CF833225

Overview
NameCF833225
Unique NameCF833225
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length181
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13335 contig Csv1_Contig13335:1116..1296. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF833225 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 5.638e-19
Identity = 44/50 (88.00%), Postives = 46/50 (92.00%), Query Frame = 2 
Query:    2 GSGAGTSRTVKWKGFRVITSATDAPAFTPGSFIAGSIWLGSTGFPFSLGL 151
            G+GAGTS  VKWKGFRVITSAT+A AFTPGSFIAGS WLGSTGFPFSLGL
Sbjct:  535 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
[Close]
BLAST of CF833225 vs. ExPASy Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 5.638e-19
Identity = 44/50 (88.00%), Postives = 46/50 (92.00%), Query Frame = 2 
Query:    2 GSGAGTSRTVKWKGFRVITSATDAPAFTPGSFIAGSIWLGSTGFPFSLGL 151
            G+GAGTS  VKWKGFRVITSAT+A AFTPGSFIAGS WLGSTGFPFSLGL
Sbjct:  535 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
[Close]
BLAST of CF833225 vs. ExPASy Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.877e-14
Identity = 36/50 (72.00%), Postives = 41/50 (82.00%), Query Frame = 2 
Query:    2 GSGAGTSRTVKWKGFRVITSATDAPAFTPGSFIAGSIWLGSTGFPFSLGL 151
            G+GA TS  VKWKG +VITS+T+A A+TPG FIAG  WL STGFPFSLGL
Sbjct:  534 GAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
[Close]
BLAST of CF833225 vs. ExPASy Swiss-Prot
Match: PME_DAUCA (Pectinesterase OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 9.315e-14
Identity = 35/50 (70.00%), Postives = 40/50 (80.00%), Query Frame = 2 
Query:    2 GSGAGTSRTVKWKGFRVITSATDAPAFTPGSFIAGSIWLGSTGFPFSLGL 151
            G+GA TS  V WKGF+VITS+T+A  FTPGSFIAG  WL +T FPFSLGL
Sbjct:  270 GAGAATSGRVTWKGFKVITSSTEAQGFTPGSFIAGGSWLKATTFPFSLGL 319
[Close]
BLAST of CF833225 vs. ExPASy Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 4.623e-13
Identity = 32/50 (64.00%), Postives = 39/50 (78.00%), Query Frame = 2 
Query:    2 GSGAGTSRTVKWKGFRVITSATDAPAFTPGSFIAGSIWLGSTGFPFSLGL 151
            G+GAGT+  VKW+GF+VIT+A +A  +T G FI G  WL STGFPFSLGL
Sbjct:  543 GAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
[Close]
BLAST of CF833225 vs. ExPASy Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 7.886e-13
Identity = 33/50 (66.00%), Postives = 37/50 (74.00%), Query Frame = 2 
Query:    2 GSGAGTSRTVKWKGFRVITSATDAPAFTPGSFIAGSIWLGSTGFPFSLGL 151
            G GAGT+  VKWKG++VITS T+A  FT G FI G  WL STGFPFSL L
Sbjct:  538 GGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
The following BLAST results are available for this feature:
BLAST of CF833225 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 6
Match NameE-valueIdentityDescription
PME3_CITSI5.638e-1988.00Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PME1_CITSI5.638e-1988.00Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE... [more]
PMEU1_SOLLC1.877e-1472.00Pectinesterase/pectinesterase inhibitor U1 OS=Sola... [more]
PME_DAUCA9.315e-1470.00Pectinesterase OS=Daucus carota PE=1 SV=1[more]
PME3_ARATH4.623e-1364.00Pectinesterase/pectinesterase inhibitor 3 OS=Arabi... [more]
PME2_ARATH7.886e-1366.00Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 P... [more]
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Properties
Property NameValue
Genbank descriptionUCRCS02_03D20_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa03D20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF833225 ID=CF833225; Name=CF833225; organism=Citrus sinensis; type=EST; length=181bp
CGGATCCGGTGCCGGAACTTCACGGACAGTGAAATGGAAGGGATTTAGGG
TTATTACGAGTGCTACCGACGCTCCAGCTTTTACTCCTGGAAGCTTCATT
GCTGGTAGTATCTGGCTGGGCTCCACTGGTTTCCCATTCTCCCTTGGTTT
GTAATATTCACTAGGAGTTTTAATTACTATG
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