CK739456

Overview
NameCK739456
Unique NameCK739456
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length365
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXB1_DICDI (Beta-hexosaminidase subunit B1 OS=Dictyostelium discoideum GN=hexb1 PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 9.411e-25
Identity = 34/64 (53.12%), Postives = 44/64 (68.75%), Query Frame = 1 
Query:  148 SLHWHIIDEQSFPLEVPTYPNLWKGAYSKWERYTVEDAHEIVGFAKMRGINVMAEVDVPGHAES 339
            +LHWHIID QSFPL   +YPNL  GA+SK E Y+  D   I+ + K  GI +  E+D+PGHA+S
Sbjct:  216 TLHWHIIDSQSFPLSSKSYPNLINGAWSKSEIYSYHDIKRIIKYGKENGIRIQLEIDMPGHAKS 279

HSP 2 Score: 55.0694 bits (131), Expect = 9.411e-25
Identity = 24/42 (57.14%), Postives = 31/42 (73.81%), Query Frame = 3 
Query:   21 PWYIQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLN 146
            P  I DKPRF  RG+++DTSRH+  VD I ++IES+SY K N
Sbjct:  174 PLIINDKPRFPHRGVMLDTSRHFYSVDTILKVIESLSYNKFN 215
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXB_RAT (Beta-hexosaminidase subunit beta OS=Rattus norvegicus GN=Hexb PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.151e-21
Identity = 32/64 (50.00%), Postives = 44/64 (68.75%), Query Frame = 1 
Query:  151 LHWHIIDEQSFPLEVPTYPNLW-KGAYSKWERYTVEDAHEIVGFAKMRGINVMAEVDVPGHAES 339
            LHWHI+D+QSFP +  T+P L  KG+YS    YT  D H ++ +A++RGI V+ E D PGH +S
Sbjct:  212 LHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDIHMVLEYARLRGIRVIPEFDSPGHTQS 275

HSP 2 Score: 51.9878 bits (123), Expect = 1.151e-21
Identity = 23/40 (57.50%), Postives = 30/40 (75.00%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I D PRF  RG+LIDTSRHYLPV  I + +++M++ K NV
Sbjct:  172 IADSPRFPHRGILIDTSRHYLPVKTIFKTLDAMAFNKFNV 211
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXB_HUMAN (Beta-hexosaminidase subunit beta OS=Homo sapiens GN=HEXB PE=1 SV=3)

HSP 1 Score: 67.781 bits (164), Expect = 2.532e-21
Identity = 31/64 (48.44%), Postives = 42/64 (65.62%), Query Frame = 1 
Query:  151 LHWHIIDEQSFPLEVPTYPNLW-KGAYSKWERYTVEDAHEIVGFAKMRGINVMAEVDVPGHAES 339
            LHWHI+D+QSFP +  T+P L  KG+YS    YT  D   ++ +A++RGI V+ E D PGH  S
Sbjct:  234 LHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLS 297

HSP 2 Score: 53.5286 bits (127), Expect = 2.532e-21
Identity = 23/40 (57.50%), Postives = 32/40 (80.00%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I D PRF+ RG+LIDTSRHYLPV +I + +++M++ K NV
Sbjct:  194 IIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNV 233
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXB_FELCA (Beta-hexosaminidase subunit beta OS=Felis catus GN=HEXB PE=2 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 4.287e-21
Identity = 32/64 (50.00%), Postives = 44/64 (68.75%), Query Frame = 1 
Query:  151 LHWHIIDEQSFPLEVPTYPNLW-KGAYSKWERYTVEDAHEIVGFAKMRGINVMAEVDVPGHAES 339
            LHWHI+D+QSFP +  T+P L  KG+YS    YT  D H ++ +A++RGI V+ E D PGH +S
Sbjct:  208 LHWHIVDDQSFPYQSVTFPELSNKGSYSLSHVYTPNDVHTVIEYARLRGIRVIPEFDSPGHTQS 271

