CF836623

Overview
NameCF836623
Unique NameCF836623
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length845
Libraries
Library NameType
Washington Navel Orange Shoot Meristem cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13335 contig Csv1_Contig13335:602..1446. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 458.373 bits (1178), Expect = 2.309e-128
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL
Sbjct:  364 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 454.136 bits (1167), Expect = 4.355e-127
Identity = 218/221 (98.64%), Postives = 220/221 (99.55%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGR DPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITD+IHPAGWHEWDGNFALNTLFYGEHQN+GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL
Sbjct:  364 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 402.519 bits (1033), Expect = 1.504e-111
Identity = 192/221 (86.88%), Postives = 205/221 (92.76%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDITFQNTAG SKHQAVAL VG+DLSAFY CDMLAYQDTLYVHSNRQFFV CL+AGTVDFIFGN AAV Q+CDIHAR+P SGQKNMVTAQGRTDPNQNTGIVIQK RIGATSDL+PVQ SFPTYLGRPWKEYSRTVIMQSSITD+I PAGWHEW+GNFAL+TLFYGE+ N+GAGA TSGRVKWKG +VITS+TEAQA+TPG FIAG SWL STGFPFSLGL
Sbjct:  363 RDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME_DAUCA (Pectinesterase OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 399.053 bits (1024), Expect = 1.663e-110
Identity = 188/221 (85.07%), Postives = 207/221 (93.67%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDITFQNTAG +KHQAVALRVG+DLSAFY CD+LAYQD+LYVHSNRQFF+NC IAGTVDFIFGNAA VLQ+CDIHAR+P SGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDL+PVQ SFPTYLGRPWKEYSRTV+MQSSIT++I+PAGW  WDGNFAL+TL+YGE+QN+GAGA TSGRV WKGF+VITS+TEAQ FTPGSFIAG SWL +T FPFSLGL
Sbjct:   99 RDITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQFFINCFIAGTVDFIFGNAAVVLQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVQSSFPTYLGRPWKEYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAATSGRVTWKGFKVITSSTEAQGFTPGSFIAGGSWLKATTFPFSLGL 319
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 386.726 bits (992), Expect = 8.540e-107
Identity = 183/221 (82.81%), Postives = 199/221 (90.05%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDITFQNTAGPSKHQAVALRVG+D SAFYNCDMLAYQDTLYVHSNRQFFV CLIAGTVDFIFGNAA VLQ+CDIHAR+PNSGQKNMVTAQGRTDPNQNTGIVIQK RIGATSDL+ V+GSFPTYLGRPWKEYS+TVIMQS+I+D+I P GW EW G FALNTL Y E+ N+GAGAGT+ RVKW+GF+VIT+A EAQ +T G FI G  WL STGFPFSLGL
Sbjct:  372 RDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 370.163 bits (949), Expect = 8.271e-102
Identity = 176/221 (79.64%), Postives = 191/221 (86.43%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDITFQNTAGPSKHQAVALRVG+D SAFY CDM AYQDTLYVHSNRQFFV C I GTVDFIFGNAAAVLQ+CDI+AR+PNSGQKNMVTAQGR+DPNQNTGIVIQ  RIG TSDL  V+G+FPTYLGRPWKEYSRTVIMQS I+D+I P GWHEW G+FAL+TL Y E+ N G GAGT+ RVKWKG++VITS TEAQ FT G FI G  WL STGFPFSL L
Sbjct:  367 RDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 302.753 bits (774), Expect = 1.622e-81
Identity = 148/221 (66.97%), Postives = 174/221 (78.73%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            R ITF+NTAG S  QAVALR G+DLS FY C   AYQDTLYVHSNRQF+ +C + GTVDFIFGNAAAVLQNC+I AR+P S + N +TAQGR+DPNQNTGI+I  SR+ A SDL+PV GS  TYLGRPW++YSRTV M++S+  LI P GW EWDGNFAL TLFY E QN+G GA TSGRV W GFRV+ SA+EA  FT G+F+AG SW+ S+  PF+ GL
Sbjct:  293 RGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPRS-KTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 1.518e-79
Identity = 139/221 (62.90%), Postives = 172/221 (77.83%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDIT +NTAGP+ HQAVALR G+DLS FY C    YQDTLYVHS RQF+  C I GTVDFIFGNAA VLQNC+I ARKP + + N +TAQGRTDPNQ+TGI+I   R+ A SDLKPVQ S  T+LGRPWK+YSRTV +++ +  LI+PAGW EW G+FALNTL+Y E+ N+G G+ T+ RVKW+G+ V+TS ++   FT G+FIAG+SWL +T  PF+ GL
