CF834231

Overview
NameCF834231
Unique NameCF834231
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length672
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1001 contig Csv1_Contig1001:644..1315. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_BETVU (Elongation factor 2 OS=Beta vulgaris PE=2 SV=1)

HSP 1 Score: 370.933 bits (951), Expect = 3.304e-102
Identity = 180/197 (91.37%), Postives = 188/197 (95.43%), Query Frame = 1 
Query:    1 VFDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            V DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLH DAIHRGGGQ+IPTARRV YASQLTAKPRLLEPVY+VEIQAPE ALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPV+ESFGFS TLRA+TSGQAFPQCVFDHW+MM SDPLE G+QA+ LV+ IRKRKGLKEQMTPLSEFEDKL
Sbjct:  647 VVDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHTDAIHRGGGQIIPTARRVFYASQLTAKPRLLEPVYLVEIQAPENALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFSSTLRASTSGQAFPQCVFDHWEMMPSDPLEAGSQASTLVSVIRKRKGLKEQMTPLSEFEDKL 843
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_CHLKE (Elongation factor 2 OS=Chlorella kessleri PE=2 SV=1)

HSP 1 Score: 328.561 bits (841), Expect = 1.880e-89
Identity = 158/197 (80.20%), Postives = 177/197 (89.85%), Query Frame = 1 
Query:    1 VFDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            V D+ KGVQYLNEIKDSVVA FQWASKEG LAEENMRGI FEVCDVVLHADAIHRGGGQ+IPTARR +YA+QLTA+PRLLEPVY+VEIQ PEQA+GG+YSVLNQKRG VFEE+QRPGTP++N+KAYLPVIESFGF+ TLRAAT+GQAFPQCVFDHW+ M SDP + G+QA  LV DIRKRKGLK +   LSE+EDKL
Sbjct:  649 VTDITKGVQYLNEIKDSVVAAFQWASKEGVLAEENMRGIVFEVCDVVLHADAIHRGGGQIIPTARRSMYAAQLTAQPRLLEPVYLVEIQCPEQAMGGVYSVLNQKRGMVFEELQRPGTPIFNLKAYLPVIESFGFTSTLRAATAGQAFPQCVFDHWEAMGSDPTQVGSQANTLVMDIRKRKGLKPEPAALSEYEDKL 845
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_CAEEL (Elongation factor 2 OS=Caenorhabditis elegans GN=eft-2 PE=2 SV=4)

HSP 1 Score: 287.345 bits (734), Expect = 4.804e-77
Identity = 137/197 (69.54%), Postives = 162/197 (82.23%), Query Frame = 1 
Query:    1 VFDMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            + D+ KGVQYLNEIKDSVVAGFQWA++EG L++ENMRG+ F V DV LHADAIHRGGGQ+IPTARRV YAS LTA+PRLLEPVY+VEIQ PE A+GGIY VLN++RGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DPLE GT+  Q+V D RKRKGLKE +  L  + DK+
Sbjct:  656 LMDVTKGVQYLNEIKDSVVAGFQWATREGVLSDENMRGVRFNVHDVTLHADAIHRGGGQIIPTARRVFYASVLTAEPRLLEPVYLVEIQCPEAAVGGIYGVLNRRRGHVFEESQVTGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPLEAGTKPNQIVLDTRKRKGLKEGVPALDNYLDKM 852
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_RAT (Elongation factor 2 OS=Rattus norvegicus GN=Eef2 PE=1 SV=4)

HSP 1 Score: 286.96 bits (733), Expect = 6.274e-77
Identity = 136/195 (69.74%), Postives = 162/195 (83.08%), Query Frame = 1 
Query:    7 DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  664 DITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_PONAB (Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 SV=3)

HSP 1 Score: 286.96 bits (733), Expect = 6.274e-77
Identity = 136/195 (69.74%), Postives = 162/195 (83.08%), Query Frame = 1 
Query:    7 DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  664 DITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_MOUSE (Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 SV=2)

HSP 1 Score: 286.96 bits (733), Expect = 6.274e-77
Identity = 136/195 (69.74%), Postives = 162/195 (83.08%), Query Frame = 1 
Query:    7 DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  664 DITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_MESAU (Elongation factor 2 OS=Mesocricetus auratus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 286.96 bits (733), Expect = 6.274e-77
Identity = 136/195 (69.74%), Postives = 162/195 (83.08%), Query Frame = 1 
Query:    7 DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  664 DITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_HUMAN (Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4)