HSP 2 Score: 48.1358 bits (113), Expect = 4.287e-21
Identity = 21/40 (52.50%), Postives = 30/40 (75.00%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I D PRF  RG+LIDT+RH+LPV  I + +++M++ K NV
Sbjct:  168 IIDSPRFPHRGILIDTARHFLPVKSILKTLDAMAFNKFNV 207
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXB_MOUSE (Beta-hexosaminidase subunit beta OS=Mus musculus GN=Hexb PE=2 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 5.576e-21
Identity = 31/64 (48.44%), Postives = 43/64 (67.19%), Query Frame = 1 
Query:  151 LHWHIIDEQSFPLEVPTYPNLW-KGAYSKWERYTVEDAHEIVGFAKMRGINVMAEVDVPGHAES 339
            LHWHI+D+QSFP +  T+P L  KG+YS    YT  D   ++ +A++RGI V+ E D PGH +S
Sbjct:  213 LHWHIVDDQSFPYQSTTFPELSNKGSYSLSHVYTPNDVRMVLEYARLRGIRVIPEFDTPGHTQS 276

HSP 2 Score: 50.8322 bits (120), Expect = 5.576e-21
Identity = 22/40 (55.00%), Postives = 30/40 (75.00%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I D PRF  RG+LIDTSRH+LPV  I + +++M++ K NV
Sbjct:  173 IADSPRFPHRGILIDTSRHFLPVKTILKTLDAMAFNKFNV 212
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXA1_DICDI (Beta-hexosaminidase subunit A1 OS=Dictyostelium discoideum GN=hexa1 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.077e-20
Identity = 30/62 (48.39%), Postives = 44/62 (70.97%), Query Frame = 1 
Query:  148 SLHWHIIDEQSFPLEVPTYPNLWKGAYSKWERYTVEDAHEIVGFAKMRGINVMAEVDVPGHA 333
            +LHWH++D  +FP+E  TYP+L KGA+S    ++ +D  E+V +AK  GI V+ E D+PGHA
Sbjct:  188 TLHWHMVDAVAFPVESTTYPDLTKGAFSPSATFSHDDIQEVVAYAKTYGIRVIPEFDIPGHA 249

HSP 2 Score: 46.2098 bits (108), Expect = 2.077e-20
Identity = 16/39 (41.03%), Postives = 30/39 (76.92%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLN 146
            I D PR+ +RG ++D++RHY+P ++I  +I+S+ ++K N
Sbjct:  149 ISDSPRYPWRGFMVDSARHYIPKNMILHMIDSLGFSKFN 187
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXA_BOVIN (Beta-hexosaminidase subunit alpha OS=Bos taurus GN=HEXA PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.730e-20
Identity = 31/67 (46.27%), Postives = 44/67 (65.67%), Query Frame = 1 
Query:  154 HWHIIDEQSFPLEVPTYPNLWK-GAYSKWER-YTVEDAHEIVGFAKMRGINVMAEVDVPGHAES*EP 348
            HWH++D+ SFP E  T+P L K G+Y+     YT +D  E++ +A++RGI V+AE D PGH  S  P
Sbjct:  202 HWHLVDDSSFPYESFTFPELTKKGSYNPATHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGP 268

HSP 2 Score: 50.0618 bits (118), Expect = 7.730e-20
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I+D PRF  RGLL+DTSRHYLP+  I   ++ M+Y K NV
Sbjct:  161 IEDFPRFPHRGLLLDTSRHYLPLASILDTLDVMAYNKFNV 200
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXA_MOUSE (Beta-hexosaminidase subunit alpha OS=Mus musculus GN=Hexa PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.740e-19
Identity = 30/67 (44.78%), Postives = 44/67 (65.67%), Query Frame = 1 
Query:  154 HWHIIDEQSFPLEVPTYPNLW-KGAYSKWER-YTVEDAHEIVGFAKMRGINVMAEVDVPGHAES*EP 348
            HWH++D+ SFP E  T+P L  KG+++     YT +D  E++ +A++RGI V+AE D PGH  S  P
Sbjct:  202 HWHLVDDSSFPYESFTFPELTRKGSFNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGP 268

HSP 2 Score: 49.2914 bits (116), Expect = 3.740e-19
Identity = 22/40 (55.00%), Postives = 29/40 (72.50%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I+D PRF  RG+L+DTSRHYLP+  I   ++ M+Y K NV
Sbjct:  161 IKDFPRFPHRGVLLDTSRHYLPLSSILDTLDVMAYNKFNV 200
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXA_RAT (Beta-hexosaminidase subunit alpha OS=Rattus norvegicus GN=Hexa PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 6.324e-19
Identity = 29/64 (45.31%), Postives = 43/64 (67.19%), Query Frame = 1 
Query:  154 HWHIIDEQSFPLEVPTYPNLW-KGAYSKWER-YTVEDAHEIVGFAKMRGINVMAEVDVPGHAES 339
            HWH++D+ SFP E  T+P L  KG+++     YT +D  E++ +A++RGI V+AE D PGH  S
Sbjct:  202 HWHLVDDSSFPYESFTFPELTRKGSFNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLS 265

