CF835868

Overview
NameCF835868
Unique NameCF835868
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length488
Libraries
Library NameType
Washington Navel Orange Shoot Meristem cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHC2_ARATH (Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis thaliana GN=At5g43330 PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.283e-30
Identity = 61/74 (82.43%), Postives = 65/74 (87.84%), Query Frame = -3
Query:  265 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYSC 486
            DWV GTPEGT+VSMGVY DGSYNVPAG+IYSFPVTCRNGEWTIVQG  ID+  RKK+DLTAEE  EEK LAYSC
Sbjct:  257 DWVVGTPEGTFVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLPIDDASRKKMDLTAEELKEEKDLAYSC 330          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHC_MESCR (Malate dehydrogenase, cytoplasmic OS=Mesembryanthemum crystallinum GN=MDH PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 2.188e-30
Identity = 59/74 (79.73%), Postives = 63/74 (85.14%), Query Frame = -3
Query:  265 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYSC 486
            DWV GTPEGTWVSMGVY DGSYNVPAG+IYSFPVTC+NGEWTIVQG  ID+  RKK+D TA E  EEK LAYSC
Sbjct:  257 DWVLGTPEGTWVSMGVYSDGSYNVPAGIIYSFPVTCKNGEWTIVQGLPIDDDSRKKMDATAAELVEEKTLAYSC 330          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHC_MEDSA (Malate dehydrogenase, cytoplasmic OS=Medicago sativa GN=CMDH PE=2 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 2.858e-30
Identity = 60/74 (81.08%), Postives = 66/74 (89.19%), Query Frame = -3
Query:  265 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYSC 486
            DWV GTP+GT+VSMGVY DGSYNVP+G+IYSFPVTC NGEW IVQG SIDEF RKKLDLTAEE +EEK LA+SC
Sbjct:  257 DWVLGTPQGTFVSMGVYSDGSYNVPSGLIYSFPVTCANGEWKIVQGLSIDEFSRKKLDLTAEELTEEKNLAHSC 330          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHC_ORYSJ (Malate dehydrogenase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os10g0478200 PE=1 SV=3)

HSP 1 Score: 129.028 bits (323), Expect = 1.086e-29
Identity = 59/74 (79.73%), Postives = 63/74 (85.14%), Query Frame = -3
Query:  265 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYSC 486
            DWV GTPEGT+VSMGVY DGSY VPAG+IYSFPVTC  GEWTIVQG  IDEF RKK+D TA+E SEEK LAYSC
Sbjct:  257 DWVLGTPEGTFVSMGVYSDGSYGVPAGLIYSFPVTCSGGEWTIVQGLPIDEFSRKKMDATAQELSEEKTLAYSC 330          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHC1_ARATH (Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis thaliana GN=At1g04410 PE=1 SV=2)

HSP 1 Score: 128.257 bits (321), Expect = 1.852e-29
Identity = 58/74 (78.38%), Postives = 65/74 (87.84%), Query Frame = -3
Query:  265 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYSC 486
            DWV GTPEGT+VSMGVY DGSY+VP+G+IYSFPVTCRNG+W+IVQG  IDE  RKK+DLTAEE  EEK LAYSC
Sbjct:  257 DWVLGTPEGTFVSMGVYSDGSYSVPSGLIYSFPVTCRNGDWSIVQGLPIDEVSRKKMDLTAEELKEEKDLAYSC 330          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHC_BETVU (Malate dehydrogenase, cytoplasmic OS=Beta vulgaris GN=NR1 PE=1 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 3.160e-29
Identity = 57/74 (77.03%), Postives = 62/74 (83.78%), Query Frame = -3
Query:  265 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYSC 486
            DWV GTPEGTWVSMGVY DGSYNVPAG+IYSFPVTC++GEW IVQG  IDE  R+K+D T  E  EEKALAYSC
Sbjct:  257 DWVLGTPEGTWVSMGVYSDGSYNVPAGIIYSFPVTCKDGEWKIVQGLPIDEVSRQKMDATGAELVEEKALAYSC 330          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHC_MAIZE (Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2 SV=2)