HSP 1 Score: 286.96 bits (733), Expect = 6.274e-77
Identity = 136/195 (69.74%), Postives = 162/195 (83.08%), Query Frame = 1 
Query:    7 DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  664 DITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_CALJA (Elongation factor 2 OS=Callithrix jacchus GN=EEF2 PE=2 SV=1)

HSP 1 Score: 286.189 bits (731), Expect = 1.070e-76
Identity = 136/195 (69.74%), Postives = 162/195 (83.08%), Query Frame = 1 
Query:    7 DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  664 DITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
[Close]
BLAST of CF834231 vs. ExPASy Swiss-Prot
Match: EF2_BOVIN (Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 286.189 bits (731), Expect = 1.070e-76
Identity = 136/195 (69.74%), Postives = 162/195 (83.08%), Query Frame = 1 
Query:    7 DMCKGVQYLNEIKDSVVAGFQWASKEGALAEENMRGICFEVCDVVLHADAIHRGGGQVIPTARRVIYASQLTAKPRLLEPVYMVEIQAPEQALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 591
            D+ KGVQYLNEIKDSVVAGFQWA+KEGAL EENMRG+ F+V DV LHADAIHRGGGQ+IPTARR +YAS LTA+PRL+EP+Y+VEIQ PEQ +GGIY VLN+KRGHVFEE Q  GTP++ +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  664 DITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEETQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858
The following BLAST results are available for this feature:
BLAST of CF834231 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 125
Match NameE-valueIdentityDescription
EF2_BETVU3.304e-10291.37Elongation factor 2 OS=Beta vulgaris PE=2 SV=1[more]
EF2_CHLKE1.880e-8980.20Elongation factor 2 OS=Chlorella kessleri PE=2 SV=... [more]
EF2_CAEEL4.804e-7769.54Elongation factor 2 OS=Caenorhabditis elegans GN=e... [more]
EF2_RAT6.274e-7769.74Elongation factor 2 OS=Rattus norvegicus GN=Eef2 P... [more]
EF2_PONAB6.274e-7769.74Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 S... [more]
EF2_MOUSE6.274e-7769.74Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 S... [more]
EF2_MESAU6.274e-7769.74Elongation factor 2 OS=Mesocricetus auratus GN=EEF... [more]
EF2_HUMAN6.274e-7769.74Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 S... [more]
EF2_CALJA1.070e-7669.74Elongation factor 2 OS=Callithrix jacchus GN=EEF2 ... [more]
EF2_BOVIN1.070e-7669.74Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05C14_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05C14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834231 ID=CF834231; Name=CF834231; organism=Citrus sinensis; type=EST; length=672bp
GTCTTTGATATGTGTAAGGGAGTTCAATACTTGAATGAAATTAAGGATTC
TGTTGTTGCTGGTTTCCAGTGGGCATCGAAAGAAGGTGCGCTGGCTGAAG
AGAACATGAGAGGTATCTGCTTTGAAGTATGTGATGTTGTTCTTCACGCT
GATGCCATTCACAGAGGTGGTGGTCAAGTCATTCCTACTGCTAGGAGGGT
CATATATGCTTCCCAGCTCACAGCCAAGCCCAGGCTTCTTGAGCCTGTCT
ACATGGTCGAAATACAGGCTCCCGAGCAGGCCCTTGGTGGTATTTACAGT
GTTCTCAATCAGAAACGTGGTCACGTGTTCGAGGAAATGCAGAGACCTGG
TACCCCCCTTTACAACATCAAGGCTTACCTTCCCGTCATCGAGTCCTTTG
GATTCTCAGGCACTTTGAGGGCTGCAACGTCAGGACAGGCCTTCCCACAA
TGTGTGTTTGATCACTGGGACATGATGTCATCCGATCCACTGGAGCCTGG
TACACAGGCTGCACAGCTTGTTGCTGATATCAGGAAGAGGAAGGGCTTGA
AGGAGCAAATGACACCACTATCTGAGTTTGAGGACAAGCTGTAAACATTT
CGTCTTGACATCAAGGGTGAGATTGCGCAGCTTGTTGCTGATATCAGGAA
GAGGGAGCAAATGACACCGCTA
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