HSP 2 Score: 48.9062 bits (115), Expect = 6.324e-19
Identity = 22/40 (55.00%), Postives = 28/40 (70.00%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I D PRF  RG+L+DTSRHYLP+  I   ++ M+Y K NV
Sbjct:  161 ITDFPRFPHRGILLDTSRHYLPLSSILNTLDVMAYNKFNV 200
[Close]
BLAST of CK739456 vs. ExPASy Swiss-Prot
Match: HEXB_PIG (Beta-hexosaminidase subunit beta OS=Sus scrofa GN=HEXB PE=1 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 3.244e-17
Identity = 30/64 (46.88%), Postives = 41/64 (64.06%), Query Frame = 1 
Query:  151 LHWHIIDEQSFPLEVPTYPNLW-KGAYSKWERYTVEDAHEIVGFAKMRGINVMAEVDVPGHAES 339
            LHWHI+D+QSFP +   +  L  KG+YS    YT  D   ++ +A++RGI VM E D PGH+ S
Sbjct:  208 LHWHIVDDQSFPYQSINFGVLSSKGSYSLSHVYTPNDVRMVIEYARIRGIRVMPEFDTPGHSRS 271

HSP 2 Score: 43.8986 bits (102), Expect = 3.244e-17
Identity = 20/40 (50.00%), Postives = 28/40 (70.00%), Query Frame = 3 
Query:   30 IQDKPRFAFRGLLIDTSRHYLPVDVIKQIIESMSYAKLNV 149
            I D PRF  RG+LIDT RH+L V  I + +++M++ K NV
Sbjct:  168 IIDFPRFPHRGILIDTGRHFLSVKTIFKTLDAMAFNKFNV 207
The following BLAST results are available for this feature:
BLAST of CK739456 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19
Match NameE-valueIdentityDescription
HEXB1_DICDI9.411e-2553.13Beta-hexosaminidase subunit B1 OS=Dictyostelium di... [more]
HEXB_RAT1.151e-2150.00Beta-hexosaminidase subunit beta OS=Rattus norvegi... [more]
HEXB_HUMAN2.532e-2148.44Beta-hexosaminidase subunit beta OS=Homo sapiens G... [more]
HEXB_FELCA4.287e-2150.00Beta-hexosaminidase subunit beta OS=Felis catus GN... [more]
HEXB_MOUSE5.576e-2148.44Beta-hexosaminidase subunit beta OS=Mus musculus G... [more]
HEXA1_DICDI2.077e-2048.39Beta-hexosaminidase subunit A1 OS=Dictyostelium di... [more]
HEXA_BOVIN7.730e-2046.27Beta-hexosaminidase subunit alpha OS=Bos taurus GN... [more]
HEXA_MOUSE3.740e-1944.78Beta-hexosaminidase subunit alpha OS=Mus musculus ... [more]
HEXA_RAT6.324e-1945.31Beta-hexosaminidase subunit alpha OS=Rattus norveg... [more]
HEXB_PIG3.244e-1746.88Beta-hexosaminidase subunit beta OS=Sus scrofa GN=... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUSDA-FP_5918 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE56F10 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK739456 ID=CK739456; Name=CK739456; organism=Citrus sinensis; type=EST; length=365bp
CCGTACTAGTATATAAAGCACCGTGGTACATCCAAGACAAACCAAGATTT
GCATTTCGTGGCCTTCTGATTGATACATCAAGGCACTATTTACCAGTAGA
TGTCATTAAGCAGATAATTGAATCTATGTCATATGCAAAACTTAATGTCC
CTTCATTGGCATATCATAGACGAGCAGTCATTTCCTCTTGAAGTGCCTAC
ATATCCAAATTTGTGGAAGGGCGCATATTCAAAGTGGGAGCGCTACACGG
TTGAGGATGCCCATGAAATTGTCGGTTTCGCCAAGATGAGAGGCATCAAT
GTTATGGCAGAAGTAGATGTCCCTGGTCATGCCGAATCATGAGAGCCACT
TGATGTTTCAAAAAT
back to top