HSP 1 Score: 124.405 bits (311), Expect = 2.675e-28
Identity = 56/74 (75.68%), Postives = 62/74 (83.78%), Query Frame = -3
Query:  265 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYSC 486
            DWV GTPEGT+VSMGVY DGSY VP+G+IYSFPVTC  GEW IVQG  IDEF RKK+D TA+E +EEK LAYSC
Sbjct:  257 DWVLGTPEGTFVSMGVYSDGSYGVPSGLIYSFPVTCSGGEWKIVQGLPIDEFSRKKMDATAQELTEEKTLAYSC 330          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDH_SACD2 (Malate dehydrogenase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=mdh PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 4.573e-20
Identity = 41/68 (60.29%), Postives = 48/68 (70.59%), Query Frame = -3
Query:  283 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEK 486
            DW  GTPE  WVSMGVY DGSY +  G+IYSFP  C+NG+W IVQG +IDEF R K+  T  E  EE+
Sbjct:  251 DWALGTPENDWVSMGVYSDGSYGIEEGLIYSFPCVCKNGDWEIVQGLTIDEFSRAKMTATENELKEER 318          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDHA_DICDI (Probable malate dehydrogenase 1 OS=Dictyostelium discoideum GN=mdhA PE=3 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 7.801e-20
Identity = 43/73 (58.90%), Postives = 53/73 (72.60%), Query Frame = -3
Query:  268 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEKALAYS 486
            DW+ GT +G  VSM VY DGSYNVP G+I+SFPVTC NG+WTIVQG  I+   ++K+D T +E  EEK  A S
Sbjct:  316 DWLLGTKDGEHVSMAVYSDGSYNVPKGLIFSFPVTCHNGQWTIVQGLKINSSTQQKIDATIKELQEEKETAMS 388          
BLAST of CF835868 vs. ExPASy Swiss-Prot
Match: MDH_DEIGD (Malate dehydrogenase OS=Deinococcus geothermalis (strain DSM 11300) GN=mdh PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.019e-19
Identity = 41/68 (60.29%), Postives = 50/68 (73.53%), Query Frame = -3
Query:  283 DWVFGTPEGTWVSMGVYFDGSYNVPAGVIYSFPVTCRNGEWTIVQGFSIDEFLRKKLDLTAEEFSEEK 486
            DW  GTPEG WVSM V  DGSY +P G+IY FPV CRNG++ IVQG  I +F R+K+D TA+E  EE+
Sbjct:  257 DWALGTPEGEWVSMAVPSDGSYGIPEGLIYGFPVRCRNGQYEIVQGLEISDFSRQKMDATAKELEEER 324          
The following BLAST results are available for this feature:
BLAST of CF835868 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 139
Match NameE-valueIdentityDescription
MDHC2_ARATH1.283e-3082.43Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis... [more]
MDHC_MESCR2.188e-3079.73Malate dehydrogenase, cytoplasmic OS=Mesembryanthe... [more]
MDHC_MEDSA2.858e-3081.08Malate dehydrogenase, cytoplasmic OS=Medicago sati... [more]
MDHC_ORYSJ1.086e-2979.73Malate dehydrogenase, cytoplasmic OS=Oryza sativa ... [more]
MDHC1_ARATH1.852e-2978.38Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis... [more]
MDHC_BETVU3.160e-2977.03Malate dehydrogenase, cytoplasmic OS=Beta vulgaris... [more]
MDHC_MAIZE2.675e-2875.68Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2... [more]
MDH_SACD24.573e-2060.29Malate dehydrogenase OS=Saccharophagus degradans (... [more]
MDHA_DICDI7.801e-2058.90Probable malate dehydrogenase 1 OS=Dictyostelium d... [more]
MDH_DEIGD1.019e-1960.29Malate dehydrogenase OS=Deinococcus geothermalis (... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS03_02A16_f Washington Navel Orange Shoot Meristem cDNA Library Citrus sinensis cDNA clone CS_PEa02A16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835868 ID=CF835868; Name=CF835868; organism=Citrus sinensis; type=EST; length=488bp
AAAAAAAAAATTGTTATTGATAGTTTGATACATCCTTCAAAAGGGGCCTT
TAATATCCATTTATCCAGGAAAAGAAACAAAACTCCAAAATCCCGATATT
CAAATGCAAAAATACCAACATAAAATGGAAAGGCTTCTCCATCATCAATG
GTTCCCCCAGGTTTCAACAATGGTTTTATTCAAAACTGTAACTATACCAA
CTACTCAAACAAAGCTAAGGGCCTTGGTAACCCCCTATTGAAGGGAAAAA
AGCATTTAAAAAAAACATGAGTATGCGAGGGCCTTCTCCTCGGAAAACTC
CTCTGCTGTCAAATCCAACTTCTTCCTTAAAAATTCATCAATGCTGAATC
CTTGAACAATTGTCCATTCCCCGTTGCGGCAGGTTACTGGGAATGAATAA
ATGACCCCAGCTGGTACATTGTATGAACCATCAAAATATACCCCCATGGA
AACCCAGGTGCCCTCAGGAGTTCCAAAAACCCAATCAC
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