SOLSC1.583e-12995.385-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MESCR5.093e-12893.705-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CATRO9.604e-12792.865-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURS31.003e-9170.595-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCH2.919e-9170.175-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCC2.919e-9170.175-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCA2.919e-9170.175-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCM3.812e-9169.755-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCJ3.812e-9170.175-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02B06_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02B06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832574 ID=CF832574; Name=CF832574; organism=Citrus sinensis; type=EST; length=716bp
TATGGATCTCCCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCG
TCCCAAGGCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACAG
CTCGCCCAATGAAAGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGG
TCCTTTGTTAGAAATGACCAGCCCAGACATGAAACCTGCTACCAGATTGC
TTTGGCCATCAAGGACGAAGTCGAGGATCTTGAGAAGGCAGGCATTAACG
TTATTCAAATTGATGAGGCTGCCTTGAGGGAAGGATTGCCTCTTAGGAAA
TCTGAGCATGCTTTCTACTTGGACTGGGCAGTCCACTCCTTCAGAATCAC
CAACTGTGGCGTCCAGGACACTACCCAGATCCACACCCACATGTGCTACT
CCAACTTCAACGACATTATCCACTCAATTATCGACATGGATGCTGATGTG
ATCACCATTGAGAACTCTCGTTCTGATGAGAAGCTCCTGTCTGTCTTCCG
TGAGGGAGTCAAGTATGGTGCTGGAATTGGCCCTGGTGTGTATGACATCC
ACTCTCCAAGAATACCATCAACTGAAGAAATTGCTGACAGAATCAACAAG
ATGCTTGCTGTGCTAGAAACCAACATTTTGTGGGTTAACCCTGACTGTGG
TCTTAAGACTCGCAAGTACACTGAGGTGAAGCCAGCCCTCTCAAACATGG
TCGCTGCTGCCAAGCT
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