Sbjct:  291 RDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME_ACTDE (Pectinesterase OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 287.345 bits (734), Expect = 7.051e-77
Identity = 137/221 (61.99%), Postives = 162/221 (73.30%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            R I+F+N AGPSKHQAVALR GAD SAFY C  + YQDTLYVHS RQF+  C + GT+DFIFGNAAAVLQ C+++ARKPN  QKN+ TAQGR DPNQNTGI I   ++ A +DL PV  SF TYLGRPWKEYSRTV + S +  LI PAGW EW G+FAL TL+Y E++N+G G+ T+ RV W G+ V T+ TE   FT G+FI GS WL S   P  L L
Sbjct:  100 RGISFENYAGPSKHQAVALRSGADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNENQKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQMESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGNFIQGSQWLTSYNIPVYLNL 320
[Close]
BLAST of CF836623 vs. ExPASy Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 9.209e-77
Identity = 141/221 (63.80%), Postives = 160/221 (72.40%), Query Frame = -3 
Query:  181 RDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 843
            RDITF+N AGP+KHQAVALR+GAD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIFGNAA VLQNC I+ARKP   QKN +TAQ R DPNQNTGI I  SR+ A SDL+   GS  TYLGRPWK +SRTV M S I   +H  GW EW+  FAL+TL+YGE+ NSG G+G   RV W G+RVI S  EA  FT   FI GSSWL STG  F  GL
Sbjct:  376 RDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596
The following BLAST results are available for this feature:
BLAST of CF836623 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 87
Match NameE-valueIdentityDescription
PME1_CITSI2.309e-128100.00Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE... [more]
PME3_CITSI4.355e-12798.64Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PMEU1_SOLLC1.504e-11186.88Pectinesterase/pectinesterase inhibitor U1 OS=Sola... [more]
PME_DAUCA1.663e-11085.07Pectinesterase OS=Daucus carota PE=1 SV=1[more]
PME3_ARATH8.540e-10782.81Pectinesterase/pectinesterase inhibitor 3 OS=Arabi... [more]
PME2_ARATH8.271e-10279.64Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 P... [more]
PME17_ARATH1.622e-8166.97Probable pectinesterase/pectinesterase inhibitor 1... [more]
PME2_CITSI1.518e-7962.90Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE... [more]
PME_ACTDE7.051e-7761.99Pectinesterase OS=Actinidia deliciosa PE=1 SV=1[more]
PME34_ARATH9.209e-7763.80Probable pectinesterase/pectinesterase inhibitor 3... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS03_03E17_f Washington Navel Orange Shoot Meristem cDNA Library Citrus sinensis cDNA clone CS_PEa03E17, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF836623 ID=CF836623; Name=CF836623; organism=Citrus sinensis; type=EST; length=845bp
TTTTTTTTTTTTTTTATTATTCTTCTCATGCTATTTTATTTCTTGCAAAT
CACACAATAGTATTTGTGAGATCGAAGCCACGAATAAGACACGCTCAATC
AAATATTACAAATTGAAAGACCCGAGATCGATGGATCCACTAATACAAAA
CATATTAATTAAAACTCCTAGTGAATATTACAAACCAAGGGAGAATGGGA
AACCAGTGGAGCCCAGCCAGCTACTACCAGCAATGAAGCTTCCAGGAGTA
AAAGCTTGAGCCTCGGTAGCACTTGTAATAACCCTAAATCCCTTCCATTT
CACTCTCCCTGAAGTTCCGGCACCGGCTCCGCTGTTCTGATGCTCTCCGT
AAAACAATGTGTTCAACGCGAAGTTACCATCCCACTCGTGCCACCCGGCA
GGGTGGATCAAGTCAGTAATCGACGACTGCATGATCACCGTCCTCGAGTA
CTCCTTCCAGGGCCTGCCGAGGTACGTCGGGAAACTACCCTGAACCGGTT
TTAAATCGGAGGTGGCACCAATCCTAGATTTTTGAATGACAATGCCGGTG
TTTTGGTTAGGGTCAGTCCTGCCTTGGGCTGTGACCATATTTTTTTGGCC
GGAATTGGGCTTTCGTGCATGGATGTCACAATTTTGTAACACGGCTGCAG
CATTACCAAAAATGAAATCAACCGTGCCAGCAATTAAGCAGTTCACAAAG
AACTGGCGGTTCGAGTGGACGTAGAGTGTGTCTTGGTAAGCTAACATATC
GCAATTGTAAAATGCTGAAAGGTCAGCTCCCACTCGCAGTGCCACTGCCT
GGTGCTTTGAGGGGCCTGCTGTGTTTTGGAATGTAATGTCTCGGG
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